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O22666

- RGP3_ARATH

UniProt

O22666 - RGP3_ARATH

Protein

UDP-arabinopyranose mutase 3

Gene

RGP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Is probably active as heteromer in vivo.1 Publication

    Catalytic activityi

    UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.

    Cofactori

    Manganese or Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei154 – 1541Required for activityBy similarity
    Sitei161 – 1611Required for activityBy similarity

    GO - Molecular functioni

    1. intramolecular transferase activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. UDP-arabinopyranose mutase activity Source: TAIR

    GO - Biological processi

    1. cellulose biosynthetic process Source: InterPro
    2. UDP-L-arabinose metabolic process Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT3G08900-MONOMER.

    Protein family/group databases

    CAZyiGT75. Glycosyltransferase Family 75.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-arabinopyranose mutase 3 (EC:5.4.99.30)
    Alternative name(s):
    Reversibly glycosylated polypeptide 3
    Short name:
    AtRGP3
    UDP-L-arabinose mutase 3
    Gene namesi
    Name:RGP3
    Ordered Locus Names:At3g08900
    ORF Names:T16O11.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G08900.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Golgi apparatus 1 Publication
    Note: Soluble and membrane-associated.

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. Golgi apparatus Source: UniProtKB
    3. membrane Source: TAIR
    4. plasmodesma Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 362361UDP-arabinopyranose mutase 3PRO_0000410986Add
    BLAST

    Post-translational modificationi

    Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO22666.
    PRIDEiO22666.

    Expressioni

    Tissue specificityi

    Specifically expressed in developing seeds.1 Publication

    Gene expression databases

    GenevestigatoriO22666.

    Interactioni

    Subunit structurei

    Heterodimer with RGP1.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT3G08900.1-P.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi106 – 1083DXD motif

    Domaini

    The conserved DXD motif is involved in enzyme activity.By similarity

    Sequence similaritiesi

    Belongs to the RGP family.Curated

    Phylogenomic databases

    eggNOGiNOG82578.
    HOGENOMiHOG000234443.
    InParanoidiO22666.
    KOiK13379.
    OMAiEEAIPFF.
    PhylomeDBiO22666.

    Family and domain databases

    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR004901. RGP_fam.
    [Graphical view]
    PfamiPF03214. RGP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016429. UPTG. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O22666-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLYSSVKP TPMLKDELDI VIPTIRNLDF LEMWRPFFEQ YHLIIVQDGD    50
    PSKVINIPVG FDYELYNRND INRILGPKAS CISFKDSACR CFGYMVSKKK 100
    YIYTIDDDCF VAKDPTGKEI NALEQHIKNL LSPSTPHFFN TLYDPYRDGA 150
    DFVRGYPFSM REGAITAVSH GLWLNIPDYD APTQLVKPLE KNSRYVDAVM 200
    TIPKGTLFPM CGMNLAFDRE LIGPAMYFGL MGDGQPIGRY DDMWAGWCVK 250
    VICDHMGWGV KTGLPYIWHS KASNPFVNLK KEYNGIFWQE EAIPFFQSVT 300
    LPKECTSVQQ CYLELAKLVR EKLGKVDPYF ITLATGMVTW IEAWEELNSA 350
    EGTEAEAPKG KN 362
    Length:362
    Mass (Da):41,282
    Last modified:March 1, 2001 - v2
    Checksum:iFCAF64DFA481F4B9
    GO

    Sequence cautioni

    The sequence BAC43271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence AAF07834.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF034255 mRNA. Translation: AAC50002.2.
    AC010871 Genomic DNA. Translation: AAF07834.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74693.1.
    AK118676 mRNA. Translation: BAC43271.1. Different initiation.
    RefSeqiNP_187502.2. NM_111724.2.
    UniGeneiAt.19826.

    Genome annotation databases

    EnsemblPlantsiAT3G08900.1; AT3G08900.1; AT3G08900.
    GeneIDi820039.
    KEGGiath:AT3G08900.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF034255 mRNA. Translation: AAC50002.2 .
    AC010871 Genomic DNA. Translation: AAF07834.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE74693.1 .
    AK118676 mRNA. Translation: BAC43271.1 . Different initiation.
    RefSeqi NP_187502.2. NM_111724.2.
    UniGenei At.19826.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G08900.1-P.

    Protein family/group databases

    CAZyi GT75. Glycosyltransferase Family 75.

    Proteomic databases

    PaxDbi O22666.
    PRIDEi O22666.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G08900.1 ; AT3G08900.1 ; AT3G08900 .
    GeneIDi 820039.
    KEGGi ath:AT3G08900.

    Organism-specific databases

    TAIRi AT3G08900.

    Phylogenomic databases

    eggNOGi NOG82578.
    HOGENOMi HOG000234443.
    InParanoidi O22666.
    KOi K13379.
    OMAi EEAIPFF.
    PhylomeDBi O22666.

    Enzyme and pathway databases

    BioCyci ARA:AT3G08900-MONOMER.

    Gene expression databases

    Genevestigatori O22666.

    Family and domain databases

    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR004901. RGP_fam.
    [Graphical view ]
    Pfami PF03214. RGP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016429. UPTG. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis."
      Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V.
      Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-362.
      Strain: cv. Columbia.
    5. "The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis."
      Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V.
      Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRGP3_ARATH
    AccessioniPrimary (citable) accession number: O22666
    Secondary accession number(s): Q8GWR6, Q9SR90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 27, 2011
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3