Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polygalacturonase ADPG1

Gene

ADPG1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Polygalacturonase involved in cell separation in the final stages of pod shatter and in anther dehiscence. Not involved in floral organ abscission.2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264Proton donorPROSITE-ProRule annotation1
Active sitei287PROSITE-ProRule annotation1

GO - Molecular functioni

  • polygalacturonase activity Source: TAIR

GO - Biological processi

  • anther dehiscence Source: TAIR
  • carbohydrate metabolic process Source: InterPro
  • cell wall modification involved in abscission Source: TAIR
  • fruit dehiscence Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G57510-MONOMER.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase ADPG1 (EC:3.2.1.15)
Short name:
AtADPG1
Short name:
PG ADPG1
Alternative name(s):
Pectinase ADPG1
Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1
Gene namesi
Name:ADPG1
Synonyms:PGDZAT, SAC70
Ordered Locus Names:At3g57510
ORF Names:T8H10.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G57510.

Subcellular locationi

  • Secretedcell wall 1 Publication
  • Cytoplasm 1 Publication

  • Note: Released to the cell wall during maturation of the dehiscence zone.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted

Pathology & Biotechi

Disruption phenotypei

Impaired pod shatter.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000036791324 – 431Polygalacturonase ADPG1Add BLAST408

Proteomic databases

PaxDbiO23147.

Expressioni

Tissue specificityi

Expressed in flower buds and siliques, in the dehiscence zone of anthers (stomium cells) and maturing siliques. Expressed in stigma during pollen tube growth. Not expressed in seeds or in the floral part or leaf abscission zone but found at the junction between the seed and the funiculus at the site of seed abscission.5 Publications

Gene expression databases

GenevisibleiO23147. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G57510.1.

Structurei

3D structure databases

ProteinModelPortaliO23147.
SMRiO23147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati223 – 249PbH1 1Add BLAST27
Repeati250 – 271PbH1 2Add BLAST22
Repeati303 – 324PbH1 3Add BLAST22
Repeati332 – 353PbH1 4Add BLAST22
Repeati398 – 420PbH1 5Add BLAST23

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiO23147.
KOiK01184.
OMAiATPCKEQ.
OrthoDBiEOG09360ADQ.
PhylomeDBiO23147.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O23147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARCCRHLAV FLCVLLMLSL CKALSSNVDD GYGHEDGSFE SDSLLKLNND
60 70 80 90 100
DVLSLISSDE TTLEASTVSV SNFGAKGDGK TDDTQAFKKA WKKACSTNGV
110 120 130 140 150
TTFLVPKGKT YLLKSTRFRG PCKSLRNFQI LGTLSASTKR SDYKDKNHWL
160 170 180 190 200
ILEDVNNLSI DGGSTGIING NGKTWWQNSC KIDKSKPCTK APTALTLYNL
210 220 230 240 250
KNLNVKNLRV KNAQQIQISI EKCNKVEVSN VEITAPGDSP NTDGIHITNT
260 270 280 290 300
QNIRVSNSDI GTGDDCISIE DGTQNLQIFD LTCGPGHGIS IGSLGDDNSK
310 320 330 340 350
AYVSGINVDG AKFSESDNGV RIKTYQGGSG TAKNIKFQNI RMENVKNPII
360 370 380 390 400
IDQDYCDKDK CEDQESAVQV KNVVYKNISG TSATDVAITL NCSEKYPCQG
410 420 430
IVLENVKIKG GTASCKNANV KNQGTVSPKC S
Length:431
Mass (Da):46,572
Last modified:January 1, 1998 - v1
Checksum:i0F0F30DF45804FE7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037367 Genomic DNA. Translation: AAC98923.1.
AJ002532 Genomic DNA. Translation: CAA05525.1.
AL133248 Genomic DNA. Translation: CAB66108.1.
CP002686 Genomic DNA. Translation: AEE79664.1.
AK117942 mRNA. Translation: BAC42580.1.
BT005376 mRNA. Translation: AAO63440.1.
PIRiT46187.
RefSeqiNP_191310.1. NM_115611.2.
UniGeneiAt.26895.

Genome annotation databases

EnsemblPlantsiAT3G57510.1; AT3G57510.1; AT3G57510.
GeneIDi824918.
GrameneiAT3G57510.1; AT3G57510.1; AT3G57510.
KEGGiath:AT3G57510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037367 Genomic DNA. Translation: AAC98923.1.
AJ002532 Genomic DNA. Translation: CAA05525.1.
AL133248 Genomic DNA. Translation: CAB66108.1.
CP002686 Genomic DNA. Translation: AEE79664.1.
AK117942 mRNA. Translation: BAC42580.1.
BT005376 mRNA. Translation: AAO63440.1.
PIRiT46187.
RefSeqiNP_191310.1. NM_115611.2.
UniGeneiAt.26895.

3D structure databases

ProteinModelPortaliO23147.
SMRiO23147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G57510.1.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PaxDbiO23147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G57510.1; AT3G57510.1; AT3G57510.
GeneIDi824918.
GrameneiAT3G57510.1; AT3G57510.1; AT3G57510.
KEGGiath:AT3G57510.

Organism-specific databases

TAIRiAT3G57510.

Phylogenomic databases

eggNOGiENOG410IF8F. Eukaryota.
COG5434. LUCA.
HOGENOMiHOG000237792.
InParanoidiO23147.
KOiK01184.
OMAiATPCKEQ.
OrthoDBiEOG09360ADQ.
PhylomeDBiO23147.

Enzyme and pathway databases

BioCyciARA:AT3G57510-MONOMER.

Miscellaneous databases

PROiO23147.

Gene expression databases

GenevisibleiO23147. AT.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADPG1_ARATH
AccessioniPrimary (citable) accession number: O23147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.