O23087 (ECA2_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 119.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Calcium-transporting ATPase 2, endoplasmic reticulum-type EC=3.6.3.8 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis![]() |
Protein attributes
| Sequence length | 1054 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment. |
| Catalytic activity | ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2). |
| Subcellular location | |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. [View classification] |
Ontologies
| Keywords | |
|---|---|
| Biological process | Calcium transport Ion transport Transport |
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | ATP-binding Calcium Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Hydrolase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Cellular_component | endoplasmic reticulum Inferred from direct assay PubMed 22923678. Source: TAIR integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activityInferred from sequence or structural similarity Ref.1PubMed 12805592. Source: TAIR metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1054 | 1054 | Calcium-transporting ATPase 2, endoplasmic reticulum-type | PRO_0000046406 | |||||
Regions | |||||||||
| Topological domain | 1 – 53 | 53 | Cytoplasmic Potential | ||||||
| Transmembrane | 54 – 74 | 21 | Helical; Potential | ||||||
| Topological domain | 75 – 98 | 24 | Lumenal Potential | ||||||
| Transmembrane | 99 – 118 | 20 | Helical; Potential | ||||||
| Topological domain | 119 – 262 | 144 | Cytoplasmic Potential | ||||||
| Transmembrane | 263 – 282 | 20 | Helical; Potential | ||||||
| Topological domain | 283 – 312 | 30 | Lumenal Potential | ||||||
| Transmembrane | 313 – 330 | 18 | Helical; Potential | ||||||
| Topological domain | 331 – 782 | 452 | Cytoplasmic Potential | ||||||
| Transmembrane | 783 – 802 | 20 | Helical; Potential | ||||||
| Topological domain | 803 – 812 | 10 | Lumenal Potential | ||||||
| Transmembrane | 813 – 833 | 21 | Helical; Potential | ||||||
| Topological domain | 834 – 853 | 20 | Cytoplasmic Potential | ||||||
| Transmembrane | 854 – 876 | 23 | Helical; Potential | ||||||
| Topological domain | 877 – 949 | 73 | Lumenal Potential | ||||||
| Transmembrane | 950 – 969 | 20 | Helical; Potential | ||||||
| Topological domain | 970 – 982 | 13 | Cytoplasmic Potential | ||||||
| Transmembrane | 983 – 1001 | 19 | Helical; Potential | ||||||
| Topological domain | 1002 – 1016 | 15 | Lumenal Potential | ||||||
| Transmembrane | 1017 – 1037 | 21 | Helical; Potential | ||||||
| Topological domain | 1038 – 1054 | 17 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Active site | 368 | 1 | 4-aspartylphosphate intermediate By similarity | ||||||
| Metal binding | 321 | 1 | Calcium 2; via carbonyl oxygen By similarity | ||||||
| Metal binding | 322 | 1 | Calcium 2; via carbonyl oxygen By similarity | ||||||
| Metal binding | 324 | 1 | Calcium 2; via carbonyl oxygen By similarity | ||||||
| Metal binding | 326 | 1 | Calcium 2 By similarity | ||||||
| Metal binding | 727 | 1 | Magnesium By similarity | ||||||
| Metal binding | 731 | 1 | Magnesium By similarity | ||||||
| Metal binding | 793 | 1 | Calcium 1 By similarity | ||||||
| Metal binding | 796 | 1 | Calcium 1 By similarity | ||||||
| Metal binding | 821 | 1 | Calcium 2 By similarity | ||||||
| Metal binding | 824 | 1 | Calcium 1 By similarity | ||||||
| Metal binding | 825 | 1 | Calcium 1 By similarity | ||||||
| Metal binding | 825 | 1 | Calcium 2 By similarity | ||||||
| Metal binding | 960 | 1 | Calcium 1 By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist." Pittman J.K., Mills R.F., O'Connor C.D., Williams L.E. Gene 236:137-147(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ132387 mRNA. Translation: CAA10659.1. AF013294 Genomic DNA. Translation: AAB62850.1. AL161472 Genomic DNA. Translation: CAB80899.1. CP002687 Genomic DNA. Translation: AEE81953.1. |
| IPI | IPI00524443. |
| PIR | T01556. |
| RefSeq | NP_191999.1. NM_116317.3. |
| UniGene | At.488. |
3D structure databases | |
| ProteinModelPortal | O23087. |
| SMR | O23087. Positions 28-388, 578-808. |
| ModBase | Search... |
Protein-protein interaction databases | |
| MINT | MINT-8065871. |
Proteomic databases | |
| PaxDb | O23087. |
| PRIDE | O23087. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G00900.1; AT4G00900.1; AT4G00900. |
| GeneID | 827984. |
| KEGG | ath:AT4G00900. |
Organism-specific databases | |
| TAIR | At4g00900. |
Phylogenomic databases | |
| eggNOG | COG0474. |
| HOGENOM | HOG000265621. |
| InParanoid | O23087. |
| KO | K01537. |
| OMA | VDGDPME. |
| PhylomeDB | O23087. |
| ProtClustDB | CLSN2686070. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MONOMER-14601. |
Gene expression databases | |
| ArrayExpress | O23087. |
| Genevestigator | O23087. |
| GermOnline | AT4G00900. Arabidopsis thaliana. |
Family and domain databases | |
| Gene3D | 1.20.1110.10. 3 hits. 2.70.150.10. 2 hits. 3.40.1110.10. 1 hit. |
| InterPro | IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view] |
| PANTHER | PTHR24093. PTHR24093. 1 hit. |
| Pfam | PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view] |
| PRINTS | PR00119. CATATPASE. |
| SMART | SM00831. Cation_ATPase_N. 1 hit. [Graphical view] |
| SUPFAM | SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit. |
| TIGRFAMs | TIGR01494. ATPase_P-type. 3 hits. |
| PROSITE | PS00154. ATPASE_E1_E2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ECA2_ARATH | ||||||||
| Accession | Primary (citable) accession number: O23087 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
