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Protein

CBL-interacting serine/threonine-protein kinase 13

Gene

CIPK13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861ATPPROSITE-ProRule annotation
Active sitei179 – 1791Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 719ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G34180-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 13 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.7
SOS2-like protein kinase PKS10
Gene namesi
Name:CIPK13
Synonyms:PKS10, SnRK3.7
Ordered Locus Names:At2g34180
ORF Names:F13P17.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G34180.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502CBL-interacting serine/threonine-protein kinase 13PRO_0000337215Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011PhosphoserineBy similarity
Modified residuei215 – 2151PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22971.
PRIDEiO22971.

Expressioni

Gene expression databases

GenevisibleiO22971. AT.

Interactioni

Subunit structurei

Interacts with CBL2 and CBL3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CBL2Q8LAS74EBI-637402,EBI-485991

Protein-protein interaction databases

BioGridi3326. 6 interactions.
IntActiO22971. 2 interactions.
STRINGi3702.AT2G34180.1.

Structurei

3D structure databases

ProteinModelPortaliO22971.
SMRiO22971. Positions 14-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 311255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini366 – 38722NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 22630Activation loopBy similarityAdd
BLAST
Regioni390 – 41930PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO22971.
OMAiSVRLEGC.
PhylomeDBiO22971.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVLSTPLA IPGPTPIQFM AGLLARIVTK NTNKETSTPE SPRSPRTPQG
60 70 80 90 100
SILMDKYEIG KLLGHGSFAK VYLARNIHSG EDVAIKVIDK EKIVKSGLAG
110 120 130 140 150
HIKREISILR RVRHPYIVHL LEVMATKTKI YIVMEYVRGG ELYNTVARGR
160 170 180 190 200
LREGTARRYF QQLISSVAFC HSRGVYHRDL KLENLLLDDK GNVKVSDFGL
210 220 230 240 250
SVVSEQLKQE GICQTFCGTP AYLAPEVLTR KGYEGAKADI WSCGVILFVL
260 270 280 290 300
MAGYLPFDDK NILVMYTKIY KGQFKCPKWF SPELARLVTR MLDTNPDTRI
310 320 330 340 350
TIPEIMKHRW FKKGFKHVKF YIENDKLCRE DDDNDDDDSS SLSSGRSSTA
360 370 380 390 400
SEGDAEFDIK RVDSMPRPAS LNAFDILSFS DLSGLFEEGG QGARFVSAAP
410 420 430 440 450
MTKIISKLEE IAKEVKFMVR KKDWSVRLEG CREGAKGPLT IRVEIFELTP
460 470 480 490 500
SLVVVEVKKK GGNIEEYEEF CNKELRPQLE KLMHYQADEV EEVMCLPPEI

EQ
Length:502
Mass (Da):56,731
Last modified:August 1, 1999 - v2
Checksum:iC74597664AAA3C09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295668 mRNA. Translation: AAK16688.1.
AC004481 Genomic DNA. Translation: AAC27394.1.
CP002685 Genomic DNA. Translation: AEC08929.1.
PIRiT02306.
RefSeqiNP_180965.1. NM_128969.1.
UniGeneiAt.43061.
At.64959.

Genome annotation databases

EnsemblPlantsiAT2G34180.1; AT2G34180.1; AT2G34180.
GeneIDi817979.
GrameneiAT2G34180.1; AT2G34180.1; AT2G34180.
KEGGiath:AT2G34180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295668 mRNA. Translation: AAK16688.1.
AC004481 Genomic DNA. Translation: AAC27394.1.
CP002685 Genomic DNA. Translation: AEC08929.1.
PIRiT02306.
RefSeqiNP_180965.1. NM_128969.1.
UniGeneiAt.43061.
At.64959.

3D structure databases

ProteinModelPortaliO22971.
SMRiO22971. Positions 14-484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3326. 6 interactions.
IntActiO22971. 2 interactions.
STRINGi3702.AT2G34180.1.

Proteomic databases

PaxDbiO22971.
PRIDEiO22971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G34180.1; AT2G34180.1; AT2G34180.
GeneIDi817979.
GrameneiAT2G34180.1; AT2G34180.1; AT2G34180.
KEGGiath:AT2G34180.

Organism-specific databases

TAIRiAT2G34180.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO22971.
OMAiSVRLEGC.
PhylomeDBiO22971.

Enzyme and pathway databases

BioCyciARA:AT2G34180-MONOMER.

Miscellaneous databases

PROiO22971.

Gene expression databases

GenevisibleiO22971. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The NAF domain defines a novel protein-protein interaction module conserved in Ca(2+)-regulated kinases."
    Albrecht V., Ritz O., Linder S., Harter K., Kudla J.
    EMBO J. 20:1051-1063(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH CBL2 AND CBL3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiCIPKD_ARATH
AccessioniPrimary (citable) accession number: O22971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: August 1, 1999
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.