O22818 (PGLR6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable polygalacturonase At2g43860
Short name=PG
EC=3.2.1.15

Alternative name(s):
Pectinase At2g43860

Gene names

Ordered Locus Names:	At2g43860
ORF Names:	F18O19.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in developing and germinated seeds in the chalazal region immediately adjacent to the site of penetration of the radicle through the testa. Ref.4 Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 3 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 405	379	Probable polygalacturonase At2g43860		PRO_0000367918
Regions
	Repeat	184 – 210	27	PbH1 1
	Repeat	211 – 232	22	PbH1 2
	Repeat	264 – 285	22	PbH1 3
Sites
	Active site	225	1	Proton donor By similarity
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22818 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 556D65570A44D195	FASTA	405	43,462
	10 20 30 40 50 60 MVYNTSCLIL PLALIILDFT LISSLAHPIP STLNVLSYGA KPDGSKDSTK AFLAAWDVAC 70 80 90 100 110 120 ASANPTTIIV PKGRFLVGNL VFHGNECKQA PISIRIAGSI VAPEDFRIIA SSKHWIWFED 130 140 150 160 170 180 VTDVSIYGGI LDAQGTSLWK CKNNGGHNCP TGAKSLVFSG SNNIKISGLT SINSQKFHIV 190 200 210 220 230 240 IDNSNNVNID GVKVSADENS PNTDGIHVES SHSVHITNSR IGTGDDCISI GPGSTNVFIQ 250 260 270 280 290 300 TIRCGPGHGI SIGSLGRAEE EQGVDNVTVS NVDFMGTNNG VRIKTWGKDS NSFARNIVFQ 310 320 330 340 350 360 HINMKMVKNP IIIDQHYCLH KPCPKQESGV KVSNVRYEDI HGTSNTEVAV LLDCSKEKPC 370 380 390 400 TGIVMDDVNL VSVHRPAQAS CDNANGSAND VVPFTPCLKR EIIIT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Patterns of expansion and expression divergence in the plant polygalacturonase gene family." Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E. Genome Biol. 7:R87.1-R87.14(2006) [PubMed: 17010199] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana." Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A. J. Exp. Bot. 58:3719-3730(2007) [PubMed: 17928369] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002333 Genomic DNA. Translation: AAB64020.1. CP002685 Genomic DNA. Translation: AEC10336.1. DQ056577 mRNA. Translation: AAY78726.1.
IPI	IPI00540510.
PIR	D84871.
RefSeq	NP_181914.1. NM_129948.1.
UniGene	At.53113.
3D structure databases
ProteinModelPortal	O22818.
SMR	O22818. Positions 43-375.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PRIDE	O22818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G43860.1; AT2G43860.1; AT2G43860.
GeneID	818990.
GenomeReviews	Gene locus AT2G43860 in contig CT485783_GR.
KEGG	ath:AT2G43860.
NMPDR	fig|3702.1.peg.11549.
Organism-specific databases
GeneFarm	16. 2.
TAIR	At2g43860.
Phylogenomic databases
eggNOG	COG5434.
GeneTree	EPGT00050000001253.
HOGENOM	HBG744658.
InParanoid	O22818.
OMA	CASANPT.
PhylomeDB	O22818.
ProtClustDB	CLSN2912911.
Gene expression databases
ArrayExpress	O22818.
Genevestigator	O22818.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 3 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR6_ARATH

Accession

Primary (citable) accession number: O22818

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 1, 1998
Last modified:	September 21, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program