Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable WRKY transcription factor 25

Gene

WRKY25

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity). Functions with WRKY33 as positive regulator of salt stress response and abscisic acid (ABA) signaling (PubMed:18839316). Plays a partial role in heat stress tolerance (PubMed:19125253). Functions with WRKY26 and WRKY33 as positive regulator of plant thermotolerance by partially participating in ethylene-response signal transduction pathway (PubMed:21336597).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi160 – 224WRKY 1PROSITE-ProRule annotationAdd BLAST65
DNA bindingi322 – 387WRKY 2PROSITE-ProRule annotationAdd BLAST66

GO - Molecular functioni

  • DNA binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • cellular heat acclimation Source: TAIR
  • cellular response to heat Source: TAIR
  • response to cold Source: TAIR
  • response to osmotic stress Source: TAIR
  • response to salt stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable WRKY transcription factor 25
Alternative name(s):
WRKY DNA-binding protein 25
Gene namesi
Name:WRKY25
Ordered Locus Names:At2g30250
ORF Names:T9D9.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30250
TAIRilocus:2060805 AT2G30250

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001336671 – 393Probable WRKY transcription factor 25Add BLAST393

Post-translational modificationi

Phosphorylated by MPK4.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22921
PRIDEiO22921

Expressioni

Tissue specificityi

Highly expressed in roots and at lower levels in leaves, stems and seeds.1 Publication

Inductioni

By salt stress (PubMed:18839316). Induced by heat stress (PubMed:19125253).2 Publications

Gene expression databases

ExpressionAtlasiO22921 baseline and differential
GenevisibleiO22921 AT

Interactioni

Subunit structurei

Interacts with MKS1 (PubMed:15990873). Interacts with SIB1 (PubMed:21990940). Interacts with VQ10 and CAMBP25/VQ15 (PubMed:22535423).3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi2924, 19 interactors
IntActiO22921, 3 interactors
STRINGi3702.AT2G30250.1

Structurei

3D structure databases

ProteinModelPortaliO22921
SMRiO22921
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the WRKY group I family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410J384 Eukaryota
ENOG4112BTF LUCA
HOGENOMiHOG000242409
InParanoidiO22921
KOiK13423
OMAiSFRSEYG
OrthoDBiEOG09360CHC
PhylomeDBiO22921

Family and domain databases

Gene3Di2.20.25.80, 2 hits
InterProiView protein in InterPro
IPR003657 WRKY_dom
IPR036576 WRKY_dom_sf
PfamiView protein in Pfam
PF03106 WRKY, 2 hits
SMARTiView protein in SMART
SM00774 WRKY, 2 hits
SUPFAMiSSF118290 SSF118290, 2 hits
PROSITEiView protein in PROSITE
PS50811 WRKY, 2 hits

Sequencei

Sequence statusi: Complete.

O22921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTSFTDLL GSSGVDCYED DEDLRVSGSS FGGYYPERTG SGLPKFKTAQ
60 70 80 90 100
PPPLPISQSS HNFTFSDYLD SPLLLSSSHS LISPTTGTFP LQGFNGTTNN
110 120 130 140 150
HSDFPWQLQS QPSNASSALQ ETYGVQDHEK KQEMIPNEIA TQNNNQSFGT
160 170 180 190 200
ERQIKIPAYM VSRNSNDGYG WRKYGQKQVK KSENPRSYFK CTYPDCVSKK
210 220 230 240 250
IVETASDGQI TEIIYKGGHN HPKPEFTKRP SQSSLPSSVN GRRLFNPASV
260 270 280 290 300
VSEPHDQSEN SSISFDYSDL EQKSFKSEYG EIDEEEEQPE MKRMKREGED
310 320 330 340 350
EGMSIEVSKG VKEPRVVVQT ISDIDVLIDG FRWRKYGQKV VKGNTNPRSY
360 370 380 390
YKCTFQGCGV KKQVERSAAD ERAVLTTYEG RHNHDIPTAL RRS
Length:393
Mass (Da):44,134
Last modified:January 1, 1998 - v1
Checksum:i9057D65B69E12A0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF418309 mRNA Translation: AAL13040.1
AC002338 Genomic DNA Translation: AAC16930.1
AC004165 Genomic DNA Translation: AAM14918.1
CP002685 Genomic DNA Translation: AEC08362.1
AY062720 mRNA Translation: AAL32798.1
AY114650 mRNA Translation: AAM47969.1
AY136318 mRNA Translation: AAM96984.1
AY045813 mRNA Translation: AAK76487.2
BT002338 mRNA Translation: AAN86171.1
BT008482 mRNA Translation: AAP37841.1
PIRiT00575
RefSeqiNP_180584.1, NM_128578.4
UniGeneiAt.12235

Genome annotation databases

EnsemblPlantsiAT2G30250.1; AT2G30250.1; AT2G30250
GeneIDi817575
GrameneiAT2G30250.1; AT2G30250.1; AT2G30250
KEGGiath:AT2G30250

Similar proteinsi

Entry informationi

Entry nameiWRK25_ARATH
AccessioniPrimary (citable) accession number: O22921
Secondary accession number(s): Q94AT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health