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Protein

CBL-interacting serine/threonine-protein kinase 11

Gene

CIPK11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Acts as a negative regulator of the plasma membrane proton pump AHA2 by preventing its interaction with 14-3-3 protein.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+1 Publication, Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50ATPPROSITE-ProRule annotation1
Active sitei145Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 35ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • protein phosphorylation Source: TAIR
  • response to pH Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 11 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.22
SOS2-like protein kinase PKS5
SOS3-interacting protein 4
Gene namesi
Name:CIPK11
Synonyms:PKS5, SIP4, SnRK3.22
Ordered Locus Names:At2g30360
ORF Names:T9D9.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30360
TAIRilocus:2065781 AT2G30360

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Increased proton extrusion and resistance to high external pH.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858751 – 435CBL-interacting serine/threonine-protein kinase 11Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei167PhosphoserineBy similarity1
Modified residuei181PhosphothreonineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22932
PRIDEiO22932

PTM databases

iPTMnetiO22932

Expressioni

Tissue specificityi

Expressed in roots and stems, but barely detectable in flowers and siliques.2 Publications

Developmental stagei

Expressed in hypocotyls and roots upon germination. As seedlings mature, detected in the vascular tissue of both the stem and leaf.1 Publication

Inductioni

Up-regulated by salt, drought, abscisic acid and glucose, but not by cold.2 Publications

Gene expression databases

ExpressionAtlasiO22932 baseline and differential
GenevisibleiO22932 AT

Interactioni

Subunit structurei

Interacts with CBL1, CBL2, CBL3, CBL4/SOS3, and CBL5.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2935, 33 interactors
IntActiO22932, 8 interactors
STRINGi3702.AT2G30360.1

Structurei

3D structure databases

ProteinModelPortaliO22932
SMRiO22932
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST257
Domaini302 – 326NAFPROSITE-ProRule annotationAdd BLAST25

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 192Activation loopBy similarityAdd BLAST30
Regioni333 – 362PPIBy similarityAdd BLAST30

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0583 Eukaryota
COG0515 LUCA
HOGENOMiHOG000233016
InParanoidiO22932
OMAiIGICISR
OrthoDBiEOG09360AXU
PhylomeDBiO22932

Family and domain databases

InterProiView protein in InterPro
IPR020636 Ca/CaM-dep_Ca-dep_prot_Kinase
IPR011009 Kinase-like_dom_sf
IPR018451 NAF/FISL_domain
IPR004041 NAF_dom
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR43895 PTHR43895, 1 hit
PfamiView protein in Pfam
PF03822 NAF, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50816 NAF, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O22932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEIEIAAGS GDNNDALFGK YELGKLLGCG AFAKVFHARD RRTGQSVAVK
60 70 80 90 100
ILNKKKLLTN PALANNIKRE ISIMRRLSHP NIVKLHEVMA TKSKIFFAME
110 120 130 140 150
FVKGGELFNK ISKHGRLSED LSRRYFQQLI SAVGYCHARG VYHRDLKPEN
160 170 180 190 200
LLIDENGNLK VSDFGLSALT DQIRPDGLLH TLCGTPAYVA PEILSKKGYE
210 220 230 240 250
GAKVDVWSCG IVLFVLVAGY LPFNDPNVMN MYKKIYKGEY RFPRWMSPDL
260 270 280 290 300
KRFVSRLLDI NPETRITIDE ILKDPWFVRG GFKQIKFHDD EIEDQKVESS
310 320 330 340 350
LEAVKSLNAF DLISYSSGLD LSGLFAGCSN SSGESERFLS EKSPEMLAEE
360 370 380 390 400
VEGFAREENL RMKKKKEEEY GFEMEGQNGK FGIGICISRL NDLLVVVEAR
410 420 430
RRGGDGDCYK EMWNGKLRVQ LIRVCDQTSS TNAAI
Length:435
Mass (Da):49,017
Last modified:January 1, 1998 - v1
Checksum:i50C256B3C2B7D900
GO

Sequence cautioni

The sequence AAL16166 differs from that shown. Reason: Frameshift at position 343.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF295666 mRNA Translation: AAK16686.1
AF339146 mRNA Translation: AAK26844.1
AC002338 Genomic DNA Translation: AAC16938.1
CP002685 Genomic DNA Translation: AEC08375.1
AF370595 mRNA Translation: AAK43914.1
AF428398 mRNA Translation: AAL16166.1 Frameshift.
BT002478 mRNA Translation: AAO00838.1
BT006582 mRNA Translation: AAP31926.1
PIRiE84707
RefSeqiNP_180595.1, NM_128589.2
UniGeneiAt.12244

Genome annotation databases

EnsemblPlantsiAT2G30360.1; AT2G30360.1; AT2G30360
GeneIDi817586
GrameneiAT2G30360.1; AT2G30360.1; AT2G30360
KEGGiath:AT2G30360

Similar proteinsi

Entry informationi

Entry nameiCIPKB_ARATH
AccessioniPrimary (citable) accession number: O22932
Secondary accession number(s): Q7FYB7, Q944I6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health