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Protein

Purple acid phosphatase 25

Gene

PAP25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi164IronBy similarity1
Metal bindingi192IronBy similarity1
Metal bindingi192ZincBy similarity1
Metal bindingi195IronBy similarity1
Metal bindingi229ZincBy similarity1
Binding sitei229SubstrateBy similarity1
Metal bindingi314ZincBy similarity1
Active sitei324Proton donorBy similarity1
Metal bindingi351ZincBy similarity1
Metal bindingi353IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: TAIR

GO - Biological processi

  • phosphate-containing compound metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G36350-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 25 (EC:3.1.3.2)
Gene namesi
Name:PAP25
Synonyms:AT7, ATH2
Ordered Locus Names:At4g36350
ORF Names:C7A10.1010, F23E13.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G36350.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000037282822 – 466Purple acid phosphatase 25Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Glycosylationi424N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO23244.
PRIDEiO23244.

PTM databases

iPTMnetiO23244.

Expressioni

Tissue specificityi

Specifically expressed in flowers.1 Publication

Gene expression databases

GenevisibleiO23244. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G36350.1.

Structurei

3D structure databases

ProteinModelPortaliO23244.
SMRiO23244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 353Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiO23244.
OMAiEASIDMP.
OrthoDBiEOG09360APJ.
PhylomeDBiO23244.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMNKILLVF VFLSIATVIN SGTTSNFVRT AQPSTEMSLE TFPSPAGHNA
60 70 80 90 100
PEQVHIVQGD YNGRGIIISW VTPLNLAGSN VVTYWKAVDG DVKPKKKRGH
110 120 130 140 150
ASTSSYRFYD YTSGFLHHAT IKGLEYDTKY IYEVGTDGSV RQFSFTSPPK
160 170 180 190 200
VGPDVPYTFG IIGDLGQTLA SNETLYHYMS NPKGQAVLFP GDLSYADDHP
210 220 230 240 250
NHDQRKWDSW GRFVEPCAAY QTFIYAAGNH EIDFVPNIGE PHAFKPYIHR
260 270 280 290 300
YHNAYKASKS ISPLWYSIRR ASAHIIVLSS YSAYGKYTPQ YVWLEQELKK
310 320 330 340 350
VNREETPWLI VMVHSPWYNS NNYHYMEGES MRAMFESWFV NSKVDLVLSG
360 370 380 390 400
HVHSYERSER VSNIKYNITN GLSYPVKDPS APIYITIGDG GNIEGIANSF
410 420 430 440 450
TDPQPSYSAY REASFGHAVL EIYNRTHAYY TWHRNQDNEP VAADSIMLHN
460
RYFFPVEELE SGNTRA
Length:466
Mass (Da):52,969
Last modified:November 1, 1998 - v2
Checksum:iA2AF1C1687922817
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390529 mRNA. Translation: AAQ93684.1.
Z99708 Genomic DNA. Translation: CAB16853.1.
AL022141 Genomic DNA. Translation: CAA18136.1.
AL161589 Genomic DNA. Translation: CAB80301.1.
CP002687 Genomic DNA. Translation: AEE86645.1.
PIRiT04599.
RefSeqiNP_195353.1. NM_119798.1.
UniGeneiAt.31333.

Genome annotation databases

EnsemblPlantsiAT4G36350.1; AT4G36350.1; AT4G36350.
GeneIDi829787.
GrameneiAT4G36350.1; AT4G36350.1; AT4G36350.
KEGGiath:AT4G36350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390529 mRNA. Translation: AAQ93684.1.
Z99708 Genomic DNA. Translation: CAB16853.1.
AL022141 Genomic DNA. Translation: CAA18136.1.
AL161589 Genomic DNA. Translation: CAB80301.1.
CP002687 Genomic DNA. Translation: AEE86645.1.
PIRiT04599.
RefSeqiNP_195353.1. NM_119798.1.
UniGeneiAt.31333.

3D structure databases

ProteinModelPortaliO23244.
SMRiO23244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G36350.1.

PTM databases

iPTMnetiO23244.

Proteomic databases

PaxDbiO23244.
PRIDEiO23244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G36350.1; AT4G36350.1; AT4G36350.
GeneIDi829787.
GrameneiAT4G36350.1; AT4G36350.1; AT4G36350.
KEGGiath:AT4G36350.

Organism-specific databases

TAIRiAT4G36350.

Phylogenomic databases

eggNOGiKOG1378. Eukaryota.
COG1409. LUCA.
HOGENOMiHOG000238330.
InParanoidiO23244.
OMAiEASIDMP.
OrthoDBiEOG09360APJ.
PhylomeDBiO23244.

Enzyme and pathway databases

BioCyciARA:AT4G36350-MONOMER.

Miscellaneous databases

PROiO23244.

Gene expression databases

GenevisibleiO23244. AT.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
PF16656. Pur_ac_phosph_N. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPA25_ARATH
AccessioniPrimary (citable) accession number: O23244
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1998
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.