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Reviewed, UniProtKB/Swiss-Prot O23320 (CML8_ARATH)

Last modified June 16, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Calmodulin-like protein 8
      Short name=AtCaM-8
Gene names
Name: CML8
Synonyms: CAM8
Ordered Locus Names: At4g14640
ORF Names: dl3360w, FCAALL.157
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length151 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Potential calcium sensor.

Tissue specificity

Expressed at low levels in leaves, flowers and siliques. Ref.1

Sequence similarities

Belongs to the calmodulin family.

Contains 4 EF-hand domains.

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Ontologies

Keywords
   DomainRepeat
   LigandCalcium
   PTMMethylation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcalcium-mediated signaling Ref.1

Inferred from genetic interaction. Source: TAIR

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

O221791EBI-1237764,EBI-1238013
P274501EBI-1237764,EBI-1238108
Q2V3591EBI-1237764,EBI-1235819
Q3EAG31EBI-1237764,EBI-1238312
Q3EBV71EBI-1237764,EBI-1235683
Q7X9N21EBI-1237764,EBI-622264
Q84MD81EBI-1237764,EBI-1238293
Q8H1D61EBI-1237764,EBI-1238166
Q8LPR41EBI-1237764,EBI-1238087
Q8VZ701EBI-1237764,EBI-1238078
Q93Z301EBI-1237764,EBI-1237782
Q9FGX21EBI-1237764,EBI-942623
Q9FGZ51EBI-1237764,EBI-622281
Q9FIW61EBI-1237764,EBI-1237967
Q9FKR21EBI-1237764,EBI-622203
Q9FM431EBI-1237764,EBI-1238097
Q9LW661EBI-1237764,EBI-1238066
Q9LX561EBI-1237764,EBI-1235891
Q9SGN71EBI-1237764,EBI-1238190
Q9SJX81EBI-1237764,EBI-1238001
Q9SKN21EBI-1237764,EBI-1237944
ABF4Q9M7Q21EBI-1237764,EBI-1237867
AGL3P293831EBI-1237764,EBI-622036
AGL61Q4PSU41EBI-1237764,EBI-1237976
AGL8Q388761EBI-1237764,EBI-621912
AGL80Q9FJK31EBI-1237764,EBI-622424
ALDH3F1Q70E961EBI-1237764,EBI-1237774
ASK6Q390101EBI-1237764,EBI-1238323
At1g08320Q56W431EBI-1237764,EBI-1237844
At1g20950/F9H16.6Q9SYP21EBI-1237764,EBI-1238145
At1g54610Q9ZVM91EBI-1237764,EBI-1235713
At1g58110Q84WC81EBI-1237764,EBI-1237819
At1g60920Q9C9601EBI-1237764,EBI-622255
At1g77145O806511EBI-1237764,EBI-1238133
At3g05050Q9MAB41EBI-1237764,EBI-1237895
AT3G15010Q9LKA41EBI-1237764,EBI-1238304
At3g17600Q2VW981EBI-1237764,EBI-1235997
AT4g02410O812921EBI-1237764,EBI-1237950
AT4g02640O227631EBI-1237764,EBI-969959
AT4g34380Q9SZ031EBI-1237764,EBI-1238343
At4g37240/C7A10_120Q8LE561EBI-1237764,EBI-1237996
At4g39540/F23K16_170Q8GY881EBI-1237764,EBI-1238334
At5g38800Q9FMC21EBI-1237764,EBI-1237855
At5g49760/K2I5_13Q8GZ991EBI-1237764,EBI-1238218
BIK1O488141EBI-1237764,EBI-1238176
BZIP61Q9M2K41EBI-1237764,EBI-1237830
CDKB2-2Q8LG641EBI-1237764,EBI-1235761
CIPK24Q9LDI31EBI-1237764,EBI-537551
CKI1Q390501EBI-1237764,EBI-1237881
CML45Q93Z271EBI-1237764,EBI-1235938
CPK3Q424791EBI-1237764,EBI-1235782
CPK30Q9SSF81EBI-1237764,EBI-1235738
CRK40Q9SYS31EBI-1237764,EBI-1238119
CRK9O654691EBI-1237764,EBI-1238158
CYP705A20Q9LJY71EBI-1237764,EBI-1237907
dl4570wO235521EBI-1237764,EBI-1235805
F11C1_150Q9SND61EBI-1237764,EBI-1235872
F14F8_210Q9LFU31EBI-1237764,EBI-1237805
F18O21_230Q9LYL61EBI-1237764,EBI-1238139
F23H24.1Q9SYB91EBI-1237764,EBI-1238042
FBW2Q9ZPE41EBI-1237764,EBI-604740
FBX14Q8RWQ81EBI-1237764,EBI-1235922
HSP81-2P557371EBI-1237764,EBI-1235834
LPD2Q9M5K21EBI-1237764,EBI-1237919
LRR-RLKQ3ECN41EBI-1237764,EBI-1238268
LRR-RLKQ8VYG71EBI-1237764,EBI-1238236
LRR-RLKQ9LSI91EBI-1237764,EBI-1238253
MHKP432941EBI-1237764,EBI-1238022
NEK7Q9LHI71EBI-1237764,EBI-1238055
RKF3P930501EBI-1237764,EBI-1238281
SHP1Q5XXJ31EBI-1237764,EBI-1237985
T20L15_160Q9LZV71EBI-1237764,EBI-1238200
T22D6_80Q9LEZ21EBI-1237764,EBI-1237792
T4M14.10Q9LQ651EBI-1237764,EBI-942986
WAKL21Q8GYF51EBI-1237764,EBI-1238355
WRKY43Q8GY111EBI-1237764,EBI-1235953
WRKY53Q9SUP61EBI-1237764,EBI-1235980

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 151151Calmodulin-like protein 8
PRO_0000073652

Regions

Domain9 – 4436EF-hand 1
Domain45 – 8036EF-hand 2
Domain82 – 11736EF-hand 3
Domain118 – 15134EF-hand 4
Calcium binding22 – 33121 Potential
Calcium binding58 – 69122 Potential
Calcium binding95 – 106123 Potential
Calcium binding131 – 142124 Potential

Amino acid modifications

Modified residue1171N6,N6,N6-trimethyllysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
O23320-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A72571DC7EAB2427

FASTA15117,156
        10         20         30         40         50         60 
MEETALTKDQ ITEFKEAFCL FDKDGDGCIT VEELATVIRS LDQNPTEQEL HDIITEIDSD 

        70         80         90        100        110        120 
SNGTIEFAEF LNLMAKKLQE SDAEEELKEA FKVFDKDQNG YISASELSHV MINLGEKLTD 

       130        140        150 
EEVEQMIKEA DLDGDGQVNY DEFVKMMINI D

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of three new members of the Arabidopsis thaliana calmodulin gene family: conserved and highly diverged members of the gene family functionally complement a yeast calmodulin null."
Zielinski R.E.
Planta 214:446-455(2002) [PubMed: 11855649] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana."
Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. expand/collapse author list , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.
Nature 391:485-488(1998) [PubMed: 9461215] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Kim C.J., Chen H., Shinn P., Ecker J.R.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Calmodulins and related potential calcium sensors of Arabidopsis."
McCormack E., Braam J.
New Phytol. 159:585-598(2003)
Cited for: GENE FAMILY, NOMENCLATURE.

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Cross-references

Sequence databases

AF178074 mRNA. Translation: AAD53314.1.
Z97336 Genomic DNA. Translation: CAB10243.1.
AL161539 Genomic DNA. Translation: CAB78506.1.
BT024688 mRNA. Translation: ABD57513.1.
IPIIPI00528531.
PIRA71409.
RefSeqNP_193200.1.
UniGeneAt.4288

3D structure databases

HSSPHSSP built from PDB template 1A29 based on UniProtKB P62157.
SMRO23320. Positions 6-148.
ModBaseSearch...

Protein-protein interaction databases

IntActO23320. 77 interactions.

Genome annotation databases

GeneID827114.
GenomeReviewsGene locus AT4G14640 in contig CT486007_GR.
KEGGath:AT4G14640.

Organism-specific databases

GeneFarm5185.
TAIRAt4g14640.

Phylogenomic databases

OMAO23320. EQMIKEA.

Gene expression databases

ArrayExpressO23320.
GermOnlineAT4G14640. Arabidopsis thaliana.

Family and domain databases

InterProIPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca_bd.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PfamPF00036. efhand. 4 hits.
[Graphical view]
ProDomPD000012. EF-hand. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00054. EFh. 4 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCML8_ARATH
AccessionPrimary (citable) accession number: O23320
Secondary accession number(s): Q29Q68
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents