Reviewed, UniProtKB/Swiss-Prot O23244 (PPA25_ARATH)

Names and origin

Protein names

Recommended name:
    Purple acid phosphatase 25
    EC=3.1.3.2

Gene names

Name:	PAP25
Synonyms:	AT7, ATH2
Ordered Locus Names:	At4g36350
ORF Names:	C7A10.1010, F23E13.190

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Specifically expressed in flowers. Ref.1
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cell wall Inferred from direct assay. Source: TAIR extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 466	445	Purple acid phosphatase 25		PRO_0000372828
Regions
	Region	351 – 353	3	Substrate binding By similarity
Sites
	Active site	324	1	Proton donor By similarity
	Metal binding	164	1	Iron By similarity
	Metal binding	192	1	Iron By similarity
	Metal binding	192	1	Zinc By similarity
	Metal binding	195	1	Iron By similarity
	Metal binding	229	1	Zinc By similarity
	Metal binding	314	1	Zinc By similarity
	Metal binding	351	1	Zinc By similarity
	Metal binding	353	1	Iron By similarity
	Binding site	229	1	Substrate By similarity
Amino acid modifications
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	424	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O23244-1 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: A2AF1C1687922817	FASTA	466	52,969
	10 20 30 40 50 60 MRMNKILLVF VFLSIATVIN SGTTSNFVRT AQPSTEMSLE TFPSPAGHNA PEQVHIVQGD 70 80 90 100 110 120 YNGRGIIISW VTPLNLAGSN VVTYWKAVDG DVKPKKKRGH ASTSSYRFYD YTSGFLHHAT 130 140 150 160 170 180 IKGLEYDTKY IYEVGTDGSV RQFSFTSPPK VGPDVPYTFG IIGDLGQTLA SNETLYHYMS 190 200 210 220 230 240 NPKGQAVLFP GDLSYADDHP NHDQRKWDSW GRFVEPCAAY QTFIYAAGNH EIDFVPNIGE 250 260 270 280 290 300 PHAFKPYIHR YHNAYKASKS ISPLWYSIRR ASAHIIVLSS YSAYGKYTPQ YVWLEQELKK 310 320 330 340 350 360 VNREETPWLI VMVHSPWYNS NNYHYMEGES MRAMFESWFV NSKVDLVLSG HVHSYERSER 370 380 390 400 410 420 VSNIKYNITN GLSYPVKDPS APIYITIGDG GNIEGIANSF TDPQPSYSAY REASFGHAVL 430 440 450 460 EIYNRTHAYY TWHRNQDNEP VAADSIMLHN RYFFPVEELE SGNTRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed: 16244908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed: 12021284] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY390529 mRNA. Translation: AAQ93684.1. Z99708 Genomic DNA. Translation: CAB16853.1. AL022141 Genomic DNA. Translation: CAA18136.1. AL161589 Genomic DNA. Translation: CAB80301.1.
IPI	IPI00536177.
PIR	T04599.
RefSeq	NP_195353.1.
UniGene	At.31333
3D structure databases
HSSP	HSSP built from PDB template 4KBP based on UniProtKB P80366.
ModBase	Search...
Proteomic databases
PRIDE	O23244.
Genome annotation databases
GeneID	829787.
GenomeReviews	Gene locus AT4G36350 in contig CT486007_GR.
KEGG	ath:AT4G36350.
NMPDR	fig|3702.1.peg.21728.
Organism-specific databases
TAIR	At4g36350.
Phylogenomic databases
OMA	SIATVIN.
Gene expression databases
Genevestigator	O23244.
Family and domain databases
InterPro	IPR004843. M-pesterase. IPR015914. Purple_acid_Pase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.380. Purple_acid_Pase_N. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA25_ARATH

Accession

Primary (citable) accession number: O23244

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)