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Protein

Probable glutathione peroxidase 3, mitochondrial

Gene

GPX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May constitute a glutathione peroxidase-like protective system against oxidative stresses. Involved positively in abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Oxidizes and represses target proteins (e.g. the phosphatase activity of ABI1 and ABI2) when oxidized by H2O2, probably after ABA signaling. Modulates the calcium channel activity in guard cells in response to ABA or H2O2. Confers tolerance to drought stress, by enhancing the ABA-dependent stomatal closure.1 Publication

Miscellaneous

The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3.

Catalytic activityi

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Enzyme regulationi

The redox states are modulated by H2O2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei80By similarity1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • cellular response to water deprivation Source: TAIR
  • response to hydrogen peroxide Source: TAIR

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processAbscisic acid signaling pathway, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G43350-MONOMER
BRENDAi1.11.1.9 399
ReactomeiR-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives

Protein family/group databases

PeroxiBasei2501 AtGPx03

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione peroxidase 3, mitochondrial (EC:1.11.1.9)
Gene namesi
Name:GPX3
Ordered Locus Names:At2g43350
ORF Names:T1O24.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G43350
TAIRilocus:2058233 AT2G43350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Leaves of plants lacking GPX3 are 1 degree Celsius lower than normal plants, whereas leaves from plants over-expressing GPX3 are 1.2 degrees Celsius higher, probably because of the impaired evapotranspiration.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 12MitochondrionSequence analysisAdd BLAST12
ChainiPRO_000004545813 – 206Probable glutathione peroxidase 3, mitochondrialAdd BLAST194

Proteomic databases

PaxDbiO22850
PRIDEiO22850

PTM databases

SwissPalmiO22850

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

By osmotic stress, H2O2, drought stress, and metals. Regulated by abscisic acid (ABA); down-regulated by 1 mM ABA (PubMed:14617062), whereas induced by 60 µM ABA (PubMed:16998070).2 Publications

Gene expression databases

ExpressionAtlasiO22850 baseline and differential
GenevisibleiO22850 AT

Interactioni

Subunit structurei

Interacts with ABI1 and ABI2.1 Publication

Protein-protein interaction databases

BioGridi4273, 3 interactors
STRINGi3702.AT2G43350.1

Structurei

3D structure databases

ProteinModelPortaliO22850
SMRiO22850
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1651 Eukaryota
COG0386 LUCA
HOGENOMiHOG000277054
InParanoidiO22850
KOiK00432
OMAiEMNILYA
OrthoDBiEOG09360NO2
PhylomeDBiO22850

Family and domain databases

CDDicd00340 GSH_Peroxidase, 1 hit
InterProiView protein in InterPro
IPR000889 Glutathione_peroxidase
IPR029759 GPX_AS
IPR029760 GPX_CS
IPR036249 Thioredoxin-like_sf
PANTHERiPTHR11592 PTHR11592, 1 hit
PfamiView protein in Pfam
PF00255 GSHPx, 1 hit
PIRSFiPIRSF000303 Glutathion_perox, 1 hit
PRINTSiPR01011 GLUTPROXDASE
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00460 GLUTATHIONE_PEROXID_1, 1 hit
PS00763 GLUTATHIONE_PEROXID_2, 1 hit
PS51355 GLUTATHIONE_PEROXID_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSSRWVNQ RATSKIKKFI LFLGVAFVFY LYRYPSSPST VEQSSTSIYN
60 70 80 90 100
ISVKDIEGKD VSLSKFTGKV LLIVNVASKC GLTHGNYKEM NILYAKYKTQ
110 120 130 140 150
GFEILAFPCN QFGSQEPGSN MEIKETVCNI FKAEFPIFDK IEVNGKNTCP
160 170 180 190 200
LYNFLKEQKG GLFGDAIKWN FAKFLVDRQG NVVDRYAPTT SPLEIEKDIV

KLLASA
Length:206
Mass (Da):23,258
Last modified:January 1, 1998 - v1
Checksum:iC060AE62F079BD7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA Translation: AAB64335.1
CP002685 Genomic DNA Translation: AEC10254.1
CP002685 Genomic DNA Translation: AEC10255.1
AY065372 mRNA Translation: AAL38813.1
AY096479 mRNA Translation: AAM20119.1
AY087030 mRNA Translation: AAM64591.1
PIRiA84865
RefSeqiNP_001189742.1, NM_001202813.1
NP_181863.1, NM_129896.4
UniGeneiAt.28611
At.66417

Genome annotation databases

EnsemblPlantsiAT2G43350.1; AT2G43350.1; AT2G43350
AT2G43350.2; AT2G43350.2; AT2G43350
GeneIDi818936
GrameneiAT2G43350.1; AT2G43350.1; AT2G43350
AT2G43350.2; AT2G43350.2; AT2G43350
KEGGiath:AT2G43350

Similar proteinsi

Entry informationi

Entry nameiGPX3_ARATH
AccessioniPrimary (citable) accession number: O22850
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health