Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA-processing factor 19 homolog 2

Gene

PRP19B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ubiquitin-protein ligase which is mainly involved pre-mRNA splicing and DNA repair (By similarity). Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity.By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • DNA repair Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • mRNA processing Source: UniProtKB-KW
  • protein K63-linked ubiquitination Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Immunity, Innate immunity, mRNA processing, mRNA splicing, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-72163. mRNA Splicing - Major Pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 19 homolog 2Curated (EC:6.3.2.-1 Publication)
Alternative name(s):
MOS4-associated complex protein 3B
Short name:
MAC protein 3B
Plant U-box protein 60
U-box domain-containing protein 60
Gene namesi
Name:PRP19B
Synonyms:MAC3B, PUB60
Ordered Locus Names:At2g33340
ORF Names:F4P9.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G33340.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • Cul4-RING E3 ubiquitin ligase complex Source: TAIR
  • nucleus Source: UniProtKB
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Pre-mRNA-processing factor 19 homolog 2PRO_0000322138Add
BLAST

Proteomic databases

PaxDbiO22785.
PRIDEiO22785.

PTM databases

iPTMnetiO22785.

Expressioni

Gene expression databases

ExpressionAtlasiO22785. baseline and differential.
GenevisibleiO22785. AT.

Interactioni

Subunit structurei

Homotetramer. Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 which is related to the PRP19C/Prp19 complex/NTC/Nineteen complex identified in other organisms. Associated with the spliceosome.By similarity1 Publication

Protein-protein interaction databases

BioGridi3244. 1 interaction.
IntActiO22785. 2 interactions.
STRINGi3702.AT2G33340.1.

Structurei

3D structure databases

ProteinModelPortaliO22785.
SMRiO22785. Positions 1-131, 197-509.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7070U-boxAdd
BLAST
Repeati220 – 26142WD 1Add
BLAST
Repeati262 – 30140WD 2Add
BLAST
Repeati307 – 34640WD 3Add
BLAST
Repeati351 – 39040WD 4Add
BLAST
Repeati393 – 43139WD 5Add
BLAST
Repeati433 – 46937WD 6Add
BLAST
Repeati478 – 51740WD 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi409 – 42416DWD boxAdd
BLAST

Domaini

The DWD box is required for interaction with DDB1A.By similarity

Sequence similaritiesi

Belongs to the WD repeat PRP19 family.Curated
Contains 1 U-box domain.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0289. Eukaryota.
ENOG410XPQV. LUCA.
HOGENOMiHOG000177308.
InParanoidiO22785.
OMAiNCIHTLK.
PhylomeDBiO22785.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR013915. Pre-mRNA_splic_Prp19.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08606. Prp19. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O22785-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNCAISGEVP VEPVVSTKSG LLFERRLIER HISDYGKCPV TGEPLTIDDI
60 70 80 90 100
VPIKTGEIIK PKTLHTASIP GLLGTFQNEW DGLMLSNFAL EQQLHTARQE
110 120 130 140 150
LSHALYQHDS ACRVIARLKK ERDEARQLLA EVERHIPAAP EAVTANAALS
160 170 180 190 200
NGKRAAVDEE LGPDAKKLCP GISAEIITEL TDCNAALSQK RKKRQIPQTL
210 220 230 240 250
ASIDTLERFT QLSSHPLHKT NKPGICSMDI LHSKDVIATG GVDATAVLFD
260 270 280 290 300
RPSGQILSTL TGHSKKVTSV KFVGDSDLVL TASADKTVRI WRNPGDGNYA
310 320 330 340 350
CGYTLNDHSA EVRAVTVHPT NKYFVSASLD GTWCFYDLSS GSCLAQVSDD
360 370 380 390 400
SKNVDYTAAA FHPDGLILGT GTSQSVVKIW DVKSQANVAK FDGHTGEVTA
410 420 430 440 450
ISFSENGYFL ATAAEDGVRL WDLRKLRNFK SFLSADANSV EFDPSGSYLG
460 470 480 490 500
IAASDIKVYQ TASVKAEWNL IKTLPDLSGT GKATCVKFGS DAQYVAVGSM
510 520
DRNLRIFGLP GDEKANVDDD SAQDS
Length:525
Mass (Da):56,728
Last modified:February 9, 2010 - v3
Checksum:i9994B7554B2DA2C2
GO

Sequence cautioni

The sequence AAB80652.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AY080868 differs from that shown. Reason: Frameshift at position 504. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ820118 mRNA. Translation: ACO38702.1.
AC002332 Genomic DNA. Translation: AAB80652.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08817.1.
CP002685 Genomic DNA. Translation: AEC08818.1.
AY080868 mRNA. No translation available.
PIRiC84744.
RefSeqiNP_850206.4. NM_179875.6. [O22785-1]
NP_850207.4. NM_179876.5. [O22785-1]
UniGeneiAt.44022.

Genome annotation databases

EnsemblPlantsiAT2G33340.1; AT2G33340.1; AT2G33340. [O22785-1]
AT2G33340.2; AT2G33340.2; AT2G33340. [O22785-1]
GeneIDi817897.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ820118 mRNA. Translation: ACO38702.1.
AC002332 Genomic DNA. Translation: AAB80652.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC08817.1.
CP002685 Genomic DNA. Translation: AEC08818.1.
AY080868 mRNA. No translation available.
PIRiC84744.
RefSeqiNP_850206.4. NM_179875.6. [O22785-1]
NP_850207.4. NM_179876.5. [O22785-1]
UniGeneiAt.44022.

3D structure databases

ProteinModelPortaliO22785.
SMRiO22785. Positions 1-131, 197-509.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3244. 1 interaction.
IntActiO22785. 2 interactions.
STRINGi3702.AT2G33340.1.

PTM databases

iPTMnetiO22785.

Proteomic databases

PaxDbiO22785.
PRIDEiO22785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G33340.1; AT2G33340.1; AT2G33340. [O22785-1]
AT2G33340.2; AT2G33340.2; AT2G33340. [O22785-1]
GeneIDi817897.

Organism-specific databases

TAIRiAT2G33340.

Phylogenomic databases

eggNOGiKOG0289. Eukaryota.
ENOG410XPQV. LUCA.
HOGENOMiHOG000177308.
InParanoidiO22785.
OMAiNCIHTLK.
PhylomeDBiO22785.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-6781823. Formation of TC-NER Pre-Incision Complex.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiO22785.

Gene expression databases

ExpressionAtlasiO22785. baseline and differential.
GenevisibleiO22785. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR013915. Pre-mRNA_splic_Prp19.
IPR003613. Ubox_domain.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF08606. Prp19. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51698. U_BOX. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two Prp19-like U-box proteins in the MOS4-associated complex play redundant roles in plant innate immunity."
    Monaghan J., Xu F., Gao M., Zhao Q., Palma K., Long C., Chen S., Zhang Y., Li X.
    PLoS Pathog. 5:E1000526-E1000526(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF THE MAC COMPLEX.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Biochemical function of typical and variant Arabidopsis thaliana U-box E3 ubiquitin-protein ligases."
    Wiborg J., O'Shea C., Skriver K.
    Biochem. J. 413:447-457(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  6. "Characterization of Arabidopsis and rice DWD proteins and their roles as substrate receptors for CUL4-RING E3 ubiquitin ligases."
    Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H., He Y.J., Xiong Y., Deng X.W.
    Plant Cell 20:152-167(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DWD MOTIF.

Entry informationi

Entry nameiPR19B_ARATH
AccessioniPrimary (citable) accession number: O22785
Secondary accession number(s): C1KE07, Q3EBP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 9, 2010
Last modified: May 11, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.