O23147 (ADPG1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase ADPG1
Short name=AtADPG1
Short name=PG ADPG1
EC=3.2.1.15

Alternative name(s):
Pectinase ADPG1
Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1

Gene names

Name:	ADPG1
Synonyms:	PGDZAT, SAC70
Ordered Locus Names:	At3g57510
ORF Names:	T8H10.110

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polygalacturonase involved in cell separation in the final stages of pod shatter and in anther dehiscence. Not involved in floral organ abscission. Ref.8 Ref.9
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall. Cytoplasm. Note: Released to the cell wall during maturation of the dehiscence zone. Ref.2
Tissue specificity	Expressed in flower buds and siliques, in the dehiscence zone of anthers (stomium cells) and maturing siliques. Expressed in stigma during pollen tube growth. Not expressed in seeds or in the floral part or leaf abscission zone but found at the junction between the seed and the funiculus at the site of seed abscission. Ref.1 Ref.2 Ref.7 Ref.8 Ref.9
Disruption phenotype	Impaired pod shatter. Ref.9
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Cytoplasm Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anther dehiscence Inferred from mutant phenotype Ref.9. Source: TAIR carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall modification involved in abscission Traceable author statement Ref.9. Source: TAIR fruit dehiscence Inferred from mutant phenotype Ref.9. Source: TAIR
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from direct assay Ref.9. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 431	408	Polygalacturonase ADPG1		PRO_0000367913
Regions
	Repeat	223 – 249	27	PbH1 1
	Repeat	250 – 271	22	PbH1 2
	Repeat	303 – 324	22	PbH1 3
	Repeat	332 – 353	22	PbH1 4
	Repeat	398 – 420	23	PbH1 5
Sites
	Active site	264	1	Proton donor By similarity
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23147 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 0F0F30DF45804FE7	FASTA	431	46,572
	10 20 30 40 50 60 MARCCRHLAV FLCVLLMLSL CKALSSNVDD GYGHEDGSFE SDSLLKLNND DVLSLISSDE 70 80 90 100 110 120 TTLEASTVSV SNFGAKGDGK TDDTQAFKKA WKKACSTNGV TTFLVPKGKT YLLKSTRFRG 130 140 150 160 170 180 PCKSLRNFQI LGTLSASTKR SDYKDKNHWL ILEDVNNLSI DGGSTGIING NGKTWWQNSC 190 200 210 220 230 240 KIDKSKPCTK APTALTLYNL KNLNVKNLRV KNAQQIQISI EKCNKVEVSN VEITAPGDSP 250 260 270 280 290 300 NTDGIHITNT QNIRVSNSDI GTGDDCISIE DGTQNLQIFD LTCGPGHGIS IGSLGDDNSK 310 320 330 340 350 360 AYVSGINVDG AKFSESDNGV RIKTYQGGSG TAKNIKFQNI RMENVKNPII IDQDYCDKDK 370 380 390 400 410 420 CEDQESAVQV KNVVYKNISG TSATDVAITL NCSEKYPCQG IVLENVKIKG GTASCKNANV 430 KNQGTVSPKC S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus." Jenkins E.S., Paul W., Craze M., Whitelaw C.A., Weigand A., Roberts J.A. Plant Cell Environ. 22:159-167(1999) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: cv. Landsberg erecta.
[2]	"Analysis of a dehiscence zone endo-polygalacturonase in oilseed rape (Brassica napus) and Arabidopsis thaliana: evidence for roles in cell separation in dehiscence and abscission zones, and in stylar tissues during pollen tube growth." Sander L., Child R., Ulvskov P., Albrechtsen M., Borkhardt B. Plant Mol. Biol. 46:469-479(2001) [PubMed: 11485203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana." Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A. J. Exp. Bot. 58:3719-3730(2007) [PubMed: 17928369] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[9]	"ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are polygalacturonases required for cell separation during reproductive development in Arabidopsis." Ogawa M., Kay P., Wilson S., Swain S.M. Plant Cell 21:216-233(2009) [PubMed: 19168715] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF037367 Genomic DNA. Translation: AAC98923.1. AJ002532 Genomic DNA. Translation: CAA05525.1. AL133248 Genomic DNA. Translation: CAB66108.1. CP002686 Genomic DNA. Translation: AEE79664.1. AK117942 mRNA. Translation: BAC42580.1. BT005376 mRNA. Translation: AAO63440.1.
IPI	IPI00526588.
PIR	T46187.
RefSeq	NP_191310.1. NM_115611.2.
UniGene	At.26895.
3D structure databases
ProteinModelPortal	O23147.
SMR	O23147. Positions 66-421.
ModBase	Search...
Protein-protein interaction databases
STRING	O23147.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	O23147.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G57510.1; AT3G57510.1; AT3G57510.
GeneID	824918.
GenomeReviews	Gene locus AT3G57510 in contig BA000014_GR.
KEGG	ath:AT3G57510.
NMPDR	fig|3702.1.peg.17085.
Organism-specific databases
GeneFarm	24. 2.
TAIR	At3g57510.
Phylogenomic databases
eggNOG	euNOG04659.
GeneTree	EPGT00050000001253.
HOGENOM	HBG744658.
InParanoid	O23147.
OMA	KNIKFQN.
PhylomeDB	O23147.
ProtClustDB	PLN02218.
Gene expression databases
Genevestigator	O23147.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01184.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPG1_ARATH

Accession

Primary (citable) accession number: O23147

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program