O22604 (CFI_IPOPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone--flavonone isomerase
Short name=Chalcone isomerase
EC=5.5.1.6

Gene names

Name:	CHI
Synonyms:	CHI-FL1

Organism

Ipomoea purpurea (Common morning glory) (Pharbitis purpurea)

Taxonomic identifier

4121 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Convolvulaceae › Ipomoeeae › Ipomoea

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Chalcone--flavonone isomerase		PRO_0000166432
Sites
	Binding site	50	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Site	108	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22604 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: CB3A4CBEE80955A6	FASTA	241	25,550
	10 20 30 40 50 60 MSAPPCVAEV KVESYVFPAT VKPPGTAKTL ILGGAGARGL NIDGKFVKFT AIGVYLEADA 70 80 90 100 110 120 VPSLAVKWNG KSVEELTDSV QFFRDIVTGP FEKLTRITMI LPLSGKQYSE KVTENCVAFW 130 140 150 160 170 180 KAAGMYGDAE SKAIDKFNDV FSDQMFPPGA SIFFTQSPLG SLTVSFSKDG SMPEIASAVI 190 200 210 220 230 240 ENKPLSEAVL ESIIGSKGVS PEAKQSLAVR LSEMFINGGD AISGKVGVEN DVIPQTVVVS K

References

[1]	"Control of expression patterns of anthocyanin structural genes by two loci in the common morning glory." Tiffin P.L., Miller R.E., Rausher M.D. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Flower bud.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF028238 mRNA. Translation: AAB86474.1.
PIR	T08006.
3D structure databases
ProteinModelPortal	O22604.
SMR	O22604. Positions 7-215.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.50.70.10. 1 hit.
InterPro	IPR016087. Chalcone_isomerase. IPR016088. Chalcone_isomerase_3-sand. [Graphical view]
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
SUPFAM	SSF54626. Chalcone. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CFI_IPOPU

Accession

Primary (citable) accession number: O22604

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	March 6, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program