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Protein

AP-2 complex subunit mu

Gene

AP2M

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 recognizes Y-X-X-Phi endocytosis signal motif within the cytosolic tails of transmembrane cargo molecules. The complex binds polyphosphoinositides.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei348Phosphatidylinositol lipid headgroupBy similarity1
Binding sitei359Phosphatidylinositol lipid headgroupBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-ATH-8856825 Cargo recognition for clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit mu
Alternative name(s):
Adaptor protein complex AP-2 subunit mu
Adaptor protein-2 mu-adaptin
Adaptor-related protein complex 2 subunit mu
At-muA-Ad
Clathrin assembly protein complex 2 mu medium chain
Mu2-adaptin
Protein AP47/50
Short name:
AtAP47/50
Gene namesi
Name:AP2M
Synonyms:AP47/50
Ordered Locus Names:At5g46630
ORF Names:MZA15.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G46630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004242621 – 438AP-2 complex subunit muAdd BLAST438

Proteomic databases

PaxDbiO23140
PRIDEiO23140

Expressioni

Gene expression databases

ExpressionAtlasiO23140 baseline and differential
GenevisibleiO23140 AT

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type and beta-type subunits), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).By similarity

Protein-protein interaction databases

BioGridi19954, 161 interactors
STRINGi3702.AT5G46630.2

Structurei

3D structure databases

ProteinModelPortaliO23140
SMRiO23140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 437MHDPROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0938 Eukaryota
ENOG410XPFS LUCA
HOGENOMiHOG000173246
KOiK11826
PhylomeDBiO23140

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS51072 MHD, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O23140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVAASAIYF LNLRGDVLIN RTYRDDVGGN MVDAFRTHIM QTKELGNCPV
60 70 80 90 100
RQIGGCSFVY MRISNVYIVI VVSSNANVAC GFKFVVEAVA LFKSYFGGAF
110 120 130 140 150
DEDAIRNNFV LIYELLDEIM DFGYPQNLSP EILKLYITQE GVRSPFSSKP
160 170 180 190 200
KDKPVPNATL QVTGAVGWRR EGLAYKKNEV FLDIVESVNL LMSSKGNVLR
210 220 230 240 250
CDVTGKVLMK CFLSGMPDLK LGLNDKIGLE KESEMKSRPA KSGKTIELDD
260 270 280 290 300
VTFHQCVNLT RFNSEKTVSF VPPDGEFELM KYRITEGVNL PFRVLPTIKE
310 320 330 340 350
LGRTRMEVNV KVKSVFGAKM FALGVVVKIP VPKQTAKTNF QVTTGRAKYN
360 370 380 390 400
PSIDCLVWKI RKFPGQTEST LSAEIELIST MGEKKSWTRP PIQMEFQVPM
410 420 430
FTASGLRVRF LKVWEKSGYN TVEWVRYITK AGSYEIRC
Length:438
Mass (Da):49,322
Last modified:January 1, 1998 - v1
Checksum:iBF08ED27109CBFF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009631 mRNA Translation: AAB88283.1
AB016882 Genomic DNA Translation: BAB08907.1
CP002688 Genomic DNA Translation: AED95407.1
AK317765 mRNA Translation: BAH20421.1
RefSeqiNP_199475.1, NM_124033.4 [O23140-1]
UniGeneiAt.23058

Genome annotation databases

EnsemblPlantsiAT5G46630.1; AT5G46630.1; AT5G46630 [O23140-1]
GeneIDi834706
GrameneiAT5G46630.1; AT5G46630.1; AT5G46630 [O23140-1]
KEGGiath:AT5G46630

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAP2M_ARATH
AccessioniPrimary (citable) accession number: O23140
Secondary accession number(s): B9DI54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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