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O22921 (WRK25_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable WRKY transcription factor 25
Alternative name(s):
WRKY DNA-binding protein 25
Gene names
Name:WRKY25
Ordered Locus Names:At2g30250
ORF Names:T9D9.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element By similarity.

Subunit structure

Interacts with MKS1. Ref.5

Subcellular location

Nucleus Probable.

Post-translational modification

Phosphorylated by MPK4 Probable. Ref.5

Sequence similarities

Belongs to the WRKY group I family.

Contains 2 WRKY DNA-binding domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MKS1Q8LGD53EBI-1392386,EBI-1392198
MPK4Q390242EBI-1392386,EBI-994375

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Probable WRKY transcription factor 25
PRO_0000133667

Regions

DNA binding160 – 22465WRKY 1
DNA binding322 – 38766WRKY 2

Sequences

Sequence LengthMass (Da)Tools
O22921 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 9057D65B69E12A0C

FASTA39344,134
        10         20         30         40         50         60 
MSSTSFTDLL GSSGVDCYED DEDLRVSGSS FGGYYPERTG SGLPKFKTAQ PPPLPISQSS 

        70         80         90        100        110        120 
HNFTFSDYLD SPLLLSSSHS LISPTTGTFP LQGFNGTTNN HSDFPWQLQS QPSNASSALQ 

       130        140        150        160        170        180 
ETYGVQDHEK KQEMIPNEIA TQNNNQSFGT ERQIKIPAYM VSRNSNDGYG WRKYGQKQVK 

       190        200        210        220        230        240 
KSENPRSYFK CTYPDCVSKK IVETASDGQI TEIIYKGGHN HPKPEFTKRP SQSSLPSSVN 

       250        260        270        280        290        300 
GRRLFNPASV VSEPHDQSEN SSISFDYSDL EQKSFKSEYG EIDEEEEQPE MKRMKREGED 

       310        320        330        340        350        360 
EGMSIEVSKG VKEPRVVVQT ISDIDVLIDG FRWRKYGQKV VKGNTNPRSY YKCTFQGCGV 

       370        380        390 
KKQVERSAAD ERAVLTTYEG RHNHDIPTAL RRS

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana transcription factor WRKY25."
Ulker B., Kushnir S., Somssich I.E.
Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Flower.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The MAP kinase substrate MKS1 is a regulator of plant defense responses."
Andreasson E., Jenkins T., Brodersen P., Thorgrimsen S., Petersen N.H.T., Zhu S., Qiu J.-L., Micheelsen P., Rocher A., Petersen M., Newman M.-A., Bjoern Nielsen H., Hirt H., Somssich I.E., Mattsson O., Mundy J.
EMBO J. 24:2579-2589(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKS1, PHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF418309 mRNA. Translation: AAL13040.1.
AC002338 Genomic DNA. Translation: AAC16930.1.
AC004165 Genomic DNA. Translation: AAM14918.1.
CP002685 Genomic DNA. Translation: AEC08362.1.
AY062720 mRNA. Translation: AAL32798.1.
AY114650 mRNA. Translation: AAM47969.1.
AY136318 mRNA. Translation: AAM96984.1.
AY045813 mRNA. Translation: AAK76487.2.
BT002338 mRNA. Translation: AAN86171.1.
BT008482 mRNA. Translation: AAP37841.1.
IPIIPI00527737.
PIRT00575.
RefSeqNP_180584.1. NM_128578.3.
UniGeneAt.12235.

3D structure databases

ProteinModelPortalO22921.
SMRO22921. Positions 166-224, 326-387.
ModBaseSearch...

Protein-protein interaction databases

IntActO22921. 3 interactions.
STRING3702.AT2G30250.1-P.

Proteomic databases

PRIDEO22921.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30250.1; AT2G30250.1; AT2G30250.
GeneID817575.
KEGGath:AT2G30250.

Organism-specific databases

TAIRAt2g30250.

Phylogenomic databases

eggNOGNOG302838.
HOGENOMHOG000242409.
InParanoidO22921.
KOK13423.
OMADEGMSIE.
PhylomeDBO22921.
ProtClustDBCLSN2913281.

Gene expression databases

GenevestigatorO22921.
GermOnlineAT2G30250. Arabidopsis thaliana.

Family and domain databases

Gene3D2.20.25.80. 2 hits.
InterProIPR003657. WRKY_DNA-bd.
[Graphical view]
PfamPF03106. WRKY. 2 hits.
[Graphical view]
SMARTSM00774. WRKY. 2 hits.
[Graphical view]
SUPFAMSSF118290. SSF118290. 2 hits.
PROSITEPS50811. WRKY. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameWRK25_ARATH
AccessionPrimary (citable) accession number: O22921
Secondary accession number(s): Q94AT4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

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