O22553 (CHMO_BETVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Choline monooxygenase, chloroplastic
EC=1.14.15.7

Gene names

Name:

CMO

Organism

Beta vulgaris (Sugar beet)

Taxonomic identifier

161934 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Beta

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the first step of the osmoprotectant glycine betaine synthesis.
Catalytic activity	Choline + O2 + 2 reduced ferredoxin + 2 H+ = betaine aldehyde hydrate + H2O + 2 oxidized ferredoxin.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 iron ion Probable. Magnesium By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (monooxygenase route): step 1/1.
Subcellular location	Plastid › chloroplast stroma By similarity.
Tissue specificity	Expressed in roots and leaves.
Induction	By salt and drought stress.
Sequence similarities	Belongs to the choline monooxygenase family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW choline monooxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 65	65	Chloroplast By similarity
	Chain	66 – 446	381	Choline monooxygenase, chloroplastic		PRO_0000020927
Regions
	Domain	127 – 234	108	Rieske
Sites
	Metal binding	169	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	171	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	188	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	191	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	294	1	Iron Potential
	Metal binding	299	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	O22553 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5D8518485D020402	FASTA	446	50,219
	10 20 30 40 50 60 MAASATTMLL KYPTLCAMPN SSSSSNNNDL PTSIPLNNNN NLLSNKNKIL QTPNINTSTN 70 80 90 100 110 120 KIITKAVASP VFPTLKTTSN TPSSIRSLVH EFDPEIPPED ALTPPSTWYT EPAFYSHELE 130 140 150 160 170 180 RIFYKGWQVA GYSEQVKEKN QYFTGSLGNV EYLVSRDGQG ELHAFHNVCT HRASILACGS 190 200 210 220 230 240 GKKSCFVCPY HGWVYGLDGS LAKASKATET QNLDPKELGL APLKVAEWGP FILISLDRSL 250 260 270 280 290 300 DANADVGTEW IGKSAEDVKA HAFDPNLKFT HRSEFPMECN WKVFCDNYLD SSYHVPYAHK 310 320 330 340 350 360 YYAAELDFDT YNTEMIEKCV IQRVGSSSNK PDGFDRLGTE AFYAFIYPNF AVERYGTWMT 370 380 390 400 410 420 TMHVVPMGQR KCKLVVDYYL EKAMLDDKAY IDKGIAINDN VQKEDKVLCE SVQRGLETPA 430 440 YRSGRYVMPI EKGIHHFHCW LHETLQ

References

[1]	"Osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth." Russell B.L., Rathinasabapathi B., Hanson A.D. Plant Physiol. 116:859-865(1998) [PubMed: 9489025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Great Western D-2. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023132 mRNA. Translation: AAB80954.1.
PIR	T14542.
3D structure databases
ProteinModelPortal	O22553.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR001663. Rng_hydr_dOase-A. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHMO_BETVU

Accession

Primary (citable) accession number: O22553

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	January 1, 1998
Last modified:	June 28, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program