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O22898

- LACS1_ARATH

UniProt

O22898 - LACS1_ARATH

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Protein

Long chain acyl-CoA synthetase 1

Gene

LACS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).3 Publications

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi225 – 23612ATPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
  3. very long-chain fatty acid-CoA ligase activity Source: TAIR

GO - Biological processi

  1. cutin biosynthetic process Source: TAIR
  2. fatty acid metabolic process Source: UniProtKB
  3. long-chain fatty acid metabolic process Source: GOC
  4. wax biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G47240-MONOMER.
ARA:GQT-1914-MONOMER.
MetaCyc:AT2G47240-MONOMER.
ReactomeiREACT_235205. Synthesis of very long-chain fatty acyl-CoAs.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Long chain acyl-CoA synthetase 1 (EC:6.2.1.3)
Alternative name(s):
Protein ECERIFERUM 8
Gene namesi
Name:LACS1
Synonyms:CER8
Ordered Locus Names:At2g47240
ORF Names:T8I13.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G47240.

Subcellular locationi

Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

In stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Long chain acyl-CoA synthetase 1PRO_0000401409Add
BLAST

Proteomic databases

PaxDbiO22898.
PRIDEiO22898.

Expressioni

Tissue specificityi

Epidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips.2 Publications

Gene expression databases

GenevestigatoriO22898.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47240.2-P.

Structurei

3D structure databases

ProteinModelPortaliO22898.
SMRiO22898. Positions 46-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni492 – 51625Fatty acid-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1022.
HOGENOMiHOG000159459.
InParanoidiO22898.
KOiK01897.
OMAiCAYFIQG.
PhylomeDBiO22898.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22898-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSFAAKVEE GVKGIDGKPS VGPVYRNLLS EKGFPPIDSE ITTAWDIFSK
60 70 80 90 100
SVEKFPDNNM LGWRRIVDEK VGPYMWKTYK EVYEEVLQIG SALRAAGAEP
110 120 130 140 150
GSRVGIYGVN CPQWIIAMEA CAAHTLICVP LYDTLGSGAV DYIVEHAEID
160 170 180 190 200
FVFVQDTKIK GLLEPDCKCA KRLKAIVSFT NVSDELSHKA SEIGVKTYSW
210 220 230 240 250
IDFLHMGREK PEDTNPPKAF NICTIMYTSG TSGDPKGVVL THQAVATFVV
260 270 280 290 300
GMDLYMDQFE DKMTHDDVYL SFLPLAHILD RMNEEYFFRK GASVGYYHGN
310 320 330 340 350
LNVLRDDIQE LKPTYLAGVP RVFERIHEGI QKALQELNPR RRFIFNALYK
360 370 380 390 400
HKLAWLNRGY SHSKASPMAD FIAFRKIRDK LGGRIRLLVS GGAPLSPEIE
410 420 430 440 450
EFLRVTCCCF VVQGYGLTET LGGTALGFPD EMCMLGTVGI PAVYNEIRLE
460 470 480 490 500
EVSEMGYDPL GENPAGEICI RGQCMFSGYY KNPELTEEVM KDGWFHTGDI
510 520 530 540 550
GEILPNGVLK IIDRKKNLIK LSQGEYVALE HLENIFGQNS VVQDIWVYGD
560 570 580 590 600
SFKSMLVAVV VPNPETVNRW AKDLGFTKPF EELCSFPELK EHIISELKST
610 620 630 640 650
AEKNKLRKFE YIKAVTVETK PFDVERDLVT ATLKNRRNNL LKYYQVQIDE
660
MYRKLASKKI
Length:660
Mass (Da):74,598
Last modified:January 1, 1998 - v1
Checksum:i7CB31F95107E20D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti434 – 4341M → L in BAD94568. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503751 mRNA. Translation: AAM28868.1.
AC002337 Genomic DNA. Translation: AAB63824.1.
CP002685 Genomic DNA. Translation: AEC10818.1.
CP002685 Genomic DNA. Translation: AEC10819.1.
AY056380 mRNA. Translation: AAL08236.1.
AY133648 mRNA. Translation: AAM91478.1.
AK317315 mRNA. Translation: BAH19991.1.
AK220997 mRNA. Translation: BAD94634.1.
AK221992 mRNA. Translation: BAD94568.1.
PIRiG84912.
RefSeqiNP_001031554.1. NM_001036477.1.
NP_182246.1. NM_130292.3.
UniGeneiAt.21432.

Genome annotation databases

EnsemblPlantsiAT2G47240.1; AT2G47240.1; AT2G47240.
AT2G47240.2; AT2G47240.2; AT2G47240.
GeneIDi819337.
KEGGiath:AT2G47240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF503751 mRNA. Translation: AAM28868.1 .
AC002337 Genomic DNA. Translation: AAB63824.1 .
CP002685 Genomic DNA. Translation: AEC10818.1 .
CP002685 Genomic DNA. Translation: AEC10819.1 .
AY056380 mRNA. Translation: AAL08236.1 .
AY133648 mRNA. Translation: AAM91478.1 .
AK317315 mRNA. Translation: BAH19991.1 .
AK220997 mRNA. Translation: BAD94634.1 .
AK221992 mRNA. Translation: BAD94568.1 .
PIRi G84912.
RefSeqi NP_001031554.1. NM_001036477.1.
NP_182246.1. NM_130292.3.
UniGenei At.21432.

3D structure databases

ProteinModelPortali O22898.
SMRi O22898. Positions 46-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G47240.2-P.

Proteomic databases

PaxDbi O22898.
PRIDEi O22898.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G47240.1 ; AT2G47240.1 ; AT2G47240 .
AT2G47240.2 ; AT2G47240.2 ; AT2G47240 .
GeneIDi 819337.
KEGGi ath:AT2G47240.

Organism-specific databases

TAIRi AT2G47240.

Phylogenomic databases

eggNOGi COG1022.
HOGENOMi HOG000159459.
InParanoidi O22898.
KOi K01897.
OMAi CAYFIQG.
PhylomeDBi O22898.

Enzyme and pathway databases

UniPathwayi UPA00199 .
BioCyci ARA:AT2G47240-MONOMER.
ARA:GQT-1914-MONOMER.
MetaCyc:AT2G47240-MONOMER.
Reactomei REACT_235205. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

PROi O22898.

Gene expression databases

Genevestigatori O22898.

Family and domain databases

InterProi IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
    Shockey J.M., Fulda M.S., Browse J.A.
    Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Flower and Silique.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 433-660.
    Strain: cv. Columbia.
  7. "Leaf epicuticular waxes of the eceriferum mutants in Arabidopsis."
    Jenks M.A., Tuttle H.A., Eigenbrode S.D., Feldmann K.A.
    Plant Physiol. 108:369-377(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
    Shockey J.M., Fulda M.S., Browse J.
    Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION.
  9. "Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis."
    Lue S., Song T., Kosma D.K., Parsons E.P., Rowland O., Jenks M.A.
    Plant J. 59:553-564(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  10. "Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis."
    Weng H., Molina I., Shockey J., Browse J.
    Planta 231:1089-1100(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiLACS1_ARATH
AccessioniPrimary (citable) accession number: O22898
Secondary accession number(s): Q56WP3, Q56ZG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3