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Protein

Adenosylhomocysteinase 1

Gene

SAHH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Required for DNA methylation-dependent gene silencing.

Miscellaneous

Null mutations are homozygous lethal.

Catalytic activityi

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: L-homocysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase, Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase 2 (SAHH2), Adenosylhomocysteinase 1 (SAHH1), Adenosylhomocysteinase (HOG1), Adenosylhomocysteinase (AXX17_At4g15900), Adenosylhomocysteinase (AXX17_At3g25710), Adenosylhomocysteinase (HOG1)
This subpathway is part of the pathway L-homocysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-homocysteine from S-adenosyl-L-homocysteine, the pathway L-homocysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64SubstrateBy similarity1
Binding sitei139SubstrateBy similarity1
Binding sitei205SubstrateBy similarity1
Binding sitei235SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei240NADBy similarity1
Binding sitei292NADBy similarity1
Binding sitei327NADBy similarity1
Binding sitei397NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 208NADBy similarity3
Nucleotide bindingi269 – 274NADBy similarity6
Nucleotide bindingi348 – 350NADBy similarity3

GO - Molecular functioni

  • adenosylhomocysteinase activity Source: TAIR
  • copper ion binding Source: TAIR
  • NAD binding Source: InterPro

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • methylation-dependent chromatin silencing Source: TAIR
  • one-carbon metabolic process Source: UniProtKB-KW
  • posttranscriptional gene silencing Source: TAIR
  • S-adenosylhomocysteine catabolic process Source: InterPro
  • S-adenosylmethionine cycle Source: GO_Central

Keywordsi

Molecular functionHydrolase
Biological processOne-carbon metabolism
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT4G13940-MONOMER.
ReactomeiR-ATH-156581. Methylation.
R-ATH-1614635. Sulfur amino acid metabolism.
UniPathwayiUPA00314; UER00076.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylhomocysteinase 1 (EC:3.3.1.1)
Short name:
AdoHcyase 1
Alternative name(s):
Protein EMBRYO DEFECTIVE 1395
Protein HOMOLOGY-DEPENDENT GENE SILENCING 1
S-adenosyl-L-homocysteine hydrolase 1
Short name:
SAH hydrolase 1
Gene namesi
Name:SAHH1
Synonyms:EMB1395, HOG1
Ordered Locus Names:At4g13940
ORF Names:dl3010w
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G13940.
TAIRilocus:2129256. AT4G13940.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi386G → E in hog1-2; genome-wide demethylation and loss of transcriptional gene silencing. 1 Publication1
Mutagenesisi414T → I in hog1-1; genome-wide demethylation and loss of transcriptional gene silencing. 1 Publication1
Mutagenesisi444D → N in hog1-3; genome-wide demethylation and loss of transcriptional gene silencing. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001169201 – 485Adenosylhomocysteinase 1Add BLAST485

Proteomic databases

PaxDbiO23255.
PRIDEiO23255.

PTM databases

iPTMnetiO23255.

Expressioni

Gene expression databases

ExpressionAtlasiO23255. baseline and differential.
GenevisibleiO23255. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi12325. 7 interactors.
IntActiO23255. 2 interactors.
STRINGi3702.AT4G13940.1.

Structurei

3D structure databases

ProteinModelPortaliO23255.
SMRiO23255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosylhomocysteinase family.Curated

Phylogenomic databases

eggNOGiKOG1370. Eukaryota.
COG0499. LUCA.
HOGENOMiHOG000227986.
InParanoidiO23255.
KOiK01251.
OMAiFWHKAVA.
OrthoDBiEOG093607MR.
PhylomeDBiO23255.

Family and domain databases

CDDicd00401. SAHH. 1 hit.
HAMAPiMF_00563. AdoHcyase. 1 hit.
InterProiView protein in InterPro
IPR034373. Adenosylhomocysteinase.
IPR000043. Adenosylhomocysteinase-like.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR020082. S-Ado-L-homoCys_hydrolase_CS.
PANTHERiPTHR23420. PTHR23420. 1 hit.
PfamiView protein in Pfam
PF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
PIRSFiPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTiView protein in SMART
SM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00936. ahcY. 1 hit.
PROSITEiView protein in PROSITE
PS00738. ADOHCYASE_1. 1 hit.
PS00739. ADOHCYASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O23255-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALLVEKTSS GREYKVKDMS QADFGRLELE LAEVEMPGLM ACRTEFGPSQ
60 70 80 90 100
PFKGARITGS LHMTIQTAVL IETLTALGAE VRWCSCNIFS TQDHAAAAIA
110 120 130 140 150
RDSAAVFAWK GETLQEYWWC TERALDWGPG GGPDLIVDDG GDATLLIHEG
160 170 180 190 200
VKAEEIFEKT GQVPDPTSTD NPEFQIVLSI IKEGLQVDPK KYHKMKERLV
210 220 230 240 250
GVSEETTTGV KRLYQMQQNG TLLFPAINVN DSVTKSKFDN LYGCRHSLPD
260 270 280 290 300
GLMRATDVMI AGKVAVICGY GDVGKGCAAA MKTAGARVIV TEIDPICALQ
310 320 330 340 350
ALMEGLQVLT LEDVVSEADI FVTTTGNKDI IMVDHMRKMK NNAIVCNIGH
360 370 380 390 400
FDNEIDMLGL ETYPGVKRIT IKPQTDRWVF PETKAGIIVL AEGRLMNLGC
410 420 430 440 450
ATGHPSFVMS CSFTNQVIAQ LELWNEKASG KYEKKVYVLP KHLDEKVALL
460 470 480
HLGKLGARLT KLSKDQSDYV SIPIEGPYKP PHYRY
Length:485
Mass (Da):53,379
Last modified:January 1, 1998 - v1
Checksum:i1113270A0F46C86C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti80E → Q in CAB09795 (Ref. 8) Curated1
Sequence conflicti96A → R in CAB09795 (Ref. 8) Curated1
Sequence conflicti392E → Q in CAB09795 (Ref. 8) Curated1
Sequence conflicti460T → R in CAB09795 (Ref. 8) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059581 mRNA. Translation: AAC14714.1.
Z97335 Genomic DNA. Translation: CAB10173.1.
AL161537 Genomic DNA. Translation: CAB78436.1.
CP002687 Genomic DNA. Translation: AEE83345.1.
AF325037 mRNA. Translation: AAG40389.1.
AY042866 mRNA. Translation: AAK68806.1.
AY049279 mRNA. Translation: AAK83621.1.
AY081468 mRNA. Translation: AAM10030.1.
AY090284 mRNA. Translation: AAL90945.1.
BT002404 mRNA. Translation: AAO00764.1.
AY085669 mRNA. Translation: AAM62888.1.
Z97059 mRNA. Translation: CAB09795.1.
PIRiC71400.
RefSeqiNP_193130.1. NM_117468.3. [O23255-1]
UniGeneiAt.24056.
At.24845.
At.71956.
At.74987.
At.74988.

Genome annotation databases

EnsemblPlantsiAT4G13940.1; AT4G13940.1; AT4G13940. [O23255-1]
GeneIDi827028.
GrameneiAT4G13940.1; AT4G13940.1; AT4G13940.
KEGGiath:AT4G13940.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSAHH1_ARATH
AccessioniPrimary (citable) accession number: O23255
Secondary accession number(s): O81847, Q8LE20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: October 25, 2017
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families