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O22898 (LACS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long chain acyl-CoA synthetase 1

EC=6.2.1.3
Alternative name(s):
Protein ECERIFERUM 8
Gene names
Name:LACS1
Synonyms:CER8
Ordered Locus Names:At2g47240
ORF Names:T8I13.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Acts in both the wax and cutin pathways. Preferentially uses palmitate, palmitoleate, linoleate and eicosenoate. Seems to have a specific activity against very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C24). Ref.7 Ref.9 Ref.10

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA. Ref.1

Cofactor

Magnesium By similarity.

Pathway

Lipid metabolism; fatty acid metabolism.

Subcellular location

Endoplasmic reticulum Ref.10.

Tissue specificity

Epidermal-specific expression along the entire stem. In cauline leaves, was expressed over the entire leaf surface, most strongly in trichomes and guard cells, but not in mesophyll cells. In flowers, the expression was detected in the stigma and filaments of the stamens, and in the carpel was expressed specifically in ovaries. In roots, was expressed in primary and lateral roots, but not in the root tips. Ref.9 Ref.10

Disruption phenotype

In stem, fewer and flatter wax crystals and disorganized cuticle proper and thinner cuticular layer. Reduced amount of wax in all chemical classes on the stem and leaf, except in the very long-chain fatty acid (VLCFA) class with acids longer than 24 carbons (C24). Ref.9 Ref.10

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 660660Long chain acyl-CoA synthetase 1
PRO_0000401409

Regions

Nucleotide binding225 – 23612ATP Potential
Region492 – 51625Fatty acid-binding Potential

Experimental info

Sequence conflict4341M → L in BAD94568. Ref.6

Sequences

Sequence LengthMass (Da)Tools
O22898 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7CB31F95107E20D3

FASTA66074,598
        10         20         30         40         50         60 
MKSFAAKVEE GVKGIDGKPS VGPVYRNLLS EKGFPPIDSE ITTAWDIFSK SVEKFPDNNM 

        70         80         90        100        110        120 
LGWRRIVDEK VGPYMWKTYK EVYEEVLQIG SALRAAGAEP GSRVGIYGVN CPQWIIAMEA 

       130        140        150        160        170        180 
CAAHTLICVP LYDTLGSGAV DYIVEHAEID FVFVQDTKIK GLLEPDCKCA KRLKAIVSFT 

       190        200        210        220        230        240 
NVSDELSHKA SEIGVKTYSW IDFLHMGREK PEDTNPPKAF NICTIMYTSG TSGDPKGVVL 

       250        260        270        280        290        300 
THQAVATFVV GMDLYMDQFE DKMTHDDVYL SFLPLAHILD RMNEEYFFRK GASVGYYHGN 

       310        320        330        340        350        360 
LNVLRDDIQE LKPTYLAGVP RVFERIHEGI QKALQELNPR RRFIFNALYK HKLAWLNRGY 

       370        380        390        400        410        420 
SHSKASPMAD FIAFRKIRDK LGGRIRLLVS GGAPLSPEIE EFLRVTCCCF VVQGYGLTET 

       430        440        450        460        470        480 
LGGTALGFPD EMCMLGTVGI PAVYNEIRLE EVSEMGYDPL GENPAGEICI RGQCMFSGYY 

       490        500        510        520        530        540 
KNPELTEEVM KDGWFHTGDI GEILPNGVLK IIDRKKNLIK LSQGEYVALE HLENIFGQNS 

       550        560        570        580        590        600 
VVQDIWVYGD SFKSMLVAVV VPNPETVNRW AKDLGFTKPF EELCSFPELK EHIISELKST 

       610        620        630        640        650        660 
AEKNKLRKFE YIKAVTVETK PFDVERDLVT ATLKNRRNNL LKYYQVQIDE MYRKLASKKI 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that participate in fatty acid and glycerolipid metabolism."
Shockey J.M., Fulda M.S., Browse J.A.
Plant Physiol. 129:1710-1722(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, ENZYME ACTIVITY.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
Tissue: Flower and Silique.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 433-660.
Strain: cv. Columbia.
[7]"Leaf epicuticular waxes of the eceriferum mutants in Arabidopsis."
Jenks M.A., Tuttle H.A., Eigenbrode S.D., Feldmann K.A.
Plant Physiol. 108:369-377(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases."
Shockey J.M., Fulda M.S., Browse J.
Plant Physiol. 132:1065-1076(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION.
[9]"Arabidopsis CER8 encodes LONG-CHAIN ACYL-COA SYNTHETASE 1 (LACS1) that has overlapping functions with LACS2 in plant wax and cutin synthesis."
Lue S., Song T., Kosma D.K., Parsons E.P., Rowland O., Jenks M.A.
Plant J. 59:553-564(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[10]"Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis."
Weng H., Molina I., Shockey J., Browse J.
Planta 231:1089-1100(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF503751 mRNA. Translation: AAM28868.1.
AC002337 Genomic DNA. Translation: AAB63824.1.
CP002685 Genomic DNA. Translation: AEC10818.1.
CP002685 Genomic DNA. Translation: AEC10819.1.
AY056380 mRNA. Translation: AAL08236.1.
AY133648 mRNA. Translation: AAM91478.1.
AK317315 mRNA. Translation: BAH19991.1.
AK220997 mRNA. Translation: BAD94634.1.
AK221992 mRNA. Translation: BAD94568.1.
PIRG84912.
RefSeqNP_001031554.1. NM_001036477.1.
NP_182246.1. NM_130292.3.
UniGeneAt.21432.

3D structure databases

ProteinModelPortalO22898.
SMRO22898. Positions 46-608.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G47240.2-P.

Proteomic databases

PaxDbO22898.
PRIDEO22898.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G47240.1; AT2G47240.1; AT2G47240.
AT2G47240.2; AT2G47240.2; AT2G47240.
GeneID819337.
KEGGath:AT2G47240.

Organism-specific databases

TAIRAT2G47240.

Phylogenomic databases

eggNOGCOG1022.
HOGENOMHOG000159459.
InParanoidO22898.
KOK01897.
OMACAYFIQG.
PhylomeDBO22898.

Enzyme and pathway databases

BioCycARA:AT2G47240-MONOMER.
ARA:GQT-1914-MONOMER.
MetaCyc:AT2G47240-MONOMER.
UniPathwayUPA00199.

Gene expression databases

GenevestigatorO22898.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO22898.

Entry information

Entry nameLACS1_ARATH
AccessionPrimary (citable) accession number: O22898
Secondary accession number(s): Q56WP3, Q56ZG8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names