ID   GLNA1_DAUCA             Reviewed;         352 AA.
AC   O22504;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   08-NOV-2023, entry version 87.
DE   RecName: Full=Glutamine synthetase cytosolic isozyme;
DE            EC=6.3.1.2;
DE   AltName: Full=GS1;
DE   AltName: Full=Glutamate--ammonia ligase;
GN   Name=GLN1;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. US-Harumakigosun;
RA   Higashi K., Kamada H.;
RL   Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC         phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC   -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR   EMBL; AF019559; AAB71691.1; -; mRNA.
DR   PIR; T14290; T14290.
DR   AlphaFoldDB; O22504; -.
DR   SMR; O22504; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamine synthetase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.10.20.70; Glutamine synthetase, N-terminal domain; 1.
DR   Gene3D; 3.30.590.10; Glutamine synthetase/guanido kinase, catalytic domain; 2.
DR   InterPro; IPR008147; Gln_synt_N.
DR   InterPro; IPR036651; Gln_synt_N_sf.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR008146; Gln_synth_cat_dom.
DR   InterPro; IPR027303; Gln_synth_gly_rich_site.
DR   InterPro; IPR027302; Gln_synth_N_conserv_site.
DR   PANTHER; PTHR20852; GLUTAMINE SYNTHETASE; 1.
DR   PANTHER; PTHR20852:SF86; GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-3; 1.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   Pfam; PF03951; Gln-synt_N; 1.
DR   SMART; SM01230; Gln-synt_C; 1.
DR   SUPFAM; SSF54368; Glutamine synthetase, N-terminal domain; 1.
DR   SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS00181; GLNA_ATP; 1.
DR   PROSITE; PS51986; GS_BETA_GRASP; 1.
DR   PROSITE; PS51987; GS_CATALYTIC; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN           1..352
FT                   /note="Glutamine synthetase cytosolic isozyme"
FT                   /id="PRO_0000153170"
FT   DOMAIN          19..98
FT                   /note="GS beta-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT   DOMAIN          105..352
FT                   /note="GS catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
SQ   SEQUENCE   352 AA;  38340 MW;  D12BE42E4F56E0A5 CRC64;
     MASLTDLINL DLSDTTDKFI AEYIWIDAVG GLRSKARTLS GPVDDPTKLP KWNFDGSSTG
     QGPGDDSEVI IYPQAIFKDP FRRGNHILVM CDTYTPAGEP IPTNKRCNAA KIFSHPDVAA
     EVPWFGIEQE YTLLKKEVNC PIGCPTGGYP GPQGPYYCGI GADKAFGRDI VDAHYKACLY
     AGINISGING EVMPGQWEFQ VGPAVGISAG DELWVARYIL ERITEIAGVV VSLDPKPIPG
     DWNGAGAHTN YSTKSMRNEG GFEIIKKAIA KLETKHAQHI AAYGEGNERR LTGKHETASI
     HKFSWGVANR GASVRVGRDT EKEGKGYFED RRPASNMEPY VVTSMIAETT IL
//