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O22506 (GLNA2_DAUCA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase, chloroplastic

EC=6.3.1.2
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene names
Name:GLN2
OrganismDaucus carota (Carrot)
Taxonomic identifier4039 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration By similarity.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Plastidchloroplast By similarity.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 432Glutamine synthetase, chloroplasticPRO_0000011177

Sequences

Sequence LengthMass (Da)Tools
O22506 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 20EC0A4CF8E35345

FASTA43247,764
        10         20         30         40         50         60 
MAQILAPSVQ WQMRFTKNST EVSSMTSKMW GSLFLKQNKK APARSSTKYR ALAVKSEDGT 

        70         80         90        100        110        120 
INRMEDLLNL DVTPYTDKII AEYIWIGGTG IDVRSKSRTI SKPVEHPSEL PKWNYDGSST 

       130        140        150        160        170        180 
GQAPGDDSEV ILYPQAIFKD PFRGGNNILV ICDTYTPQGE PIPTNKRHKA AQIFSDAKVL 

       190        200        210        220        230        240 
GEVPWFGIEQ EYTLMQQDVN WPLGWNVGGY PGPQGPYYCA AGADKSFGRD ISDAHYKACL 

       250        260        270        280        290        300 
YAGINISGTN GEVMPGQWEF QVGPSVGIEA GDHIWCARYL LERITEQAGV VLTLDPKPID 

       310        320        330        340        350        360 
GDWNGAGCHT NYSTKSMREE GGFEVIKKAI LNLSLRHKEH ISAYGEGNER RLTGKHETAS 

       370        380        390        400        410        420 
IDSFSWGVAD RGCSIRVGRD TEKEGKGYLE DRRPASNMDP YVVTGLLAET TLLWEPTLEA 

       430 
EALAAQKLSL NV 

« Hide

References

[1]Higashi K., Kamada H.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. US-Harumakigosun.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019561 mRNA. Translation: AAB71693.1.
PIRT14292.

3D structure databases

ProteinModelPortalO22506.
SMRO22506. Positions 64-415.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO22506.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_DAUCA
AccessionPrimary (citable) accession number: O22506
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families