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Protein

Probable aquaporin TIP3-2

Gene

TIP3-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aquaporin TIP3-2
Alternative name(s):
Beta-tonoplast intrinsic protein
Short name:
Beta-TIP
Tonoplast intrinsic protein 3-2
Short name:
AtTIP3;2
Cleaved into the following chain:
Gene namesi
Name:TIP3-2
Ordered Locus Names:At1g17810
ORF Names:F2H15.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G17810.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626CytoplasmicSequence analysisAdd
BLAST
Transmembranei27 – 4721Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini48 – 6619VacuolarSequence analysisAdd
BLAST
Transmembranei67 – 8721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini88 – 11023CytoplasmicSequence analysisAdd
BLAST
Transmembranei111 – 13121Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini132 – 15120VacuolarSequence analysisAdd
BLAST
Transmembranei152 – 17221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini173 – 1786CytoplasmicSequence analysis
Transmembranei179 – 19921Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini200 – 22627VacuolarSequence analysisAdd
BLAST
Transmembranei227 – 24721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini248 – 26720CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Probable aquaporin TIP3-2PRO_0000064015Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 267266Probable aquaporin TIP3-2, N-terminally processedPRO_0000425763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylalanine; in Probable aquaporin TIP3-2, N-terminally processedBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO22588.
PRIDEiO22588.

Expressioni

Tissue specificityi

Predominantly expressed in developing seeds. Also expressed in rosette leaves.1 Publication

Gene expression databases

GenevisibleiO22588. AT.

Interactioni

Protein-protein interaction databases

BioGridi23598. 4 interactions.
STRINGi3702.AT1G17810.1.

Structurei

3D structure databases

ProteinModelPortaliO22588.
SMRiO22588. Positions 24-248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi93 – 953NPA 1
Motifi207 – 2093NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiO22588.
KOiK09873.
OMAiHHSTHQP.
OrthoDBiEOG09360J95.
PhylomeDBiO22588.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O22588-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATSARRAYG FGRADEATHP DSIRATLAEF LSTFVFVFAG EGSILALDKL
60 70 80 90 100
YWDTAAHTGT NTPGGLVLVA LAHALALFAA VSAAINVSGG HVNPAVTFAA
110 120 130 140 150
LIGGRISVIR AIYYWVAQLI GAILACLLLR LATNGLRPVG FHVASGVSEL
160 170 180 190 200
HGLLMEIILT FALVYVVYST AIDPKRGSIG IIAPLAIGLI VGANILVGGP
210 220 230 240 250
FDGASMNPAR AFGPALVGWR WSNHWIYWVG PFIGGALAAL IYEYMIIPSV
260
NEPPHHSTHQ PLAPEDY
Length:267
Mass (Da):28,183
Last modified:January 1, 1998 - v1
Checksum:i859E0DE51ACF36FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026275 mRNA. Translation: AAB84183.1.
AC034106 Genomic DNA. Translation: AAF97261.1.
CP002684 Genomic DNA. Translation: AEE29639.1.
AK229035 mRNA. Translation: BAF00920.1.
PIRiB86313.
RefSeqiNP_173223.1. NM_101644.3. [O22588-1]
UniGeneiAt.48202.
At.69974.

Genome annotation databases

EnsemblPlantsiAT1G17810.1; AT1G17810.1; AT1G17810. [O22588-1]
GeneIDi838359.
KEGGiath:AT1G17810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026275 mRNA. Translation: AAB84183.1.
AC034106 Genomic DNA. Translation: AAF97261.1.
CP002684 Genomic DNA. Translation: AEE29639.1.
AK229035 mRNA. Translation: BAF00920.1.
PIRiB86313.
RefSeqiNP_173223.1. NM_101644.3. [O22588-1]
UniGeneiAt.48202.
At.69974.

3D structure databases

ProteinModelPortaliO22588.
SMRiO22588. Positions 24-248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23598. 4 interactions.
STRINGi3702.AT1G17810.1.

Proteomic databases

PaxDbiO22588.
PRIDEiO22588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G17810.1; AT1G17810.1; AT1G17810. [O22588-1]
GeneIDi838359.
KEGGiath:AT1G17810.

Organism-specific databases

TAIRiAT1G17810.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiO22588.
KOiK09873.
OMAiHHSTHQP.
OrthoDBiEOG09360J95.
PhylomeDBiO22588.

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiO22588.

Gene expression databases

GenevisibleiO22588. AT.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIP32_ARATH
AccessioniPrimary (citable) accession number: O22588
Secondary accession number(s): Q0WPM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.