Reviewed,
UniProtKB/Swiss-Prot O23255 (SAHH1_ARATH)
Last modified
February 9, 2010.
Version 88.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Adenosylhomocysteinase 1 Short name=AdoHcyase 1 EC=3.3.1.1 Alternative name(s): S-adenosyl-L-homocysteine hydrolase 1 Short name=SAH hydrolase 1 Protein HOMOLOGY-DEPENDENT GENE SILENCING 1 Protein EMBRYO DEFECTIVE 1395 | ||||||||
| Gene names |
| ||||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 485 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methinine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Required for DNA methylation-dependent gene silencing. |
| Catalytic activity | S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. |
| Cofactor | Binds 1 NAD per subunit. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Miscellaneous | Null mutations are homozygous lethal. |
| Sequence similarities | Belongs to the adenosylhomocysteinase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | One-carbon metabolism |
| Coding sequence diversity | Alternative splicing |
| Ligand | NAD |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | embryonic development ending in seed dormancy Inferred from mutant phenotype. Source: TAIR methylation-dependent chromatin silencing Ref.2Inferred from mutant phenotype. Source: TAIR one-carbon metabolic processInferred from electronic annotation. Source: UniProtKB-KW posttranscriptional gene silencingInferred from mutant phenotype. Source: TAIR |
| Cellular component | plasma membrane Inferred from direct assay. Source: TAIR vacuoleInferred from direct assay. Source: TAIR |
| Molecular function | adenosylhomocysteinase activity Ref.2 Inferred from mutant phenotype. Source: TAIR copper ion bindingInferred from direct assay. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: O23255-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 485 | 485 | Adenosylhomocysteinase 1 | PRO_0000116920 | |||||
Regions | |||||||||
| Region | 232 – 401 | 170 | NAD binding By similarity | ||||||
Sites | |||||||||
| Binding site | 64 | 1 | Substrate By similarity | ||||||
| Binding site | 139 | 1 | Substrate By similarity | ||||||
| Binding site | 205 | 1 | Substrate By similarity | ||||||
| Binding site | 235 | 1 | Substrate By similarity | ||||||
| Binding site | 239 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 386 | 1 | G → E in hog1-2; genome-wide demethylation and loss of transcriptional gene silencing. Ref.2 | ||||||
| Mutagenesis | 414 | 1 | T → I in hog1-1; genome-wide demethylation and loss of transcriptional gene silencing. Ref.2 | ||||||
| Mutagenesis | 444 | 1 | D → N in hog1-3; genome-wide demethylation and loss of transcriptional gene silencing. Ref.2 | ||||||
| Sequence conflict | 80 | 1 | E → Q in CAB09795. Ref.7 | ||||||
| Sequence conflict | 96 | 1 | A → R in CAB09795. Ref.7 | ||||||
| Sequence conflict | 392 | 1 | E → Q in CAB09795. Ref.7 | ||||||
| Sequence conflict | 460 | 1 | T → R in CAB09795. Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The isolation of an Arabidopsis thaliana cDNA clone encoding S-adenosyl-L-homocysteine hydrolase." Belbahri L., Elleuch H., Villarroel R., Inze D., Thomas D., Thomasset B. Plant Gene Register PGR99-139 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta. |
| [2] | "The Arabidopsis HOMOLOGY-DEPENDENT GENE SILENCING1 gene codes for an S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing." Rocha P.S., Sheikh M., Melchiorre R., Fagard M., Boutet S., Loach R., Moffatt B., Wagner C., Vaucheret H., Furner I. Plant Cell 17:404-417(2005) [PubMed: 15659630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLY-386; THR-414 AND ASP-444. |
| [3] | "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. Chalwatzis N.Nature 391:485-488(1998) [PubMed: 9461215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [5] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [6] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [7] | "Identification of novel nitrate-inducible genes from Arabidopsis." Zhang H., Forde B.G. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 19-485. Strain: cv. Landsberg erecta. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z97335 Genomic DNA. Translation: CAB10173.1. AF059581 mRNA. Translation: AAC14714.1. AL161537 Genomic DNA. Translation: CAB78436.1. Z97059 mRNA. Translation: CAB09795.1. AF325037 mRNA. Translation: AAG40389.1. AY042866 mRNA. Translation: AAK68806.1. AY049279 mRNA. Translation: AAK83621.1. AY081468 mRNA. Translation: AAM10030.1. AY090284 mRNA. Translation: AAL90945.1. AY085669 mRNA. Translation: AAM62888.1. BT002404 mRNA. Translation: AAO00764.1. |
| IPI | IPI00527713. |
| PIR | C71400. |
| RefSeq | NP_193130.1. |
| UniGene | At.24056 At.24845 At.71469 At.71956 Rra.8659 Rsa.8091 |
3D structure databases | |
| SMR | O23255. Positions 12-485. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O23255. |
Proteomic databases | |
| PRIDE | O23255. |
| ProMEX | O23255. |
Genome annotation databases | |
| GeneID | 827028. |
| GenomeReviews | Gene locus AT4G13940 in contig CT486007_GR. |
| NMPDR | fig|3702.1.peg.19089. |
Organism-specific databases | |
| TAIR | At4g13940. |
Phylogenomic databases | |
| HOGENOM | HBG352029. |
| InParanoid | O23255. |
| OMA | VITHDHM. |
| PhylomeDB | O23255. |
Enzyme and pathway databases | |
| BRENDA | 3.3.1.1. 302. |
Gene expression databases | |
| ArrayExpress | O23255. |
| Genevestigator | O23255. |
Family and domain databases | |
| InterPro | IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view] |
| PANTHER | PTHR23420. Ad_hcy_hydrolase. 1 hit. |
| Pfam | PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view] |
| PIRSF | PIRSF001109. Ad_hcy_hydrolase. 1 hit. |
| TIGRFAMs | TIGR00936. ahcY. 1 hit. |
| PROSITE | PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SAHH1_ARATH | ||||||||
| Accession | Primary (citable) accession number: O23255 Secondary accession number(s): O81847, Q8LE20 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


