Reviewed, UniProtKB/Swiss-Prot O22862 (PER26_ARATH)

Names and origin

Protein names

Recommended name:
    Probable peroxidase 26
      Short name=Atperox P26
    EC=1.11.1.7
Alternative name(s):
    ATP50

Gene names

Name:	PER26
Synonyms:	P26
Ordered Locus Names:	At2g43480
ORF Names:	T1O24.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress Probable. The enzyme activity has to be proved.
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.
Caution	Lacks the distal histidine (here Ser-77), present in the active site, which is one of the conserved features of the classical plant (class III) peroxidase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 335	317	Probable peroxidase 26		PRO_0000023692
Sites
	Active site	73	1	By similarity
	Metal binding	78	1	Calcium 1 By similarity
	Metal binding	81	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	83	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium 1 By similarity
	Metal binding	87	1	Calcium 1 By similarity
	Metal binding	198	1	Iron (heme axial ligand) By similarity
	Metal binding	199	1	Calcium 2 By similarity
	Metal binding	255	1	Calcium 2 By similarity
	Metal binding	258	1	Calcium 2 By similarity
	Binding site	168	1	Substrate; via carbonyl oxygen By similarity
Amino acid modifications
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	259	1	N-linked (GlcNAc...) Potential
	Glycosylation	273	1	N-linked (GlcNAc...) Potential
	Disulfide bond	46 ↔ 122		By similarity
	Disulfide bond	79 ↔ 84		By similarity
	Disulfide bond	128 ↔ 331		By similarity
	Disulfide bond	205 ↔ 237		By similarity
Experimental info
	Sequence conflict	140	1	V → I in AAL84934. Ref.2
	Sequence conflict	189	1	L → R in AAL84934. Ref.2
	Sequence conflict	208	1	V → A in AAL84934. Ref.2
	Sequence conflict	286	1	N → D in AAL84934. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O22862-1 [UniParc]. Last modified November 25, 2002. Version 2. Checksum: CAF4B893D9C3CC24	FASTA	335	37,568
	10 20 30 40 50 60 MVMIHIFLTV MVVGGVSLFP ETAEAIVMGP SMQKLTWHYY KVYNTCENAE NFVRHQVEIF 70 80 90 100 110 120 YKNDKSIAPK LLRLLYSDCF VSGCDASVLL EGPNSEKMAP QNRGLGGFVL IDKIKIVLEQ 130 140 150 160 170 180 RCPGVVSCAD ILNLATRDAV HLAGAPSYPV FTGRRDGLTS DKQTVDLPSP SISWDQAMSY 190 200 210 220 230 240 FKSRGLNVLD MATLLGSHSM GRTHCSYVVD RLYNYNKTGK PSPTMNKYFL SEMAKQCPPR 250 260 270 280 290 300 TRKGQTDPLV YLNPDSGSNH SFTSSFYSRI LSNKSVLEVD QQLLYNDDTK QISKEFSEGF 310 320 330 EDFRKSFALS MSKMGAINVL TKTEGEIRKD CRHIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002335 Genomic DNA. Translation: AAB64327.1. Different initiation. AY078928 mRNA. Translation: AAL84934.1.
IPI	IPI00520349.
RefSeq	NP_181876.2.
UniGene	At.36889
3D structure databases
HSSP	HSSP built from PDB template 1PA2 based on UniProtKB Q42578.
ModBase	Search...
Protein family/group databases
PeroxiBase	119. AtPrx26.
Genome annotation databases
GeneID	818949.
GenomeReviews	Gene locus AT2G43480 in contig CT485783_GR.
KEGG	ath:AT2G43480.
NMPDR	fig|3702.1.peg.11504.
Organism-specific databases
GeneFarm	1853. 61.
TAIR	At2g43480.
Phylogenomic databases
OMA	O22862. LLYSDCF.
Enzyme and pathway databases
BRENDA	1.11.1.7. 302.
Gene expression databases
ArrayExpress	O22862.
GermOnline	AT2G43480. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PER26_ARATH

Accession

Primary (citable) accession number: O22862
Secondary accession number(s): Q8RY36

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	November 25, 2002
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)