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Protein

Protease Do-like 1, chloroplastic

Gene

DEGP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease that is required at high temperature. May be involved in the degradation of damaged proteins. In vivo, can degrade beta-casein.2 Publications

Enzyme regulationi

Inhibited by phenylmethylsulfonyl fluoride and O-phenanthroline.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173Charge relay systemSequence analysis1
Active sitei203Charge relay systemSequence analysis1
Active sitei282Charge relay systemSequence analysis1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: TAIR
  • serine-type peptidase activity Source: TAIR

GO - Biological processi

  • photosystem II repair Source: TAIR
  • protein catabolic process Source: TAIR
  • response to cytokinin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Protein family/group databases

MEROPSiS01.472.

Names & Taxonomyi

Protein namesi
Recommended name:
Protease Do-like 1, chloroplastic (EC:3.4.21.-)
Alternative name(s):
Protein DEGRADATION OF PERIPLASMIC PROTEINS 1
Short name:
DEGP PROTEASE 1
Gene namesi
Name:DEGP1
Synonyms:DEG1, DEGP
Ordered Locus Names:At3g27925
ORF Names:K16N12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G27925.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: UniProtKB
  • nucleus Source: TAIR
  • thylakoid Source: TAIR
  • thylakoid lumen Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi282S → G: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 105Thylakoid1 Publication
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_0000026940106 – 439Protease Do-like 1, chloroplasticAdd BLAST334

Proteomic databases

PaxDbiO22609.
PRIDEiO22609.

Expressioni

Inductioni

By heat shock.1 Publication

Gene expression databases

GenevisibleiO22609. AT.

Interactioni

Subunit structurei

Interacts with PTAC16 and other potential targets for degradation under high light conditions.1 Publication

Protein-protein interaction databases

BioGridi7746. 13 interactors.
DIPiDIP-56439N.
IntActiO22609. 2 interactors.
STRINGi3702.AT3G27925.1.

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi116 – 128Combined sources13
Helixi129 – 131Combined sources3
Beta strandi132 – 142Combined sources11
Beta strandi144 – 146Combined sources3
Beta strandi149 – 163Combined sources15
Turni164 – 166Combined sources3
Beta strandi167 – 170Combined sources4
Helixi172 – 175Combined sources4
Beta strandi179 – 184Combined sources6
Beta strandi190 – 199Combined sources10
Helixi200 – 202Combined sources3
Beta strandi204 – 208Combined sources5
Helixi213 – 215Combined sources3
Beta strandi232 – 237Combined sources6
Helixi239 – 241Combined sources3
Beta strandi245 – 258Combined sources14
Beta strandi261 – 265Combined sources5
Beta strandi267 – 273Combined sources7
Beta strandi285 – 287Combined sources3
Beta strandi293 – 301Combined sources9
Beta strandi303 – 306Combined sources4
Beta strandi311 – 315Combined sources5
Helixi316 – 329Combined sources14
Beta strandi339 – 341Combined sources3
Turni344 – 351Combined sources8
Beta strandi353 – 359Combined sources7
Beta strandi362 – 364Combined sources3
Helixi365 – 369Combined sources5
Beta strandi378 – 380Combined sources3
Beta strandi397 – 399Combined sources3
Helixi400 – 406Combined sources7
Beta strandi414 – 420Combined sources7
Beta strandi422 – 431Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QO6X-ray2.50A/B/C105-439[»]
ProteinModelPortaliO22609.
SMRiO22609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini326 – 423PDZPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 323Serine proteaseAdd BLAST170

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiO22609.
OMAiAWFSPRE.
OrthoDBiEOG09360EEW.
PhylomeDBiO22609.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTTSCSLL LSSTLFLHSP PSSHLSFFNL SSSRSSPISL YPIRSKRYFR
60 70 80 90 100
ILSKLSLNDN NRDDDDDTLH FTPFSAVKPF FLLCTSVALS FSLFAASPAV
110 120 130 140 150
ESASAFVVST PKKLQTDELA TVRLFQENTP SVVYITNLAV RQDAFTLDVL
160 170 180 190 200
EVPQGSGSGF VWDKQGHIVT NYHVIRGASD LRVTLADQTT FDAKVVGFDQ
210 220 230 240 250
DKDVAVLRID APKNKLRPIP VGVSADLLVG QKVFAIGNPF GLDHTLTTGV
260 270 280 290 300
ISGLRREISS AATGRPIQDV IQTDAAINPG NSGGPLLDSS GTLIGINTAI
310 320 330 340 350
YSPSGASSGV GFSIPVDTVG GIVDQLVRFG KVTRPILGIK FAPDQSVEQL
360 370 380 390 400
GVSGVLVLDA PPSGPAGKAG LQSTKRDGYG RLVLGDIITS VNGTKVSNGS
410 420 430
DLYRILDQCK VGDEVTVEVL RGDHKEKISV TLEPKPDES
Length:439
Mass (Da):46,674
Last modified:August 16, 2004 - v2
Checksum:i05EB437DCE71A251
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 25SSTLF…PSSHL → HSPPSSQLSNST (PubMed:9507020).CuratedAdd BLAST14
Sequence conflicti38I → V in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti56S → P in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti62R → G in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti66D → G in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti70 – 71HF → LL in AAC39436 (PubMed:9507020).Curated2
Sequence conflicti357V → L in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti383V → I in AAC39436 (PubMed:9507020).Curated1
Sequence conflicti418E → Q in AAC39436 (PubMed:9507020).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028842 mRNA. Translation: AAC39436.1.
AP000371, AP001302 Genomic DNA. Translation: BAB02539.1.
CP002686 Genomic DNA. Translation: AEE77381.1.
AY039585 mRNA. Translation: AAK62640.1.
AY113073 mRNA. Translation: AAM47381.1.
RefSeqiNP_189431.2. NM_113709.5.
UniGeneiAt.47511.

Genome annotation databases

EnsemblPlantsiAT3G27925.1; AT3G27925.1; AT3G27925.
GeneIDi822416.
GrameneiAT3G27925.1; AT3G27925.1; AT3G27925.
KEGGiath:AT3G27925.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF028842 mRNA. Translation: AAC39436.1.
AP000371, AP001302 Genomic DNA. Translation: BAB02539.1.
CP002686 Genomic DNA. Translation: AEE77381.1.
AY039585 mRNA. Translation: AAK62640.1.
AY113073 mRNA. Translation: AAM47381.1.
RefSeqiNP_189431.2. NM_113709.5.
UniGeneiAt.47511.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QO6X-ray2.50A/B/C105-439[»]
ProteinModelPortaliO22609.
SMRiO22609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7746. 13 interactors.
DIPiDIP-56439N.
IntActiO22609. 2 interactors.
STRINGi3702.AT3G27925.1.

Protein family/group databases

MEROPSiS01.472.

Proteomic databases

PaxDbiO22609.
PRIDEiO22609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27925.1; AT3G27925.1; AT3G27925.
GeneIDi822416.
GrameneiAT3G27925.1; AT3G27925.1; AT3G27925.
KEGGiath:AT3G27925.

Organism-specific databases

TAIRiAT3G27925.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
HOGENOMiHOG000223641.
InParanoidiO22609.
OMAiAWFSPRE.
OrthoDBiEOG09360EEW.
PhylomeDBiO22609.

Miscellaneous databases

PROiO22609.

Gene expression databases

GenevisibleiO22609. AT.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEGP1_ARATH
AccessioniPrimary (citable) accession number: O22609
Secondary accession number(s): Q94BX6, Q9LK85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.