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Protein

Probable peroxidase 26

Gene

PER26

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress (Probable). The enzyme activity has to be proved.Curated

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731By similarity
Metal bindingi78 – 781Calcium 1PROSITE-ProRule annotation
Metal bindingi81 – 811Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi83 – 831Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi85 – 851Calcium 1PROSITE-ProRule annotation
Metal bindingi87 – 871Calcium 1PROSITE-ProRule annotation
Binding sitei168 – 1681Substrate; via carbonyl oxygenPROSITE-ProRule annotation
Metal bindingi198 – 1981Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi199 – 1991Calcium 2PROSITE-ProRule annotation
Metal bindingi255 – 2551Calcium 2PROSITE-ProRule annotation
Metal bindingi258 – 2581Calcium 2PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G43480-MONOMER.

Protein family/group databases

PeroxiBasei119. AtPrx26.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable peroxidase 26 (EC:1.11.1.7)
Short name:
Atperox P26
Alternative name(s):
ATP50
Gene namesi
Name:PER26
Synonyms:P26
Ordered Locus Names:At2g43480
ORF Names:T1O24.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43480.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 335317Probable peroxidase 26PRO_0000023692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 122PROSITE-ProRule annotation
Disulfide bondi79 ↔ 84PROSITE-ProRule annotation
Disulfide bondi128 ↔ 331PROSITE-ProRule annotation
Disulfide bondi205 ↔ 237PROSITE-ProRule annotation
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO22862.
PRIDEiO22862.

PTM databases

iPTMnetiO22862.

Expressioni

Gene expression databases

GenevisibleiO22862. AT.

Interactioni

Protein-protein interaction databases

BioGridi4285. 1 interaction.
MINTiMINT-8069091.
STRINGi3702.AT2G43480.1.

Structurei

3D structure databases

ProteinModelPortaliO22862.
SMRiO22862. Positions 34-335.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IG0P. Eukaryota.
ENOG410YECP. LUCA.
HOGENOMiHOG000237556.
InParanoidiO22862.
KOiK00430.
OMAiLYSDCFV.
PhylomeDBiO22862.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMIHIFLTV MVVGGVSLFP ETAEAIVMGP SMQKLTWHYY KVYNTCENAE
60 70 80 90 100
NFVRHQVEIF YKNDKSIAPK LLRLLYSDCF VSGCDASVLL EGPNSEKMAP
110 120 130 140 150
QNRGLGGFVL IDKIKIVLEQ RCPGVVSCAD ILNLATRDAV HLAGAPSYPV
160 170 180 190 200
FTGRRDGLTS DKQTVDLPSP SISWDQAMSY FKSRGLNVLD MATLLGSHSM
210 220 230 240 250
GRTHCSYVVD RLYNYNKTGK PSPTMNKYFL SEMAKQCPPR TRKGQTDPLV
260 270 280 290 300
YLNPDSGSNH SFTSSFYSRI LSNKSVLEVD QQLLYNDDTK QISKEFSEGF
310 320 330
EDFRKSFALS MSKMGAINVL TKTEGEIRKD CRHIN
Length:335
Mass (Da):37,568
Last modified:November 25, 2002 - v2
Checksum:iCAF4B893D9C3CC24
GO

Sequence cautioni

The sequence AAB64327.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1401V → I in AAL84934 (PubMed:14593172).Curated
Sequence conflicti189 – 1891L → R in AAL84934 (PubMed:14593172).Curated
Sequence conflicti208 – 2081V → A in AAL84934 (PubMed:14593172).Curated
Sequence conflicti286 – 2861N → D in AAL84934 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64327.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10275.1.
AY078928 mRNA. Translation: AAL84934.1.
RefSeqiNP_181876.2. NM_129909.2.
UniGeneiAt.36889.

Genome annotation databases

EnsemblPlantsiAT2G43480.1; AT2G43480.1; AT2G43480.
GeneIDi818949.
GrameneiAT2G43480.1; AT2G43480.1; AT2G43480.
KEGGiath:AT2G43480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64327.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC10275.1.
AY078928 mRNA. Translation: AAL84934.1.
RefSeqiNP_181876.2. NM_129909.2.
UniGeneiAt.36889.

3D structure databases

ProteinModelPortaliO22862.
SMRiO22862. Positions 34-335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4285. 1 interaction.
MINTiMINT-8069091.
STRINGi3702.AT2G43480.1.

Protein family/group databases

PeroxiBasei119. AtPrx26.

PTM databases

iPTMnetiO22862.

Proteomic databases

PaxDbiO22862.
PRIDEiO22862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43480.1; AT2G43480.1; AT2G43480.
GeneIDi818949.
GrameneiAT2G43480.1; AT2G43480.1; AT2G43480.
KEGGiath:AT2G43480.

Organism-specific databases

TAIRiAT2G43480.

Phylogenomic databases

eggNOGiENOG410IG0P. Eukaryota.
ENOG410YECP. LUCA.
HOGENOMiHOG000237556.
InParanoidiO22862.
KOiK00430.
OMAiLYSDCFV.
PhylomeDBiO22862.

Enzyme and pathway databases

BioCyciARA:AT2G43480-MONOMER.

Miscellaneous databases

PROiO22862.

Gene expression databases

GenevisibleiO22862. AT.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
    Tognolli M., Penel C., Greppin H., Simon P.
    Gene 288:129-138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPER26_ARATH
AccessioniPrimary (citable) accession number: O22862
Secondary accession number(s): Q8RY36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 25, 2002
Last modified: February 17, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Caution

Lacks the distal histidine (here Ser-77), present in the active site, which is one of the conserved features of the classical plant (class III) peroxidase family.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.