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Protein

Calcium-transporting ATPase 2, endoplasmic reticulum-type

Gene

ECA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi321Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi322Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi324Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi326Calcium 2By similarity1
Active sitei3684-aspartylphosphate intermediateBy similarity1
Metal bindingi727MagnesiumBy similarity1
Metal bindingi731MagnesiumBy similarity1
Metal bindingi793Calcium 1By similarity1
Metal bindingi796Calcium 1By similarity1
Metal bindingi821Calcium 2By similarity1
Metal bindingi824Calcium 1By similarity1
Metal bindingi825Calcium 1By similarity1
Metal bindingi825Calcium 2By similarity1
Metal bindingi960Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • calcium ion transport Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G00900-MONOMER.
MetaCyc:MONOMER-14601.
BRENDAi3.6.3.8. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 2, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:ECA2
Synonyms:ACA5
Ordered Locus Names:At4g00900
ORF Names:A_TM018A10.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G00900.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 53CytoplasmicSequence analysisAdd BLAST53
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 98LumenalSequence analysisAdd BLAST24
Transmembranei99 – 118HelicalSequence analysisAdd BLAST20
Topological domaini119 – 262CytoplasmicSequence analysisAdd BLAST144
Transmembranei263 – 282HelicalSequence analysisAdd BLAST20
Topological domaini283 – 312LumenalSequence analysisAdd BLAST30
Transmembranei313 – 330HelicalSequence analysisAdd BLAST18
Topological domaini331 – 782CytoplasmicSequence analysisAdd BLAST452
Transmembranei783 – 802HelicalSequence analysisAdd BLAST20
Topological domaini803 – 812LumenalSequence analysis10
Transmembranei813 – 833HelicalSequence analysisAdd BLAST21
Topological domaini834 – 853CytoplasmicSequence analysisAdd BLAST20
Transmembranei854 – 876HelicalSequence analysisAdd BLAST23
Topological domaini877 – 949LumenalSequence analysisAdd BLAST73
Transmembranei950 – 969HelicalSequence analysisAdd BLAST20
Topological domaini970 – 982CytoplasmicSequence analysisAdd BLAST13
Transmembranei983 – 1001HelicalSequence analysisAdd BLAST19
Topological domaini1002 – 1016LumenalSequence analysisAdd BLAST15
Transmembranei1017 – 1037HelicalSequence analysisAdd BLAST21
Topological domaini1038 – 1054CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464061 – 1054Calcium-transporting ATPase 2, endoplasmic reticulum-typeAdd BLAST1054

Proteomic databases

PaxDbiO23087.
PRIDEiO23087.

PTM databases

SwissPalmiO23087.

Expressioni

Gene expression databases

GenevisibleiO23087. AT.

Interactioni

Protein-protein interaction databases

BioGridi13275. 4 interactors.
MINTiMINT-8065871.
STRINGi3702.AT4G00900.1.

Structurei

3D structure databases

ProteinModelPortaliO23087.
SMRiO23087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO23087.
KOiK01537.
OMAiIAMWASE.
OrthoDBiEOG093600UE.
PhylomeDBiO23087.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEKSFSAW SWSVEQCLKE YKTRLDKGLT SEDVQIRRQK YGFNELAKEK
60 70 80 90 100
GKPLWHLVLE QFDDTLVKIL LGAAFISFVL AFLGEEHGSG SGFEAFVEPF
110 120 130 140 150
VIVLILILNA VVGVWQESNA EKALEALKEM QCESAKVLRD GNVLPNLPAR
160 170 180 190 200
ELVPGDIVEL NVGDKVPADM RVSGLKTSTL RVEQSSLTGE AMPVLKGANL
210 220 230 240 250
VVMDDCELQG KENMVFAGTT VVNGSCVCIV TSIGMDTEIG KIQRQIHEAS
260 270 280 290 300
LEESETPLKK KLDEFGSRLT TAICIVCVLV WMINYKNFVS WDVVDGYKPV
310 320 330 340 350
NIKFSFEKCT YYFKIAVALA VAAIPEGLPA VITTCLALGT RKMAQKNAIV
360 370 380 390 400
RKLPSVETLG CTTVICSDKT GTLTTNQMSA TEFFTLGGKT TTTRVFSVSG
410 420 430 440 450
TTYDPKDGGI VDWGCNNMDA NLQAVAEICS ICNDAGVFYE GKLFRATGLP
460 470 480 490 500
TEAALKVLVE KMGIPEKKNS ENIEEVTNFS DNGSSVKLAC CDWWNKRSKK
510 520 530 540 550
VATLEFDRVR KSMSVIVSEP NGQNRLLVKG AAESILERSS FAQLADGSLV
560 570 580 590 600
ALDESSREVI LKKHSEMTSK GLRCLGLAYK DELGEFSDYS SEEHPSHKKL
610 620 630 640 650
LDPSSYSNIE TNLIFVGVVG LRDPPREEVG RAIEDCRDAG IRVMVITGDN
660 670 680 690 700
KSTAEAICCE IRLFSENEDL SQSSFTGKEF MSLPASRRSE ILSKSGGKVF
710 720 730 740 750
SRAEPRHKQE IVRMLKEMGE IVAMTGDGVN DAPALKLADI GIAMGITGTE
760 770 780 790 800
VAKEASDMVL ADDNFSTIVS AVAEGRSIYN NMKAFIRYMI SSNVGEVISI
810 820 830 840 850
FLTAALGIPE CMIPVQLLWV NLVTDGPPAT ALGFNPADID IMKKPPRKSD
860 870 880 890 900
DCLIDSWVLI RYLVIGSYVG VATVGIFVLW YTQASFLGIS LISDGHTLVS
910 920 930 940 950
FTQLQNWSEC SSWGTNFTAT PYTVAGGLRT IAFENNPCDY FTLGKVKPMT
960 970 980 990 1000
LSLTVLVAIE MFNSLNALSE DNSLLTMPPW RNPWLLVAMT VSFALHCVIL
1010 1020 1030 1040 1050
YVPFLANVFG IVPLSFREWF VVILVSFPVI LIDEALKFIG RCRRTRIKKK

IKTM
Length:1,054
Mass (Da):115,830
Last modified:January 1, 1998 - v1
Checksum:iB0D7F7237A3496AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132387 mRNA. Translation: CAA10659.1.
AF013294 Genomic DNA. Translation: AAB62850.1.
AL161472 Genomic DNA. Translation: CAB80899.1.
CP002687 Genomic DNA. Translation: AEE81953.1.
PIRiT01556.
RefSeqiNP_191999.1. NM_116317.4.
UniGeneiAt.488.

Genome annotation databases

EnsemblPlantsiAT4G00900.1; AT4G00900.1; AT4G00900.
GeneIDi827984.
GrameneiAT4G00900.1; AT4G00900.1; AT4G00900.
KEGGiath:AT4G00900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132387 mRNA. Translation: CAA10659.1.
AF013294 Genomic DNA. Translation: AAB62850.1.
AL161472 Genomic DNA. Translation: CAB80899.1.
CP002687 Genomic DNA. Translation: AEE81953.1.
PIRiT01556.
RefSeqiNP_191999.1. NM_116317.4.
UniGeneiAt.488.

3D structure databases

ProteinModelPortaliO23087.
SMRiO23087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13275. 4 interactors.
MINTiMINT-8065871.
STRINGi3702.AT4G00900.1.

PTM databases

SwissPalmiO23087.

Proteomic databases

PaxDbiO23087.
PRIDEiO23087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G00900.1; AT4G00900.1; AT4G00900.
GeneIDi827984.
GrameneiAT4G00900.1; AT4G00900.1; AT4G00900.
KEGGiath:AT4G00900.

Organism-specific databases

TAIRiAT4G00900.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO23087.
KOiK01537.
OMAiIAMWASE.
OrthoDBiEOG093600UE.
PhylomeDBiO23087.

Enzyme and pathway databases

BioCyciARA:AT4G00900-MONOMER.
MetaCyc:MONOMER-14601.
BRENDAi3.6.3.8. 399.

Miscellaneous databases

PROiO23087.

Gene expression databases

GenevisibleiO23087. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECA2_ARATH
AccessioniPrimary (citable) accession number: O23087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.