O22781 (SUVH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase, H3 lysine-9, H3 lysine-27, H4 lysine-20 and cytosine specific SUVH2
EC=2.1.1.43

Alternative name(s):
Cytosine-HMTase 2
H3-K27-HMTase 2
H4-K20-HMTase 2
Histone H3-K9 methyltransferase 2
Short name=H3-K9-HMTase 2
Protein SET DOMAIN GROUP 3
Suppressor of variegation 3-9 homolog protein 2
Short name=Su(var)3-9 homolog protein 2

Gene names

Name:	SUVH2
Synonyms:	SDG3, SET3
Ordered Locus Names:	At2g33290
ORF Names:	F4P9.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone methyltransferase. Methylates 'Lys-9' and 'Lys-27' of histone H3, 'Lys-20' of H4, and cytosine. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression. Plays a central role in gene silencing. Ref.4 Ref.5
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus. Chromosome › centromere. Note: Associates with centromeric constitutive heterochromatin. Ref.1 Ref.4
Tissue specificity	Expressed at low levels in leaves stems and flowers. Ref.1
Domain	Although both SET and pre-SET domains are present, the absence of the post-SET domain may alter the methyltransferase activity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 pre-SET domain. Contains 1 SET domain. Contains 1 YDG domain.

Ontologies

Keywords
Cellular component	Centromere Chromosome Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of gene expression, epigenetic Inferred from mutant phenotype Ref.4. Source: TAIR
Cellular_component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear heterochromatin Inferred from direct assay Ref.4. Source: TAIR
Molecular_function	histone methyltransferase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Histone-lysine N-methyltransferase, H3 lysine-9, H3 lysine-27, H4 lysine-20 and cytosine specific SUVH2		PRO_0000186073
Regions
	Domain	202 – 358	157	YDG
	Domain	434 – 492	59	Pre-SET
	Domain	494 – 642	149	SET
Experimental info
	Mutagenesis	143	1	E → D in 5-1; ectopic nuclear localization. Ref.4
	Mutagenesis	351	1	R → K in 5-2; loss of 5-methylcytosine, H3K9 dimethylation and H4K20 monomethylation. Ref.4
	Mutagenesis	385	1	V → A in 5-3; loss of 5-methylcytosine, H3K9 dimethylation and H4K20 monomethylation. Ref.4
	Mutagenesis	411	1	R → I in 5-4; loss of H3K9 and H4K20 methylation. Ref.4
	Mutagenesis	584	1	V → G in 5-5; loss of H3K9 and H4K20 methylation. Ref.4
	Mutagenesis	620	1	M → T in 5-6; loss of H3K9 and H4K20 methylation; when associated with T-630. Ref.4
	Mutagenesis	630	1	L → V in 5-6; loss of H3K9 and H4K20 methylation; when associated with V-620. Ref.4
	Mutagenesis	645	1	G → S in 5-7; loss of H3K9 and H4K20 methylation. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O22781 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 412AD76C8869ACF9	FASTA	651	72,848
	10 20 30 40 50 60 MSTLLPFPDL NLMPDSQSST AGTTAGDTVV TGKLEVKSEP IEEWQTPPSS TSDQSANTDL 70 80 90 100 110 120 IAEFIRISEL FRSAFKPLQV KGLDGVSVYG LDSGAIVAVP EKENRELIEP PPGFKDNRVS 130 140 150 160 170 180 TVVVSPKFER PRELARIAIL GHEQRKELRQ VMKRTRMTYE SLRIHLMAES MKNHVLGQGR 190 200 210 220 230 240 RRRSDMAAAY IMRDRGLWLN YDKHIVGPVT GVEVGDIFFY RMELCVLGLH GQTQAGIDCL 250 260 270 280 290 300 TAERSATGEP IATSIVVSGG YEDDEDTGDV LVYTGHGGQD HQHKQCDNQR LVGGNLGMER 310 320 330 340 350 360 SMHYGIEVRV IRGIKYENSI SSKVYVYDGL YKIVDWWFAV GKSGFGVFKF RLVRIEGQPM 370 380 390 400 410 420 MGSAVMRFAQ TLRNKPSMVR PTGYVSFDLS NKKENVPVFL YNDVDGDQEP RHYEYIAKAV 430 440 450 460 470 480 FPPGIFGQGG ISRTGCECKL SCTDDCLCAR KNGGEFAYDD NGHLLKGKHV VFECGEFCTC 490 500 510 520 530 540 GPSCKSRVTQ KGLRNRLEVF RSKETGWGVR TLDLIEAGAF ICEYAGVVVT RLQAEILSMN 550 560 570 580 590 600 GDVMVYPGRF TDQWRNWGDL SQVYPDFVRP NYPSLPPLDF SMDVSRMRNV ACYISHSKEP 610 620 630 640 650 NVMVQFVLHD HNHLMFPRVM LFALENISPL AELSLDYGLA DEVNGKLAIC N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes." Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B. Nucleic Acids Res. 29:4319-4333(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, NOMENCLATURE, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis." Naumann K., Fischer A., Hofmann I., Krauss V., Phalke S., Irmler K., Hause G., Aurich A.-C., Dorn R., Jenuwein T., Reuter G. EMBO J. 24:1418-1429(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-143; ARG-351; VAL-385; ARG-411; VAL-584; MET-620; LEU-630 AND GLY-645.
[5]	"Heterochromatin proteins and the control of heterochromatic gene silencing in Arabidopsis." Fischer A., Hofmann I., Naumann K., Reuter G. J. Plant Physiol. 163:358-368(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF344445 mRNA. Translation: AAK28967.1. AC002332 Genomic DNA. Translation: AAB80647.1. CP002685 Genomic DNA. Translation: AEC08810.1.
IPI	IPI00548715.
PIR	F84743.
RefSeq	NP_180887.1. NM_128889.2.
UniGene	At.12115.
3D structure databases
ProteinModelPortal	O22781.
SMR	O22781. Positions 204-359, 387-637.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G33290.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G33290.1; AT2G33290.1; AT2G33290.
GeneID	817892.
KEGG	ath:AT2G33290.
Organism-specific databases
TAIR	At2g33290.
Phylogenomic databases
eggNOG	COG3440.
HOGENOM	HOG000238382.
InParanoid	O22781.
KO	K11420.
OMA	FEYLARP.
PhylomeDB	O22781.
ProtClustDB	CLSN2685938.
Gene expression databases
Genevestigator	O22781.
GermOnline	AT2G33290. Arabidopsis thaliana.
Family and domain databases
Gene3D	2.30.280.10. 1 hit.
InterPro	IPR025794. Hist-Lys_N-MeTrfase_plant. IPR007728. Pre-SET_dom. IPR003606. Pre-SET_Zn-bd_sub. IPR001214. SET_dom. IPR003105. SRA_YDG. [Graphical view]
Pfam	PF05033. Pre-SET. 1 hit. PF00856. SET. 1 hit. PF02182. YDG_SRA. 1 hit. [Graphical view]
SMART	SM00468. PreSET. 1 hit. SM00317. SET. 1 hit. SM00466. SRA. 1 hit. [Graphical view]
PROSITE	PS50867. PRE_SET. 1 hit. PS50280. SET. 1 hit. PS51015. YDG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUVH2_ARATH

Accession

Primary (citable) accession number: O22781

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 9, 2003
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program