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Protein

Pre-mRNA-processing factor 19 homolog 2

Gene

PRP19B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair (By similarity). Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity.By similarity2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Immunity, Innate immunity, mRNA processing, mRNA splicing, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6781823 Formation of TC-NER Pre-Incision Complex
R-ATH-6782135 Dual incision in TC-NER
R-ATH-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-ATH-72163 mRNA Splicing - Major Pathway
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 19 homolog 2Curated (EC:2.3.2.271 Publication)
Alternative name(s):
MOS4-associated complex protein 3B
Short name:
MAC protein 3B
Plant U-box protein 60
RING-type E3 ubiquitin transferase PRP19 2Curated
U-box domain-containing protein 60
Gene namesi
Name:PRP19B
Synonyms:MAC3B, PUB60
Ordered Locus Names:At2g33340
ORF Names:F4P9.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G33340
TAIRilocus:2051094 AT2G33340

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003221381 – 525Pre-mRNA-processing factor 19 homolog 2Add BLAST525

Proteomic databases

PaxDbiO22785
PRIDEiO22785

PTM databases

iPTMnetiO22785

Expressioni

Gene expression databases

ExpressionAtlasiO22785 baseline and differential
GenevisibleiO22785 AT

Interactioni

Subunit structurei

Homotetramer. Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 which is related to the PRP19C/Prp19 complex/NTC/Nineteen complex identified in other organisms. Associated with the spliceosome.By similarity1 Publication

Protein-protein interaction databases

BioGridi3244, 3 interactors
IntActiO22785, 2 interactors
STRINGi3702.AT2G33340.1

Structurei

3D structure databases

ProteinModelPortaliO22785
SMRiO22785
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 70U-boxAdd BLAST70
Repeati220 – 261WD 1Add BLAST42
Repeati262 – 301WD 2Add BLAST40
Repeati307 – 346WD 3Add BLAST40
Repeati351 – 390WD 4Add BLAST40
Repeati393 – 431WD 5Add BLAST39
Repeati433 – 469WD 6Add BLAST37
Repeati478 – 517WD 7Add BLAST40

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi409 – 424DWD boxAdd BLAST16

Domaini

The DWD box is required for interaction with DDB1A.By similarity

Sequence similaritiesi

Belongs to the WD repeat PRP19 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0289 Eukaryota
ENOG410XPQV LUCA
HOGENOMiHOG000177308
InParanoidiO22785
KOiK10599
OMAiTLHTASI
OrthoDBiEOG09360734
PhylomeDBiO22785

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013915 Pre-mRNA_splic_Prp19
IPR038959 Prp19
IPR003613 Ubox_domain
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR43995 PTHR43995, 1 hit
PfamiView protein in Pfam
PF08606 Prp19, 1 hit
PF00400 WD40, 4 hits
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O22785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNCAISGEVP VEPVVSTKSG LLFERRLIER HISDYGKCPV TGEPLTIDDI
60 70 80 90 100
VPIKTGEIIK PKTLHTASIP GLLGTFQNEW DGLMLSNFAL EQQLHTARQE
110 120 130 140 150
LSHALYQHDS ACRVIARLKK ERDEARQLLA EVERHIPAAP EAVTANAALS
160 170 180 190 200
NGKRAAVDEE LGPDAKKLCP GISAEIITEL TDCNAALSQK RKKRQIPQTL
210 220 230 240 250
ASIDTLERFT QLSSHPLHKT NKPGICSMDI LHSKDVIATG GVDATAVLFD
260 270 280 290 300
RPSGQILSTL TGHSKKVTSV KFVGDSDLVL TASADKTVRI WRNPGDGNYA
310 320 330 340 350
CGYTLNDHSA EVRAVTVHPT NKYFVSASLD GTWCFYDLSS GSCLAQVSDD
360 370 380 390 400
SKNVDYTAAA FHPDGLILGT GTSQSVVKIW DVKSQANVAK FDGHTGEVTA
410 420 430 440 450
ISFSENGYFL ATAAEDGVRL WDLRKLRNFK SFLSADANSV EFDPSGSYLG
460 470 480 490 500
IAASDIKVYQ TASVKAEWNL IKTLPDLSGT GKATCVKFGS DAQYVAVGSM
510 520
DRNLRIFGLP GDEKANVDDD SAQDS
Length:525
Mass (Da):56,728
Last modified:February 9, 2010 - v3
Checksum:i9994B7554B2DA2C2
GO

Sequence cautioni

The sequence AAB80652 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AY080868 differs from that shown. Reason: Frameshift at position 504.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ820118 mRNA Translation: ACO38702.1
AC002332 Genomic DNA Translation: AAB80652.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC08817.1
CP002685 Genomic DNA Translation: AEC08818.1
AY080868 mRNA No translation available.
PIRiC84744
RefSeqiNP_850206.4, NM_179875.7 [O22785-1]
NP_850207.4, NM_179876.5 [O22785-1]
UniGeneiAt.44022

Genome annotation databases

EnsemblPlantsiAT2G33340.1; AT2G33340.1; AT2G33340 [O22785-1]
AT2G33340.2; AT2G33340.2; AT2G33340 [O22785-1]
GeneIDi817897
GrameneiAT2G33340.1; AT2G33340.1; AT2G33340 [O22785-1]
AT2G33340.2; AT2G33340.2; AT2G33340 [O22785-1]
KEGGiath:AT2G33340

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPR19B_ARATH
AccessioniPrimary (citable) accession number: O22785
Secondary accession number(s): C1KE07, Q3EBP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 9, 2010
Last modified: May 23, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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