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Protein

Long chain acyl-CoA synthetase 1

Gene

LACS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).3 Publications

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi225 – 236ATPSequence analysisAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: UniProtKB
  • very long-chain fatty acid-CoA ligase activity Source: TAIR

GO - Biological processi

  • cutin biosynthetic process Source: TAIR
  • fatty acid metabolic process Source: UniProtKB
  • wax biosynthetic process Source: TAIR

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G47240-MONOMER
MetaCyc:AT2G47240-MONOMER
UniPathwayiUPA00199

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 1 (EC:6.2.1.3)
Alternative name(s):
Protein ECERIFERUM 8
Gene namesi
Name:LACS1
Synonyms:CER8
Ordered Locus Names:At2g47240
ORF Names:T8I13.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT2G47240
TAIRilocus:2065195 AT2G47240

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

In stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004014091 – 660Long chain acyl-CoA synthetase 1Add BLAST660

Proteomic databases

PaxDbiO22898
PRIDEiO22898

PTM databases

iPTMnetiO22898

Expressioni

Tissue specificityi

Epidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips.2 Publications

Gene expression databases

ExpressionAtlasiO22898 baseline and differential
GenevisibleiO22898 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47240.1

Structurei

3D structure databases

ProteinModelPortaliO22898
SMRiO22898
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni492 – 516Fatty acid-bindingSequence analysisAdd BLAST25

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
HOGENOMiHOG000159459
InParanoidiO22898
KOiK01897
OMAiTHTAYEC
OrthoDBiEOG093604GF
PhylomeDBiO22898

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

O22898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSFAAKVEE GVKGIDGKPS VGPVYRNLLS EKGFPPIDSE ITTAWDIFSK
60 70 80 90 100
SVEKFPDNNM LGWRRIVDEK VGPYMWKTYK EVYEEVLQIG SALRAAGAEP
110 120 130 140 150
GSRVGIYGVN CPQWIIAMEA CAAHTLICVP LYDTLGSGAV DYIVEHAEID
160 170 180 190 200
FVFVQDTKIK GLLEPDCKCA KRLKAIVSFT NVSDELSHKA SEIGVKTYSW
210 220 230 240 250
IDFLHMGREK PEDTNPPKAF NICTIMYTSG TSGDPKGVVL THQAVATFVV
260 270 280 290 300
GMDLYMDQFE DKMTHDDVYL SFLPLAHILD RMNEEYFFRK GASVGYYHGN
310 320 330 340 350
LNVLRDDIQE LKPTYLAGVP RVFERIHEGI QKALQELNPR RRFIFNALYK
360 370 380 390 400
HKLAWLNRGY SHSKASPMAD FIAFRKIRDK LGGRIRLLVS GGAPLSPEIE
410 420 430 440 450
EFLRVTCCCF VVQGYGLTET LGGTALGFPD EMCMLGTVGI PAVYNEIRLE
460 470 480 490 500
EVSEMGYDPL GENPAGEICI RGQCMFSGYY KNPELTEEVM KDGWFHTGDI
510 520 530 540 550
GEILPNGVLK IIDRKKNLIK LSQGEYVALE HLENIFGQNS VVQDIWVYGD
560 570 580 590 600
SFKSMLVAVV VPNPETVNRW AKDLGFTKPF EELCSFPELK EHIISELKST
610 620 630 640 650
AEKNKLRKFE YIKAVTVETK PFDVERDLVT ATLKNRRNNL LKYYQVQIDE
660
MYRKLASKKI
Length:660
Mass (Da):74,598
Last modified:January 1, 1998 - v1
Checksum:i7CB31F95107E20D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti434M → L in BAD94568 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503751 mRNA Translation: AAM28868.1
AC002337 Genomic DNA Translation: AAB63824.1
CP002685 Genomic DNA Translation: AEC10818.1
CP002685 Genomic DNA Translation: AEC10819.1
CP002685 Genomic DNA Translation: ANM63045.1
AY056380 mRNA Translation: AAL08236.1
AY133648 mRNA Translation: AAM91478.1
AK317315 mRNA Translation: BAH19991.1
AK220997 mRNA Translation: BAD94634.1
AK221992 mRNA Translation: BAD94568.1
PIRiG84912
RefSeqiNP_001031554.1, NM_001036477.1
NP_001318440.1, NM_001337254.1
NP_182246.1, NM_130292.4
UniGeneiAt.21432

Genome annotation databases

EnsemblPlantsiAT2G47240.1; AT2G47240.1; AT2G47240
AT2G47240.2; AT2G47240.2; AT2G47240
AT2G47240.3; AT2G47240.3; AT2G47240
GeneIDi819337
GrameneiAT2G47240.1; AT2G47240.1; AT2G47240
AT2G47240.2; AT2G47240.2; AT2G47240
AT2G47240.3; AT2G47240.3; AT2G47240
KEGGiath:AT2G47240

Entry informationi

Entry nameiLACS1_ARATH
AccessioniPrimary (citable) accession number: O22898
Secondary accession number(s): Q56WP3, Q56ZG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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