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Protein

Choline monooxygenase, chloroplastic

Gene

CMO

Organism
Beta vulgaris (Sugar beet)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first step of the osmoprotectant glycine betaine synthesis.

Catalytic activityi

Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine aldehyde from choline (monooxygenase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Choline monooxygenase, chloroplastic (CMO)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine aldehyde from choline (monooxygenase route), the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi169Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi171Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi188Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi191Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi294IronSequence analysis1
Metal bindingi299IronSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00529; UER00430.

Names & Taxonomyi

Protein namesi
Recommended name:
Choline monooxygenase, chloroplastic (EC:1.14.15.7)
Gene namesi
Name:CMO
OrganismiBeta vulgaris (Sugar beet)
Taxonomic identifieri161934 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeBetoideaeBeta

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 65ChloroplastBy similarityAdd BLAST65
ChainiPRO_000002092766 – 446Choline monooxygenase, chloroplasticAdd BLAST381

Proteomic databases

PRIDEiO22553.

Expressioni

Tissue specificityi

Expressed in roots and leaves.

Inductioni

By salt and drought stress.

Structurei

3D structure databases

ProteinModelPortaliO22553.
SMRiO22553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini127 – 234RieskePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the choline monooxygenase family.Curated
Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK00499.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22553-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASATTMLL KYPTLCAMPN SSSSSNNNDL PTSIPLNNNN NLLSNKNKIL
60 70 80 90 100
QTPNINTSTN KIITKAVASP VFPTLKTTSN TPSSIRSLVH EFDPEIPPED
110 120 130 140 150
ALTPPSTWYT EPAFYSHELE RIFYKGWQVA GYSEQVKEKN QYFTGSLGNV
160 170 180 190 200
EYLVSRDGQG ELHAFHNVCT HRASILACGS GKKSCFVCPY HGWVYGLDGS
210 220 230 240 250
LAKASKATET QNLDPKELGL APLKVAEWGP FILISLDRSL DANADVGTEW
260 270 280 290 300
IGKSAEDVKA HAFDPNLKFT HRSEFPMECN WKVFCDNYLD SSYHVPYAHK
310 320 330 340 350
YYAAELDFDT YNTEMIEKCV IQRVGSSSNK PDGFDRLGTE AFYAFIYPNF
360 370 380 390 400
AVERYGTWMT TMHVVPMGQR KCKLVVDYYL EKAMLDDKAY IDKGIAINDN
410 420 430 440
VQKEDKVLCE SVQRGLETPA YRSGRYVMPI EKGIHHFHCW LHETLQ
Length:446
Mass (Da):50,219
Last modified:January 1, 1998 - v1
Checksum:i5D8518485D020402
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023132 mRNA. Translation: AAB80954.1.
PIRiT14542.
RefSeqiXP_010682183.1. XM_010683881.1.

Genome annotation databases

GeneIDi104897072.
KEGGibvg:104897072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023132 mRNA. Translation: AAB80954.1.
PIRiT14542.
RefSeqiXP_010682183.1. XM_010683881.1.

3D structure databases

ProteinModelPortaliO22553.
SMRiO22553.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO22553.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi104897072.
KEGGibvg:104897072.

Phylogenomic databases

KOiK00499.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00430.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.90.380.10. 2 hits.
InterProiIPR017941. Rieske_2Fe-2S.
IPR015879. Ring_hydroxy_dOase_asu_C_dom.
IPR001663. Rng_hydr_dOase-A.
[Graphical view]
PfamiPF00355. Rieske. 1 hit.
PF00848. Ring_hydroxyl_A. 1 hit.
[Graphical view]
PRINTSiPR00090. RNGDIOXGNASE.
SUPFAMiSSF50022. SSF50022. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHMO_BETVU
AccessioniPrimary (citable) accession number: O22553
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.