O22925 (VSR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar-sorting receptor 2
Short name=AtVSR2

Alternative name(s):
BP80-like protein c
Short name=AtBP80c
Epidermal growth factor receptor-like protein 4
Short name=AtELP4

Gene names

Name:	VSR2
Synonyms:	BP80C, ELP4
Ordered Locus Names:	At2g30290
ORF Names:	T9D9.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Single-pass type I membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type I membrane protein By similarity.
Tissue specificity	Expressed only in flowers. Ref.4
Domain	The tyrosine-based internalization signal may be involved in trafficking at the TGN By similarity.
Sequence similarities	Belongs to the VSR (BP-80) family. Contains 3 EGF-like domains. Contains 1 PA (protease associated) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O22925-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 625	606	Vacuolar-sorting receptor 2		PRO_0000036464
Regions
	Topological domain	20 – 567	548	Lumenal Potential
	Transmembrane	568 – 588	21	Helical; Potential
	Topological domain	589 – 625	37	Cytoplasmic Potential
	Domain	55 – 167	113	PA
	Domain	415 – 465	51	EGF-like 1
	Domain	468 – 515	48	EGF-like 2
	Domain	516 – 558	43	EGF-like 3; calcium-binding Potential
	Motif	608 – 611	4	Tyrosine-based internalization motif By similarity
Amino acid modifications
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Disulfide bond	419 ↔ 437		By similarity
	Disulfide bond	426 ↔ 446		By similarity
	Disulfide bond	448 ↔ 464		By similarity
	Disulfide bond	472 ↔ 492		By similarity
	Disulfide bond	479 ↔ 500		By similarity
	Disulfide bond	502 ↔ 514		By similarity
	Disulfide bond	544 ↔ 557		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 008F9E254ADDCE5C	FASTA	625	69,875
	10 20 30 40 50 60 MRTTNVWLVV IVWVTVGWSS CTGRFVVEKN NLRVTSPESI RGVYECALGN FGVPQYGGSM 70 80 90 100 110 120 SGAVVYPKTN QKACKNFDDF EISFRSRVAG LPTFVLVDRG DCYFTLKAWN AQRAGAATIL 130 140 150 160 170 180 VADNRPEQLI TMDAPEDETS DADYLQNITI PSALVSRSLG SAIKTAIAHG DPVHISLDWR 190 200 210 220 230 240 EALPHPNDRV AYELWTNSND ECGSKCDAQI RFLKRFKGAA QILEKGGYTR FTPHYITWYC 250 260 270 280 290 300 PEAFLASRQC KTQCINGGRY CAPDPEQDFS RGYNGKDVII QNLRQACFFR VTNESGKPWL 310 320 330 340 350 360 WWDYVTDFAI RCPMKEEKYN KKCADQVIQS LGVDVKKIDK CIGDIDANAE NPVLKEEQVA 370 380 390 400 410 420 QVGKGSRGDV TILPTIVINN RQYRGKLQRS AVLKALCSGF RETTEPPICL TEDIETNECL 430 440 450 460 470 480 QNNGGCWEDK TTNITACRDT FRGRVCQCPI VQGVKFLGDG YTHCEASGAL RCGINNGGCW 490 500 510 520 530 540 KQTQMGKTYS ACRDDHSKGC KCPPGFIGDG LKECKDVNEC EEKTACQCRD CKCKNTWGSY 550 560 570 580 590 600 ECSCSGSLLY IREHDICINR DARGDFSWGV IWIIIMGLGA AALGAYTVYK YRIRTYMDSE 610 620 IRAIMAQYMP LDNNPNTQLS SQLEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants." Laval V., Masclaux F., Serin A., Carriere M., Roldan C., Devic M., Pont-Lezica R.F., Galaud J.-P. J. Exp. Bot. 54:213-221(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana." Shimada T., Fuji K., Tamura K., Kondo M., Nishimura M., Hara-Nishimura I. Proc. Natl. Acad. Sci. U.S.A. 100:16095-16100(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002338 Genomic DNA. Translation: AAC16948.1. CP002685 Genomic DNA. Translation: AEC08366.1. DQ446580 mRNA. Translation: ABE65874.1.
IPI	IPI00520341.
PIR	F84706.
RefSeq	NP_180588.1. NM_128582.1.
UniGene	At.52975.
3D structure databases
ProteinModelPortal	O22925.
SMR	O22925. Positions 393-518.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G30290.1-P.
Proteomic databases
PaxDb	O22925.
PRIDE	O22925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30290.1; AT2G30290.1; AT2G30290.
GeneID	817579.
KEGG	ath:AT2G30290.
Organism-specific databases
TAIR	At2g30290.
Phylogenomic databases
eggNOG	NOG302407.
HOGENOM	HOG000241019.
InParanoid	O22925.
PhylomeDB	O22925.
ProtClustDB	CLSN2682336.
Gene expression databases
ArrayExpress	O22925.
Genevestigator	O22925.
Family and domain databases
InterPro	IPR001881. EGF-like_Ca-bd. IPR018097. EGF_Ca-bd_CS. IPR003137. Protease-assoc_domain. [Graphical view]
Pfam	PF07645. EGF_CA. 1 hit. PF02225. PA. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VSR2_ARATH

Accession

Primary (citable) accession number: O22925
Secondary accession number(s): Q1PEY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program