Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Daucus carota (Wild carrot)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GLN2
OrganismiDaucus carota (Wild carrot)
Taxonomic identifieri4039 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Subcellular locationi

  1. Plastidchloroplast By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 432Glutamine synthetase, chloroplasticPRO_0000011177
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiO22506.

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO22506.
SMRiO22506. Positions 64-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILAPSVQ WQMRFTKNST EVSSMTSKMW GSLFLKQNKK APARSSTKYR
60 70 80 90 100
ALAVKSEDGT INRMEDLLNL DVTPYTDKII AEYIWIGGTG IDVRSKSRTI
110 120 130 140 150
SKPVEHPSEL PKWNYDGSST GQAPGDDSEV ILYPQAIFKD PFRGGNNILV
160 170 180 190 200
ICDTYTPQGE PIPTNKRHKA AQIFSDAKVL GEVPWFGIEQ EYTLMQQDVN
210 220 230 240 250
WPLGWNVGGY PGPQGPYYCA AGADKSFGRD ISDAHYKACL YAGINISGTN
260 270 280 290 300
GEVMPGQWEF QVGPSVGIEA GDHIWCARYL LERITEQAGV VLTLDPKPID
310 320 330 340 350
GDWNGAGCHT NYSTKSMREE GGFEVIKKAI LNLSLRHKEH ISAYGEGNER
360 370 380 390 400
RLTGKHETAS IDSFSWGVAD RGCSIRVGRD TEKEGKGYLE DRRPASNMDP
410 420 430
YVVTGLLAET TLLWEPTLEA EALAAQKLSL NV
Length:432
Mass (Da):47,764
Last modified:January 1, 1998 - v1
Checksum:i20EC0A4CF8E35345
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019561 mRNA. Translation: AAB71693.1.
PIRiT14292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019561 mRNA. Translation: AAB71693.1.
PIRiT14292.

3D structure databases

ProteinModelPortaliO22506.
SMRiO22506. Positions 64-415.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO22506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Higashi K., Kamada H.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. US-Harumakigosun.
    Tissue: Leaf.

Entry informationi

Entry nameiGLNA2_DAUCA
AccessioniPrimary (citable) accession number: O22506
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.