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Protein

Purple acid phosphatase 25

Gene

PAP25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi164IronBy similarity1
Metal bindingi192IronBy similarity1
Metal bindingi192ZincBy similarity1
Metal bindingi195IronBy similarity1
Metal bindingi229ZincBy similarity1
Binding sitei229SubstrateBy similarity1
Metal bindingi314ZincBy similarity1
Active sitei324Proton donorBy similarity1
Metal bindingi351ZincBy similarity1
Metal bindingi353IronBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: TAIR

GO - Biological processi

  • phosphate-containing compound metabolic process Source: TAIR

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT4G36350-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 25 (EC:3.1.3.2)
Gene namesi
Name:PAP25
Synonyms:AT7, ATH2
Ordered Locus Names:At4g36350
ORF Names:C7A10.1010, F23E13.190
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G36350
TAIRilocus:2115345 AT4G36350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000037282822 – 466Purple acid phosphatase 25Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi424N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO23244
PRIDEiO23244

PTM databases

iPTMnetiO23244

Expressioni

Tissue specificityi

Specifically expressed in flowers.1 Publication

Gene expression databases

ExpressionAtlasiO23244 baseline and differential
GenevisibleiO23244 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G36350.1

Structurei

3D structure databases

ProteinModelPortaliO23244
SMRiO23244
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni351 – 353Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378 Eukaryota
COG1409 LUCA
HOGENOMiHOG000238330
InParanoidiO23244
KOiK22390
OMAiFPSPAGH
OrthoDBiEOG09360APJ
PhylomeDBiO23244

Family and domain databases

Gene3Di2.60.40.380, 1 hit
3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR008963 Purple_acid_Pase-like_N
IPR015914 Purple_acid_Pase_N
IPR025733 Purple_acid_PPase_C_dom
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF14008 Metallophos_C, 1 hit
PF16656 Pur_ac_phosph_N, 1 hit
SUPFAMiSSF49363 SSF49363, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRMNKILLVF VFLSIATVIN SGTTSNFVRT AQPSTEMSLE TFPSPAGHNA
60 70 80 90 100
PEQVHIVQGD YNGRGIIISW VTPLNLAGSN VVTYWKAVDG DVKPKKKRGH
110 120 130 140 150
ASTSSYRFYD YTSGFLHHAT IKGLEYDTKY IYEVGTDGSV RQFSFTSPPK
160 170 180 190 200
VGPDVPYTFG IIGDLGQTLA SNETLYHYMS NPKGQAVLFP GDLSYADDHP
210 220 230 240 250
NHDQRKWDSW GRFVEPCAAY QTFIYAAGNH EIDFVPNIGE PHAFKPYIHR
260 270 280 290 300
YHNAYKASKS ISPLWYSIRR ASAHIIVLSS YSAYGKYTPQ YVWLEQELKK
310 320 330 340 350
VNREETPWLI VMVHSPWYNS NNYHYMEGES MRAMFESWFV NSKVDLVLSG
360 370 380 390 400
HVHSYERSER VSNIKYNITN GLSYPVKDPS APIYITIGDG GNIEGIANSF
410 420 430 440 450
TDPQPSYSAY REASFGHAVL EIYNRTHAYY TWHRNQDNEP VAADSIMLHN
460
RYFFPVEELE SGNTRA
Length:466
Mass (Da):52,969
Last modified:November 1, 1998 - v2
Checksum:iA2AF1C1687922817
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY390529 mRNA Translation: AAQ93684.1
Z99708 Genomic DNA Translation: CAB16853.1
AL022141 Genomic DNA Translation: CAA18136.1
AL161589 Genomic DNA Translation: CAB80301.1
CP002687 Genomic DNA Translation: AEE86645.1
PIRiT04599
RefSeqiNP_195353.1, NM_119798.1
UniGeneiAt.31333

Genome annotation databases

EnsemblPlantsiAT4G36350.1; AT4G36350.1; AT4G36350
GeneIDi829787
GrameneiAT4G36350.1; AT4G36350.1; AT4G36350
KEGGiath:AT4G36350

Similar proteinsi

Entry informationi

Entry nameiPPA25_ARATH
AccessioniPrimary (citable) accession number: O23244
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health