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O23044 (PER3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxidase 3
Short name=Atperox P3
EC=1.11.1.7
Alternative name(s):
ATPRC
RCI3A
Rare cold-inducible protein
Gene names
Name:PER3
Synonyms:P3, RCI3
Ordered Locus Names:At1g05260
ORF Names:YUP8H12.13
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Catalytic activity

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in root cells.

Induction

Up-regulated by cold temperatures and down-regulated by light. In response to low temperatures, transcripts accumulate in the whole etiolated seedlings but only in roots of light-grown seedlings.

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Sequence similarities

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 326302Peroxidase 3
PRO_0000023669

Sites

Active site661Proton acceptor By similarity
Metal binding671Calcium 1 By similarity
Metal binding701Calcium 1; via carbonyl oxygen By similarity
Metal binding721Calcium 1; via carbonyl oxygen By similarity
Metal binding741Calcium 1 By similarity
Metal binding761Calcium 1 By similarity
Metal binding1911Iron (heme axial ligand) By similarity
Metal binding1921Calcium 2 By similarity
Metal binding2441Calcium 2 By similarity
Metal binding2471Calcium 2 By similarity
Metal binding2521Calcium 2 By similarity
Binding site1611Substrate; via carbonyl oxygen By similarity
Site621Transition state stabilizer By similarity

Amino acid modifications

Modified residue251Pyrrolidone carboxylic acid By similarity
Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation2071N-linked (GlcNAc...) Potential
Glycosylation2371N-linked (GlcNAc...) Potential
Disulfide bond35 ↔ 113 By similarity
Disulfide bond68 ↔ 73 By similarity
Disulfide bond119 ↔ 321 By similarity
Disulfide bond198 ↔ 231 By similarity

Experimental info

Sequence conflict181I → M in AAM61240. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O23044 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 45D45362E5DD1B83

FASTA32634,906
        10         20         30         40         50         60 
MNCLIAIALS VSFFLVGIVG PIQAQLQMNF YANSCPNAEK IVQDFVSNHV SNAPSLAAAL 

        70         80         90        100        110        120 
IRMHFHDCFV RGCDGSVLIN STSGNAERDA TPNLTVRGFG FIDAIKSVLE AQCPGIVSCA 

       130        140        150        160        170        180 
DIIALASRDA VVFTGGPNWS VPTGRRDGRI SNAAEALANI PPPTSNITNL QTLFANQGLD 

       190        200        210        220        230        240 
LKDLVLLSGA HTIGVSHCSS FTNRLYNFTG RGGQDPALDS EYAANLKSRK CPSLNDNKTI 

       250        260        270        280        290        300 
VEMDPGSRKT FDLSYYQLVL KRRGLFQSDS ALTTNPTTLS NINRILTGSV GSFFSEFAKS 

       310        320 
MEKMGRINVK TGSAGVVRRQ CSVANS

« Hide

References

« Hide 'large scale' references
[1]"A novel cold-inducible gene from Arabidopsis, RCI3, encodes a peroxidase that constitutes a component for stress tolerance."
Llorente F., Lopez-Cobollo R.M., Catala R., Martinez-Zapater J.M., Salinas J.
Plant J. 32:13-24(2002) [PubMed: 12366797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Etiolated seedling.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
Tognolli M., Penel C., Greppin H., Simon P.
Gene 288:129-138(2002) [PubMed: 12034502] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U97684 mRNA. Translation: AAB94661.1.
AC000098 Genomic DNA. Translation: AAB71452.1.
CP002684 Genomic DNA. Translation: AEE27815.1.
BT004817 mRNA. Translation: AAO44083.1.
AY084678 mRNA. Translation: AAM61240.1.
IPIIPI00521040.
PIRB86187.
RefSeqNP_172018.1. NM_100405.3.
UniGeneAt.23986.

3D structure databases

ProteinModelPortalO23044.
SMRO23044. Positions 25-325.
ModBaseSearch...

Protein-protein interaction databases

IntActO23044. 1 interaction.
STRINGO23044.

Protein family/group databases

PeroxiBase79. AtPrx03.

Proteomic databases

PRIDEO23044.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G05260.1; AT1G05260.1; AT1G05260.
GeneID837028.
GenomeReviewsGene locus AT1G05260 in contig CT485782_GR.
KEGGath:AT1G05260.

Organism-specific databases

GeneFarm1833. 61.
TAIRAt1g05260.

Phylogenomic databases

HOGENOMHBG597790.
InParanoidO23044.
OMAANIPPPT.
PhylomeDBO23044.
ProtClustDBCLSN2681995.

Gene expression databases

ArrayExpressO23044.
GenevestigatorO23044.
GermOnlineAT1G05260. Arabidopsis thaliana.

Family and domain databases

InterProIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
KOK00430.
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMSSF48113. Peroxidase_super. 1 hit.
PROSITEPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePER3_ARATH
AccessionPrimary (citable) accession number: O23044
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: January 1, 1998
Last modified: December 14, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Recent format changes

Overview of recent format changes

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents