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O22769

- NDUV2_ARATH

UniProt

O22769 - NDUV2_ARATH

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

At4g02580

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 3 (09 Nov 2004)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi130 – 1301Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi135 – 1351Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi171 – 1711Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi175 – 1751Iron-sulfur (2Fe-2S)Sequence Analysis

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. NADH dehydrogenase (ubiquinone) activity Source: RefGenome
    3. zinc ion binding Source: TAIR

    GO - Biological processi

    1. mitochondrial electron transport, NADH to ubiquinone Source: RefGenome
    2. response to oxidative stress Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciARA:AT4G02580-MONOMER.
    MetaCyc:AT4G02580-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Gene namesi
    Ordered Locus Names:At4g02580
    ORF Names:T10P11.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 4

    Organism-specific databases

    TAIRiAT4G02580.

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3535MitochondrionSequence AnalysisAdd
    BLAST
    Chaini36 – 255220NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020007Add
    BLAST

    Proteomic databases

    PaxDbiO22769.
    PRIDEiO22769.

    Expressioni

    Gene expression databases

    GenevestigatoriO22769.

    Interactioni

    Subunit structurei

    Complex I is composed of at least 49 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiO22769. 2 interactions.
    STRINGi3702.AT4G02580.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22769.
    SMRiO22769. Positions 49-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 24 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1905.
    HOGENOMiHOG000257748.
    InParanoidiO22769.
    KOiK03943.
    OMAiHIPHIRV.
    PhylomeDBiO22769.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10371. PTHR10371. 1 hit.
    PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
    PROSITEiPS01099. COMPLEX1_24K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O22769-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLARLAAKRL LEIRQVFRQP TSQVTRSLST ALNYHLDSPD NKPDLPWEFS    50
    EANQSKVKEI LSYYPSNYKQ SAVIPLLDLA QQQNGGWLPV SAMNAVAKVI 100
    EVAPIRVYEV ATFYSMFNRA KVGKYHLLVC GTTPCMIRGS RDIESALLDH 150
    LGVKRGEVTK DGLFSVGEME CMGCCVNAPM ITVADYSNGS EGYTYNYFED 200
    VTPEKVVEIV EKLRKGEKPP HGTQNPKRIK CGPEGGNKTL LGEPKPPQFR 250
    DLDAC 255
    Length:255
    Mass (Da):28,389
    Last modified:November 9, 2004 - v3
    Checksum:iBD61E8FEF47FE981
    GO

    Sequence cautioni

    The sequence AAC78260.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAB80751.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002330 Genomic DNA. Translation: AAC78260.1. Sequence problems.
    AL161494 Genomic DNA. Translation: CAB80751.1. Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82198.1.
    AY052326 mRNA. Translation: AAK96519.1.
    BT001140 mRNA. Translation: AAN64531.1.
    PIRiT01091.
    RefSeqiNP_567244.1. NM_116492.3.
    UniGeneiAt.22487.

    Genome annotation databases

    EnsemblPlantsiAT4G02580.1; AT4G02580.1; AT4G02580.
    GeneIDi828130.
    KEGGiath:AT4G02580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002330 Genomic DNA. Translation: AAC78260.1 . Sequence problems.
    AL161494 Genomic DNA. Translation: CAB80751.1 . Sequence problems.
    CP002687 Genomic DNA. Translation: AEE82198.1 .
    AY052326 mRNA. Translation: AAK96519.1 .
    BT001140 mRNA. Translation: AAN64531.1 .
    PIRi T01091.
    RefSeqi NP_567244.1. NM_116492.3.
    UniGenei At.22487.

    3D structure databases

    ProteinModelPortali O22769.
    SMRi O22769. Positions 49-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O22769. 2 interactions.
    STRINGi 3702.AT4G02580.1-P.

    Protein family/group databases

    TCDBi 3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Proteomic databases

    PaxDbi O22769.
    PRIDEi O22769.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT4G02580.1 ; AT4G02580.1 ; AT4G02580 .
    GeneIDi 828130.
    KEGGi ath:AT4G02580.

    Organism-specific databases

    GeneFarmi 1820.
    TAIRi AT4G02580.

    Phylogenomic databases

    eggNOGi COG1905.
    HOGENOMi HOG000257748.
    InParanoidi O22769.
    KOi K03943.
    OMAi HIPHIRV.
    PhylomeDBi O22769.

    Enzyme and pathway databases

    BioCyci ARA:AT4G02580-MONOMER.
    MetaCyc:AT4G02580-MONOMER.

    Miscellaneous databases

    PROi O22769.

    Gene expression databases

    Genevestigatori O22769.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10371. PTHR10371. 1 hit.
    PIRSFi PIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
    PROSITEi PS01099. COMPLEX1_24K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
      Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
      , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
      Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.

    Entry informationi

    Entry nameiNDUV2_ARATH
    AccessioniPrimary (citable) accession number: O22769
    Secondary accession number(s): Q940Z9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 9, 2004
    Last modified: October 1, 2014
    This is version 136 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3