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Protein

Vacuolar-sorting receptor 2

Gene

VSR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles.By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting receptor 2
Short name:
AtVSR2
Alternative name(s):
BP80-like protein c
Short name:
AtBP80c
Epidermal growth factor receptor-like protein 4
Short name:
AtELP4
Gene namesi
Name:VSR2
Synonyms:BP80C, ELP4
Ordered Locus Names:At2g30290
ORF Names:T9D9.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30290.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 567548LumenalSequence AnalysisAdd
BLAST
Transmembranei568 – 58821HelicalSequence AnalysisAdd
BLAST
Topological domaini589 – 62537CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. late endosome membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 625606Vacuolar-sorting receptor 2PRO_0000036464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi419 ↔ 437By similarity
Disulfide bondi426 ↔ 446By similarity
Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi448 ↔ 464By similarity
Disulfide bondi472 ↔ 492By similarity
Disulfide bondi479 ↔ 500By similarity
Disulfide bondi502 ↔ 514By similarity
Disulfide bondi544 ↔ 557By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO22925.
PRIDEiO22925.

Expressioni

Tissue specificityi

Expressed only in flowers.1 Publication

Gene expression databases

ExpressionAtlasiO22925. differential.
GenevestigatoriO22925.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G30290.1-P.

Structurei

3D structure databases

ProteinModelPortaliO22925.
SMRiO22925. Positions 438-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 167113PAAdd
BLAST
Domaini415 – 46551EGF-like 1Add
BLAST
Domaini468 – 51548EGF-like 2Add
BLAST
Domaini516 – 55843EGF-like 3; calcium-bindingSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi608 – 6114Tyrosine-based internalization motifBy similarity

Domaini

The tyrosine-based internalization signal may be involved in trafficking at the TGN.By similarity

Sequence similaritiesi

Belongs to the VSR (BP-80) family.Curated
Contains 3 EGF-like domains.Curated

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG302407.
HOGENOMiHOG000241019.
InParanoidiO22925.
PhylomeDBiO22925.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR003137. Protease-assoc_domain.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O22925-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTTNVWLVV IVWVTVGWSS CTGRFVVEKN NLRVTSPESI RGVYECALGN
60 70 80 90 100
FGVPQYGGSM SGAVVYPKTN QKACKNFDDF EISFRSRVAG LPTFVLVDRG
110 120 130 140 150
DCYFTLKAWN AQRAGAATIL VADNRPEQLI TMDAPEDETS DADYLQNITI
160 170 180 190 200
PSALVSRSLG SAIKTAIAHG DPVHISLDWR EALPHPNDRV AYELWTNSND
210 220 230 240 250
ECGSKCDAQI RFLKRFKGAA QILEKGGYTR FTPHYITWYC PEAFLASRQC
260 270 280 290 300
KTQCINGGRY CAPDPEQDFS RGYNGKDVII QNLRQACFFR VTNESGKPWL
310 320 330 340 350
WWDYVTDFAI RCPMKEEKYN KKCADQVIQS LGVDVKKIDK CIGDIDANAE
360 370 380 390 400
NPVLKEEQVA QVGKGSRGDV TILPTIVINN RQYRGKLQRS AVLKALCSGF
410 420 430 440 450
RETTEPPICL TEDIETNECL QNNGGCWEDK TTNITACRDT FRGRVCQCPI
460 470 480 490 500
VQGVKFLGDG YTHCEASGAL RCGINNGGCW KQTQMGKTYS ACRDDHSKGC
510 520 530 540 550
KCPPGFIGDG LKECKDVNEC EEKTACQCRD CKCKNTWGSY ECSCSGSLLY
560 570 580 590 600
IREHDICINR DARGDFSWGV IWIIIMGLGA AALGAYTVYK YRIRTYMDSE
610 620
IRAIMAQYMP LDNNPNTQLS SQLEL
Length:625
Mass (Da):69,875
Last modified:January 1, 1998 - v1
Checksum:i008F9E254ADDCE5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002338 Genomic DNA. Translation: AAC16948.1.
CP002685 Genomic DNA. Translation: AEC08366.1.
DQ446580 mRNA. Translation: ABE65874.1.
PIRiF84706.
RefSeqiNP_180588.1. NM_128582.1. [O22925-1]
UniGeneiAt.52975.

Genome annotation databases

EnsemblPlantsiAT2G30290.1; AT2G30290.1; AT2G30290. [O22925-1]
AT2G30290.2; AT2G30290.2; AT2G30290.
GeneIDi817579.
KEGGiath:AT2G30290.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002338 Genomic DNA. Translation: AAC16948.1.
CP002685 Genomic DNA. Translation: AEC08366.1.
DQ446580 mRNA. Translation: ABE65874.1.
PIRiF84706.
RefSeqiNP_180588.1. NM_128582.1. [O22925-1]
UniGeneiAt.52975.

3D structure databases

ProteinModelPortaliO22925.
SMRiO22925. Positions 438-560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G30290.1-P.

Proteomic databases

PaxDbiO22925.
PRIDEiO22925.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30290.1; AT2G30290.1; AT2G30290. [O22925-1]
AT2G30290.2; AT2G30290.2; AT2G30290.
GeneIDi817579.
KEGGiath:AT2G30290.

Organism-specific databases

TAIRiAT2G30290.

Phylogenomic databases

eggNOGiNOG302407.
HOGENOMiHOG000241019.
InParanoidiO22925.
PhylomeDBiO22925.

Gene expression databases

ExpressionAtlasiO22925. differential.
GenevestigatoriO22925.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR003137. Protease-assoc_domain.
[Graphical view]
PfamiPF07645. EGF_CA. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
    Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
    Plant Biotechnol. J. 4:317-324(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants."
    Laval V., Masclaux F., Serin A., Carriere M., Roldan C., Devic M., Pont-Lezica R.F., Galaud J.-P.
    J. Exp. Bot. 54:213-221(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana."
    Shimada T., Fuji K., Tamura K., Kondo M., Nishimura M., Hara-Nishimura I.
    Proc. Natl. Acad. Sci. U.S.A. 100:16095-16100(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiVSR2_ARATH
AccessioniPrimary (citable) accession number: O22925
Secondary accession number(s): Q1PEY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.