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Protein

Probable S-sulfocysteine synthase, chloroplastic

Gene

At3g03630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-sulfocysteine synthase that plays an important role in chloroplast function and are essential for light-dependent redox regulation within the chloroplast. Probably unable to interact with SAT and to form the decameric Cys synthase complex (CSC) and is therefore not an enzymatically true OASTL protein.3 Publications

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication

Cofactori

Kineticsi

  1. KM=0.46 mM for O3-acetyl-L-serine for S-sulfocysteine synthase activity1 Publication
  2. KM=0.93 mM for thiosulfate for S-sulfocysteine synthase activity1 Publication
  1. Vmax=20.7 mmol/min/mg enzyme toward thiosulfate for S-sulfocysteine synthase activity1 Publication

pH dependencei

Optimum pH is 8-9.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171Pyridoxal phosphateBy similarity1
Binding sitei353Pyridoxal phosphateBy similarity1

GO - Molecular functioni

  • cysteine synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • cysteine biosynthetic process from serine Source: GO_Central
  • photosynthesis Source: TAIR
  • photosynthetic acclimation Source: TAIR
  • regulation of hydrogen peroxide metabolic process Source: TAIR
  • regulation of superoxide metabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G03630-MONOMER
MetaCyc:AT3G03630-MONOMER
ReactomeiR-ATH-1614603 Cysteine formation from homocysteine

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-sulfocysteine synthase, chloroplastic (EC:2.5.1.47)
Alternative name(s):
Beta-substituted Ala synthase 5;1
Short name:
ARAth-Bsas5;1
CS26
O-acetylserine sulfhydrylase
Gene namesi
Ordered Locus Names:At3g03630
ORF Names:T12J13.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G03630
TAIRilocus:2096454 AT3G03630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:18024555). Exhibits a reduction in size and shows paleness, but penetrance of the growth phenotype depended on the light regime (PubMed:20179139).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 84ThylakoidSequence analysis
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000000635485 – 404Probable S-sulfocysteine synthase, chloroplasticAdd BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei140N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiO22682
PRIDEiO22682

Expressioni

Gene expression databases

ExpressionAtlasiO22682 baseline and differential
GenevisibleiO22682 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi6536, 1 interactor
IntActiO22682, 1 interactor
STRINGi3702.AT3G03630.1

Structurei

3D structure databases

ProteinModelPortaliO22682
SMRiO22682
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni275 – 279Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1252 Eukaryota
COG0031 LUCA
HOGENOMiHOG000217394
InParanoidiO22682
KOiK01738
OMAiFRPIYDS
OrthoDBiEOG09360FNG
PhylomeDBiO22682

Family and domain databases

InterProiView protein in InterPro
IPR005856 Cys_synth
IPR005859 CysK
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01139 cysK, 1 hit
TIGR01136 cysKM, 1 hit
PROSITEiView protein in PROSITE
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFASPSLRL LPQSPLGRIT SKLHRFSTAK LSLFSFHHDS SSSLAVRTPV
60 70 80 90 100
SSFVVGAISG KSSTGTKSKS KTKRKPPPPP PVTTVAEEQH IAESETVNIA
110 120 130 140 150
EDVTQLIGST PMVYLNRVTD GCLADIAAKL ESMEPCRSVK DRIGLSMINE
160 170 180 190 200
AENSGAITPR KTVLVEPTTG NTGLGIAFVA AAKGYKLIVT MPASINIERR
210 220 230 240 250
MLLRALGAEI VLTNPEKGLK GAVDKAKEIV LKTKNAYMFQ QFDNTANTKI
260 270 280 290 300
HFETTGPEIW EDTMGNVDIF VAGIGTGGTV TGTGGFLKMM NKDIKVVGVE
310 320 330 340 350
PSERSVISGD NPGYLPGILD VKLLDEVFKV SNGEAIEMAR RLALEEGLLV
360 370 380 390 400
GISSGAAAVA AVSLAKRAEN AGKLITVLFP SHGERYITTA LFSSINREVQ

EMRY
Length:404
Mass (Da):43,161
Last modified:January 1, 1998 - v1
Checksum:i50C813C470B1CF64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003041 mRNA Translation: BAA21628.1
AC009327 Genomic DNA Translation: AAF03469.1
CP002686 Genomic DNA Translation: AEE73965.1
AY099573 mRNA Translation: AAM20425.1
BT002155 mRNA Translation: AAN72166.1
RefSeqiNP_187013.1, NM_111234.5
UniGeneiAt.20912

Genome annotation databases

EnsemblPlantsiAT3G03630.1; AT3G03630.1; AT3G03630
GeneIDi821203
GrameneiAT3G03630.1; AT3G03630.1; AT3G03630
KEGGiath:AT3G03630

Similar proteinsi

Entry informationi

Entry nameiCYSK4_ARATH
AccessioniPrimary (citable) accession number: O22682
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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