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O22666

- RGP3_ARATH

UniProt

O22666 - RGP3_ARATH

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Protein

UDP-arabinopyranose mutase 3

Gene

RGP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Is probably active as heteromer in vivo.1 Publication

Catalytic activityi

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.

Cofactori

Mn2+By similarity, Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei154 – 1541Required for activityBy similarity
Sitei161 – 1611Required for activityBy similarity

GO - Molecular functioni

  1. intramolecular transferase activity Source: UniProtKB
  2. UDP-arabinopyranose mutase activity Source: TAIR

GO - Biological processi

  1. cellulose biosynthetic process Source: InterPro
  2. cell wall organization Source: UniProtKB-KW
  3. UDP-L-arabinose metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G08900-MONOMER.

Protein family/group databases

CAZyiGT75. Glycosyltransferase Family 75.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-arabinopyranose mutase 3 (EC:5.4.99.30)
Alternative name(s):
Reversibly glycosylated polypeptide 3
Short name:
AtRGP3
UDP-L-arabinose mutase 3
Gene namesi
Name:RGP3
Ordered Locus Names:At3g08900
ORF Names:T16O11.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G08900.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Golgi apparatus 1 Publication
Note: Soluble and membrane-associated.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. Golgi apparatus Source: UniProtKB
  3. membrane Source: TAIR
  4. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 362361UDP-arabinopyranose mutase 3PRO_0000410986Add
BLAST

Post-translational modificationi

Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO22666.
PRIDEiO22666.

Expressioni

Tissue specificityi

Specifically expressed in developing seeds.1 Publication

Gene expression databases

GenevestigatoriO22666.

Interactioni

Subunit structurei

Heterodimer with RGP1.1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G08900.1-P.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi106 – 1083DXD motif

Domaini

The conserved DXD motif is involved in enzyme activity.By similarity

Sequence similaritiesi

Belongs to the RGP family.Curated

Phylogenomic databases

eggNOGiNOG82578.
HOGENOMiHOG000234443.
InParanoidiO22666.
KOiK13379.
OMAiEEAIPFF.
PhylomeDBiO22666.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamiPF03214. RGP. 1 hit.
[Graphical view]
PIRSFiPIRSF016429. UPTG. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22666-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQLYSSVKP TPMLKDELDI VIPTIRNLDF LEMWRPFFEQ YHLIIVQDGD
60 70 80 90 100
PSKVINIPVG FDYELYNRND INRILGPKAS CISFKDSACR CFGYMVSKKK
110 120 130 140 150
YIYTIDDDCF VAKDPTGKEI NALEQHIKNL LSPSTPHFFN TLYDPYRDGA
160 170 180 190 200
DFVRGYPFSM REGAITAVSH GLWLNIPDYD APTQLVKPLE KNSRYVDAVM
210 220 230 240 250
TIPKGTLFPM CGMNLAFDRE LIGPAMYFGL MGDGQPIGRY DDMWAGWCVK
260 270 280 290 300
VICDHMGWGV KTGLPYIWHS KASNPFVNLK KEYNGIFWQE EAIPFFQSVT
310 320 330 340 350
LPKECTSVQQ CYLELAKLVR EKLGKVDPYF ITLATGMVTW IEAWEELNSA
360
EGTEAEAPKG KN
Length:362
Mass (Da):41,282
Last modified:March 1, 2001 - v2
Checksum:iFCAF64DFA481F4B9
GO

Sequence cautioni

The sequence AAF07834.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAC43271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034255 mRNA. Translation: AAC50002.2.
AC010871 Genomic DNA. Translation: AAF07834.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74693.1.
AK118676 mRNA. Translation: BAC43271.1. Different initiation.
RefSeqiNP_187502.2. NM_111724.2.
UniGeneiAt.19826.

Genome annotation databases

EnsemblPlantsiAT3G08900.1; AT3G08900.1; AT3G08900.
GeneIDi820039.
KEGGiath:AT3G08900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034255 mRNA. Translation: AAC50002.2 .
AC010871 Genomic DNA. Translation: AAF07834.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE74693.1 .
AK118676 mRNA. Translation: BAC43271.1 . Different initiation.
RefSeqi NP_187502.2. NM_111724.2.
UniGenei At.19826.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G08900.1-P.

Protein family/group databases

CAZyi GT75. Glycosyltransferase Family 75.

Proteomic databases

PaxDbi O22666.
PRIDEi O22666.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G08900.1 ; AT3G08900.1 ; AT3G08900 .
GeneIDi 820039.
KEGGi ath:AT3G08900.

Organism-specific databases

TAIRi AT3G08900.

Phylogenomic databases

eggNOGi NOG82578.
HOGENOMi HOG000234443.
InParanoidi O22666.
KOi K13379.
OMAi EEAIPFF.
PhylomeDBi O22666.

Enzyme and pathway databases

BioCyci ARA:AT3G08900-MONOMER.

Gene expression databases

Genevestigatori O22666.

Family and domain databases

InterProi IPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view ]
Pfami PF03214. RGP. 1 hit.
[Graphical view ]
PIRSFi PIRSF016429. UPTG. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis."
    Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V.
    Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-362.
    Strain: cv. Columbia.
  5. "The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis."
    Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V.
    Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiRGP3_ARATH
AccessioniPrimary (citable) accession number: O22666
Secondary accession number(s): Q8GWR6, Q9SR90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3