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O22925 (VSR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Vacuolar-sorting receptor 2

Short name=AtVSR2
Alternative name(s):
BP80-like protein c
Short name=AtBP80c
Epidermal growth factor receptor-like protein 4
Short name=AtELP4
Gene names
Name:VSR2
Synonyms:BP80C, ELP4
Ordered Locus Names:At2g30290
ORF Names:T9D9.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length625 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Vacuolar-sorting receptor (VSR) involved in clathrin-coated vesicles sorting from Golgi apparatus to vacuoles By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity. Golgi apparatus membrane; Single-pass type I membrane protein By similarity. Cytoplasmic vesicleclathrin-coated vesicle membrane; Single-pass type I membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Expressed only in flowers. Ref.4

Domain

The tyrosine-based internalization signal may be involved in trafficking at the TGN By similarity.

Sequence similarities

Belongs to the VSR (BP-80) family.

Contains 3 EGF-like domains.

Contains 1 PA (protease associated) domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O22925-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 625606Vacuolar-sorting receptor 2
PRO_0000036464

Regions

Topological domain20 – 567548Lumenal Potential
Transmembrane568 – 58821Helical; Potential
Topological domain589 – 62537Cytoplasmic Potential
Domain55 – 167113PA
Domain415 – 46551EGF-like 1
Domain468 – 51548EGF-like 2
Domain516 – 55843EGF-like 3; calcium-binding Potential
Motif608 – 6114Tyrosine-based internalization motif By similarity

Amino acid modifications

Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Disulfide bond419 ↔ 437 By similarity
Disulfide bond426 ↔ 446 By similarity
Disulfide bond448 ↔ 464 By similarity
Disulfide bond472 ↔ 492 By similarity
Disulfide bond479 ↔ 500 By similarity
Disulfide bond502 ↔ 514 By similarity
Disulfide bond544 ↔ 557 By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 008F9E254ADDCE5C

FASTA62569,875
        10         20         30         40         50         60 
MRTTNVWLVV IVWVTVGWSS CTGRFVVEKN NLRVTSPESI RGVYECALGN FGVPQYGGSM 

        70         80         90        100        110        120 
SGAVVYPKTN QKACKNFDDF EISFRSRVAG LPTFVLVDRG DCYFTLKAWN AQRAGAATIL 

       130        140        150        160        170        180 
VADNRPEQLI TMDAPEDETS DADYLQNITI PSALVSRSLG SAIKTAIAHG DPVHISLDWR 

       190        200        210        220        230        240 
EALPHPNDRV AYELWTNSND ECGSKCDAQI RFLKRFKGAA QILEKGGYTR FTPHYITWYC 

       250        260        270        280        290        300 
PEAFLASRQC KTQCINGGRY CAPDPEQDFS RGYNGKDVII QNLRQACFFR VTNESGKPWL 

       310        320        330        340        350        360 
WWDYVTDFAI RCPMKEEKYN KKCADQVIQS LGVDVKKIDK CIGDIDANAE NPVLKEEQVA 

       370        380        390        400        410        420 
QVGKGSRGDV TILPTIVINN RQYRGKLQRS AVLKALCSGF RETTEPPICL TEDIETNECL 

       430        440        450        460        470        480 
QNNGGCWEDK TTNITACRDT FRGRVCQCPI VQGVKFLGDG YTHCEASGAL RCGINNGGCW 

       490        500        510        520        530        540 
KQTQMGKTYS ACRDDHSKGC KCPPGFIGDG LKECKDVNEC EEKTACQCRD CKCKNTWGSY 

       550        560        570        580        590        600 
ECSCSGSLLY IREHDICINR DARGDFSWGV IWIIIMGLGA AALGAYTVYK YRIRTYMDSE 

       610        620 
IRAIMAQYMP LDNNPNTQLS SQLEL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations."
Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.
Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants."
Laval V., Masclaux F., Serin A., Carriere M., Roldan C., Devic M., Pont-Lezica R.F., Galaud J.-P.
J. Exp. Bot. 54:213-221(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana."
Shimada T., Fuji K., Tamura K., Kondo M., Nishimura M., Hara-Nishimura I.
Proc. Natl. Acad. Sci. U.S.A. 100:16095-16100(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002338 Genomic DNA. Translation: AAC16948.1.
CP002685 Genomic DNA. Translation: AEC08366.1.
DQ446580 mRNA. Translation: ABE65874.1.
PIRF84706.
RefSeqNP_180588.1. NM_128582.1. [O22925-1]
UniGeneAt.52975.

3D structure databases

ProteinModelPortalO22925.
SMRO22925. Positions 393-549.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G30290.1-P.

Proteomic databases

PaxDbO22925.
PRIDEO22925.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30290.1; AT2G30290.1; AT2G30290. [O22925-1]
AT2G30290.2; AT2G30290.2; AT2G30290.
GeneID817579.
KEGGath:AT2G30290.

Organism-specific databases

TAIRAT2G30290.

Phylogenomic databases

eggNOGNOG302407.
HOGENOMHOG000241019.
InParanoidO22925.
PhylomeDBO22925.

Gene expression databases

ArrayExpressO22925.
GenevestigatorO22925.

Family and domain databases

InterProIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR003137. Protease-assoc_domain.
[Graphical view]
PfamPF07645. EGF_CA. 1 hit.
PF02225. PA. 1 hit.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVSR2_ARATH
AccessionPrimary (citable) accession number: O22925
Secondary accession number(s): Q1PEY7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names