O22666 (RGP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-arabinopyranose mutase 3
EC=5.4.99.30

Alternative name(s):
Reversibly glycosylated polypeptide 3
Short name=AtRGP3
UDP-L-arabinose mutase 3

Gene names

Name:	RGP3
Ordered Locus Names:	At3g08900
ORF Names:	T16O11.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	UDP-L-arabinose mutase involved in the biosynthesis of cell wall non-cellulosic polysaccharides. Catalyzes the interconvertion of UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf). Preferentially catalyzes the formation of UDP-Arap from UDP-Araf. At thermodynamic equilibrium in vitro the ratio of the pyranose form over the furanose form is 95:5. Is not active on other UDP-sugars (UDP-Gal, UDP-Xyl, UDP-Glc, GDP-Man and GDP-Fuc). Is probably active as heteromer in vivo. Ref.5
Catalytic activity	UDP-beta-L-arabinofuranose = UDP-beta-L-arabinopyranose.
Cofactor	Manganese or Magnesium By similarity.
Subunit structure	Heterodimer with RGP1. Ref.5
Subcellular location	Cytoplasm › cytosol. Golgi apparatus. Note: Soluble and membrane-associated. Ref.5
Tissue specificity	Specifically expressed in developing seeds. Ref.5
Domain	The conserved DXD motif is involved in enzyme activity By similarity.
Post-translational modification	Reversibly glycosylated in vitro by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose By similarity.
Sequence similarities	Belongs to the RGP family.
Sequence caution	The sequence AAF07834.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAC43271.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cytoplasm Golgi apparatus
Molecular function	Isomerase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-L-arabinose metabolic process Inferred from direct assay Ref.5. Source: TAIR cellulose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay Ref.5. Source: UniProtKB cytosol Inferred from direct assay Ref.5. Source: UniProtKB membrane Inferred from sequence or structural similarity Ref.1. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	UDP-arabinopyranose mutase activity Inferred from direct assay Ref.5. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	UDP-arabinopyranose mutase 3		PRO_0000410986
Regions
	Motif	106 – 108	3	DXD motif
Sites
	Site	154	1	Required for activity By similarity
	Site	161	1	Required for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22666 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: FCAF64DFA481F4B9	FASTA	362	41,282
	10 20 30 40 50 60 MAQLYSSVKP TPMLKDELDI VIPTIRNLDF LEMWRPFFEQ YHLIIVQDGD PSKVINIPVG 70 80 90 100 110 120 FDYELYNRND INRILGPKAS CISFKDSACR CFGYMVSKKK YIYTIDDDCF VAKDPTGKEI 130 140 150 160 170 180 NALEQHIKNL LSPSTPHFFN TLYDPYRDGA DFVRGYPFSM REGAITAVSH GLWLNIPDYD 190 200 210 220 230 240 APTQLVKPLE KNSRYVDAVM TIPKGTLFPM CGMNLAFDRE LIGPAMYFGL MGDGQPIGRY 250 260 270 280 290 300 DDMWAGWCVK VICDHMGWGV KTGLPYIWHS KASNPFVNLK KEYNGIFWQE EAIPFFQSVT 310 320 330 340 350 360 LPKECTSVQQ CYLELAKLVR EKLGKVDPYF ITLATGMVTW IEAWEELNSA EGTEAEAPKG KN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of AtRGP1. A reversibly autoglycosylated arabidopsis protein implicated in cell wall biosynthesis." Delgado I.J., Wang Z., de Rocher A., Keegstra K., Raikhel N.V. Plant Physiol. 116:1339-1350(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 144-362. Strain: cv. Columbia.
[5]	"The interconversion of UDP-L-arabinopyranose and UDP-L-arabinofuranose is indispensable for plant development in Arabidopsis." Rautengarten C., Ebert B., Herter T., Petzold C.J., Ishii T., Mukhopadhyay A., Usadel B., Scheller H.V. Plant Cell 23:1373-1390(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034255 mRNA. Translation: AAC50002.2. AC010871 Genomic DNA. Translation: AAF07834.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE74693.1. AK118676 mRNA. Translation: BAC43271.1. Different initiation.
IPI	IPI00541362.
RefSeq	NP_187502.2. NM_111724.2.
UniGene	At.19826.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G08900.1-P.
Protein family/group databases
CAZy	GT75. Glycosyltransferase Family 75.
Proteomic databases
PaxDb	O22666.
PRIDE	O22666.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	820039.
KEGG	ath:AT3G08900.
Organism-specific databases
TAIR	At3g08900.
Phylogenomic databases
eggNOG	NOG82578.
HOGENOM	HOG000234443.
InParanoid	O22666.
KO	K13379.
OMA	FITLATG.
PhylomeDB	O22666.
ProtClustDB	PLN03180.
Enzyme and pathway databases
BioCyc	ARA:AT3G08900-MONOMER.
Gene expression databases
Genevestigator	O22666.
Family and domain databases
InterPro	IPR004901. RGP_fam. [Graphical view]
Pfam	PF03214. RGP. 1 hit. [Graphical view]
PIRSF	PIRSF016429. UPTG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RGP3_ARATH

Accession

Primary (citable) accession number: O22666
Secondary accession number(s): Q8GWR6, Q9SR90

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program