Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O22898

- LACS1_ARATH

UniProt

O22898 - LACS1_ARATH

Protein

Long chain acyl-CoA synthetase 1

Gene

LACS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).3 Publications

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi225 – 23612ATPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB
    3. very long-chain fatty acid-CoA ligase activity Source: TAIR

    GO - Biological processi

    1. cutin biosynthetic process Source: TAIR
    2. fatty acid metabolic process Source: UniProtKB
    3. long-chain fatty acid metabolic process Source: GOC
    4. wax biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G47240-MONOMER.
    ARA:GQT-1914-MONOMER.
    MetaCyc:AT2G47240-MONOMER.
    UniPathwayiUPA00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long chain acyl-CoA synthetase 1 (EC:6.2.1.3)
    Alternative name(s):
    Protein ECERIFERUM 8
    Gene namesi
    Name:LACS1
    Synonyms:CER8
    Ordered Locus Names:At2g47240
    ORF Names:T8I13.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G47240.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Disruption phenotypei

    In stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C(24)).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 660660Long chain acyl-CoA synthetase 1PRO_0000401409Add
    BLAST

    Proteomic databases

    PaxDbiO22898.
    PRIDEiO22898.

    Expressioni

    Tissue specificityi

    Epidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips.2 Publications

    Gene expression databases

    GenevestigatoriO22898.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G47240.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22898.
    SMRiO22898. Positions 46-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni492 – 51625Fatty acid-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1022.
    HOGENOMiHOG000159459.
    InParanoidiO22898.
    KOiK01897.
    OMAiCAYFIQG.
    PhylomeDBiO22898.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O22898-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSFAAKVEE GVKGIDGKPS VGPVYRNLLS EKGFPPIDSE ITTAWDIFSK    50
    SVEKFPDNNM LGWRRIVDEK VGPYMWKTYK EVYEEVLQIG SALRAAGAEP 100
    GSRVGIYGVN CPQWIIAMEA CAAHTLICVP LYDTLGSGAV DYIVEHAEID 150
    FVFVQDTKIK GLLEPDCKCA KRLKAIVSFT NVSDELSHKA SEIGVKTYSW 200
    IDFLHMGREK PEDTNPPKAF NICTIMYTSG TSGDPKGVVL THQAVATFVV 250
    GMDLYMDQFE DKMTHDDVYL SFLPLAHILD RMNEEYFFRK GASVGYYHGN 300
    LNVLRDDIQE LKPTYLAGVP RVFERIHEGI QKALQELNPR RRFIFNALYK 350
    HKLAWLNRGY SHSKASPMAD FIAFRKIRDK LGGRIRLLVS GGAPLSPEIE 400
    EFLRVTCCCF VVQGYGLTET LGGTALGFPD EMCMLGTVGI PAVYNEIRLE 450
    EVSEMGYDPL GENPAGEICI RGQCMFSGYY KNPELTEEVM KDGWFHTGDI 500
    GEILPNGVLK IIDRKKNLIK LSQGEYVALE HLENIFGQNS VVQDIWVYGD 550
    SFKSMLVAVV VPNPETVNRW AKDLGFTKPF EELCSFPELK EHIISELKST 600
    AEKNKLRKFE YIKAVTVETK PFDVERDLVT ATLKNRRNNL LKYYQVQIDE 650
    MYRKLASKKI 660
    Length:660
    Mass (Da):74,598
    Last modified:January 1, 1998 - v1
    Checksum:i7CB31F95107E20D3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti434 – 4341M → L in BAD94568. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503751 mRNA. Translation: AAM28868.1.
    AC002337 Genomic DNA. Translation: AAB63824.1.
    CP002685 Genomic DNA. Translation: AEC10818.1.
    CP002685 Genomic DNA. Translation: AEC10819.1.
    AY056380 mRNA. Translation: AAL08236.1.
    AY133648 mRNA. Translation: AAM91478.1.
    AK317315 mRNA. Translation: BAH19991.1.
    AK220997 mRNA. Translation: BAD94634.1.
    AK221992 mRNA. Translation: BAD94568.1.
    PIRiG84912.
    RefSeqiNP_001031554.1. NM_001036477.1.
    NP_182246.1. NM_130292.3.
    UniGeneiAt.21432.

    Genome annotation databases

    EnsemblPlantsiAT2G47240.1; AT2G47240.1; AT2G47240.
    AT2G47240.2; AT2G47240.2; AT2G47240.
    GeneIDi819337.
    KEGGiath:AT2G47240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF503751 mRNA. Translation: AAM28868.1 .
    AC002337 Genomic DNA. Translation: AAB63824.1 .
    CP002685 Genomic DNA. Translation: AEC10818.1 .
    CP002685 Genomic DNA. Translation: AEC10819.1 .
    AY056380 mRNA. Translation: AAL08236.1 .
    AY133648 mRNA. Translation: AAM91478.1 .
    AK317315 mRNA. Translation: BAH19991.1 .
    AK220997 mRNA. Translation: BAD94634.1 .
    AK221992 mRNA. Translation: BAD94568.1 .
    PIRi G84912.
    RefSeqi NP_001031554.1. NM_001036477.1.
    NP_182246.1. NM_130292.3.
    UniGenei At.21432.

    3D structure databases

    ProteinModelPortali O22898.
    SMRi O22898. Positions 46-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G47240.2-P.

    Proteomic databases

    PaxDbi O22898.
    PRIDEi O22898.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G47240.1 ; AT2G47240.1 ; AT2G47240 .
    AT2G47240.2 ; AT2G47240.2 ; AT2G47240 .
    GeneIDi 819337.
    KEGGi ath:AT2G47240.

    Organism-specific databases

    TAIRi AT2G47240.

    Phylogenomic databases

    eggNOGi COG1022.
    HOGENOMi HOG000159459.
    InParanoidi O22898.
    KOi K01897.
    OMAi CAYFIQG.
    PhylomeDBi O22898.

    Enzyme and pathway databases

    UniPathwayi UPA00199 .
    BioCyci ARA:AT2G47240-MONOMER.
    ARA:GQT-1914-MONOMER.
    MetaCyc:AT2G47240-MONOMER.

    Miscellaneous databases

    PROi O22898.

    Gene expression databases

    Genevestigatori O22898.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
      Shockey J.M., Fulda M.S., Browse J.A.
      Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
      Tissue: Flower and Silique.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 433-660.
      Strain: cv. Columbia.
    7. "Leaf epicuticular waxes of the eceriferum mutants in Arabidopsis."
      Jenks M.A., Tuttle H.A., Eigenbrode S.D., Feldmann K.A.
      Plant Physiol. 108:369-377(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
      Shockey J.M., Fulda M.S., Browse J.
      Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION.
    9. "Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis."
      Lue S., Song T., Kosma D.K., Parsons E.P., Rowland O., Jenks M.A.
      Plant J. 59:553-564(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    10. "Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis."
      Weng H., Molina I., Shockey J., Browse J.
      Planta 231:1089-1100(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiLACS1_ARATH
    AccessioniPrimary (citable) accession number: O22898
    Secondary accession number(s): Q56WP3, Q56ZG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3