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Protein

Peroxidase 40

Gene

PER40

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei86 – 861Transition state stabilizerPROSITE-ProRule annotation
Active sitei90 – 901Proton acceptorPROSITE-ProRule annotation
Metal bindingi91 – 911Calcium 1PROSITE-ProRule annotation
Metal bindingi94 – 941Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi96 – 961Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi98 – 981Calcium 1PROSITE-ProRule annotation
Metal bindingi100 – 1001Calcium 1PROSITE-ProRule annotation
Binding sitei187 – 1871Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi217 – 2171Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi218 – 2181Calcium 2PROSITE-ProRule annotation
Metal bindingi269 – 2691Calcium 2PROSITE-ProRule annotation
Metal bindingi272 – 2721Calcium 2PROSITE-ProRule annotation
Metal bindingi277 – 2771Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G16270-MONOMER.

Protein family/group databases

PeroxiBasei206. AtPrx40.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 40 (EC:1.11.1.7)
Short name:
Atperox P40
Gene namesi
Name:PER40
Synonyms:P40
Ordered Locus Names:At4g16270
ORF Names:dl4175w, FCAALL.329
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G16270.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 348327Peroxidase 40PRO_0000023706Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi59 ↔ 139PROSITE-ProRule annotation
Disulfide bondi92 ↔ 97PROSITE-ProRule annotation
Disulfide bondi145 ↔ 344PROSITE-ProRule annotation
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence analysis
Disulfide bondi224 ↔ 256PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO23474.
PRIDEiO23474.

Expressioni

Gene expression databases

GenevisibleiO23474. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G16270.1.

Structurei

3D structure databases

ProteinModelPortaliO23474.
SMRiO23474. Positions 54-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHXA. Eukaryota.
ENOG410YEGN. LUCA.
HOGENOMiHOG000237557.
InParanoidiO23474.
KOiK00430.
OrthoDBiEOG09360IR9.
PhylomeDBiO23474.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23474-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLFNLFLM FFFAMPILSL SENPTNFSES CEDGSGETGS SFGIGFDLVL
60 70 80 90 100
DFGLYRNSCP EAESIVYSWV ETTVLEDPRM AASLLRLHFH DCFVNGCDAS
110 120 130 140 150
VLLDDTEGLV GEKTAPPNLN SLRGFEVIDS IKSDIESVCP ETVSCADILA
160 170 180 190 200
MAARDSVVVS GGPRWEVEVG RKDSRTASKQ AATNGLPSPN STVSTLISTF
210 220 230 240 250
QNLGLSQTDM VALSGGHTLG KARCTSFTAR LQPLQTGQPA NHGDNLEFLE
260 270 280 290 300
SLQQLCSTVG PSVGITQLDL VTPSTFDNQY YVNLLSGEGL LPSDQALAVQ
310 320 330 340
DPGTRAIVET YATDQSVFFE DFKNAMVKMG GIPGGSNSEI RKNCRMIN
Length:348
Mass (Da):37,460
Last modified:December 6, 2002 - v2
Checksum:i1B438813818178E5
GO

Sequence cautioni

The sequence AEE83724 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAD43424 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAD43745 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAB10406 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAB78669 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97340 Genomic DNA. Translation: CAB10406.1. Sequence problems.
AL161543 Genomic DNA. Translation: CAB78669.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83724.1. Different initiation.
BT011747 mRNA. Translation: AAS49110.1.
AK175661 mRNA. Translation: BAD43424.1. Different initiation.
AK175982 mRNA. Translation: BAD43745.1. Different initiation.
RefSeqiNP_193362.2. NM_117724.3.
UniGeneiAt.50347.
At.67828.

Genome annotation databases

EnsemblPlantsiAT4G16270.1; AT4G16270.1; AT4G16270.
GeneIDi827322.
GrameneiAT4G16270.1; AT4G16270.1; AT4G16270.
KEGGiath:AT4G16270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97340 Genomic DNA. Translation: CAB10406.1. Sequence problems.
AL161543 Genomic DNA. Translation: CAB78669.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83724.1. Different initiation.
BT011747 mRNA. Translation: AAS49110.1.
AK175661 mRNA. Translation: BAD43424.1. Different initiation.
AK175982 mRNA. Translation: BAD43745.1. Different initiation.
RefSeqiNP_193362.2. NM_117724.3.
UniGeneiAt.50347.
At.67828.

3D structure databases

ProteinModelPortaliO23474.
SMRiO23474. Positions 54-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G16270.1.

Protein family/group databases

PeroxiBasei206. AtPrx40.

Proteomic databases

PaxDbiO23474.
PRIDEiO23474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G16270.1; AT4G16270.1; AT4G16270.
GeneIDi827322.
GrameneiAT4G16270.1; AT4G16270.1; AT4G16270.
KEGGiath:AT4G16270.

Organism-specific databases

TAIRiAT4G16270.

Phylogenomic databases

eggNOGiENOG410IHXA. Eukaryota.
ENOG410YEGN. LUCA.
HOGENOMiHOG000237557.
InParanoidiO23474.
KOiK00430.
OrthoDBiEOG09360IR9.
PhylomeDBiO23474.

Enzyme and pathway databases

BioCyciARA:AT4G16270-MONOMER.

Miscellaneous databases

PROiO23474.

Gene expression databases

GenevisibleiO23474. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER40_ARATH
AccessioniPrimary (citable) accession number: O23474
Secondary accession number(s): F4JLR5, Q53XE3, Q67ZY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.