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Reviewed, UniProtKB/Swiss-Prot O23038 (PME8_ARATH)

Last modified October 13, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable pectinesterase 8
      Short name=PE 8
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 8
      Short name=AtPME8
    Pectin methylesterase 2
      Short name=AtPME2
Gene names
Name: PME8
Synonyms: ARATH2
Ordered Locus Names: At1g05310
ORF Names: YUP8H12.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall By similarity.

Tissue specificity

Expressed in siliques. Ref.3

Developmental stage

Expressed throughout silique development. Ref.3

Sequence similarities

Belongs to the pectinesterase family.

Sequence caution

The sequence AAB71446.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Secreted
   DomainSignal
   Molecular functionAspartyl esterase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionaspartyl esterase activity

Inferred from electronic annotation. Source: UniProtKB-KW

pectinesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 393374Probable pectinesterase 8
PRO_0000371665

Regions

Compositional bias47 – 6115His-rich

Sites

Active site2231Proton donor By similarity
Active site2441Nucleophile By similarity
Binding site1651Substrate By similarity
Binding site2001Substrate By similarity
Binding site3081Substrate By similarity
Site2221Transition state stabilizer By similarity

Amino acid modifications

Glycosylation1001N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1631N-linked (GlcNAc...) Potential
Glycosylation1821N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation3501N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation3781N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
O23038-1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 34362A9BE1B5263C

FASTA39343,350
        10         20         30         40         50         60 
MKIISLSISI GIAIIAVLAS KTLFKTHPEA FGIKAISYSF KKSLCDHHHH HHHHHHHHHR 

        70         80         90        100        110        120 
HKPSDTKRKV SICDDFPKNI PPLDTDTTSY LCVDKNGCCN FTTVQSAVDA VGNFSQRRNV 

       130        140        150        160        170        180 
IWINSGMYYE KVVIPKTKPN ITLQGQGFDI TAIAWNDTAY SANGTFYCAT VQVFGSQFVA 

       190        200        210        220        230        240 
KNISFMNVAP IPKPGDVGAQ AVAIRIAGDE SAFVGCGFFG AQDTLHDDRG RHYFKDCYIQ 

       250        260        270        280        290        300 
GSIDFIFGNA KSLYQDCRII SMANQLSPGS KAVNGAVTAN GRSSKDENSG FSFVNCTIGG 

       310        320        330        340        350        360 
TGHVWLGRAW RPYSRVVFVS TTMTDVIAPE GWNNFNDPSR DATIFYGEYN CSGPGADMSK 

       370        380        390 
RAPYVQKLNE TQVALLINTS FIDGDQWLQF SDL

« Hide

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References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Pectin methylesterases: sequence-structural features and phylogenetic relationships."
Markovic O., Janecek S.
Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[3]"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana."
Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J.
Planta 224:782-791(2006) [PubMed: 16622707] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AC000098 Genomic DNA. Translation: AAB71446.1. Sequence problems.
IPIIPI00525758.
PIRH86187.
RefSeqNP_172023.1.

3D structure databases

HSSPHSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ModBaseSearch...

Proteomic databases

PRIDEO23038.

Genome annotation databases

GeneID837030.
GenomeReviewsGene locus AT1G05310 in contig CT485782_GR.

Organism-specific databases

GeneFarm464. 8.
TAIRAt1g05310.

Gene expression databases

GenevestigatorO23038.

Family and domain databases

InterProIPR018040. Pectinesterase_AS.
[Graphical view]
PROSITEPS00800. PECTINESTERASE_1. False negative.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePME8_ARATH
AccessionPrimary (citable) accession number: O23038
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: October 13, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents