Reviewed, UniProtKB/Swiss-Prot O22925 (VSR2_ARATH)

Names and origin

Protein names

Recommended name:
    Vacuolar-sorting receptor 2
      Short name=AtVSR2
Alternative name(s):
    Epidermal growth factor receptor-like protein 4
      Short name=AtELP4
    BP80-like protein c
      Short name=AtBP80c

Gene names

Name:	VSR2
Synonyms:	BP80C, ELP4
Ordered Locus Names:	At2g30290
ORF Names:	T9D9.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Single-pass type I membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type I membrane protein By similarity.
Tissue specificity	Expressed only in flowers. Ref.3
Domain	The tyrosine-based internalization signal may be involved in trafficking at the TGN By similarity.
Sequence similarities	Belongs to the VSR (BP-80) family. Contains 3 EGF-like domains. Contains 1 PA (protease associated) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Domain	EGF-like domain Repeat Signal Transmembrane
Ligand	Calcium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 625	606	Vacuolar-sorting receptor 2		PRO_0000036464
Regions
	Topological domain	20 – 567	548	Lumenal Potential
	Transmembrane	568 – 588	21	Potential
	Topological domain	589 – 625	37	Cytoplasmic Potential
	Domain	55 – 167	113	PA
	Domain	415 – 465	51	EGF-like 1
	Domain	468 – 515	48	EGF-like 2
	Domain	516 – 558	43	EGF-like 3; calcium-binding Potential
	Motif	608 – 611	4	Tyrosine-based internalization motif By similarity
Amino acid modifications
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Disulfide bond	419 ↔ 437		By similarity
	Disulfide bond	426 ↔ 446		By similarity
	Disulfide bond	448 ↔ 464		By similarity
	Disulfide bond	472 ↔ 492		By similarity
	Disulfide bond	479 ↔ 500		By similarity
	Disulfide bond	502 ↔ 514		By similarity
	Disulfide bond	544 ↔ 557		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22925-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 008F9E254ADDCE5C	FASTA	625	69,875
	10 20 30 40 50 60 MRTTNVWLVV IVWVTVGWSS CTGRFVVEKN NLRVTSPESI RGVYECALGN FGVPQYGGSM 70 80 90 100 110 120 SGAVVYPKTN QKACKNFDDF EISFRSRVAG LPTFVLVDRG DCYFTLKAWN AQRAGAATIL 130 140 150 160 170 180 VADNRPEQLI TMDAPEDETS DADYLQNITI PSALVSRSLG SAIKTAIAHG DPVHISLDWR 190 200 210 220 230 240 EALPHPNDRV AYELWTNSND ECGSKCDAQI RFLKRFKGAA QILEKGGYTR FTPHYITWYC 250 260 270 280 290 300 PEAFLASRQC KTQCINGGRY CAPDPEQDFS RGYNGKDVII QNLRQACFFR VTNESGKPWL 310 320 330 340 350 360 WWDYVTDFAI RCPMKEEKYN KKCADQVIQS LGVDVKKIDK CIGDIDANAE NPVLKEEQVA 370 380 390 400 410 420 QVGKGSRGDV TILPTIVINN RQYRGKLQRS AVLKALCSGF RETTEPPICL TEDIETNECL 430 440 450 460 470 480 QNNGGCWEDK TTNITACRDT FRGRVCQCPI VQGVKFLGDG YTHCEASGAL RCGINNGGCW 490 500 510 520 530 540 KQTQMGKTYS ACRDDHSKGC KCPPGFIGDG LKECKDVNEC EEKTACQCRD CKCKNTWGSY 550 560 570 580 590 600 ECSCSGSLLY IREHDICINR DARGDFSWGV IWIIIMGLGA AALGAYTVYK YRIRTYMDSE 610 620 IRAIMAQYMP LDNNPNTQLS SQLEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed: 17147637] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants." Laval V., Masclaux F., Serin A., Carriere M., Roldan C., Devic M., Pont-Lezica R.F., Galaud J.-P. J. Exp. Bot. 54:213-221(2003) [PubMed: 12493849] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana." Shimada T., Fuji K., Tamura K., Kondo M., Nishimura M., Hara-Nishimura I. Proc. Natl. Acad. Sci. U.S.A. 100:16095-16100(2003) [PubMed: 14657332] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002338 Genomic DNA. Translation: AAC16948.1. DQ446580 mRNA. Translation: ABE65874.1.
IPI	IPI00520341.
PIR	F84706.
RefSeq	NP_180588.1.
UniGene	At.52975
3D structure databases
SMR	O22925. Positions 497-549.
ModBase	Search...
Proteomic databases
PRIDE	O22925.
Genome annotation databases
GeneID	817579.
GenomeReviews	Gene locus AT2G30290 in contig CT485783_GR.
KEGG	ath:AT2G30290.
NMPDR	fig|3702.1.peg.10081.
Organism-specific databases
TAIR	At2g30290.
Phylogenomic databases
eggNOG	NOG136527.
HOGENOM	HBG319221.
InParanoid	O22925.
Gene expression databases
ArrayExpress	O22925.
Genevestigator	O22925.
Family and domain databases
InterPro	IPR013091. EGF_Ca_bd_2. IPR018097. EGF_Ca_bd_CS. IPR009030. Growth_fac_rcpt. IPR003137. PA. [Graphical view]
Pfam	PF07645. EGF_CA. 1 hit. PF02225. PA. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VSR2_ARATH

Accession

Primary (citable) accession number: O22925
Secondary accession number(s): Q1PEY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)