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Protein

Calcium-transporting ATPase 2, endoplasmic reticulum-type

Gene

ECA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi321 – 3211Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi322 – 3221Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi324 – 3241Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi326 – 3261Calcium 2By similarity
Active sitei368 – 36814-aspartylphosphate intermediateBy similarity
Metal bindingi727 – 7271MagnesiumBy similarity
Metal bindingi731 – 7311MagnesiumBy similarity
Metal bindingi793 – 7931Calcium 1By similarity
Metal bindingi796 – 7961Calcium 1By similarity
Metal bindingi821 – 8211Calcium 2By similarity
Metal bindingi824 – 8241Calcium 1By similarity
Metal bindingi825 – 8251Calcium 1By similarity
Metal bindingi825 – 8251Calcium 2By similarity
Metal bindingi960 – 9601Calcium 1By similarity

GO - Molecular functioni

GO - Biological processi

  • calcium ion transmembrane transport Source: GOC
  • calcium ion transport Source: TAIR
  • cation transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G00900-MONOMER.
MetaCyc:MONOMER-14601.
BRENDAi3.6.3.8. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 2, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:ECA2
Synonyms:ACA5
Ordered Locus Names:At4g00900
ORF Names:A_TM018A10.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G00900.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5353CytoplasmicSequence analysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence analysisAdd
BLAST
Topological domaini75 – 9824LumenalSequence analysisAdd
BLAST
Transmembranei99 – 11820HelicalSequence analysisAdd
BLAST
Topological domaini119 – 262144CytoplasmicSequence analysisAdd
BLAST
Transmembranei263 – 28220HelicalSequence analysisAdd
BLAST
Topological domaini283 – 31230LumenalSequence analysisAdd
BLAST
Transmembranei313 – 33018HelicalSequence analysisAdd
BLAST
Topological domaini331 – 782452CytoplasmicSequence analysisAdd
BLAST
Transmembranei783 – 80220HelicalSequence analysisAdd
BLAST
Topological domaini803 – 81210LumenalSequence analysis
Transmembranei813 – 83321HelicalSequence analysisAdd
BLAST
Topological domaini834 – 85320CytoplasmicSequence analysisAdd
BLAST
Transmembranei854 – 87623HelicalSequence analysisAdd
BLAST
Topological domaini877 – 94973LumenalSequence analysisAdd
BLAST
Transmembranei950 – 96920HelicalSequence analysisAdd
BLAST
Topological domaini970 – 98213CytoplasmicSequence analysisAdd
BLAST
Transmembranei983 – 100119HelicalSequence analysisAdd
BLAST
Topological domaini1002 – 101615LumenalSequence analysisAdd
BLAST
Transmembranei1017 – 103721HelicalSequence analysisAdd
BLAST
Topological domaini1038 – 105417CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054Calcium-transporting ATPase 2, endoplasmic reticulum-typePRO_0000046406Add
BLAST

Proteomic databases

PaxDbiO23087.
PRIDEiO23087.

PTM databases

SwissPalmiO23087.

Expressioni

Gene expression databases

GenevisibleiO23087. AT.

Interactioni

Protein-protein interaction databases

BioGridi13275. 4 interactions.
MINTiMINT-8065871.
STRINGi3702.AT4G00900.1.

Structurei

3D structure databases

ProteinModelPortaliO23087.
SMRiO23087. Positions 28-1041.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO23087.
KOiK01537.
OMAiPLMRNLN.
PhylomeDBiO23087.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O23087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEEKSFSAW SWSVEQCLKE YKTRLDKGLT SEDVQIRRQK YGFNELAKEK
60 70 80 90 100
GKPLWHLVLE QFDDTLVKIL LGAAFISFVL AFLGEEHGSG SGFEAFVEPF
110 120 130 140 150
VIVLILILNA VVGVWQESNA EKALEALKEM QCESAKVLRD GNVLPNLPAR
160 170 180 190 200
ELVPGDIVEL NVGDKVPADM RVSGLKTSTL RVEQSSLTGE AMPVLKGANL
210 220 230 240 250
VVMDDCELQG KENMVFAGTT VVNGSCVCIV TSIGMDTEIG KIQRQIHEAS
260 270 280 290 300
LEESETPLKK KLDEFGSRLT TAICIVCVLV WMINYKNFVS WDVVDGYKPV
310 320 330 340 350
NIKFSFEKCT YYFKIAVALA VAAIPEGLPA VITTCLALGT RKMAQKNAIV
360 370 380 390 400
RKLPSVETLG CTTVICSDKT GTLTTNQMSA TEFFTLGGKT TTTRVFSVSG
410 420 430 440 450
TTYDPKDGGI VDWGCNNMDA NLQAVAEICS ICNDAGVFYE GKLFRATGLP
460 470 480 490 500
TEAALKVLVE KMGIPEKKNS ENIEEVTNFS DNGSSVKLAC CDWWNKRSKK
510 520 530 540 550
VATLEFDRVR KSMSVIVSEP NGQNRLLVKG AAESILERSS FAQLADGSLV
560 570 580 590 600
ALDESSREVI LKKHSEMTSK GLRCLGLAYK DELGEFSDYS SEEHPSHKKL
610 620 630 640 650
LDPSSYSNIE TNLIFVGVVG LRDPPREEVG RAIEDCRDAG IRVMVITGDN
660 670 680 690 700
KSTAEAICCE IRLFSENEDL SQSSFTGKEF MSLPASRRSE ILSKSGGKVF
710 720 730 740 750
SRAEPRHKQE IVRMLKEMGE IVAMTGDGVN DAPALKLADI GIAMGITGTE
760 770 780 790 800
VAKEASDMVL ADDNFSTIVS AVAEGRSIYN NMKAFIRYMI SSNVGEVISI
810 820 830 840 850
FLTAALGIPE CMIPVQLLWV NLVTDGPPAT ALGFNPADID IMKKPPRKSD
860 870 880 890 900
DCLIDSWVLI RYLVIGSYVG VATVGIFVLW YTQASFLGIS LISDGHTLVS
910 920 930 940 950
FTQLQNWSEC SSWGTNFTAT PYTVAGGLRT IAFENNPCDY FTLGKVKPMT
960 970 980 990 1000
LSLTVLVAIE MFNSLNALSE DNSLLTMPPW RNPWLLVAMT VSFALHCVIL
1010 1020 1030 1040 1050
YVPFLANVFG IVPLSFREWF VVILVSFPVI LIDEALKFIG RCRRTRIKKK

IKTM
Length:1,054
Mass (Da):115,830
Last modified:January 1, 1998 - v1
Checksum:iB0D7F7237A3496AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132387 mRNA. Translation: CAA10659.1.
AF013294 Genomic DNA. Translation: AAB62850.1.
AL161472 Genomic DNA. Translation: CAB80899.1.
CP002687 Genomic DNA. Translation: AEE81953.1.
PIRiT01556.
RefSeqiNP_191999.1. NM_116317.3.
UniGeneiAt.488.

Genome annotation databases

EnsemblPlantsiAT4G00900.1; AT4G00900.1; AT4G00900.
GeneIDi827984.
GrameneiAT4G00900.1; AT4G00900.1; AT4G00900.
KEGGiath:AT4G00900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132387 mRNA. Translation: CAA10659.1.
AF013294 Genomic DNA. Translation: AAB62850.1.
AL161472 Genomic DNA. Translation: CAB80899.1.
CP002687 Genomic DNA. Translation: AEE81953.1.
PIRiT01556.
RefSeqiNP_191999.1. NM_116317.3.
UniGeneiAt.488.

3D structure databases

ProteinModelPortaliO23087.
SMRiO23087. Positions 28-1041.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13275. 4 interactions.
MINTiMINT-8065871.
STRINGi3702.AT4G00900.1.

PTM databases

SwissPalmiO23087.

Proteomic databases

PaxDbiO23087.
PRIDEiO23087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G00900.1; AT4G00900.1; AT4G00900.
GeneIDi827984.
GrameneiAT4G00900.1; AT4G00900.1; AT4G00900.
KEGGiath:AT4G00900.

Organism-specific databases

TAIRiAT4G00900.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiO23087.
KOiK01537.
OMAiPLMRNLN.
PhylomeDBiO23087.

Enzyme and pathway databases

BioCyciARA:AT4G00900-MONOMER.
MetaCyc:MONOMER-14601.
BRENDAi3.6.3.8. 399.

Miscellaneous databases

PROiO23087.

Gene expression databases

GenevisibleiO23087. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two additional type IIA Ca(2+)-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist."
    Pittman J.K., Mills R.F., O'Connor C.D., Williams L.E.
    Gene 236:137-147(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiECA2_ARATH
AccessioniPrimary (citable) accession number: O23087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: April 13, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.