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O22666 (RGP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-arabinopyranose mutase 3

EC=5.4.99.30
Alternative name(s):
Reversibly glycosylated polypeptide 3
Short name=AtRGP3
UDP-L-arabinose mutase 3
Gene names
Name:RGP3
Ordered Locus Names:At3g08900
ORF Names:T16O11.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Is probably active as heteromer in vivo. Ref.5

Catalytic activity

UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.

Cofactor

Manganese or Magnesium By similarity.

Subunit structure

Heterodimer with RGP1. Ref.5

Subcellular location

Cytoplasmcytosol. Golgi apparatus. Note: Soluble and membrane-associated. Ref.5

Tissue specificity

Specifically expressed in developing seeds. Ref.5

Domain

The conserved DXD motif is involved in enzyme activity By similarity.

Post-translational modification

Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose By similarity.

Sequence similarities

Belongs to the RGP family.

Sequence caution

The sequence AAF07834.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAC43271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 362361UDP-arabinopyranose mutase 3
PRO_0000410986

Regions

Motif106 – 1083DXD motif

Sites

Site1541Required for activity By similarity
Site1611Required for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O22666 [UniParc].

Last modified March 1, 2001. Version 2.
Checksum: FCAF64DFA481F4B9

FASTA36241,282
        10         20         30         40         50         60 
MAQLYSSVKP TPMLKDELDI VIPTIRNLDF LEMWRPFFEQ YHLIIVQDGD PSKVINIPVG 

        70         80         90        100        110        120 
FDYELYNRND INRILGPKAS CISFKDSACR CFGYMVSKKK YIYTIDDDCF VAKDPTGKEI 

       130        140        150        160        170        180 
NALEQHIKNL LSPSTPHFFN TLYDPYRDGA DFVRGYPFSM REGAITAVSH GLWLNIPDYD 

       190        200        210        220        230        240 
APTQLVKPLE KNSRYVDAVM TIPKGTLFPM CGMNLAFDRE LIGPAMYFGL MGDGQPIGRY 

       250        260        270        280        290        300 
DDMWAGWCVK VICDHMGWGV KTGLPYIWHS KASNPFVNLK KEYNGIFWQE EAIPFFQSVT 

       310        320        330        340        350        360 
LPKECTSVQQ CYLELAKLVR EKLGKVDPYF ITLATGMVTW IEAWEELNSA EGTEAEAPKG 


KN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis."
Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V.
Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-362.
Strain: cv. Columbia.
[5]"The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis."
Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V.
Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF034255 mRNA. Translation: AAC50002.2.
AC010871 Genomic DNA. Translation: AAF07834.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74693.1.
AK118676 mRNA. Translation: BAC43271.1. Different initiation.
RefSeqNP_187502.2. NM_111724.2.
UniGeneAt.19826.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G08900.1-P.

Protein family/group databases

CAZyGT75. Glycosyltransferase Family 75.

Proteomic databases

PaxDbO22666.
PRIDEO22666.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G08900.1; AT3G08900.1; AT3G08900.
GeneID820039.
KEGGath:AT3G08900.

Organism-specific databases

TAIRAT3G08900.

Phylogenomic databases

eggNOGNOG82578.
HOGENOMHOG000234443.
InParanoidO22666.
KOK13379.
OMAEEAIPFF.
PhylomeDBO22666.

Enzyme and pathway databases

BioCycARA:AT3G08900-MONOMER.

Gene expression databases

GenevestigatorO22666.

Family and domain databases

InterProIPR029044. Nucleotide-diphossugar_trans.
IPR004901. RGP_fam.
[Graphical view]
PfamPF03214. RGP. 1 hit.
[Graphical view]
PIRSFPIRSF016429. UPTG. 1 hit.
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRGP3_ARATH
AccessionPrimary (citable) accession number: O22666
Secondary accession number(s): Q8GWR6, Q9SR90
Entry history
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names