O23193 (CBSX1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CBS domain-containing protein CBSX1, chloroplastic

Alternative name(s):
CBS domain-containing protein 2
Short name=AtCDCP2
Protein LOSS OF THE TIMING OF ET AND JA BIOSYNTHESIS 2
Short name=AtLEJ2

Gene names

Name:	CBSX1
Synonyms:	CDCP2
Ordered Locus Names:	At4g36910
ORF Names:	AP22.61, C7A10.450

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Plastid › chloroplast Ref.7.
Sequence similarities	Contains 2 CBS domains.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	CBS domain Repeat Transit peptide
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from direct assay PubMed 22021414. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay Ref.7PubMed 22021414. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 71

71

Chloroplast Potential

Chain

72 – 236

165

CBS domain-containing protein CBSX1, chloroplastic

PRO_0000403988

Regions

Domain

81 – 142

62

CBS 1

Domain

175 – 231

57

CBS 2

Secondary structure

1

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236

Helix Strand Turn

Details...

Helix

77 – 79

3

Beta strand

81 – 84

4

Helix

96 – 105

10

Beta strand

109 – 114

6

Beta strand

119 – 125

7

Helix

126 – 129

4

Helix

154 – 160

7

Helix

171 – 174

4

Beta strand

175 – 178

4

Beta strand

182 – 187

6

Helix

188 – 197

10

Beta strand

202 – 206

5

Beta strand

210 – 217

8

Helix

218 – 226

9

Sequences

	Sequence		Length	Mass (Da)
	O23193 [UniParc]. Last modified May 1, 1999. Version 2. Checksum: AE5D224F42A997D9	FASTA	236	25,769
	10 20 30 40 50 60 MDAVLYSVPL SFTPLRASSS PSSPYLLLPR FLSVQPCHKF TFSRSFPSKS RIPSASSAAG 70 80 90 100 110 120 STLMTNSSSP RSGVYTVGEF MTKKEDLHVV KPTTTVDEAL ELLVENRITG FPVIDEDWKL 130 140 150 160 170 180 VGLVSDYDLL ALDSISGSGR TENSMFPEVD STWKTFNAVQ KLLSKTNGKL VGDLMTPAPL 190 200 210 220 230 VVEEKTNLED AAKILLETKY RRLPVVDSDG KLVGIITRGN VVRAALQIKR SGDRNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Purification, crystallization and preliminary X-ray diffraction analysis of a cystathionine beta-synthase domain-containing protein, CDCP2, from Arabidopsis thaliana." Jeong B.C., Yoo K.S., Jung K.W., Shin J.S., Song H.K. Acta Crystallogr. F 64:825-827(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CRYSTALLIZATION.
[7]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Genome wide expression analysis of CBS domain containing proteins in Arabidopsis thaliana (L.) Heynh and Oryza sativa L. reveals their developmental and stress regulation." Kushwaha H.R., Singh A.K., Sopory S.K., Singla-Pareek S.L., Pareek A. BMC Genomics 10:200-200(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z99707 Genomic DNA. Translation: CAB16799.1.
AL161590 Genomic DNA. Translation: CAB80357.1.
CP002687 Genomic DNA. Translation: AEE86716.1.
AY085164 mRNA. Translation: AAM61717.1.
BT003835 mRNA. Translation: AAO41886.1.
BT005190 mRNA. Translation: AAO50723.1.

IPI

IPI00526612.

PIR

H85435.

RefSeq

NP_195409.1. NM_119855.3.

UniGene

At.31287.
At.65827.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GQV	X-ray	2.39	A	72-236	[»]
4GQW	X-ray	2.20	A	72-236	[»]

ProteinModelPortal

O23193.

SMR

O23193. Positions 75-232.

ModBase

Search...

Protein-protein interaction databases

IntAct

O23193. 4 interactions.

STRING

3702.AT4G36910.1-P.

Proteomic databases

PaxDb

O23193.

PRIDE

O23193.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT4G36910.1; AT4G36910.1; AT4G36910.

GeneID

829844.

KEGG

ath:AT4G36910.

Organism-specific databases

TAIR

At4g36910.

Phylogenomic databases

eggNOG

COG0517.

HOGENOM

HOG000187907.

InParanoid

O23193.

OMA

GRTENSM.

PhylomeDB

O23193.

ProtClustDB

CLSN2685590.

Gene expression databases

Genevestigator

O23193.

Family and domain databases

InterPro

IPR000644. Cysta_beta_synth_core.
[Graphical view]

Pfam

PF00571. CBS. 2 hits.
[Graphical view]

SMART

SM00116. CBS. 2 hits.
[Graphical view]

PROSITE

PS51371. CBS. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CBSX1_ARATH

Accession

Primary (citable) accession number: O23193

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program