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Protein

Mitogen-activated protein kinase 14

Gene

MPK14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 14 (EC:2.7.11.24)
Short name:
AtMPK14
Short name:
MAP kinase 14
Gene namesi
Name:MPK14
Ordered Locus Names:At4g36450
ORF Names:AP22.98, C7A10.910
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G36450.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002458141 – 361Mitogen-activated protein kinase 14Add BLAST361

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphothreonineBy similarity1
Modified residuei190PhosphotyrosineBy similarity1
Modified residuei193PhosphothreonineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-188 and Tyr-190, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO23236.

Expressioni

Gene expression databases

ExpressionAtlasiO23236. baseline and differential.
GenevisibleiO23236. AT.

Interactioni

Subunit structurei

Interacts with MKK3.1 Publication

Protein-protein interaction databases

IntActiO23236. 1 interactor.
STRINGi3702.AT4G36450.1.

Structurei

3D structure databases

ProteinModelPortaliO23236.
SMRiO23236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 316Protein kinasePROSITE-ProRule annotationAdd BLAST285

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi188 – 190TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiO23236.
KOiK20537.
OMAiREMMWEE.
OrthoDBiEOG09360CTC.
PhylomeDBiO23236.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMLVDPPNG IRQEGKHYYT MWQTLFEIDT KYVPIKPIGR GAYGVVCSSI
60 70 80 90 100
NSETNERVAI KKIHNVFENR IDALRTLREL KLLRHVRHEN VISLKDVMLP
110 120 130 140 150
THRYSFRDVY LVYELMDSDL NQIIKSSQSL SDDHCKYFLF QLLRGLKYLH
160 170 180 190 200
SANILHRDLK PGNLLVNANC DLKICDFGLA RTYEQFMTEY VVTRWYRAPE
210 220 230 240 250
LLLCCDNYGT SIDVWSVGCI FAEILGRKPI FPGTECLNQL KLIINVVGSQ
260 270 280 290 300
QDWDLQFIDN QKARRFIKSL PFSKGTHFSH IYPHANPLAI DLLQRMLVFD
310 320 330 340 350
PTKRISVSDA LLHPYMEGLL EPECNPSENV PVSSLEIDEN MEGDMIREMM
360
WEEMLHYLPR A
Length:361
Mass (Da):41,976
Last modified:January 1, 1998 - v1
Checksum:iFECE45953F45F738
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99708 Genomic DNA. Translation: CAB16812.1.
AL161589 Genomic DNA. Translation: CAB80311.1.
CP002687 Genomic DNA. Translation: AEE86657.1.
DQ056668 mRNA. Translation: AAY78814.1.
PIRiC85430.
RefSeqiNP_195363.1. NM_119808.2.
UniGeneiAt.54629.

Genome annotation databases

EnsemblPlantsiAT4G36450.1; AT4G36450.1; AT4G36450.
GeneIDi829797.
GrameneiAT4G36450.1; AT4G36450.1; AT4G36450.
KEGGiath:AT4G36450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z99708 Genomic DNA. Translation: CAB16812.1.
AL161589 Genomic DNA. Translation: CAB80311.1.
CP002687 Genomic DNA. Translation: AEE86657.1.
DQ056668 mRNA. Translation: AAY78814.1.
PIRiC85430.
RefSeqiNP_195363.1. NM_119808.2.
UniGeneiAt.54629.

3D structure databases

ProteinModelPortaliO23236.
SMRiO23236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO23236. 1 interactor.
STRINGi3702.AT4G36450.1.

Proteomic databases

PaxDbiO23236.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G36450.1; AT4G36450.1; AT4G36450.
GeneIDi829797.
GrameneiAT4G36450.1; AT4G36450.1; AT4G36450.
KEGGiath:AT4G36450.

Organism-specific databases

TAIRiAT4G36450.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiO23236.
KOiK20537.
OMAiREMMWEE.
OrthoDBiEOG09360CTC.
PhylomeDBiO23236.

Enzyme and pathway databases

ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112409. RAF-independent MAPK1/3 activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-198753. ERK/MAPK targets.
R-ATH-198765. Signalling to ERK5.
R-ATH-202670. ERKs are inactivated.
R-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-442742. CREB phosphorylation through the activation of Ras.
R-ATH-445144. Signal transduction by L1.
R-ATH-5673001. RAF/MAP kinase cascade.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO23236.

Gene expression databases

ExpressionAtlasiO23236. baseline and differential.
GenevisibleiO23236. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK14_ARATH
AccessioniPrimary (citable) accession number: O23236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.