Reviewed, UniProtKB/Swiss-Prot O22682 (CYSK4_ARATH)

Names and origin

Protein names

Recommended name:
    Probable cysteine synthase, chloroplastic
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL
    CS26

Gene names

Ordered Locus Names:	At3g03630
ORF Names:	T12J13.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 404		Probable cysteine synthase, chloroplastic		PRO_0000006354
Regions
	Region	275 – 279	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	171	1	Pyridoxal phosphate By similarity
	Binding site	353	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	140	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22682-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 50C813C470B1CF64	FASTA	404	43,161
	10 20 30 40 50 60 MAFASPSLRL LPQSPLGRIT SKLHRFSTAK LSLFSFHHDS SSSLAVRTPV SSFVVGAISG 70 80 90 100 110 120 KSSTGTKSKS KTKRKPPPPP PVTTVAEEQH IAESETVNIA EDVTQLIGST PMVYLNRVTD 130 140 150 160 170 180 GCLADIAAKL ESMEPCRSVK DRIGLSMINE AENSGAITPR KTVLVEPTTG NTGLGIAFVA 190 200 210 220 230 240 AAKGYKLIVT MPASINIERR MLLRALGAEI VLTNPEKGLK GAVDKAKEIV LKTKNAYMFQ 250 260 270 280 290 300 QFDNTANTKI HFETTGPEIW EDTMGNVDIF VAGIGTGGTV TGTGGFLKMM NKDIKVVGVE 310 320 330 340 350 360 PSERSVISGD NPGYLPGILD VKLLDEVFKV SNGEAIEMAR RLALEEGLLV GISSGAAAVA 370 380 390 400 AVSLAKRAEN AGKLITVLFP SHGERYITTA LFSSINREVQ EMRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel cysteine synthase cDNA from Arabidopsis thaliana." Nakamura T., Koizumi N., Sano H. Plant Gene Register PGR97-081 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003041 mRNA. Translation: BAA21628.1. AC009327 Genomic DNA. Translation: AAF03469.1. AY099573 mRNA. Translation: AAM20425.1. BT002155 mRNA. Translation: AAN72166.1.
IPI	IPI00528856.
RefSeq	NP_187013.1.
UniGene	At.20912
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
ModBase	Search...
Protein-protein interaction databases
STRING	O22682.
Proteomic databases
PRIDE	O22682.
Genome annotation databases
GeneID	821203.
GenomeReviews	Gene locus AT3G03630 in contig BA000014_GR.
KEGG	ath:AT3G03630.
NMPDR	fig|3702.1.peg.12315.
Organism-specific databases
TAIR	At3g03630.
Phylogenomic databases
OMA	NAYMFQQ.
Enzyme and pathway databases
BRENDA	2.5.1.47. 302.
Gene expression databases
ArrayExpress	O22682.
Genevestigator	O22682.
GermOnline	AT3G03630. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK4_ARATH

Accession

Primary (citable) accession number: O22682

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)