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Protein

AP-2 complex subunit mu

Gene

AP2M

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 recognizes Y-X-X-Phi endocytosis signal motif within the cytosolic tails of transmembrane cargo molecules. The complex binds polyphosphoinositides.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei348 – 3481Phosphatidylinositol lipid headgroupBy similarity
Binding sitei359 – 3591Phosphatidylinositol lipid headgroupBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit mu
Alternative name(s):
Adaptor protein complex AP-2 subunit mu
Adaptor protein-2 mu-adaptin
Adaptor-related protein complex 2 subunit mu
At-muA-Ad
Clathrin assembly protein complex 2 mu medium chain
Mu2-adaptin
Protein AP47/50
Short name:
AtAP47/50
Gene namesi
Name:AP2M
Synonyms:AP47/50
Ordered Locus Names:At5g46630
ORF Names:MZA15.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G46630.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438AP-2 complex subunit muPRO_0000424262Add
BLAST

Proteomic databases

PaxDbiO23140.
PRIDEiO23140.

Expressioni

Gene expression databases

ExpressionAtlasiO23140. baseline and differential.
GenevisibleiO23140. AT.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type and beta-type subunits), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).By similarity

Protein-protein interaction databases

BioGridi19954. 161 interactions.
STRINGi3702.AT5G46630.2.

Structurei

3D structure databases

ProteinModelPortaliO23140.
SMRiO23140. Positions 7-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini177 – 437261MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0938. Eukaryota.
ENOG410XPFS. LUCA.
HOGENOMiHOG000173246.
KOiK11826.
PhylomeDBiO23140.

Family and domain databases

InterProiIPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O23140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAASAIYF LNLRGDVLIN RTYRDDVGGN MVDAFRTHIM QTKELGNCPV
60 70 80 90 100
RQIGGCSFVY MRISNVYIVI VVSSNANVAC GFKFVVEAVA LFKSYFGGAF
110 120 130 140 150
DEDAIRNNFV LIYELLDEIM DFGYPQNLSP EILKLYITQE GVRSPFSSKP
160 170 180 190 200
KDKPVPNATL QVTGAVGWRR EGLAYKKNEV FLDIVESVNL LMSSKGNVLR
210 220 230 240 250
CDVTGKVLMK CFLSGMPDLK LGLNDKIGLE KESEMKSRPA KSGKTIELDD
260 270 280 290 300
VTFHQCVNLT RFNSEKTVSF VPPDGEFELM KYRITEGVNL PFRVLPTIKE
310 320 330 340 350
LGRTRMEVNV KVKSVFGAKM FALGVVVKIP VPKQTAKTNF QVTTGRAKYN
360 370 380 390 400
PSIDCLVWKI RKFPGQTEST LSAEIELIST MGEKKSWTRP PIQMEFQVPM
410 420 430
FTASGLRVRF LKVWEKSGYN TVEWVRYITK AGSYEIRC
Length:438
Mass (Da):49,322
Last modified:January 1, 1998 - v1
Checksum:iBF08ED27109CBFF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009631 mRNA. Translation: AAB88283.1.
AB016882 Genomic DNA. Translation: BAB08907.1.
CP002688 Genomic DNA. Translation: AED95407.1.
AK317765 mRNA. Translation: BAH20421.1.
RefSeqiNP_199475.1. NM_124033.3. [O23140-1]
UniGeneiAt.23058.

Genome annotation databases

EnsemblPlantsiAT5G46630.1; AT5G46630.1; AT5G46630. [O23140-1]
GeneIDi834706.
KEGGiath:AT5G46630.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009631 mRNA. Translation: AAB88283.1.
AB016882 Genomic DNA. Translation: BAB08907.1.
CP002688 Genomic DNA. Translation: AED95407.1.
AK317765 mRNA. Translation: BAH20421.1.
RefSeqiNP_199475.1. NM_124033.3. [O23140-1]
UniGeneiAt.23058.

3D structure databases

ProteinModelPortaliO23140.
SMRiO23140. Positions 7-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19954. 161 interactions.
STRINGi3702.AT5G46630.2.

Proteomic databases

PaxDbiO23140.
PRIDEiO23140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G46630.1; AT5G46630.1; AT5G46630. [O23140-1]
GeneIDi834706.
KEGGiath:AT5G46630.

Organism-specific databases

TAIRiAT5G46630.

Phylogenomic databases

eggNOGiKOG0938. Eukaryota.
ENOG410XPFS. LUCA.
HOGENOMiHOG000173246.
KOiK11826.
PhylomeDBiO23140.

Miscellaneous databases

PROiO23140.

Gene expression databases

ExpressionAtlasiO23140. baseline and differential.
GenevisibleiO23140. AT.

Family and domain databases

InterProiIPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP2M_ARATH
AccessioniPrimary (citable) accession number: O23140
Secondary accession number(s): B9DI54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.