O23463 (CMTA5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calmodulin-binding transcription activator 5

Alternative name(s):
Ethylene-induced calmodulin-binding protein f
Short name=EICBP.f
Signal-responsive protein 6

Gene names

Name:	CMTA5
Synonyms:	SR6
Ordered Locus Names:	At4g16150
ORF Names:	dl4115W, FCAALL.291

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulates transcriptional activity in response to calcium signals. Binds to the consensus sequence 5'-[ACG]CGCG[GTC]-3'. Activates the expression of the V-PPase proton pump in pollen. Ref.5 Ref.6 Ref.7
Subunit structure	Binds to calmodulin.
Subcellular location	Nucleus Ref.5 Ref.6 Ref.7.
Tissue specificity	Expressed in roots, stems, leaves, pollen, top of sepals and siliques. Ref.6 Ref.7
Induction	By temperature, UVB, wounding, abscisic acid, H2O2 and salicylic acid. Ref.6
Sequence similarities	Belongs to the CAMTA family. Contains 1 ANK repeat. Contains 1 CG-1 DNA-binding domain. Contains 4 IQ domains.
Sequence caution	The sequence CAB10394.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB10394.1 differs from that shown. Reason: Frameshift at positions 58, 382 and 635. The sequence CAB78657.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB78657.1 differs from that shown. Reason: Frameshift at positions 58, 382 and 635.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Cellular component	Nucleus
Domain	ANK repeat Coiled coil Repeat
Ligand	Calcium Calmodulin-binding DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Traceable author statement Ref.6. Source: TAIR response to stress Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 923	923	Calmodulin-binding transcription activator 5		PRO_0000114490
Regions
	Repeat	611 – 640	30	ANK
	Domain	757 – 786	30	IQ 1
	Domain	799 – 828	30	IQ 2
	Domain	875 – 904	30	IQ 3
	DNA binding	25 – 151	127	CG-1
	Coiled coil	887 – 914	28	Potential
	Compositional bias	86 – 89	4	Poly-Lys

Sequences

	Sequence		Length	Mass (Da)
	O23463 [UniParc]. Last modified January 24, 2006. Version 2. Checksum: AAA73F4A14E9A5DA	FASTA	923	104,847
	10 20 30 40 50 60 MAGVDSGKLI GSEIHGFHTL QDLDIQTMLD EAYSRWLRPN EIHALLCNHK FFTINVKPVN 70 80 90 100 110 120 LPKSGTIVLF DRKMLRNFRK DGHNWKKKKD GKTIKEAHEH LKVGNEERIH VYYAHGEDTP 130 140 150 160 170 180 TFVRRCYWLL DKSQEHIVLV HYRETHEVHA APATPGNSYS SSITDHLSPK IVAEDTSSGV 190 200 210 220 230 240 HNTCNTGFEV RSNSLGSRNH EIRLHEINTL DWDELLVPAD ISNQSHPTEE DMLYFTEQLQ 250 260 270 280 290 300 TAPRGSVKQG NHLAGYNGSV DIPSFPGLED PVYQNNNSCG AGEFSSQHSH CGVDPNLQRR 310 320 330 340 350 360 DFSATVTDQP GDALLNNGYG SQDSFGRWVN NFISDSPGSV DDPSLEAVYT PGQDSSTPPT 370 380 390 400 410 420 VFHSHSDIPE QVFNITDVSP AWAYSTEKTK ILVTGFFHDS FQHLGRSNLI CICGELRVPA 430 440 450 460 470 480 EFLQMGVYRC FLPPQSPGVV NLYLSVDGNK PISQLFSFEH RSVQFIEKAI PQDDQLYKWE 490 500 510 520 530 540 EFEFQVRLAH LLFTSSNKIS VLTSKISPEN LLEAKKLASR TSHLLNSWAY LMKSIQANEV 550 560 570 580 590 600 PFDQARDHLF ELTLKNRLKE WLLEKVIENR NTKEYDSKGL GVIHLCAVLG YTWSILLFSW 610 620 630 640 650 660 ANISLDFRDK QGWTALHWAA YYGREKMVAA LLSAGARPNL VTDPTKEFLG GCTAADLAQQ 670 680 690 700 710 720 KGYDGLAAFL AEKCLVAQFK DMQTAGNISG NLETIKAEKS SNPGNANEEE QSLKDTLAAY 730 740 750 760 770 780 RTAAEAAARI QGAFREHELK VRSSAVRFAS KEEEAKNIIA AMKIQHAFRN FEVRRKIAAA 790 800 810 820 830 840 ARIQYRFQTW KMRREFLNMR KKAIRIQAAF RGFQVRRQYQ KITWSVGVLE KAILRWRLKR 850 860 870 880 890 900 KGFRGLQVSQ PDEKEGSEAV EDFYKTSQKQ AEERLERSVV KVQAMFRSKK AQQDYRRMKL 910 920 AHEEAQLEYD GMQELDQMAT EES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"A novel family of calmodulin-binding transcription activators in multicellular organisms." Bouche N., Scharlat A., Snedden W., Bouchez D., Fromm H. J. Biol. Chem. 277:21851-21861(2002) [PubMed: 11925432] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants." Yang T., Poovaiah B.W. J. Biol. Chem. 277:45049-45058(2002) [PubMed: 12218065] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[7]	"Arabidopsis CAMTA family proteins enhance V-PPase expression in pollen." Mitsuda N., Isono T., Sato M.H. Plant Cell Physiol. 44:975-981(2003) [PubMed: 14581622] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[8]	"Genes encoding calmodulin-binding proteins in the Arabidopsis genome." Reddy V.S., Ali G.S., Reddy A.S.N. J. Biol. Chem. 277:9840-9852(2002) [PubMed: 11782485] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z97340 Genomic DNA. Translation: CAB10394.1. Sequence problems. AL161543 Genomic DNA. Translation: CAB78657.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE83703.1. AY128295 mRNA. Translation: AAM91103.1.
IPI	IPI00532850.
PIR	H71427.
RefSeq	NP_193350.5. NM_117710.6.
UniGene	At.20170.
3D structure databases
ProteinModelPortal	O23463.
SMR	O23463. Positions 373-462, 568-694, 774-822.
ModBase	Search...
Protein-protein interaction databases
STRING	O23463.
Proteomic databases
PRIDE	O23463.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G16150.1; AT4G16150.1; AT4G16150.
GeneID	827305.
GenomeReviews	Gene locus AT4G16150 in contig CT486007_GR.
KEGG	ath:AT4G16150.
Organism-specific databases
TAIR	At4g16150.
Phylogenomic databases
eggNOG	KOG0520.
GeneTree	EPGT00050000004552.
HOGENOM	HBG599760.
PhylomeDB	O23463.
ProtClustDB	CLSN2684182.
Gene expression databases
ArrayExpress	O23463.
Genevestigator	O23463.
GermOnline	AT4G16150. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005559. CG-1_dom. IPR014756. Ig_E-set. IPR000048. IQ_motif_EF-hand-BS. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 1 hit. PF03859. CG-1. 1 hit. PF00612. IQ. 2 hits. [Graphical view]
SMART	SM00248. ANK. 1 hit. SM01076. CG-1. 1 hit. SM00015. IQ. 3 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF81296. Ig_E-set. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. PS51437. CG_1. 1 hit. PS50096. IQ. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMTA5_ARATH

Accession

Primary (citable) accession number: O23463
Secondary accession number(s): Q8L7R9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program