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O22585 (AMYB_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-amylase
EC=3.2.1.2
Alternative name(s):
1,4-alpha-D-glucan maltohydrolase
Gene names
Name:BMY1
Synonyms:BA1
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.

Sequence similarities

Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionbeta-amylase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Beta-amylase
PRO_0000153936

Regions

Region382 – 3832Substrate binding By similarity

Sites

Active site1871Proton donor By similarity
Active site3811Proton acceptor By similarity
Binding site541Substrate By similarity
Binding site941Substrate By similarity
Binding site1021Substrate By similarity
Binding site2961Substrate By similarity
Binding site3011Substrate By similarity
Binding site3431Substrate By similarity
Binding site4211Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O22585 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 80F444482AB15E84

FASTA49656,140
        10         20         30         40         50         60 
MATSNKNMLL NYVPVYVMLP LGVINVNNVF EDPDGLKEQL VQLRAAGVDG VMIDVWWGII 

        70         80         90        100        110        120 
EQKGPKEYDW SAYKSLFQLV QKCGLKLQAI MSFHQCGGNV GDVVNIPLPK WVLDIGESDP 

       130        140        150        160        170        180 
DIFYTNRSGI RNQEYLSIGV DNKPIFHGRT AIEIYSDYMK SFRENMSDLL KSEVIIDIEV 

       190        200        210        220        230        240 
GLGPAGELRY PSYPQNQGWQ FPGIGEFQCY DKYLRESFKA AAAKAGHSEW ELPDDAGTYN 

       250        260        270        280        290        300 
DVPESTEFFK TNGTYLTEKG KFFLTWYSNQ LLNHGDQILD EANKAFLGCK VKLAIKVSGI 

       310        320        330        340        350        360 
HWWYKAPNHA AELTAGYYNL DDRDGYRPIA KIVSRHHAIL NFTCLEMRDS EQSSDAHSSP 

       370        380        390        400        410        420 
QKLVQQVLSG GWRENIEVAG ENALSRYDAT AYNQIILNAR PQGVNKDGPP KLRMYGVTYL 

       430        440        450        460        470        480 
RLSDDLMQQS NFDIFKKFVV KMHADQDYCS DPEKYNHGIP PLKRSGPKIP DDVLNEATKP 

       490 
IPPFPWDSET DMKVDG

« Hide

References

[1]"Expression of beta-amylase from alfalfa taproots."
Gana J.A., Kalengamaliro N.E., Cunningham S.M., Volenec J.J.
Plant Physiol. 118:1495-1506(1998) [PubMed: 9847126] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Root.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF026217 mRNA. Translation: AAD04188.1.
PIRT09300.

3D structure databases

ProteinModelPortalO22585.
SMRO22585. Positions 7-496.
ModBaseSearch...

Protein family/group databases

CAZyGH14. Glycoside Hydrolase Family 14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001554. Glyco_hydro_14.
IPR018238. Glyco_hydro_14_CS.
IPR001371. Glyco_hydro_14B_pln.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PfamPF01373. Glyco_hydro_14. 1 hit.
[Graphical view]
PRINTSPR00750. BETAAMYLASE.
PR00842. GLHYDLASE14B.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00506. BETA_AMYLASE_1. 1 hit.
PS00679. BETA_AMYLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYB_MEDSA
AccessionPrimary (citable) accession number: O22585
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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