O22508 (LOX18_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable linoleate 9S-lipoxygenase 8
EC=1.13.11.58

Gene names

Name:	LOX1.8
Synonyms:	PLOX2

Organism

Solanum tuberosum (Potato) [Reference proteome]

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure By similarity.
Catalytic activity	Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	Probable linoleate 9S-lipoxygenase 8		PRO_0000412926
Regions
	Domain	33 – 160	128	PLAT
	Domain	163 – 861	699	Lipoxygenase
Sites
	Metal binding	522	1	Iron; catalytic By similarity
	Metal binding	527	1	Iron; catalytic By similarity
	Metal binding	713	1	Iron; catalytic By similarity
	Metal binding	717	1	Iron; catalytic By similarity
	Metal binding	861	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22508 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 92C31F6EAF55FB58	FASTA	861	97,020
	10 20 30 40 50 60 MIGQITSGLF GGHDDSKKVK GTVVMMNKNV LDFTDLASSL TGKIFDVLGQ KVSFQLISSV 70 80 90 100 110 120 QGDPTNGLQG KHSNPAYLEN SLFTLTPLTA GSETAFGVTF DWNEEFGVPG AFIIKNMHIT 130 140 150 160 170 180 EFFLKSLTLE DVPNHGKVHF VCNSWVYPSL NYKSDRIFFA NQPYLPSETP ELLRKYRENE 190 200 210 220 230 240 LLTLRGDGTG KREAWDRIYD YDIYNDLGNP DQGKENVRTT LGGSAEYPYP RRGRTGRPPT 250 260 270 280 290 300 RTDPKVKSRI PLILSLDIYV PRDERFGHLK MSDFLTYALK SIVQFILPEL HALFDGTPNE 310 320 330 340 350 360 FDSFEDVLRL YEGGIKLPQG PLFKALTAAI PLEMIRELLR TDGEGILRFP TPLVIKDSKT 370 380 390 400 410 420 AWRTDEEFAR EMLAGVNPII ISRLQEFPPK SKLDPEAYGN QNSTITAEHI EDKLDGLTVD 430 440 450 460 470 480 EAMNNNKLFI LNHHDVIIPY LRRINTTITK TYASRTLLFL QDNGSLKPLA IELSLPHPDG 490 500 510 520 530 540 DQFGVTSKVY TPTDQGVESS IWQLAKAYVA VNDTGVHQLI SHWLNTHAVI EPFVIATNRQ 550 560 570 580 590 600 LSVLHPIHKL LYPHFRDTMN INASARQILV NAGGVLESTV FQSKFAMEMS AVVYKDWVFP 610 620 630 640 650 660 DQALPADLVK RGVAVEDSSS PHGVRLLIED YPYAVDGLEI WSAIKSWVTD YCSFYYGSDE 670 680 690 700 710 720 EILKDNELQA WWKELREVGH GDKKNEPWWP EMKTPQELID SCTTIIWIAS ALHAAVNFGQ 730 740 750 760 770 780 YPYAGYLPNR PTVSRRFMPE PGTPEYEELK RNPDKAFLKT ITAQLQTLLG VSLVEILSRH 790 800 810 820 830 840 TTDEIYLGQR ESPEWTKDKE PLAAFDRFGK KLTDIEKQII QRNGDNILTN RSGPVNAPYT 850 860 LLFPTSEGGL TGKGIPNSVS I

References

[1]	"Characterization of potato tuber lipoxygenase cDNAs and lipoxygenase expression in potato tubers and leaves." Fidantsef A.L., Bostock R.M. Physiol. Plantarum 102:257-271(1998) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lemhi Russet. Tissue: Tuber.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019614 mRNA. Translation: AAB81595.1.
3D structure databases
ProteinModelPortal	O22508.
SMR	O22508. Positions 18-861.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00382.
Family and domain databases
Gene3D	2.60.60.20. 1 hit. 4.10.372.10. 1 hit.
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. IPR027433. Lipoxygenase_domain_3. [Graphical view]
PANTHER	PTHR11771. PTHR11771. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
SUPFAM	SSF49723. Lipase_LipOase. 1 hit. SSF48484. Lipoxygenase. 1 hit.
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX18_SOLTU

Accession

Primary (citable) accession number: O22508

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program