Skip Header

Contribute Send feedback
Read comments (?) or add your own

O22494 (GSHB_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase, chloroplastic
Short name=GSH synthetase
Short name=GSH-S
Short name=Glutathione synthase
EC=6.3.2.3
Gene names
Name:GSH2
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length546 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast Potential.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6363Chloroplast Potential
Chain64 – 546483Glutathione synthetase, chloroplastic
PRO_0000013060

Regions

Nucleotide binding435 – 44410ATP By similarity
Nucleotide binding471 – 4744ATP By similarity
Region220 – 2234Substrate binding By similarity
Region288 – 2903Substrate binding By similarity
Region342 – 3454Substrate binding By similarity
Region533 – 5342Substrate binding By similarity

Sites

Metal binding2161Magnesium By similarity
Metal binding2181Magnesium By similarity
Metal binding4391Magnesium By similarity
Binding site2001Substrate By similarity
Binding site2161ATP By similarity
Binding site2941Substrate By similarity
Binding site3811ATP By similarity
Binding site4461ATP By similarity
Binding site4971ATP By similarity
Binding site5221Substrate By similarity
Binding site5241ATP By similarity
Binding site5301ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
O22494 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BD1CD811647C43D4

FASTA54661,174
        10         20         30         40         50         60 
MGSGCSSPSI SLTTIATSHF QSQESLSNSL NFYSPTRFLE PHLLKSSKIF IPKSPLKCAK 

        70         80         90        100        110        120 
VPEMQTQLED SAKPIVDPHD IDSKLVQKLA NDALVWCPLR GLLVGDRNSE RSGTIPGVDM 

       130        140        150        160        170        180 
VHAPVALIPM SFPESHWKQA CEVAPIFNEL VDRVSQDGEF LQQSLSRTRK ADPFTSRLLE 

       190        200        210        220        230        240 
IHSKMLEINK LEEIRLGLHR SDYMLDEQTK LLLQIELNTI SSSFPGLSCL VSELHRSLLQ 

       250        260        270        280        290        300 
QYREDIASDP NRIPANNAVN QFAEALAKAW NEYGDPRAVI IFVVQAEERN MYDQHWLSAS 

       310        320        330        340        350        360 
LRERHQVTTI RKTLAEIDAL GELQQDGTLV VDGQAVAVIY FRAGYAPSDY HSESEWKARL 

       370        380        390        400        410        420 
LMEQSRAVKC PSISYHLAGS KKIQQELAKP NVLERFLENK DDIAKLRKCF AGLWSLDESD 

       430        440        450        460        470        480 
IVKDAIERPE LYVMKPQREG GGNNIYGEDV RGALLKLQKE GTGSDAAYIL MQRIFPKISH 

       490        500        510        520        530        540 
SILMREGISH KEETISELGI YGTYLRNKTE VLINQQAGYL MRTKVSSSDE GGVAAGFAVL 


DSIYLV

« Hide

References

[1]Kovari I.A., Goldsbrough P.B.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Ohio state 4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF017984 mRNA. Translation: AAB71231.1.
PIRT04336.
RefSeqNP_001234014.1. NM_001247085.1.
UniGeneLes.3449.

3D structure databases

ProteinModelPortalO22494.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID543537.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSHB_SOLLC
AccessionPrimary (citable) accession number: O22494
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: March 6, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents