O22833 (LRK54_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-type lectin-domain containing receptor kinase V.4
Short name=Arabidopsis thaliana lectin-receptor kinase c1
Short name=AthlecRK-c1
Short name=LecRK-V.4
EC=2.7.11.1

Gene names

Name:	LECRK54
Synonyms:	LECRKC1
Ordered Locus Names:	At2g43700
ORF Names:	F18O19.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	In the C-terminal section; belongs to the protein kinase superfamily. Ser/Thr protein kinase family. In the N-terminal section; belongs to the leguminous lectin family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Lectin Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 658	633	L-type lectin-domain containing receptor kinase V.4		PRO_0000403092
Regions
	Topological domain	26 – 280	255	Extracellular Potential
	Transmembrane	281 – 301	21	Helical; Potential
	Topological domain	302 – 658	357	Cytoplasmic Potential
	Domain	334 – 592	259	Protein kinase
	Nucleotide binding	340 – 348	9	ATP By similarity
	Region	26 – 248	223	Legume-lectin like
Sites
	Active site	460	1	Proton acceptor By similarity
	Binding site	363	1	ATP By similarity
Amino acid modifications
	Glycosylation	66	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O22833 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 66E871108F732BB1	FASTA	658	73,906
	10 20 30 40 50 60 MSRTIGSRVI FLILALFCCT ENSRGKLVMQ GSAGFFKGYR TLTSTKKHAY GQAFEDEIVP 70 80 90 100 110 120 FKNSANDTVT SFSVTFFFAI APEDKHKGAH GMAFVISPTR GITGASADQY LGIFNKANNG 130 140 150 160 170 180 DSSNHVIAVE LDINKDEEFG DINDNHVGIN INGMRSIKFA PAGYYDQEGQ FKDLSLISGS 190 200 210 220 230 240 LLRVTILYSQ MEKQLNVTLS SPEEAYYPNK PLLSLNQDLS PYILENMYVG FSASTGSVRA 250 260 270 280 290 300 MHYMLSWFVH GGVDVPNLDL GIPTFPPYPK EKSLVYRIVL VTSLALVLFV ALVASALSIF 310 320 330 340 350 360 FYRRHKKVKE VLEEWEIQCG PHRFAYKELF KATKGFKQLL GKGGFGQVFK GTLPGSDAEI 370 380 390 400 410 420 AVKRISHDSK QGMQEFLAEI STIGRLRHQN LVRLQGYCRY KEELYLVYDF MPNGSLDKYL 430 440 450 460 470 480 YHRANQEQLT WNQRFKIIKD IASALCYLHH EWVQVVIHRD IKPANVLIDH QMNARLGDFG 490 500 510 520 530 540 LAKLYDQGYD PQTSRVAGTF WYIAPELIRS GRATTGTDVY AFGLFMLEVS CGRRLIERRT 550 560 570 580 590 600 ASDEVVLAEW TLKCWENGDI LEAVNDGIRH EDNREQLELV LKLGVLCSHQ AVAIRPDMSK 610 620 630 640 650 VVQILGGDLQ LPDNLLDIVK AEKVRMWSET SESVLGVLTS QGSIGTLTLT EPFTSRGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins." Herve C., Serres J., Dabos P., Canut H., Barre A., Rouge P., Lescure B. Plant Mol. Biol. 39:671-682(1999) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[5]	"Lectin receptor kinases in plants." Barre A., Herve C., Lescure B., Rouge P. Crit. Rev. Plant Sci. 21:379-399(2002) Cited for: GENE FAMILY.
[6]	"Arabidopsis L-type lectin receptor kinases: phylogeny, classification, and expression profiles." Bouwmeester K., Govers F. J. Exp. Bot. 60:4383-4396(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002333 Genomic DNA. Translation: AAB64036.1. CP002685 Genomic DNA. Translation: AEC10309.1. AY074631 mRNA. Translation: AAL69447.1.
IPI	IPI00544864.
PIR	D84869.
RefSeq	NP_181898.1. NM_129932.6.
UniGene	At.37031.
3D structure databases
HSSP	HSSP built from PDB template 1G9F based on UniProtKB P05046.
ProteinModelPortal	O22833.
SMR	O22833. Positions 27-250, 267-608.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G43700.1-P.
Proteomic databases
PaxDb	O22833.
PRIDE	O22833.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G43700.1; AT2G43700.1; AT2G43700.
GeneID	818972.
KEGG	ath:AT2G43700.
Organism-specific databases
GeneFarm	92. 4.
TAIR	At2g43700.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116555.
InParanoid	O22833.
OMA	KNSANDT.
PhylomeDB	O22833.
Gene expression databases
Genevestigator	O22833.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR011009. Kinase-like_dom. IPR001220. Legume_lectin_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00139. Lectin_legB. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00307. LECTIN_LEGUME_BETA. False negative. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LRK54_ARATH

Accession

Primary (citable) accession number: O22833

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program