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Protein

Probable WRKY transcription factor 25

Gene

WRKY25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Functions with WRKY33 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Plays a partial role in heat stress tolerance (PubMed:19125253). Functions with WRKY26 and WRKY33 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597).By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi160 – 22465WRKY 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi322 – 38766WRKY 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. cellular heat acclimation Source: TAIR
  2. cellular response to heat Source: TAIR
  3. response to cold Source: TAIR
  4. response to osmotic stress Source: TAIR
  5. response to salt stress Source: TAIR
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable WRKY transcription factor 25
Alternative name(s):
WRKY DNA-binding protein 25
Gene namesi
Name:WRKY25
Ordered Locus Names:At2g30250
ORF Names:T9D9.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30250.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Probable WRKY transcription factor 25PRO_0000133667Add
BLAST

Post-translational modificationi

Phosphorylated by MPK4.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO22921.

Expressioni

Tissue specificityi

Highly expressed in roots and at lower levels in leaves, stems and seeds.1 Publication

Inductioni

By salt stress (PubMed:18839316). Induced by heat stress (PubMed:19125253).2 Publications

Gene expression databases

GenevestigatoriO22921.

Interactioni

Subunit structurei

Interacts with MKS1 (PubMed:15990873). Interacts with SIB1 (PubMed:21990940). Interacts with VQ10 and CAMBP25/VQ15 (PubMed:22535423).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MKS1Q8LGD53EBI-1392386,EBI-1392198
MPK4Q390242EBI-1392386,EBI-994375

Protein-protein interaction databases

BioGridi2924. 18 interactions.
IntActiO22921. 3 interactions.
MINTiMINT-8068882.
STRINGi3702.AT2G30250.1-P.

Structurei

3D structure databases

ProteinModelPortaliO22921.
SMRiO22921. Positions 166-224, 326-387.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the WRKY group I family.Curated
Contains 2 WRKY DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG302838.
HOGENOMiHOG000242409.
InParanoidiO22921.
KOiK13423.
OMAiEGMSIEV.
PhylomeDBiO22921.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_DNA-bd.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTSFTDLL GSSGVDCYED DEDLRVSGSS FGGYYPERTG SGLPKFKTAQ
60 70 80 90 100
PPPLPISQSS HNFTFSDYLD SPLLLSSSHS LISPTTGTFP LQGFNGTTNN
110 120 130 140 150
HSDFPWQLQS QPSNASSALQ ETYGVQDHEK KQEMIPNEIA TQNNNQSFGT
160 170 180 190 200
ERQIKIPAYM VSRNSNDGYG WRKYGQKQVK KSENPRSYFK CTYPDCVSKK
210 220 230 240 250
IVETASDGQI TEIIYKGGHN HPKPEFTKRP SQSSLPSSVN GRRLFNPASV
260 270 280 290 300
VSEPHDQSEN SSISFDYSDL EQKSFKSEYG EIDEEEEQPE MKRMKREGED
310 320 330 340 350
EGMSIEVSKG VKEPRVVVQT ISDIDVLIDG FRWRKYGQKV VKGNTNPRSY
360 370 380 390
YKCTFQGCGV KKQVERSAAD ERAVLTTYEG RHNHDIPTAL RRS
Length:393
Mass (Da):44,134
Last modified:January 1, 1998 - v1
Checksum:i9057D65B69E12A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418309 mRNA. Translation: AAL13040.1.
AC002338 Genomic DNA. Translation: AAC16930.1.
AC004165 Genomic DNA. Translation: AAM14918.1.
CP002685 Genomic DNA. Translation: AEC08362.1.
AY062720 mRNA. Translation: AAL32798.1.
AY114650 mRNA. Translation: AAM47969.1.
AY136318 mRNA. Translation: AAM96984.1.
AY045813 mRNA. Translation: AAK76487.2.
BT002338 mRNA. Translation: AAN86171.1.
BT008482 mRNA. Translation: AAP37841.1.
PIRiT00575.
RefSeqiNP_180584.1. NM_128578.3.
UniGeneiAt.12235.

Genome annotation databases

EnsemblPlantsiAT2G30250.1; AT2G30250.1; AT2G30250.
GeneIDi817575.
KEGGiath:AT2G30250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418309 mRNA. Translation: AAL13040.1.
AC002338 Genomic DNA. Translation: AAC16930.1.
AC004165 Genomic DNA. Translation: AAM14918.1.
CP002685 Genomic DNA. Translation: AEC08362.1.
AY062720 mRNA. Translation: AAL32798.1.
AY114650 mRNA. Translation: AAM47969.1.
AY136318 mRNA. Translation: AAM96984.1.
AY045813 mRNA. Translation: AAK76487.2.
BT002338 mRNA. Translation: AAN86171.1.
BT008482 mRNA. Translation: AAP37841.1.
PIRiT00575.
RefSeqiNP_180584.1. NM_128578.3.
UniGeneiAt.12235.

3D structure databases

ProteinModelPortaliO22921.
SMRiO22921. Positions 166-224, 326-387.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2924. 18 interactions.
IntActiO22921. 3 interactions.
MINTiMINT-8068882.
STRINGi3702.AT2G30250.1-P.

Proteomic databases

PRIDEiO22921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30250.1; AT2G30250.1; AT2G30250.
GeneIDi817575.
KEGGiath:AT2G30250.

Organism-specific databases

TAIRiAT2G30250.

Phylogenomic databases

eggNOGiNOG302838.
HOGENOMiHOG000242409.
InParanoidiO22921.
KOiK13423.
OMAiEGMSIEV.
PhylomeDBiO22921.

Gene expression databases

GenevestigatoriO22921.

Family and domain databases

Gene3Di2.20.25.80. 2 hits.
InterProiIPR003657. WRKY_DNA-bd.
[Graphical view]
PfamiPF03106. WRKY. 2 hits.
[Graphical view]
SMARTiSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMiSSF118290. SSF118290. 2 hits.
PROSITEiPS50811. WRKY. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis thaliana transcription factor WRKY25."
    Ulker B., Kushnir S., Somssich I.E.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Flower.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: INTERACTION WITH MKS1, PHOSPHORYLATION.
  6. "Functional analysis of an Arabidopsis transcription factor WRKY25 in heat stress."
    Li S., Fu Q., Huang W., Yu D.
    Plant Cell Rep. 28:683-693(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY HEAT.
  7. "Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses."
    Jiang Y., Deyholos M.K.
    Plant Mol. Biol. 69:91-105(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY SALT, DISRUPTION PHENOTYPE.
  8. "Arabidopsis thaliana WRKY25, WRKY26, and WRKY33 coordinate induction of plant thermotolerance."
    Li S., Fu Q., Chen L., Huang W., Yu D.
    Planta 233:1237-1252(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Arabidopsis sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense."
    Lai Z., Li Y., Wang F., Cheng Y., Fan B., Yu J.Q., Chen Z.
    Plant Cell 23:3824-3841(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SIB1.
  10. "Structural and functional analysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors."
    Cheng Y., Zhou Y., Yang Y., Chi Y.J., Zhou J., Chen J.Y., Wang F., Fan B., Shi K., Zhou Y.H., Yu J.Q., Chen Z.
    Plant Physiol. 159:810-825(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH VQ10 AND CAMBP25/VQ15.

Entry informationi

Entry nameiWRK25_ARATH
AccessioniPrimary (citable) accession number: O22921
Secondary accession number(s): Q94AT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 1, 1998
Last modified: March 4, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.