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Protein

Eukaryotic translation initiation factor 4E-1

Gene

EIF4E1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • regulation of translation Source: UniProtKB-KW
  • response to virus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E-1
Short name:
eIF-4E-1
Short name:
eIF4E-1
Alternative name(s):
Protein cucumovirus multiplication 1
eIF-4F 25 kDa subunit
eIF-4F p26 subunit
mRNA cap-binding protein
Gene namesi
Name:EIF4E1
Synonyms:CUM1
Ordered Locus Names:At4g18040
ORF Names:F15J5.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G18040.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytoplasmic mRNA processing body Source: TAIR
  • cytoplasmic stress granule Source: TAIR
  • mRNA cap binding complex Source: UniProtKB
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Eukaryotic translation initiation factor 4E-1PRO_0000193654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi133 ↔ 171By similarity

Post-translational modificationi

According to the redox status, the Cys-133-Cys-171 disulfide bridge may have a role in regulating protein function by affecting its ability to bind capped mRNA.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO23252.
PRIDEiO23252.

PTM databases

iPTMnetiO23252.

Expressioni

Tissue specificityi

Expressed in all tissues except in the cells of the specialization zone of the roots.1 Publication

Gene expression databases

ExpressionAtlasiO23252. baseline and differential.
GenevisibleiO23252. AT.

Interactioni

Subunit structurei

EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G. EIF4E is also known to interact with other partners. In higher plants two isoforms of EIF4F have been identified, named isoform EIF4F and isoform EIF(iso)4F. Isoform EIF4F has subunits p220 and p26, whereas isoform EIF(iso)4F has subunits p82 and p28.1 Publication

Protein-protein interaction databases

BioGridi12822. 2 interactions.
IntActiO23252. 1 interaction.
STRINGi3702.AT4G18040.1.

Structurei

3D structure databases

ProteinModelPortaliO23252.
SMRiO23252. Positions 60-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 8327-methylguanosine-containing mRNA cap bindingBy similarity
Regioni128 – 12927-methylguanosine-containing mRNA cap bindingBy similarity
Regioni178 – 18367-methylguanosine-containing mRNA cap bindingBy similarity
Regioni223 – 22537-methylguanosine-containing mRNA cap bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1670. Eukaryota.
COG5053. LUCA.
HOGENOMiHOG000186751.
InParanoidiO23252.
KOiK03259.
OMAiLDYNNSI.
OrthoDBiEOG09360N1A.
PhylomeDBiO23252.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEDTPKSV VTEEAKPNSI ENPIDRYHEE GDDAEEGEIA GGEGDGNVDE
60 70 80 90 100
SSKSGVPESH PLEHSWTFWF DNPAVKSKQT SWGSSLRPVF TFSTVEEFWS
110 120 130 140 150
LYNNMKHPSK LAHGADFYCF KHIIEPKWED PICANGGKWT MTFPKEKSDK
160 170 180 190 200
SWLYTLLALI GEQFDHGDEI CGAVVNIRGK QERISIWTKN ASNEAAQVSI
210 220 230
GKQWKEFLDY NNSIGFIIHE DAKKLDRNAK NAYTA
Length:235
Mass (Da):26,519
Last modified:January 1, 1998 - v1
Checksum:i0329A375FE19A3C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10548 mRNA. Translation: CAA71580.1.
AL110123 Genomic DNA. Translation: CAB53645.1.
AL161547 Genomic DNA. Translation: CAB78806.1.
CP002687 Genomic DNA. Translation: AEE83987.1.
AF389286 mRNA. Translation: AAK63858.1.
AY093750 mRNA. Translation: AAM10374.1.
AY086496 mRNA. Translation: AAM63497.1.
PIRiT14804.
RefSeqiNP_193538.1. NM_117914.3.
UniGeneiAt.40.
At.67794.

Genome annotation databases

EnsemblPlantsiAT4G18040.1; AT4G18040.1; AT4G18040.
GeneIDi827529.
GrameneiAT4G18040.1; AT4G18040.1; AT4G18040.
KEGGiath:AT4G18040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10548 mRNA. Translation: CAA71580.1.
AL110123 Genomic DNA. Translation: CAB53645.1.
AL161547 Genomic DNA. Translation: CAB78806.1.
CP002687 Genomic DNA. Translation: AEE83987.1.
AF389286 mRNA. Translation: AAK63858.1.
AY093750 mRNA. Translation: AAM10374.1.
AY086496 mRNA. Translation: AAM63497.1.
PIRiT14804.
RefSeqiNP_193538.1. NM_117914.3.
UniGeneiAt.40.
At.67794.

3D structure databases

ProteinModelPortaliO23252.
SMRiO23252. Positions 60-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12822. 2 interactions.
IntActiO23252. 1 interaction.
STRINGi3702.AT4G18040.1.

PTM databases

iPTMnetiO23252.

Proteomic databases

PaxDbiO23252.
PRIDEiO23252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G18040.1; AT4G18040.1; AT4G18040.
GeneIDi827529.
GrameneiAT4G18040.1; AT4G18040.1; AT4G18040.
KEGGiath:AT4G18040.

Organism-specific databases

TAIRiAT4G18040.

Phylogenomic databases

eggNOGiKOG1670. Eukaryota.
COG5053. LUCA.
HOGENOMiHOG000186751.
InParanoidiO23252.
KOiK03259.
OMAiLDYNNSI.
OrthoDBiEOG09360N1A.
PhylomeDBiO23252.

Miscellaneous databases

PROiO23252.

Gene expression databases

ExpressionAtlasiO23252. baseline and differential.
GenevisibleiO23252. AT.

Family and domain databases

Gene3Di3.30.760.10. 1 hit.
InterProiIPR023398. TIF_eIF4e-like.
IPR001040. TIF_eIF_4E.
IPR019770. TIF_eIF_4E_CS.
[Graphical view]
PANTHERiPTHR11960. PTHR11960. 1 hit.
PfamiPF01652. IF4E. 1 hit.
[Graphical view]
SUPFAMiSSF55418. SSF55418. 1 hit.
PROSITEiPS00813. IF4E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4E1_ARATH
AccessioniPrimary (citable) accession number: O23252
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.