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Protein

Thioredoxin-like protein HCF164, chloroplastic

Gene

HCF164

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thiol-disulfide oxidoreductase that participates in various redox reactions in the chloroplast. Mediates the reduction of PSI-N in the thylakoid lumen. May interact and probably reduce other target proteins of the thylakoid membrane, such as FTSH2, FTSH8, LHCB5, atpA, atpB, atpE, petA and petC. Involved in the biogenesis of the plastid cytochrome b6f complex. Reducing equivalents are provided by stromal M-type thioredoxins and probably transduced through the thylakoid membrane by CCDA. Possesses low insulin disulfide bonds reducing activity.2 Publications

Caution

The active site contains a CEVC motif wich differs from the conserved CGPC motif.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei150NucleophileSequence analysis1
Active sitei153NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: GO_Central
  • cellular response to oxidative stress Source: GO_Central
  • cytochrome b6f complex assembly Source: TAIR
  • glycerol ether metabolic process Source: InterPro

Keywordsi

Biological processElectron transport, Transport

Protein family/group databases

TCDBi3.D.3.5.2 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-like protein HCF164, chloroplastic1 Publication
Alternative name(s):
Protein HIGH CHLOROPHYLL FLUORESCENCE 1641 Publication
Gene namesi
Name:HCF1641 Publication
Ordered Locus Names:At4g37200Imported
ORF Names:C7A10.160Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37200
TAIRilocus:2115110 AT4G37200

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

High chlorophyll fluorescence and deficiency in the accumulation of the cytochrome b6f complex subunits.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40ChloroplastSequence analysisAdd BLAST40
ChainiPRO_000039454341 – 261Thioredoxin-like protein HCF164, chloroplasticAdd BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi150 ↔ 153Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO23166
PRIDEiO23166

PTM databases

iPTMnetiO23166

Expressioni

Gene expression databases

ExpressionAtlasiO23166 baseline and differential
GenevisibleiO23166 AT

Interactioni

Subunit structurei

Interacts in vitro with LTO1.1 Publication

Protein-protein interaction databases

BioGridi15155, 1 interactor
STRINGi3702.AT4G37200.1

Structurei

3D structure databases

ProteinModelPortaliO23166
SMRiO23166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 229ThioredoxinPROSITE-ProRule annotationAdd BLAST129

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi63 – 84Ser-richAdd BLAST22

Sequence similaritiesi

Belongs to the thioredoxin family.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0907 Eukaryota
COG0526 LUCA
HOGENOMiHOG000232501
InParanoidiO23166
OMAiCTSCQAM
OrthoDBiEOG09360JE0
PhylomeDBiO23166

Family and domain databases

InterProiView protein in InterPro
IPR005746 Thioredoxin
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10438 PTHR10438, 1 hit
PfamiView protein in Pfam
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLVFSLNL PSSHGFNLSP RNLQSFFVTQ TGAPRFRAVR CKPNPESSET
60 70 80 90 100
KQEKLVIDNG ETSSASKEVE SSSSVADSSS SSSSGFPESP NKDINRRVAA
110 120 130 140 150
VTVIAALSLF VSTRLDFGIS LKDLTASALP YEEALSNGKP TVVEFYADWC
160 170 180 190 200
EVCRELAPDV YKIEQQYKDK VNFVMLNVDN TKWEQELDEF GVEGIPHFAF
210 220 230 240 250
LDREGNEEGN VVGRLPRQYL VENVNALAAG KQSIPYARAV GQYSSSESRK
260
VHQVTDPLSH G
Length:261
Mass (Da):28,745
Last modified:May 1, 1999 - v2
Checksum:i34FD1A09BD125D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293262 mRNA Translation: CAC19858.1
Z99707 Genomic DNA Translation: CAB16778.1
AL161590 Genomic DNA Translation: CAB80386.1
CP002687 Genomic DNA Translation: AEE86766.1
AY075671 mRNA Translation: AAL77678.1
AY097426 mRNA Translation: AAM19942.1
PIRiE85439
RefSeqiNP_195437.1, NM_119883.3
UniGeneiAt.31265
At.48077

Genome annotation databases

EnsemblPlantsiAT4G37200.1; AT4G37200.1; AT4G37200
GeneIDi829874
GrameneiAT4G37200.1; AT4G37200.1; AT4G37200
KEGGiath:AT4G37200

Similar proteinsi

Entry informationi

Entry nameiTR164_ARATH
AccessioniPrimary (citable) accession number: O23166
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health