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Protein

DNA (cytosine-5)-methyltransferase 4

Gene

MET4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. Required for proper patterns of CG DNA methylation in dividing cells (By similarity).By similarity

Caution

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1183PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • DNA mediated transformation Source: TAIR

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-ATH-212300 PRC2 methylates histones and DNA

Protein family/group databases

REBASEi2839 M.AthMET2

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 4 (EC:2.1.1.37)
Alternative name(s):
DNA methyltransferase 4
DNA methyltransferase IIa
Short name:
DMT02
Short name:
MET02
Gene namesi
Name:MET4
Synonyms:DMT2, MET2, METIIa
Ordered Locus Names:At4g14140
ORF Names:dl3110w
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G14140
TAIRilocus:2129450 AT4G14140

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004300131 – 1519DNA (cytosine-5)-methyltransferase 4Add BLAST1519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki583Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO23273

PTM databases

iPTMnetiO23273

Expressioni

Tissue specificityi

Expressed at low levels in vegetative and floral organs.1 Publication

Gene expression databases

ExpressionAtlasiO23273 baseline and differential
GenevisibleiO23273 AT

Interactioni

Protein-protein interaction databases

BioGridi123491 interactor.
STRINGi3702.AT4G14140.2

Structurei

3D structure databases

ProteinModelPortaliO23273
SMRiO23273
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini715 – 849BAH 1PROSITE-ProRule annotationAdd BLAST135
Domaini916 – 1033BAH 2PROSITE-ProRule annotationAdd BLAST118
Domaini1078 – 1512SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST435

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi632 – 671Glu-richAdd BLAST40

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF68 Eukaryota
COG0270 LUCA
HOGENOMiHOG000083447
KOiK00558
OMAiGHETYKA
OrthoDBiEOG09360085
PhylomeDBiO23273

Family and domain databases

InterProiView protein in InterPro
IPR001025 BAH_dom
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR022702 Cytosine_MeTrfase1_RFD
IPR017198 DNMT1-like
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01426 BAH, 2 hits
PF00145 DNA_methylase, 2 hits
PF12047 DNMT1-RFD, 2 hits
PIRSFiPIRSF037404 DNMT1, 1 hit
PRINTSiPR00105 C5METTRFRASE
SMARTiView protein in SMART
SM00439 BAH, 2 hits
SUPFAMiSSF53335 SSF53335, 3 hits
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS51038 BAH, 2 hits
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O23273-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMETKAGKQ KKRSVDSDDD VSKERRPKRA AACTNFKEKS LRISDKSETV
60 70 80 90 100
EAKKEQILAE EIVAIQLTSS LESNDDPRPN RRLTDFVLHD SEGVPQPVEM
110 120 130 140 150
LELGDIFIEG VVLPLGDEKK EEKGVRFQSF GRVENWNISG YEDGSPVIWI
160 170 180 190 200
STALADYDCR KPSKKYKKLY DYFFEKACAC VEVFKSLSKN PDTSLDELLA
210 220 230 240 250
AVSRSMSGSK IFSSGGAIQE FVISQGEFIY NQLAGLDETA KNHETCFVEN
260 270 280 290 300
RVLVSLRDHE SNKIHKALSN VALRIDESKV VTSDHLVDGA EDEDVKYAKL
310 320 330 340 350
IQEEEYRKSM ERSRNKRSST TSGGSSRFYI KISEDEIADD YPLPSYYKNT
360 370 380 390 400
KEETDELVLF EAGYEVDTRD LPCRTLHNWT LYNSDSRMIS LEVLPMRPCA
410 420 430 440 450
EIDVTVFGSG VVAEDDGSGF CLDDSESSTS TQSNDHDGMN IFLSQIKEWM
460 470 480 490 500
IEFGAEMIFV TLRTDMAWYR LGKPSKQYAP WFGTVMKTVR VGISIFNMLM
510 520 530 540 550
RESRVAKLSY ANVIKRLCGL EENDKAYISS KLLDVERYVV VHGQIILQLF
560 570 580 590 600
EEYPDKDIKR CPFVTSLASK MQDIHHTKWI IKKKKKILQK GKNLNPRAGI
610 620 630 640 650
APVVSRMKAM QATTTRLVNR IWGEFYSIYS PEVPSEAINA ENVEEEELEE
660 670 680 690 700
VEEEDENEED DPEENELEAV EIQNSPTPKK IKGISEDMEI KWDGEILGKT
710 720 730 740 750
SAGEPLYGRA FVGGDVVVVG SAVILEVDDQ DDTQLICFVE FMFESSNHSK
760 770 780 790 800
MLHGKLLQRG SETVLGMAAN ERELFLTNEC LTVQLKDIKG TVSLEIRSRL
810 820 830 840 850
WGHQYRKENI DVDKLDRARA EERKTNGLPT DYYCKSLYSP ERGGFFSLPR
860 870 880 890 900
NDMGLGSGFC SSCKIRENEE ERSKTKLNDS KTGFLSNGIE YHNGDFVYVL
910 920 930 940 950
PNYITKDGLK KGSRRTTLKC GRNVGLKAFV VCQLLDVIVL EESRKASKAS
960 970 980 990 1000
FQVKLTRFYR PEDISEEKAY ASDIQELYYS QDTYILPPEA IQGKCEVRKK
1010 1020 1030 1040 1050
SDMPLCREYP ILDHIFFCEV FYDSSTGYLK QFPANMKLKF STIKDETLLR
1060 1070 1080 1090 1100
EKKGKGVETG TSSGMLMKPD EVPKEKPLAT LDIFAGCGGL SHGLENAGVS
1110 1120 1130 1140 1150
TTKWAIEYEE PAGHAFKQNH PEATVFVDNC NVILRAIMEK CGDVDDCVST
1160 1170 1180 1190 1200
VEAAELAAKL DENQKSTLPL PGQVDFINGG PPCQGFSGMN RFSHGSWSKV
1210 1220 1230 1240 1250
QCEMILAFLS FADYFRPKYF LLENVKKFVT YNKGRTFQLT MASLLEMGYQ
1260 1270 1280 1290 1300
VRFGILEAGT YGVSQPRKRV IIWAASPEEV LPEWPEPMHV FDNPGSKISL
1310 1320 1330 1340 1350
PRGLRYDAGC NTKFGAPFRS ITVRDTIGDL PPVENGESKI NKEYGTTPAS
1360 1370 1380 1390 1400
WFQKKIRGNM SVLTDHICKG LNELNLIRCK KIPKRPGADW RDLPDENVTL
1410 1420 1430 1440 1450
SNGLVEKLRP LALSKTAKNH NEWKGLYGRL DWQGNLPISI TDPQPMGKVG
1460 1470 1480 1490 1500
MCFHPEQDRI ITVRECARSQ GFPDSYEFSG TTKHKHRQIG NAVPPPLAFA
1510
LGRKLKEALY LKSSLQHQS
Length:1,519
Mass (Da):171,586
Last modified:January 1, 1998 - v1
Checksum:i8BD760A15FA90DA4
GO
Isoform 2 (identifier: O23273-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1099-1135: VSTTKWAIEY...FVDNCNVILR → MYLYSHVMHI...KQRFLLTTAM

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:1,545
Mass (Da):174,825
Checksum:iEF55DAD9992D7A1F
GO

Sequence cautioni

The sequence AAF14882 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554011099 – 1135VSTTK…NVILR → MYLYSHVMHILLSSKHLKTF IKMHVLCNKVYLLQSGRSSM KSQLVMRLNKTILKQRFLLT TAM in isoform 2. CuratedAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF138283 Genomic DNA Translation: AAF14882.1 Different initiation.
Z97335 Genomic DNA Translation: CAB10193.1
AL161538 Genomic DNA Translation: CAB78456.1
CP002687 Genomic DNA Translation: AEE83379.1
CP002687 Genomic DNA Translation: AEE83380.1
CP002687 Genomic DNA Translation: ANM66066.1
PIRiG71402
RefSeqiNP_001190725.1, NM_001203796.1 [O23273-2]
NP_001319931.1, NM_001340902.1 [O23273-1]
NP_193150.1, NM_117491.1 [O23273-1]
UniGeneiAt.51020

Genome annotation databases

EnsemblPlantsiAT4G14140.1; AT4G14140.1; AT4G14140 [O23273-1]
AT4G14140.2; AT4G14140.2; AT4G14140 [O23273-2]
AT4G14140.3; AT4G14140.3; AT4G14140 [O23273-1]
GeneIDi827052
GrameneiAT4G14140.1; AT4G14140.1; AT4G14140 [O23273-1]
AT4G14140.2; AT4G14140.2; AT4G14140 [O23273-2]
AT4G14140.3; AT4G14140.3; AT4G14140 [O23273-1]
KEGGiath:AT4G14140

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNMT4_ARATH
AccessioniPrimary (citable) accession number: O23273
Secondary accession number(s): F4JUL5, Q9SEG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome