Reviewed, UniProtKB/Swiss-Prot O22850 (GPX3_ARATH)

Names and origin

Protein names

Recommended name:
    Probable glutathione peroxidase 3, mitochondrial
    EC=1.11.1.9

Gene names

Name:	GPX3
Ordered Locus Names:	At2g43350
ORF Names:	T1O24.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May constitute a glutathionine peroxidase-like protective system against oxidative stresses. Involved positively in abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Oxidizes and represses target proteins (e.g. the phosphatase activity of ABI1 and ABI2) when oxidized by H2O2, probably after ABA signaling. Modulates the calcium channel activity in guard cells in response to ABA or H2O2. Confers tolerance to drought stress, by enhancing the ABA-dependent stomatal closure. Ref.5
Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Enzyme regulation	The redox states are modulated by H2O2. Ref.5
Subunit structure	Interacts with ABI1 and ABI2. Ref.5
Subcellular location	Mitochondrion Potential.
Tissue specificity	Ubiquitous. Ref.4
Induction	By osmotic stress, H2O2, drought stress, and metals. Regulated by abscisic acid (ABA); down-regulated by 1mM ABA (Ref.4), whereas induced by 60uM ABA (Ref.5). Ref.5 Ref.4
Disruption phenotype	Leaves of plants lacking GPX3 are 1 degree Celsius lower than normal plants, whereas leaves from plants over-expressing GPX3 are 1.2 degrees Celsius higher, probably because of the impaired evapotranspiration. Ref.5
Miscellaneous	The reduced form of ABI2 is converted to the oxidized form by the addition of oxidized GPX3.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway Stress response
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid mediated signaling Ref.5 Inferred from mutant phenotype. Source: TAIR cellular response to water deprivation Ref.5 Inferred from mutant phenotype. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to hydrogen peroxide Ref.5 Inferred from mutant phenotype. Source: TAIR
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 12	12	Mitochondrion Potential
	Chain	13 – 206	194	Probable glutathione peroxidase 3, mitochondrial		PRO_0000045458
Sites
	Active site	80	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22850-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C060AE62F079BD7F	FASTA	206	23,258
	10 20 30 40 50 60 MPRSSRWVNQ RATSKIKKFI LFLGVAFVFY LYRYPSSPST VEQSSTSIYN ISVKDIEGKD 70 80 90 100 110 120 VSLSKFTGKV LLIVNVASKC GLTHGNYKEM NILYAKYKTQ GFEILAFPCN QFGSQEPGSN 130 140 150 160 170 180 MEIKETVCNI FKAEFPIFDK IEVNGKNTCP LYNFLKEQKG GLFGDAIKWN FAKFLVDRQG 190 200 NVVDRYAPTT SPLEIEKDIV KLLASA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways." Rodriguez Milla M.A., Maurer A., Rodriguez Huete A., Gustafson J.P. Plant J. 36:602-615(2003) [PubMed: 14617062] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, INDUCTION.
[5]	"An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses." Miao Y., Lv D., Wang P., Wang X.-C., Chen J., Miao C., Song C.-P. Plant Cell 18:2749-2766(2006) [PubMed: 16998070] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, INDUCTION, INTERACTION WITH ABI1 AND ABI2, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC002335 Genomic DNA. Translation: AAB64335.1. AY065372 mRNA. Translation: AAL38813.1. AY096479 mRNA. Translation: AAM20119.1. AY087030 mRNA. Translation: AAM64591.1.
IPI	IPI00546051.
PIR	A84865.
RefSeq	NP_181863.1.
UniGene	At.28611
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Protein family/group databases
PeroxiBase	2501. AtGPx03.
Proteomic databases
PRIDE	O22850.
Genome annotation databases
GeneID	818936.
GenomeReviews	Gene locus AT2G43350 in contig CT485783_GR.
KEGG	ath:AT2G43350.
NMPDR	fig|3702.1.peg.11491.
Organism-specific databases
GeneFarm	2051. 163.
TAIR	At2g43350.
Phylogenomic databases
OMA	O22850. AQNGNDL.
Enzyme and pathway databases
BRENDA	1.11.1.9. 302.
Gene expression databases
ArrayExpress	O22850.
GermOnline	AT2G43350. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX3_ARATH

Accession

Primary (citable) accession number: O22850

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)