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Protein

Probable S-sulfocysteine synthase, chloroplastic

Gene

At3g03630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

S-sulfocysteine synthase that plays an important role in chloroplast function and are essential for light-dependent redox regulation within the chloroplast. Probably unable to interact with SAT and to form the decameric Cys synthase complex (CSC) and is therefore not an enzymatically true OASTL protein.3 Publications

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.1 Publication

Cofactori

Kineticsi

  1. KM=0.46 mM for O(3)-acetyl-L-serine for S-sulfocysteine synthase activity1 Publication
  2. KM=0.93 mM for thiosulfate for S-sulfocysteine synthase activity1 Publication
  1. Vmax=20.7 mmol/min/mg enzyme toward thiosulfate for S-sulfocysteine synthase activity1 Publication

pH dependencei

Optimum pH is 8-9.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711Pyridoxal phosphateBy similarity
Binding sitei353 – 3531Pyridoxal phosphateBy similarity

GO - Molecular functioni

  • cysteine synthase activity Source: TAIR
  • pyridoxal phosphate binding Source: GO_Central
  • transferase activity Source: UniProtKB-KW

GO - Biological processi

  • cysteine biosynthetic process from serine Source: GO_Central
  • photosynthesis Source: TAIR
  • photosynthetic acclimation Source: TAIR
  • regulation of hydrogen peroxide metabolic process Source: TAIR
  • regulation of superoxide metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G03630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable S-sulfocysteine synthase, chloroplastic (EC:2.5.1.47)
Alternative name(s):
Beta-substituted Ala synthase 5;1
Short name:
ARAth-Bsas5;1
CS26
O-acetylserine sulfhydrylase
Gene namesi
Ordered Locus Names:At3g03630
ORF Names:T12J13.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G03630.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid lumen Source: UniProtKB-SubCell
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype (PubMed:18024555). Exhibits a reduction in size and shows paleness, but penetrance of the growth phenotype depended on the light regime (PubMed:20179139).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei? – 84ThylakoidSequence analysis
Transit peptidei1 – ?ChloroplastSequence analysis
Chaini85 – 404320Probable S-sulfocysteine synthase, chloroplasticPRO_0000006354Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei140 – 1401N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiO22682.
PRIDEiO22682.

Expressioni

Gene expression databases

GenevisibleiO22682. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi6536. 1 interaction.
IntActiO22682. 1 interaction.
STRINGi3702.AT3G03630.1.

Structurei

3D structure databases

ProteinModelPortaliO22682.
SMRiO22682. Positions 97-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni275 – 2795Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiO22682.
KOiK01738.
OMAiHGERYIT.
OrthoDBiEOG09360FNG.
PhylomeDBiO22682.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFASPSLRL LPQSPLGRIT SKLHRFSTAK LSLFSFHHDS SSSLAVRTPV
60 70 80 90 100
SSFVVGAISG KSSTGTKSKS KTKRKPPPPP PVTTVAEEQH IAESETVNIA
110 120 130 140 150
EDVTQLIGST PMVYLNRVTD GCLADIAAKL ESMEPCRSVK DRIGLSMINE
160 170 180 190 200
AENSGAITPR KTVLVEPTTG NTGLGIAFVA AAKGYKLIVT MPASINIERR
210 220 230 240 250
MLLRALGAEI VLTNPEKGLK GAVDKAKEIV LKTKNAYMFQ QFDNTANTKI
260 270 280 290 300
HFETTGPEIW EDTMGNVDIF VAGIGTGGTV TGTGGFLKMM NKDIKVVGVE
310 320 330 340 350
PSERSVISGD NPGYLPGILD VKLLDEVFKV SNGEAIEMAR RLALEEGLLV
360 370 380 390 400
GISSGAAAVA AVSLAKRAEN AGKLITVLFP SHGERYITTA LFSSINREVQ

EMRY
Length:404
Mass (Da):43,161
Last modified:January 1, 1998 - v1
Checksum:i50C813C470B1CF64
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003041 mRNA. Translation: BAA21628.1.
AC009327 Genomic DNA. Translation: AAF03469.1.
CP002686 Genomic DNA. Translation: AEE73965.1.
AY099573 mRNA. Translation: AAM20425.1.
BT002155 mRNA. Translation: AAN72166.1.
RefSeqiNP_187013.1. NM_111234.4.
UniGeneiAt.20912.

Genome annotation databases

EnsemblPlantsiAT3G03630.1; AT3G03630.1; AT3G03630.
GeneIDi821203.
GrameneiAT3G03630.1; AT3G03630.1; AT3G03630.
KEGGiath:AT3G03630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003041 mRNA. Translation: BAA21628.1.
AC009327 Genomic DNA. Translation: AAF03469.1.
CP002686 Genomic DNA. Translation: AEE73965.1.
AY099573 mRNA. Translation: AAM20425.1.
BT002155 mRNA. Translation: AAN72166.1.
RefSeqiNP_187013.1. NM_111234.4.
UniGeneiAt.20912.

3D structure databases

ProteinModelPortaliO22682.
SMRiO22682. Positions 97-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6536. 1 interaction.
IntActiO22682. 1 interaction.
STRINGi3702.AT3G03630.1.

Proteomic databases

PaxDbiO22682.
PRIDEiO22682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G03630.1; AT3G03630.1; AT3G03630.
GeneIDi821203.
GrameneiAT3G03630.1; AT3G03630.1; AT3G03630.
KEGGiath:AT3G03630.

Organism-specific databases

TAIRiAT3G03630.

Phylogenomic databases

eggNOGiKOG1252. Eukaryota.
COG0031. LUCA.
HOGENOMiHOG000217394.
InParanoidiO22682.
KOiK01738.
OMAiHGERYIT.
OrthoDBiEOG09360FNG.
PhylomeDBiO22682.

Enzyme and pathway databases

BioCyciARA:AT3G03630-MONOMER.

Miscellaneous databases

PROiO22682.

Gene expression databases

GenevisibleiO22682. AT.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSK4_ARATH
AccessioniPrimary (citable) accession number: O22682
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.