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Protein

Thioredoxin-like protein HCF164, chloroplastic

Gene

HCF164

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-disulfide oxidoreductase that participates in various redox reactions in the chloroplast. Mediates the reduction of PSI-N in the thylakoid lumen. May interact and probably reduce other target proteins of the thylakoid membrane, such as FTSH2, FTSH8, LHCB5, atpA, atpB, atpE, petA and petC. Involved in the biogenesis of the plastid cytochrome b6f complex. Reducing equivalents are provided by stromal M-type thioredoxins and probably transduced through the thylakoid membrane by CCDA. Possesses low insulin disulfide bonds reducing activity.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei150 – 1501NucleophileSequence analysis
Active sitei153 – 1531NucleophileSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Protein family/group databases

TCDBi3.D.3.5.2. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-like protein HCF164, chloroplastic1 Publication
Alternative name(s):
Protein HIGH CHLOROPHYLL FLUORESCENCE 1641 Publication
Gene namesi
Name:HCF1641 Publication
Ordered Locus Names:At4g37200Imported
ORF Names:C7A10.160Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G37200.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • thylakoid lumen Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

High chlorophyll fluorescence and deficiency in the accumulation of the cytochrome b6f complex subunits.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040ChloroplastSequence analysisAdd
BLAST
Chaini41 – 261221Thioredoxin-like protein HCF164, chloroplasticPRO_0000394543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi150 ↔ 153Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO23166.
PRIDEiO23166.

PTM databases

iPTMnetiO23166.

Expressioni

Gene expression databases

GenevisibleiO23166. AT.

Interactioni

Subunit structurei

Interacts in vitro with LTO1.1 Publication

Protein-protein interaction databases

BioGridi15155. 1 interaction.
STRINGi3702.AT4G37200.1.

Structurei

3D structure databases

ProteinModelPortaliO23166.
SMRiO23166. Positions 142-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 229129ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi63 – 8422Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the thioredoxin family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000232501.
InParanoidiO23166.
OMAiPKQYLLE.
OrthoDBiEOG09360JE0.
PhylomeDBiO23166.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLVFSLNL PSSHGFNLSP RNLQSFFVTQ TGAPRFRAVR CKPNPESSET
60 70 80 90 100
KQEKLVIDNG ETSSASKEVE SSSSVADSSS SSSSGFPESP NKDINRRVAA
110 120 130 140 150
VTVIAALSLF VSTRLDFGIS LKDLTASALP YEEALSNGKP TVVEFYADWC
160 170 180 190 200
EVCRELAPDV YKIEQQYKDK VNFVMLNVDN TKWEQELDEF GVEGIPHFAF
210 220 230 240 250
LDREGNEEGN VVGRLPRQYL VENVNALAAG KQSIPYARAV GQYSSSESRK
260
VHQVTDPLSH G
Length:261
Mass (Da):28,745
Last modified:May 1, 1999 - v2
Checksum:i34FD1A09BD125D26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293262 mRNA. Translation: CAC19858.1.
Z99707 Genomic DNA. Translation: CAB16778.1.
AL161590 Genomic DNA. Translation: CAB80386.1.
CP002687 Genomic DNA. Translation: AEE86766.1.
AY075671 mRNA. Translation: AAL77678.1.
AY097426 mRNA. Translation: AAM19942.1.
PIRiE85439.
RefSeqiNP_195437.1. NM_119883.3.
UniGeneiAt.31265.
At.48077.

Genome annotation databases

EnsemblPlantsiAT4G37200.1; AT4G37200.1; AT4G37200.
GeneIDi829874.
GrameneiAT4G37200.1; AT4G37200.1; AT4G37200.
KEGGiath:AT4G37200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ293262 mRNA. Translation: CAC19858.1.
Z99707 Genomic DNA. Translation: CAB16778.1.
AL161590 Genomic DNA. Translation: CAB80386.1.
CP002687 Genomic DNA. Translation: AEE86766.1.
AY075671 mRNA. Translation: AAL77678.1.
AY097426 mRNA. Translation: AAM19942.1.
PIRiE85439.
RefSeqiNP_195437.1. NM_119883.3.
UniGeneiAt.31265.
At.48077.

3D structure databases

ProteinModelPortaliO23166.
SMRiO23166. Positions 142-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15155. 1 interaction.
STRINGi3702.AT4G37200.1.

Protein family/group databases

TCDBi3.D.3.5.2. the proton-translocating quinol:cytochrome c reductase (qcr) superfamily.

PTM databases

iPTMnetiO23166.

Proteomic databases

PaxDbiO23166.
PRIDEiO23166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37200.1; AT4G37200.1; AT4G37200.
GeneIDi829874.
GrameneiAT4G37200.1; AT4G37200.1; AT4G37200.
KEGGiath:AT4G37200.

Organism-specific databases

TAIRiAT4G37200.

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000232501.
InParanoidiO23166.
OMAiPKQYLLE.
OrthoDBiEOG09360JE0.
PhylomeDBiO23166.

Miscellaneous databases

PROiO23166.

Gene expression databases

GenevisibleiO23166. AT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTR164_ARATH
AccessioniPrimary (citable) accession number: O23166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

The active site contains a CEVC motif wich differs from the conserved CGPC motif.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.