Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

AP-1 complex subunit mu-2

Gene

AP1M2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting at the trans-Golgi network and early endosomes (TGN/EE). The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Required for KNOLLE localization at the cell plate to mediate cytokinesis. Functions redundantly with AP1M1 in multiple post-Golgi trafficking pathways leading from the TGN to the vacuole, the plasma membrane, and the cell-division plane.3 Publications

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-2
Alternative name(s):
Adaptor protein complex AP-1 subunit mu-2
Adaptor protein-1 mu-adaptin 2
Adaptor-related protein complex 1 subunit mu-2
At-muB2-Ad
Clathrin assembly protein complex 1 mu-2 medium chain
Mu1-adaptin 2
Protein HAPLESS 13
Gene namesi
Name:AP1M2
Synonyms:HAP13
Ordered Locus Names:At1g60780
ORF Names:F8A5.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G60780
TAIRilocus:2036606 AT1G60780

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Short pollen tube growth and failure to exit the style (PubMed:15514068). Compromised cytokinesis due to the mislocalization of the KNOLLE syntaxin (PubMed:23543752, PubMed:23733933). Full spectrum of growth defects, suggestive of compromised auxin signaling and of defective RLK signaling. Cell morphogenesis was also disturbed (PubMed:23766365). Impaired pollen function and growth retardation phenotype (PubMed:23733933).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004242601 – 428AP-1 complex subunit mu-2Add BLAST428

Proteomic databases

PaxDbiO22715
ProMEXiO22715

PTM databases

iPTMnetiO22715

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

ExpressionAtlasiO22715 baseline and differential
GenevisibleiO22715 AT

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit and beta-type subunit), a medium adaptin (mu-type subunit) and a small adaptin (sigma-type subunit).

Protein-protein interaction databases

BioGridi27596, 2 interactors
STRINGi3702.AT1G60780.1

Structurei

3D structure databases

ProteinModelPortaliO22715
SMRiO22715
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini170 – 426MHDPROSITE-ProRule annotationAdd BLAST257

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0937 Eukaryota
ENOG410XPFS LUCA
HOGENOMiHOG000173247
InParanoidiO22715
KOiK12393
OMAiQQVTPCF
OrthoDBiEOG093609LA
PhylomeDBiO22715

Family and domain databases

InterProiView protein in InterPro
IPR036168 AP2_Mu_C_sf
IPR022775 AP_mu_sigma_su
IPR001392 Clathrin_mu
IPR018240 Clathrin_mu_CS
IPR011012 Longin-like_dom_sf
IPR028565 MHD
PfamiView protein in Pfam
PF00928 Adap_comp_sub, 1 hit
PF01217 Clat_adaptor_s, 1 hit
PIRSFiPIRSF005992 Clathrin_mu, 1 hit
PRINTSiPR00314 CLATHRINADPT
SUPFAMiSSF49447 SSF49447, 1 hit
SSF64356 SSF64356, 1 hit
PROSITEiView protein in PROSITE
PS00990 CLAT_ADAPTOR_M_1, 1 hit
PS00991 CLAT_ADAPTOR_M_2, 1 hit
PS51072 MHD, 1 hit

Sequencei

Sequence statusi: Complete.

O22715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGAASALFL LDIKGRVLVW RDYRGDVSAA QAERFFTKLI EKEGDSQSND
60 70 80 90 100
PVAYDNGVTY MFVQHSNVYL MIASRQNCNA ASLLFFLHRV VDVFKHYFEE
110 120 130 140 150
LEEESLRDNF VVVYELLDEM MDFGYPQYTE ARILSEFIKT DAYRMEVTQR
160 170 180 190 200
PPMAVTNAVS WRSEGIQYKK NEVFLDVIEN VNILVNSNGQ IVRSDVVGAL
210 220 230 240 250
KMRTYLTGMP ECKLGLNDRV LLEAQGRATK GKAIDLEDIK FHQCVRLARF
260 270 280 290 300
ENDRTISFIP PDGAFDLMTY RLSTQVKPLI WVEAQIESHS RSRVEMLIKA
310 320 330 340 350
RSQFKERSTA TNVEIELPVP TDASNPTVRT SLGSASYAPE KDALVWKIKS
360 370 380 390 400
FPGNKEYMLR AEFHLPSITA EEATPERKAP IRVKFEIPYF TVSGIQVRYL
410 420
KIIEKSGYQA LPWVRYITMA GEYELRLV
Length:428
Mass (Da):49,032
Last modified:January 1, 1998 - v1
Checksum:i1C3C18CF06919C4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002292 Genomic DNA Translation: AAB71967.1
CP002684 Genomic DNA Translation: AEE33731.1
AY099652 mRNA Translation: AAM20503.1
BT002144 mRNA Translation: AAN72155.1
PIRiC96633
RefSeqiNP_176277.1, NM_104761.4
UniGeneiAt.36589

Genome annotation databases

EnsemblPlantsiAT1G60780.1; AT1G60780.1; AT1G60780
GeneIDi842372
GrameneiAT1G60780.1; AT1G60780.1; AT1G60780
KEGGiath:AT1G60780

Similar proteinsi

Entry informationi

Entry nameiAP1M2_ARATH
AccessioniPrimary (citable) accession number: O22715
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health