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Protein

AP-1 complex subunit mu-2

Gene

AP1M2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting at the trans-Golgi network and early endosomes (TGN/EE). The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Required for KNOLLE localization at the cell plate to mediate cytokinesis. Functions redundantly with AP1M1 in multiple post-Golgi trafficking pathways leading from the TGN to the vacuole, the plasma membrane, and the cell-division plane.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-2
Alternative name(s):
Adaptor protein complex AP-1 subunit mu-2
Adaptor protein-1 mu-adaptin 2
Adaptor-related protein complex 1 subunit mu-2
At-muB2-Ad
Clathrin assembly protein complex 1 mu-2 medium chain
Mu1-adaptin 2
Protein HAPLESS 13
Gene namesi
Name:AP1M2
Synonyms:HAP13
Ordered Locus Names:At1g60780
ORF Names:F8A5.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G60780.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Short pollen tube growth and failure to exit the style (PubMed:15514068). Compromised cytokinesis due to the mislocalization of the KNOLLE syntaxin (PubMed:23543752, PubMed:23733933). Full spectrum of growth defects, suggestive of compromised auxin signaling and of defective RLK signaling. Cell morphogenesis was also disturbed (PubMed:23766365). Impaired pollen function and growth retardation phenotype (PubMed:23733933).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428AP-1 complex subunit mu-2PRO_0000424260Add
BLAST

Proteomic databases

PaxDbiO22715.
PRIDEiO22715.
ProMEXiO22715.

PTM databases

iPTMnetiO22715.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

GenevisibleiO22715. AT.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit and beta-type subunit), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).

Protein-protein interaction databases

BioGridi27596. 2 interactions.
STRINGi3702.AT1G60780.1.

Structurei

3D structure databases

ProteinModelPortaliO22715.
SMRiO22715. Positions 4-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini170 – 426257MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
HOGENOMiHOG000173247.
InParanoidiO22715.
KOiK12393.
OMAiAQIESHS.
PhylomeDBiO22715.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAASALFL LDIKGRVLVW RDYRGDVSAA QAERFFTKLI EKEGDSQSND
60 70 80 90 100
PVAYDNGVTY MFVQHSNVYL MIASRQNCNA ASLLFFLHRV VDVFKHYFEE
110 120 130 140 150
LEEESLRDNF VVVYELLDEM MDFGYPQYTE ARILSEFIKT DAYRMEVTQR
160 170 180 190 200
PPMAVTNAVS WRSEGIQYKK NEVFLDVIEN VNILVNSNGQ IVRSDVVGAL
210 220 230 240 250
KMRTYLTGMP ECKLGLNDRV LLEAQGRATK GKAIDLEDIK FHQCVRLARF
260 270 280 290 300
ENDRTISFIP PDGAFDLMTY RLSTQVKPLI WVEAQIESHS RSRVEMLIKA
310 320 330 340 350
RSQFKERSTA TNVEIELPVP TDASNPTVRT SLGSASYAPE KDALVWKIKS
360 370 380 390 400
FPGNKEYMLR AEFHLPSITA EEATPERKAP IRVKFEIPYF TVSGIQVRYL
410 420
KIIEKSGYQA LPWVRYITMA GEYELRLV
Length:428
Mass (Da):49,032
Last modified:January 1, 1998 - v1
Checksum:i1C3C18CF06919C4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002292 Genomic DNA. Translation: AAB71967.1.
CP002684 Genomic DNA. Translation: AEE33731.1.
AY099652 mRNA. Translation: AAM20503.1.
BT002144 mRNA. Translation: AAN72155.1.
PIRiC96633.
RefSeqiNP_176277.1. NM_104761.3.
UniGeneiAt.36589.

Genome annotation databases

EnsemblPlantsiAT1G60780.1; AT1G60780.1; AT1G60780.
GeneIDi842372.
GrameneiAT1G60780.1; AT1G60780.1; AT1G60780.
KEGGiath:AT1G60780.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002292 Genomic DNA. Translation: AAB71967.1.
CP002684 Genomic DNA. Translation: AEE33731.1.
AY099652 mRNA. Translation: AAM20503.1.
BT002144 mRNA. Translation: AAN72155.1.
PIRiC96633.
RefSeqiNP_176277.1. NM_104761.3.
UniGeneiAt.36589.

3D structure databases

ProteinModelPortaliO22715.
SMRiO22715. Positions 4-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27596. 2 interactions.
STRINGi3702.AT1G60780.1.

PTM databases

iPTMnetiO22715.

Proteomic databases

PaxDbiO22715.
PRIDEiO22715.
ProMEXiO22715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G60780.1; AT1G60780.1; AT1G60780.
GeneIDi842372.
GrameneiAT1G60780.1; AT1G60780.1; AT1G60780.
KEGGiath:AT1G60780.

Organism-specific databases

TAIRiAT1G60780.

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
HOGENOMiHOG000173247.
InParanoidiO22715.
KOiK12393.
OMAiAQIESHS.
PhylomeDBiO22715.

Miscellaneous databases

PROiO22715.

Gene expression databases

GenevisibleiO22715. AT.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, REVIEW.
  5. "Arabidopsis hapless mutations define essential gametophytic functions."
    Johnson M.A., von Besser K., Zhou Q., Smith E., Aux G., Patton D., Levin J.Z., Preuss D.
    Genetics 168:971-982(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Arabidopsis muA-adaptin interacts with the tyrosine motif of the vacuolar sorting receptor VSR-PS1."
    Happel N., Hoening S., Neuhaus J.M., Paris N., Robinson D.G., Holstein S.E.
    Plant J. 37:678-693(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "The AP-1 mu adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis."
    Teh O.K., Shimono Y., Shirakawa M., Fukao Y., Tamura K., Shimada T., Hara-Nishimura I.
    Plant Cell Physiol. 54:838-847(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF THE AP-1 COMPLEX.
  8. "HAPLESS13, the Arabidopsis mu1 adaptin, is essential for protein sorting at the trans-Golgi network/early endosome."
    Wang J.G., Li S., Zhao X.Y., Zhou L.Z., Huang G.Q., Feng C., Zhang Y.
    Plant Physiol. 162:1897-1910(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF THE AP-1 COMPLEX.
  9. "Arabidopsis mu-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth."
    Park M., Song K., Reichardt I., Kim H., Mayer U., Stierhof Y.D., Hwang I., Juergens G.
    Proc. Natl. Acad. Sci. U.S.A. 110:10318-10323(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, COMPONENT OF THE AP-1 COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAP1M2_ARATH
AccessioniPrimary (citable) accession number: O22715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.