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Protein

L-arabinokinase

Gene

ARA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Arabinose kinase. Involved in the salvage pathway which converts free L-arabinose to UDP-L-arabinose. May play a role in arabinose transport.1 Publication

Catalytic activityi

ATP + L-arabinose = ADP + beta-L-arabinose 1-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei557 – 5571Transition state stabilizerBy similarity
Active sitei745 – 7451Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi693 – 70311ATPSequence analysisAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • L-arabinokinase activity Source: TAIR

GO - Biological processi

  • carbohydrate phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G16130-MONOMER.
MetaCyc:AT4G16130-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-arabinokinase (EC:2.7.1.46)
Short name:
AtISA1
Gene namesi
Name:ARA1
Synonyms:ISA1
Ordered Locus Names:At4g16130
ORF Names:dl4105w, FCAALL.288
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G16130.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei662 – 67817HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi705 – 7051E → K in ara1-1; arabinose sensitivity and loss of kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10391039L-arabinokinasePRO_0000407404Add
BLAST

Proteomic databases

PaxDbiO23461.
PRIDEiO23461.

PTM databases

iPTMnetiO23461.

Expressioni

Gene expression databases

GenevisibleiO23461. AT.

Interactioni

Protein-protein interaction databases

BioGridi12593. 4 interactions.
IntActiO23461. 3 interactions.
STRINGi3702.AT4G16130.1.

Structurei

3D structure databases

ProteinModelPortaliO23461.
SMRiO23461. Positions 536-822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 124Poly-Lys
Compositional biasi21 – 244Poly-Leu

Sequence similaritiesi

Belongs to the GHMP kinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
HOGENOMiHOG000005811.
InParanoidiO23461.
KOiK12446.
OMAiICQPAEV.
PhylomeDBiO23461.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR012369. Galk_glycosyltransferase.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036399. Gal_kin_glcsltr. 1 hit.
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 2 hits.
SSF55060. SSF55060. 3 hits.

Sequencei

Sequence statusi: Complete.

O23461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERAKLQKK KKVTEDLLDS LLLLPPWRNF AISQSHQSII ESSSQRLPEK
60 70 80 90 100
MRIDENEGVS ASSKHLVFAY YVTGHGFGHA TRVVEVVRHL IAAGHDVHVV
110 120 130 140 150
TGAPDFVFTS EIQSPRLKIR KVLLDCGAVQ ADALTVDRLA SLEKYVETAV
160 170 180 190 200
VPRAEILETE VEWLHSIKAD FVVSDVVPVA CRAAADAGIR SVCVTNFSWD
210 220 230 240 250
FIYAEYVMAA GYHHRSIVWQ IAEDYSHCEF LIRLPGYCPM PAFRDVIDVP
260 270 280 290 300
LVVRRLHKSR KEVRKELGIA EDVNVVILNF GGQPSGWNLK ETSLPTGWLC
310 320 330 340 350
LVCGASETLE LPPNFIKLAK DAYTPDIIAA SDCMLGKIGY GTVSEALSYK
360 370 380 390 400
VPFVFVRRDY FNEEPFLRNM LEFYQCGVEM IRRDLLMGQW TPYLERAVSL
410 420 430 440 450
KPCYEGGING GEIAAHILQE TAIGRHCASD KLSGARRLRD AIILGYQLQR
460 470 480 490 500
VPGRDIAIPE WYSRAENELG QSAGSSPTVQ ANENNSLVES CIDDFDILQG
510 520 530 540 550
DVQGLSDTCT FLKSLAMLDA IHDSEKSTEK KTVRERKAAG GLFNWEEEIF
560 570 580 590 600
VARAPGRLDV MGGIADYSGS LVLQMPIREA CHVAVQRNLP GKHRLWKHAQ
610 620 630 640 650
ARQQAKGQVP TPVLQIVSYG SEISNRAPTF DMDLSDFMDG DEPISYEKAR
660 670 680 690 700
KFFAQDPAQK WAAYVAGTIL VLMIELGVRF EDSISLLVSS AVPEGKGVSS
710 720 730 740 750
SAAVEVASMS AIAAAHGLSI DPRDLAILCQ KVENHIVGAP CGVMDQMTSS
760 770 780 790 800
CGEANKLLAM ICQPAEVVGL VEIPNHVRFW GIDSGIRHSV GGADYRSVRV
810 820 830 840 850
GAYMGRKMIK SMASSILSPS ASSANGGNPE ELEDEGIDLL EAEASLDYLC
860 870 880 890 900
NLSPHRYEAR YADKLPDIML GQTFIEEYAD HDDPVTVIDQ KRSYSVKAPA
910 920 930 940 950
RHPIYENFRV KTFKALLTSA TSDEQLTALG GLLYQCHYSY SACGLGSDGT
960 970 980 990 1000
NRLVQLVQGM QHNKSNSEDG TLYGAKITGG GSGGTVCVVG RNSLRSSQQI
1010 1020 1030
LEIQQRYKAA TGYLPLIFEG SSPGAGKFGY LRIRRRISL
Length:1,039
Mass (Da):114,261
Last modified:January 1, 1998 - v1
Checksum:i82843127FCCB66B5
GO

Sequence cautioni

The sequence BT003908 differs from that shown. Reason: Frameshift at position 180. Curated
The sequence CAA74753.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441K → E in BT003908 (PubMed:14593172).Curated
Sequence conflicti585 – 5851V → A in CAA74753 (PubMed:9524266).Curated
Sequence conflicti681 – 6811E → G in BAH20381 (PubMed:19423640).Curated
Sequence conflicti890 – 8901Q → E in CAA74753 (PubMed:9524266).Curated
Sequence conflicti961 – 9611Q → R in BT003908 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97340 Genomic DNA. Translation: CAB10392.1.
AL161543 Genomic DNA. Translation: CAB78655.1.
CP002687 Genomic DNA. Translation: AEE83696.1.
BT003908 mRNA. No translation available.
Y14404 Genomic DNA. Translation: CAA74753.1. Different initiation.
AK317724 mRNA. Translation: BAH20381.1.
AK230073 mRNA. Translation: BAF01893.1.
PIRiF71427.
RefSeqiNP_193348.1. NM_117706.2.
UniGeneiAt.218.
At.67863.

Genome annotation databases

EnsemblPlantsiAT4G16130.1; AT4G16130.1; AT4G16130.
GeneIDi827299.
GrameneiAT4G16130.1; AT4G16130.1; AT4G16130.
KEGGiath:AT4G16130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97340 Genomic DNA. Translation: CAB10392.1.
AL161543 Genomic DNA. Translation: CAB78655.1.
CP002687 Genomic DNA. Translation: AEE83696.1.
BT003908 mRNA. No translation available.
Y14404 Genomic DNA. Translation: CAA74753.1. Different initiation.
AK317724 mRNA. Translation: BAH20381.1.
AK230073 mRNA. Translation: BAF01893.1.
PIRiF71427.
RefSeqiNP_193348.1. NM_117706.2.
UniGeneiAt.218.
At.67863.

3D structure databases

ProteinModelPortaliO23461.
SMRiO23461. Positions 536-822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12593. 4 interactions.
IntActiO23461. 3 interactions.
STRINGi3702.AT4G16130.1.

PTM databases

iPTMnetiO23461.

Proteomic databases

PaxDbiO23461.
PRIDEiO23461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G16130.1; AT4G16130.1; AT4G16130.
GeneIDi827299.
GrameneiAT4G16130.1; AT4G16130.1; AT4G16130.
KEGGiath:AT4G16130.

Organism-specific databases

TAIRiAT4G16130.

Phylogenomic databases

eggNOGiKOG0631. Eukaryota.
COG0153. LUCA.
HOGENOMiHOG000005811.
InParanoidiO23461.
KOiK12446.
OMAiICQPAEV.
PhylomeDBiO23461.

Enzyme and pathway databases

BioCyciARA:AT4G16130-MONOMER.
MetaCyc:AT4G16130-MONOMER.

Miscellaneous databases

PROiO23461.

Gene expression databases

GenevisibleiO23461. AT.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.890. 2 hits.
InterProiIPR012369. Galk_glycosyltransferase.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10457. PTHR10457. 2 hits.
PfamiPF10509. GalKase_gal_bdg. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFiPIRSF036399. Gal_kin_glcsltr. 1 hit.
PRINTSiPR00959. MEVGALKINASE.
SUPFAMiSSF54211. SSF54211. 2 hits.
SSF55060. SSF55060. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
    Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.
    , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
    Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of a 14-kb fragment containing a putative cell wall gene and a candidate for the ARA1, arabinose kinase, gene from chromosome IV of Arabidopsis thaliana."
    Gy I., Aubourg S., Sherson S., Cobbett C.S., Cheron A., Kreis M., Lecharny A.
    Gene 209:201-210(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-1039.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 155-1039.
    Strain: cv. Columbia.
  7. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 453-1039.
    Strain: cv. Columbia.
  8. "The arabinose kinase, ARA1, gene of Arabidopsis is a novel member of the galactose kinase gene family."
    Sherson S., Gy I., Medd J., Schmidt R., Dean C., Kreis M., Lecharny A., Cobbett C.
    Plant Mol. Biol. 39:1003-1012(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLU-705.

Entry informationi

Entry nameiARAK_ARATH
AccessioniPrimary (citable) accession number: O23461
Secondary accession number(s): B9DI14, O23723, Q0WLW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Sup1, a suppressor of the ara1-1 arabinose-sensitive phenotype, contains a second point mutation resulting in a premature stop codon and a complete loss of kinase activity. Therefore, the putative arabinose transport function is independent from the kinase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.