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Protein

Probable aquaporin TIP3-2

Gene

TIP3-2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-ATH-432047. Passive transport by Aquaporins.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aquaporin TIP3-2
Alternative name(s):
Beta-tonoplast intrinsic protein
Short name:
Beta-TIP
Tonoplast intrinsic protein 3-2
Short name:
AtTIP3;2
Cleaved into the following chain:
Gene namesi
Name:TIP3-2
Ordered Locus Names:At1g17810
ORF Names:F2H15.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G17810.
TAIRilocus:2030968. AT1G17810.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 26CytoplasmicSequence analysisAdd BLAST25
Transmembranei27 – 47Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini48 – 66VacuolarSequence analysisAdd BLAST19
Transmembranei67 – 87Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini88 – 110CytoplasmicSequence analysisAdd BLAST23
Transmembranei111 – 131Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini132 – 151VacuolarSequence analysisAdd BLAST20
Transmembranei152 – 172Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini173 – 178CytoplasmicSequence analysis6
Transmembranei179 – 199Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini200 – 226VacuolarSequence analysisAdd BLAST27
Transmembranei227 – 247Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini248 – 267CytoplasmicSequence analysisAdd BLAST20

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004257631 – 267Probable aquaporin TIP3-2Add BLAST267
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000640152 – 267Probable aquaporin TIP3-2, N-terminally processedAdd BLAST266

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in Probable aquaporin TIP3-2, N-terminally processedBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO22588.

Expressioni

Tissue specificityi

Predominantly expressed in developing seeds. Also expressed in rosette leaves.1 Publication

Gene expression databases

ExpressionAtlasiO22588. baseline and differential.
GenevisibleiO22588. AT.

Interactioni

Protein-protein interaction databases

BioGridi23598. 4 interactors.
STRINGi3702.AT1G17810.1.

Structurei

3D structure databases

ProteinModelPortaliO22588.
SMRiO22588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 95NPA 13
Motifi207 – 209NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiO22588.
KOiK09873.
OMAiYMIIPSV.
OrthoDBiEOG09360J95.
PhylomeDBiO22588.

Family and domain databases

CDDicd00333. MIP. 1 hit.
Gene3Di1.20.1080.10. 1 hit.
InterProiView protein in InterPro
IPR023271. Aquaporin-like.
IPR034294. Aquaporin_transptr.
IPR000425. MIP.
IPR022357. MIP_CS.
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiView protein in Pfam
PF00230. MIP. 1 hit.
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
TIGRFAMsiTIGR00861. MIP. 1 hit.
PROSITEiView protein in PROSITE
PS00221. MIP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O22588-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATSARRAYG FGRADEATHP DSIRATLAEF LSTFVFVFAG EGSILALDKL
60 70 80 90 100
YWDTAAHTGT NTPGGLVLVA LAHALALFAA VSAAINVSGG HVNPAVTFAA
110 120 130 140 150
LIGGRISVIR AIYYWVAQLI GAILACLLLR LATNGLRPVG FHVASGVSEL
160 170 180 190 200
HGLLMEIILT FALVYVVYST AIDPKRGSIG IIAPLAIGLI VGANILVGGP
210 220 230 240 250
FDGASMNPAR AFGPALVGWR WSNHWIYWVG PFIGGALAAL IYEYMIIPSV
260
NEPPHHSTHQ PLAPEDY
Length:267
Mass (Da):28,183
Last modified:January 1, 1998 - v1
Checksum:i859E0DE51ACF36FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF026275 mRNA. Translation: AAB84183.1.
AC034106 Genomic DNA. Translation: AAF97261.1.
CP002684 Genomic DNA. Translation: AEE29639.1.
AK229035 mRNA. Translation: BAF00920.1.
PIRiB86313.
RefSeqiNP_173223.1. NM_101644.3. [O22588-1]
UniGeneiAt.48202.
At.69974.

Genome annotation databases

EnsemblPlantsiAT1G17810.1; AT1G17810.1; AT1G17810. [O22588-1]
GeneIDi838359.
GrameneiAT1G17810.1; AT1G17810.1; AT1G17810.
KEGGiath:AT1G17810.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTIP32_ARATH
AccessioniPrimary (citable) accession number: O22588
Secondary accession number(s): Q0WPM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2003
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families