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Protein

L-arabinokinase

Gene

ARA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Arabinose kinase. Involved in the salvage pathway which converts free L-arabinose to UDP-L-arabinose. May play a role in arabinose transport.1 Publication

Miscellaneous

Sup1, a suppressor of the ara1-1 arabinose-sensitive phenotype, contains a second point mutation resulting in a premature stop codon and a complete loss of kinase activity. Therefore, the putative arabinose transport function is independent from the kinase activity.

Catalytic activityi

ATP + L-arabinose = ADP + beta-L-arabinose 1-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei557Transition state stabilizerBy similarity1
Active sitei745Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi693 – 703ATPSequence analysisAdd BLAST11

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • galactose binding Source: InterPro
  • L-arabinokinase activity Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G16130-MONOMER
MetaCyc:AT4G16130-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
L-arabinokinase (EC:2.7.1.46)
Short name:
AtISA1
Gene namesi
Name:ARA1
Synonyms:ISA1
Ordered Locus Names:At4g16130
ORF Names:dl4105w, FCAALL.288
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT4G16130
TAIRilocus:2130105 AT4G16130

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei662 – 678HelicalSequence analysisAdd BLAST17

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi705E → K in ara1-1; arabinose sensitivity and loss of kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004074041 – 1039L-arabinokinaseAdd BLAST1039

Proteomic databases

PaxDbiO23461
PRIDEiO23461

PTM databases

iPTMnetiO23461

Expressioni

Gene expression databases

ExpressionAtlasiO23461 differential
GenevisibleiO23461 AT

Interactioni

Protein-protein interaction databases

BioGridi12593, 4 interactors
IntActiO23461, 3 interactors
STRINGi3702.AT4G16130.1

Structurei

3D structure databases

ProteinModelPortaliO23461
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi9 – 12Poly-Lys4
Compositional biasi21 – 24Poly-Leu4

Sequence similaritiesi

Belongs to the GHMP kinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0631 Eukaryota
COG0153 LUCA
HOGENOMiHOG000005811
InParanoidiO23461
KOiK12446
OMAiQHLVFAY
OrthoDBiEOG093600WY
PhylomeDBiO23461

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
InterProiView protein in InterPro
IPR012369 Galk_glycosyltransferase
IPR019539 GalKase_gal-bd
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF10509 GalKase_gal_bdg, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF036399 Gal_kin_glcsltr, 1 hit
SUPFAMiSSF54211 SSF54211, 2 hits
SSF55060 SSF55060, 3 hits

Sequencei

Sequence statusi: Complete.

O23461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERAKLQKK KKVTEDLLDS LLLLPPWRNF AISQSHQSII ESSSQRLPEK
60 70 80 90 100
MRIDENEGVS ASSKHLVFAY YVTGHGFGHA TRVVEVVRHL IAAGHDVHVV
110 120 130 140 150
TGAPDFVFTS EIQSPRLKIR KVLLDCGAVQ ADALTVDRLA SLEKYVETAV
160 170 180 190 200
VPRAEILETE VEWLHSIKAD FVVSDVVPVA CRAAADAGIR SVCVTNFSWD
210 220 230 240 250
FIYAEYVMAA GYHHRSIVWQ IAEDYSHCEF LIRLPGYCPM PAFRDVIDVP
260 270 280 290 300
LVVRRLHKSR KEVRKELGIA EDVNVVILNF GGQPSGWNLK ETSLPTGWLC
310 320 330 340 350
LVCGASETLE LPPNFIKLAK DAYTPDIIAA SDCMLGKIGY GTVSEALSYK
360 370 380 390 400
VPFVFVRRDY FNEEPFLRNM LEFYQCGVEM IRRDLLMGQW TPYLERAVSL
410 420 430 440 450
KPCYEGGING GEIAAHILQE TAIGRHCASD KLSGARRLRD AIILGYQLQR
460 470 480 490 500
VPGRDIAIPE WYSRAENELG QSAGSSPTVQ ANENNSLVES CIDDFDILQG
510 520 530 540 550
DVQGLSDTCT FLKSLAMLDA IHDSEKSTEK KTVRERKAAG GLFNWEEEIF
560 570 580 590 600
VARAPGRLDV MGGIADYSGS LVLQMPIREA CHVAVQRNLP GKHRLWKHAQ
610 620 630 640 650
ARQQAKGQVP TPVLQIVSYG SEISNRAPTF DMDLSDFMDG DEPISYEKAR
660 670 680 690 700
KFFAQDPAQK WAAYVAGTIL VLMIELGVRF EDSISLLVSS AVPEGKGVSS
710 720 730 740 750
SAAVEVASMS AIAAAHGLSI DPRDLAILCQ KVENHIVGAP CGVMDQMTSS
760 770 780 790 800
CGEANKLLAM ICQPAEVVGL VEIPNHVRFW GIDSGIRHSV GGADYRSVRV
810 820 830 840 850
GAYMGRKMIK SMASSILSPS ASSANGGNPE ELEDEGIDLL EAEASLDYLC
860 870 880 890 900
NLSPHRYEAR YADKLPDIML GQTFIEEYAD HDDPVTVIDQ KRSYSVKAPA
910 920 930 940 950
RHPIYENFRV KTFKALLTSA TSDEQLTALG GLLYQCHYSY SACGLGSDGT
960 970 980 990 1000
NRLVQLVQGM QHNKSNSEDG TLYGAKITGG GSGGTVCVVG RNSLRSSQQI
1010 1020 1030
LEIQQRYKAA TGYLPLIFEG SSPGAGKFGY LRIRRRISL
Length:1,039
Mass (Da):114,261
Last modified:January 1, 1998 - v1
Checksum:i82843127FCCB66B5
GO

Sequence cautioni

The sequence BT003908 differs from that shown. Reason: Frameshift at position 180.Curated
The sequence CAA74753 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144K → E in BT003908 (PubMed:14593172).Curated1
Sequence conflicti585V → A in CAA74753 (PubMed:9524266).Curated1
Sequence conflicti681E → G in BAH20381 (PubMed:19423640).Curated1
Sequence conflicti890Q → E in CAA74753 (PubMed:9524266).Curated1
Sequence conflicti961Q → R in BT003908 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97340 Genomic DNA Translation: CAB10392.1
AL161543 Genomic DNA Translation: CAB78655.1
CP002687 Genomic DNA Translation: AEE83696.1
BT003908 mRNA No translation available.
Y14404 Genomic DNA Translation: CAA74753.1 Different initiation.
AK317724 mRNA Translation: BAH20381.1
AK230073 mRNA Translation: BAF01893.1
PIRiF71427
RefSeqiNP_193348.1, NM_117706.3
UniGeneiAt.218
At.67863

Genome annotation databases

EnsemblPlantsiAT4G16130.1; AT4G16130.1; AT4G16130
GeneIDi827299
GrameneiAT4G16130.1; AT4G16130.1; AT4G16130
KEGGiath:AT4G16130

Similar proteinsi

Entry informationi

Entry nameiARAK_ARATH
AccessioniPrimary (citable) accession number: O23461
Secondary accession number(s): B9DI14, O23723, Q0WLW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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