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Protein

Probable glutathione peroxidase 3, mitochondrial

Gene

GPX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May constitute a glutathione peroxidase-like protective system against oxidative stresses. Involved positively in abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Oxidizes and represses target proteins (e.g. the phosphatase activity of ABI1 and ABI2) when oxidized by H2O2, probably after ABA signaling. Modulates the calcium channel activity in guard cells in response to ABA or H2O2. Confers tolerance to drought stress, by enhancing the ABA-dependent stomatal closure.1 Publication

Catalytic activityi

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Enzyme regulationi

The redox states are modulated by H2O2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei80 – 801By similarity

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • cellular response to water deprivation Source: TAIR
  • response to hydrogen peroxide Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Abscisic acid signaling pathway, Stress response

Enzyme and pathway databases

BioCyciARA:AT2G43350-MONOMER.
ARA:GQT-2179-MONOMER.
BRENDAi1.11.1.9. 399.
ReactomeiR-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.

Protein family/group databases

PeroxiBasei2501. AtGPx03.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutathione peroxidase 3, mitochondrial (EC:1.11.1.9)
Gene namesi
Name:GPX3
Ordered Locus Names:At2g43350
ORF Names:T1O24.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G43350.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • mitochondrion Source: TAIR
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Leaves of plants lacking GPX3 are 1 degree Celsius lower than normal plants, whereas leaves from plants over-expressing GPX3 are 1.2 degrees Celsius higher, probably because of the impaired evapotranspiration.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1212MitochondrionSequence analysisAdd
BLAST
Chaini13 – 206194Probable glutathione peroxidase 3, mitochondrialPRO_0000045458Add
BLAST

Proteomic databases

PaxDbiO22850.
PRIDEiO22850.

PTM databases

SwissPalmiO22850.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Inductioni

By osmotic stress, H2O2, drought stress, and metals. Regulated by abscisic acid (ABA); down-regulated by 1 mM ABA (PubMed:14617062), whereas induced by 60 µM ABA (PubMed:16998070).2 Publications

Gene expression databases

GenevisibleiO22850. AT.

Interactioni

Subunit structurei

Interacts with ABI1 and ABI2.1 Publication

Protein-protein interaction databases

BioGridi4273. 3 interactions.
STRINGi3702.AT2G43350.1.

Structurei

3D structure databases

ProteinModelPortaliO22850.
SMRiO22850. Positions 47-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOGENOMiHOG000277054.
InParanoidiO22850.
KOiK00432.
OMAiYKEMNIL.
PhylomeDBiO22850.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSSRWVNQ RATSKIKKFI LFLGVAFVFY LYRYPSSPST VEQSSTSIYN
60 70 80 90 100
ISVKDIEGKD VSLSKFTGKV LLIVNVASKC GLTHGNYKEM NILYAKYKTQ
110 120 130 140 150
GFEILAFPCN QFGSQEPGSN MEIKETVCNI FKAEFPIFDK IEVNGKNTCP
160 170 180 190 200
LYNFLKEQKG GLFGDAIKWN FAKFLVDRQG NVVDRYAPTT SPLEIEKDIV

KLLASA
Length:206
Mass (Da):23,258
Last modified:January 1, 1998 - v1
Checksum:iC060AE62F079BD7F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64335.1.
CP002685 Genomic DNA. Translation: AEC10254.1.
CP002685 Genomic DNA. Translation: AEC10255.1.
AY065372 mRNA. Translation: AAL38813.1.
AY096479 mRNA. Translation: AAM20119.1.
AY087030 mRNA. Translation: AAM64591.1.
PIRiA84865.
RefSeqiNP_001189742.1. NM_001202813.1.
NP_181863.1. NM_129896.3.
UniGeneiAt.28611.
At.66417.

Genome annotation databases

EnsemblPlantsiAT2G43350.1; AT2G43350.1; AT2G43350.
AT2G43350.2; AT2G43350.2; AT2G43350.
GeneIDi818936.
GrameneiAT2G43350.1; AT2G43350.1; AT2G43350.
AT2G43350.2; AT2G43350.2; AT2G43350.
KEGGiath:AT2G43350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002335 Genomic DNA. Translation: AAB64335.1.
CP002685 Genomic DNA. Translation: AEC10254.1.
CP002685 Genomic DNA. Translation: AEC10255.1.
AY065372 mRNA. Translation: AAL38813.1.
AY096479 mRNA. Translation: AAM20119.1.
AY087030 mRNA. Translation: AAM64591.1.
PIRiA84865.
RefSeqiNP_001189742.1. NM_001202813.1.
NP_181863.1. NM_129896.3.
UniGeneiAt.28611.
At.66417.

3D structure databases

ProteinModelPortaliO22850.
SMRiO22850. Positions 47-203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4273. 3 interactions.
STRINGi3702.AT2G43350.1.

Protein family/group databases

PeroxiBasei2501. AtGPx03.

PTM databases

SwissPalmiO22850.

Proteomic databases

PaxDbiO22850.
PRIDEiO22850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G43350.1; AT2G43350.1; AT2G43350.
AT2G43350.2; AT2G43350.2; AT2G43350.
GeneIDi818936.
GrameneiAT2G43350.1; AT2G43350.1; AT2G43350.
AT2G43350.2; AT2G43350.2; AT2G43350.
KEGGiath:AT2G43350.

Organism-specific databases

TAIRiAT2G43350.

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOGENOMiHOG000277054.
InParanoidiO22850.
KOiK00432.
OMAiYKEMNIL.
PhylomeDBiO22850.

Enzyme and pathway databases

BioCyciARA:AT2G43350-MONOMER.
ARA:GQT-2179-MONOMER.
BRENDAi1.11.1.9. 399.
ReactomeiR-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.

Miscellaneous databases

PROiO22850.

Gene expression databases

GenevisibleiO22850. AT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways."
    Rodriguez Milla M.A., Maurer A., Rodriguez Huete A., Gustafson J.P.
    Plant J. 36:602-615(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, INDUCTION.
  6. "An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses."
    Miao Y., Lv D., Wang P., Wang X.-C., Chen J., Miao C., Song C.-P.
    Plant Cell 18:2749-2766(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INDUCTION, INTERACTION WITH ABI1 AND ABI2, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGPX3_ARATH
AccessioniPrimary (citable) accession number: O22850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.