Reviewed, UniProtKB/Swiss-Prot O23051 (KAO1_ARATH)

Names and origin

Protein names

Recommended name:
    Ent-kaurenoic acid oxidase 1
      Short name=AtKAO1
    EC=1.14.13.79
Alternative name(s):
    Cytochrome P450 88A3

Gene names

Name:	KAO1
Synonyms:	CYP88A3
Ordered Locus Names:	At1g05160
ORF Names:	YUP8H12.23

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes three successive oxidations of ent-kaurenoic acid giving gibberellin 12 (GA12), a key step in gibberellins (GAs) biosynthesis. GAs, which are involved many processes, including stem elongation, play a central role in plant development. Ref.1
Catalytic activity	Ent-kaur-16-en-19-oate + NADPH + O2 = ent-7-alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O. Ref.1 Ent-7-alpha-hydroxykaur-16-en-19-oate + NADPH + O2 = gibberellin A(12) aldehyde + NADP+ + 2 H2O. Ref.1 Gibberellin A(12) aldehyde + NADPH + O2 = gibberellin A(12) + NADP+ + H2O. Ref.1
Cofactor	Heme group By similarity.
Pathway	Plant hormone biosynthesis; gibberellin biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Ref.3
Tissue specificity	Widely expressed. Highly expressed in influorescence stem, influorescence, and silique tissue. Weakly expressed in cauline and rosette leaves. Expressed at a higher level in stem and influorescence than AtKAO2/CYP88A4. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gibberellin biosynthetic process Traceable author statement. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro ent-kaurenoate oxidase activity Ref.1 Inferred from direct assay. Source: TAIR heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Ent-kaurenoic acid oxidase 1		PRO_0000052178
Regions
	Transmembrane	6 – 26	21	Potential
Sites
	Metal binding	439	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O23051-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7FD8CD7A8864D298	FASTA	490	56,409
	10 20 30 40 50 60 MAETTSWIPV WFPLMVLGCF GLNWLVRKVN VWLYESSLGE NRHYLPPGDL GWPFIGNMLS 70 80 90 100 110 120 FLRAFKTSDP DSFTRTLIKR YGPKGIYKAH MFGNPSIIVT TSDTCRRVLT DDDAFKPGWP 130 140 150 160 170 180 TSTMELIGRK SFVGISFEEH KRLRRLTAAP VNGHEALSTY IPYIEENVIT VLDKWTKMGE 190 200 210 220 230 240 FEFLTHLRKL TFRIIMYIFL SSESENVMDA LEREYTALNY GVRAMAVNIP GFAYHRALKA 250 260 270 280 290 300 RKTLVAAFQS IVTERRNQRK QNILSNKKDM LDNLLNVKDE DGKTLDDEEI IDVLLMYLNA 310 320 330 340 350 360 GHESSGHTIM WATVFLQEHP EVLQRAKAEQ EMILKSRPEG QKGLSLKETR KMEFLSQVVD 370 380 390 400 410 420 ETLRVITFSL TAFREAKTDV EMNGYLIPKG WKVLTWFRDV HIDPEVFPDP RKFDPARWDN 430 440 450 460 470 480 GFVPKAGAFL PFGAGSHLCP GNDLAKLEIS IFLHHFLLKY QVKRSNPECP VMYLPHTRPT 490 DNCLARISYQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway." Helliwell C.A., Chandler P.M., Poole A., Dennis E.S., Peacock J.W. Proc. Natl. Acad. Sci. U.S.A. 98:2065-2070(2001) [PubMed: 11172076] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Silique.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"A plastid envelope location of Arabidopsis ent-kaurene oxidase links the plastid and endoplasmic reticulum steps of the gibberellin biosynthesis pathway." Helliwell C.A., Sullivan J.A., Mould R.M., Gray J.C., Peacock W.J., Dennis E.S. Plant J. 28:201-208(2001) [PubMed: 11722763] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF318500 mRNA. Translation: AAK11564.1. AC000098 Genomic DNA. Translation: AAB71462.1.
IPI	IPI00547281.
PIR	H86185.
RefSeq	NP_172008.1.
UniGene	At.10414
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Proteomic databases
PRIDE	O23051.
Genome annotation databases
GeneID	839311.
GenomeReviews	Gene locus AT1G05160 in contig CT485782_GR.
KEGG	ath:AT1G05160.
Organism-specific databases
GeneFarm	1616. 94.
TAIR	At1g05160.
Phylogenomic databases
OMA	O23051. ISNIVER.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G05160-MON.
BRENDA	1.14.13.79. 302.
Gene expression databases
ArrayExpress	O23051.
GermOnline	AT1G05160. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR002397. Cyt_P450_B. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00359. BP450. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAO1_ARATH

Accession

Primary (citable) accession number: O23051
Secondary accession number(s): Q9C5Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)