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Protein

CBL-interacting serine/threonine-protein kinase 11

Gene

CIPK11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Acts as a negative regulator of the plasma membrane proton pump AHA2 by preventing its interaction with 14-3-3 protein.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+1 Publication, Mg2+1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501ATPPROSITE-ProRule annotation
Active sitei145 – 1451Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • protein phosphorylation Source: TAIR
  • response to pH Source: TAIR
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G30360-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 11 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.22
SOS2-like protein kinase PKS5
SOS3-interacting protein 4
Gene namesi
Name:CIPK11
Synonyms:PKS5, SIP4, SnRK3.22
Ordered Locus Names:At2g30360
ORF Names:T9D9.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30360.

Pathology & Biotechi

Disruption phenotypei

Increased proton extrusion and resistance to high external pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435CBL-interacting serine/threonine-protein kinase 11PRO_0000085875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671PhosphoserineBy similarity
Modified residuei181 – 1811PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22932.
PRIDEiO22932.

PTM databases

iPTMnetiO22932.

Expressioni

Tissue specificityi

Expressed in roots and stems, but barely detectable in flowers and siliques.2 Publications

Developmental stagei

Expressed in hypocotyls and roots upon germination. As seedlings mature, detected in the vascular tissue of both the stem and leaf.1 Publication

Inductioni

Up-regulated by salt, drought, abscisic acid and glucose, but not by cold.2 Publications

Gene expression databases

GenevisibleiO22932. AT.

Interactioni

Subunit structurei

Interacts with CBL1, CBL2, CBL3, CBL4/SOS3, and CBL5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AHA2P194563EBI-537638,EBI-2293350
CBL2Q8LAS75EBI-537638,EBI-485991

Protein-protein interaction databases

BioGridi2935. 32 interactions.
IntActiO22932. 8 interactions.
MINTiMINT-189416.
STRINGi3702.AT2G30360.1.

Structurei

3D structure databases

ProteinModelPortaliO22932.
SMRiO22932. Positions 19-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 277257Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini302 – 32625NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 19230Activation loopBy similarityAdd
BLAST
Regioni333 – 36230PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO22932.
OMAiIGICISR.
OrthoDBiEOG09360AXU.
PhylomeDBiO22932.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEIEIAAGS GDNNDALFGK YELGKLLGCG AFAKVFHARD RRTGQSVAVK
60 70 80 90 100
ILNKKKLLTN PALANNIKRE ISIMRRLSHP NIVKLHEVMA TKSKIFFAME
110 120 130 140 150
FVKGGELFNK ISKHGRLSED LSRRYFQQLI SAVGYCHARG VYHRDLKPEN
160 170 180 190 200
LLIDENGNLK VSDFGLSALT DQIRPDGLLH TLCGTPAYVA PEILSKKGYE
210 220 230 240 250
GAKVDVWSCG IVLFVLVAGY LPFNDPNVMN MYKKIYKGEY RFPRWMSPDL
260 270 280 290 300
KRFVSRLLDI NPETRITIDE ILKDPWFVRG GFKQIKFHDD EIEDQKVESS
310 320 330 340 350
LEAVKSLNAF DLISYSSGLD LSGLFAGCSN SSGESERFLS EKSPEMLAEE
360 370 380 390 400
VEGFAREENL RMKKKKEEEY GFEMEGQNGK FGIGICISRL NDLLVVVEAR
410 420 430
RRGGDGDCYK EMWNGKLRVQ LIRVCDQTSS TNAAI
Length:435
Mass (Da):49,017
Last modified:January 1, 1998 - v1
Checksum:i50C256B3C2B7D900
GO

Sequence cautioni

The sequence AAL16166 differs from that shown. Reason: Frameshift at position 343. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295666 mRNA. Translation: AAK16686.1.
AF339146 mRNA. Translation: AAK26844.1.
AC002338 Genomic DNA. Translation: AAC16938.1.
CP002685 Genomic DNA. Translation: AEC08375.1.
AF370595 mRNA. Translation: AAK43914.1.
AF428398 mRNA. Translation: AAL16166.1. Frameshift.
BT002478 mRNA. Translation: AAO00838.1.
BT006582 mRNA. Translation: AAP31926.1.
PIRiE84707.
RefSeqiNP_180595.1. NM_128589.2.
UniGeneiAt.12244.

Genome annotation databases

EnsemblPlantsiAT2G30360.1; AT2G30360.1; AT2G30360.
GeneIDi817586.
GrameneiAT2G30360.1; AT2G30360.1; AT2G30360.
KEGGiath:AT2G30360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295666 mRNA. Translation: AAK16686.1.
AF339146 mRNA. Translation: AAK26844.1.
AC002338 Genomic DNA. Translation: AAC16938.1.
CP002685 Genomic DNA. Translation: AEC08375.1.
AF370595 mRNA. Translation: AAK43914.1.
AF428398 mRNA. Translation: AAL16166.1. Frameshift.
BT002478 mRNA. Translation: AAO00838.1.
BT006582 mRNA. Translation: AAP31926.1.
PIRiE84707.
RefSeqiNP_180595.1. NM_128589.2.
UniGeneiAt.12244.

3D structure databases

ProteinModelPortaliO22932.
SMRiO22932. Positions 19-418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2935. 32 interactions.
IntActiO22932. 8 interactions.
MINTiMINT-189416.
STRINGi3702.AT2G30360.1.

PTM databases

iPTMnetiO22932.

Proteomic databases

PaxDbiO22932.
PRIDEiO22932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30360.1; AT2G30360.1; AT2G30360.
GeneIDi817586.
GrameneiAT2G30360.1; AT2G30360.1; AT2G30360.
KEGGiath:AT2G30360.

Organism-specific databases

TAIRiAT2G30360.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiO22932.
OMAiIGICISR.
OrthoDBiEOG09360AXU.
PhylomeDBiO22932.

Enzyme and pathway databases

BioCyciARA:AT2G30360-MONOMER.

Miscellaneous databases

PROiO22932.

Gene expression databases

GenevisibleiO22932. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPKB_ARATH
AccessioniPrimary (citable) accession number: O22932
Secondary accession number(s): Q7FYB7, Q944I6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.