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O22781 (SUVH2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone-lysine N-methyltransferase family member SUVH2
Alternative name(s):
Cytosine-HMTase 2
H3-K27-HMTase 2
H4-K20-HMTase 2
Histone H3-K9 methyltransferase 2
Short name=H3-K9-HMTase 2
Protein SET DOMAIN GROUP 3
Suppressor of variegation 3-9 homolog protein 2
Short name=Su(var)3-9 homolog protein 2
Gene names
Name:SUVH2
Synonyms:SDG3, SET3
Ordered Locus Names:At2g33290
ORF Names:F4P9.6
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length651 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histone methyltransferase family member. According to Ref.4, it is required for normal methylation of 'Lys-9' and 'Lys-27' of histone H3, 'Lys-20' of H4, and cytosine, but Ref.6 see no significant effect on histone methylation when the gene is mutated. According to Ref.6, the protein does not bind S-adenosyl-L-methionine and lacks methyltransferase activity. Instead, it may function downstream of DRM2 in RNA-directed DNA methylation, binding to methylated DNA and recruiting DNA-directed RNA polymerase V to chromatin (Ref.7). Plays a central role in gene silencing. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Interacts with DNA-directed RNA polymerase V subunit NRPE1. Ref.7

Subcellular location

Nucleus. Chromosomecentromere. Note: Associates with centromeric constitutive heterochromatin. Ref.1 Ref.4

Tissue specificity

Expressed at low levels in leaves stems and flowers. Ref.1

Domain

Although both SET and pre-SET domains are present, the absence of the post-SET domain may explain the lack of methyltransferase activity. Besides, the Cys residues in the SET domain that normally bind a zinc ion are not conserved.

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster By similarity.

Sequence similarities

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.

Contains 1 pre-SET domain.

Contains 1 SET domain.

Contains 1 YDG domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 651651Histone-lysine N-methyltransferase family member SUVH2
PRO_0000186073

Regions

Domain202 – 358157YDG
Domain434 – 49259Pre-SET
Domain495 – 638144SET

Sites

Metal binding4361Zinc 1 By similarity
Metal binding4361Zinc 2 By similarity
Metal binding4381Zinc 1 By similarity
Metal binding4421Zinc 1 By similarity
Metal binding4421Zinc 3 By similarity
Metal binding4461Zinc 1 By similarity
Metal binding4481Zinc 2 By similarity
Metal binding4741Zinc 2 By similarity
Metal binding4741Zinc 3 By similarity
Metal binding4781Zinc 2 By similarity
Metal binding4801Zinc 3 By similarity
Metal binding4841Zinc 3 By similarity

Experimental info

Mutagenesis1431E → D in 5-1; ectopic nuclear localization. Ref.4
Mutagenesis3511R → K in 5-2; loss of 5-methylcytosine, H3K9 dimethylation and H4K20 monomethylation. Ref.4
Mutagenesis3851V → A in 5-3; loss of 5-methylcytosine, H3K9 dimethylation and H4K20 monomethylation. Ref.4
Mutagenesis4111R → I in 5-4; loss of H3K9 and H4K20 methylation. Ref.4
Mutagenesis5841V → G in 5-5; loss of H3K9 and H4K20 methylation. Ref.4
Mutagenesis6201M → T in 5-6; loss of H3K9 and H4K20 methylation; when associated with T-630. Ref.4
Mutagenesis6301L → V in 5-6; loss of H3K9 and H4K20 methylation; when associated with V-620. Ref.4
Mutagenesis6451G → S in 5-7; loss of H3K9 and H4K20 methylation. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O22781 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 412AD76C8869ACF9

FASTA65172,848
        10         20         30         40         50         60 
MSTLLPFPDL NLMPDSQSST AGTTAGDTVV TGKLEVKSEP IEEWQTPPSS TSDQSANTDL 

        70         80         90        100        110        120 
IAEFIRISEL FRSAFKPLQV KGLDGVSVYG LDSGAIVAVP EKENRELIEP PPGFKDNRVS 

       130        140        150        160        170        180 
TVVVSPKFER PRELARIAIL GHEQRKELRQ VMKRTRMTYE SLRIHLMAES MKNHVLGQGR 

       190        200        210        220        230        240 
RRRSDMAAAY IMRDRGLWLN YDKHIVGPVT GVEVGDIFFY RMELCVLGLH GQTQAGIDCL 

       250        260        270        280        290        300 
TAERSATGEP IATSIVVSGG YEDDEDTGDV LVYTGHGGQD HQHKQCDNQR LVGGNLGMER 

       310        320        330        340        350        360 
SMHYGIEVRV IRGIKYENSI SSKVYVYDGL YKIVDWWFAV GKSGFGVFKF RLVRIEGQPM 

       370        380        390        400        410        420 
MGSAVMRFAQ TLRNKPSMVR PTGYVSFDLS NKKENVPVFL YNDVDGDQEP RHYEYIAKAV 

       430        440        450        460        470        480 
FPPGIFGQGG ISRTGCECKL SCTDDCLCAR KNGGEFAYDD NGHLLKGKHV VFECGEFCTC 

       490        500        510        520        530        540 
GPSCKSRVTQ KGLRNRLEVF RSKETGWGVR TLDLIEAGAF ICEYAGVVVT RLQAEILSMN 

       550        560        570        580        590        600 
GDVMVYPGRF TDQWRNWGDL SQVYPDFVRP NYPSLPPLDF SMDVSRMRNV ACYISHSKEP 

       610        620        630        640        650 
NVMVQFVLHD HNHLMFPRVM LFALENISPL AELSLDYGLA DEVNGKLAIC N 

« Hide

References

« Hide 'large scale' references
[1]"The Arabidopsis thaliana genome contains at least 29 active genes encoding SET domain proteins that can be assigned to four evolutionarily conserved classes."
Baumbusch L.O., Thorstensen T., Krauss V., Fischer A., Naumann K., Assalkhou R., Schulz I., Reuter G., Aalen R.B.
Nucleic Acids Res. 29:4319-4333(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, NOMENCLATURE, TISSUE SPECIFICITY.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Pivotal role of AtSUVH2 in heterochromatic histone methylation and gene silencing in Arabidopsis."
Naumann K., Fischer A., Hofmann I., Krauss V., Phalke S., Irmler K., Hause G., Aurich A.-C., Dorn R., Jenuwein T., Reuter G.
EMBO J. 24:1418-1429(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLU-143; ARG-351; VAL-385; ARG-411; VAL-584; MET-620; LEU-630 AND GLY-645.
[5]"Heterochromatin proteins and the control of heterochromatic gene silencing in Arabidopsis."
Fischer A., Hofmann I., Naumann K., Reuter G.
J. Plant Physiol. 163:358-368(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, GENE FAMILY.
[6]"SRA-domain proteins required for DRM2-mediated de novo DNA methylation."
Johnson L.M., Law J.A., Khattar A., Henderson I.R., Jacobsen S.E.
PLoS Genet. 4:E1000280-E1000280(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, LACK OF METHYLTRANSFERASE ACTIVITY, LACK OF S-ADENOSYL-L-METHIONINE BINDING.
[7]"SRA- and SET-domain-containing proteins link RNA polymerase V occupancy to DNA methylation."
Johnson L.M., Du J., Hale C.J., Bischof S., Feng S., Chodavarapu R.K., Zhong X., Marson G., Pellegrini M., Segal D.J., Patel D.J., Jacobsen S.E.
Nature 507:124-128(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NRPE1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF344445 mRNA. Translation: AAK28967.1.
AC002332 Genomic DNA. Translation: AAB80647.1.
CP002685 Genomic DNA. Translation: AEC08810.1.
PIRF84743.
RefSeqNP_180887.1. NM_128889.2.
UniGeneAt.12115.

3D structure databases

ProteinModelPortalO22781.
SMRO22781. Positions 142-638.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G33290.1-P.

Proteomic databases

PRIDEO22781.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G33290.1; AT2G33290.1; AT2G33290.
GeneID817892.
KEGGath:AT2G33290.

Organism-specific databases

TAIRAT2G33290.

Phylogenomic databases

eggNOGCOG3440.
HOGENOMHOG000238382.
InParanoidO22781.
KOK11420.
OMAVGKVYFY.
PhylomeDBO22781.

Enzyme and pathway databases

BioCycARA:AT2G33290-MONOMER.

Gene expression databases

GenevestigatorO22781.

Family and domain databases

Gene3D2.30.280.10. 1 hit.
InterProIPR025794. Hist-Lys_N-MeTrfase_plant.
IPR007728. Pre-SET_dom.
IPR003606. Pre-SET_Zn-bd_sub.
IPR015947. PUA-like_domain.
IPR001214. SET_dom.
IPR003105. SRA_YDG.
[Graphical view]
PfamPF05033. Pre-SET. 1 hit.
PF02182. SAD_SRA. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTSM00468. PreSET. 1 hit.
SM00317. SET. 1 hit.
SM00466. SRA. 1 hit.
[Graphical view]
SUPFAMSSF88697. SSF88697. 1 hit.
PROSITEPS50867. PRE_SET. 1 hit.
PS51575. SAM_MT43_SUVAR39_2. 1 hit.
PS50280. SET. 1 hit.
PS51015. YDG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSUVH2_ARATH
AccessionPrimary (citable) accession number: O22781
Entry history
Integrated into UniProtKB/Swiss-Prot: May 9, 2003
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names