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Protein

Glutathione synthetase, chloroplastic

Gene

GSH2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathway:iglutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase, chloroplastic (GSH1)
  2. Glutathione synthetase, chloroplastic (GSH2), Glutathione synthetase (GSH2)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei200 – 2001SubstrateBy similarity
Metal bindingi216 – 2161MagnesiumBy similarity
Binding sitei216 – 2161ATPBy similarity
Metal bindingi218 – 2181MagnesiumBy similarity
Binding sitei294 – 2941SubstrateBy similarity
Binding sitei381 – 3811ATPBy similarity
Metal bindingi439 – 4391MagnesiumBy similarity
Binding sitei446 – 4461ATPBy similarity
Binding sitei497 – 4971ATPBy similarity
Binding sitei522 – 5221SubstrateBy similarity
Binding sitei524 – 5241ATPBy similarity
Binding sitei530 – 5301ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi435 – 44410ATPBy similarity
Nucleotide bindingi471 – 4744ATPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase, chloroplastic (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Short name:
Glutathione synthase
Gene namesi
Name:GSH2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
BLAST
Chaini64 – 546483Glutathione synthetase, chloroplasticPRO_0000013060Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi4081.Solyc01g098610.2.1.

Structurei

3D structure databases

ProteinModelPortaliO22494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni220 – 2234Substrate bindingBy similarity
Regioni288 – 2903Substrate bindingBy similarity
Regioni342 – 3454Substrate bindingBy similarity
Regioni533 – 5342Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiO22494.
KOiK01920.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22494-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSGCSSPSI SLTTIATSHF QSQESLSNSL NFYSPTRFLE PHLLKSSKIF
60 70 80 90 100
IPKSPLKCAK VPEMQTQLED SAKPIVDPHD IDSKLVQKLA NDALVWCPLR
110 120 130 140 150
GLLVGDRNSE RSGTIPGVDM VHAPVALIPM SFPESHWKQA CEVAPIFNEL
160 170 180 190 200
VDRVSQDGEF LQQSLSRTRK ADPFTSRLLE IHSKMLEINK LEEIRLGLHR
210 220 230 240 250
SDYMLDEQTK LLLQIELNTI SSSFPGLSCL VSELHRSLLQ QYREDIASDP
260 270 280 290 300
NRIPANNAVN QFAEALAKAW NEYGDPRAVI IFVVQAEERN MYDQHWLSAS
310 320 330 340 350
LRERHQVTTI RKTLAEIDAL GELQQDGTLV VDGQAVAVIY FRAGYAPSDY
360 370 380 390 400
HSESEWKARL LMEQSRAVKC PSISYHLAGS KKIQQELAKP NVLERFLENK
410 420 430 440 450
DDIAKLRKCF AGLWSLDESD IVKDAIERPE LYVMKPQREG GGNNIYGEDV
460 470 480 490 500
RGALLKLQKE GTGSDAAYIL MQRIFPKISH SILMREGISH KEETISELGI
510 520 530 540
YGTYLRNKTE VLINQQAGYL MRTKVSSSDE GGVAAGFAVL DSIYLV
Length:546
Mass (Da):61,174
Last modified:January 1, 1998 - v1
Checksum:iBD1CD811647C43D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017984 mRNA. Translation: AAB71231.1.
PIRiT04336.
RefSeqiNP_001234014.1. NM_001247085.2.
UniGeneiLes.3449.

Genome annotation databases

GeneIDi543537.
KEGGisly:543537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017984 mRNA. Translation: AAB71231.1.
PIRiT04336.
RefSeqiNP_001234014.1. NM_001247085.2.
UniGeneiLes.3449.

3D structure databases

ProteinModelPortaliO22494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc01g098610.2.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi543537.
KEGGisly:543537.

Phylogenomic databases

InParanoidiO22494.
KOiK01920.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Kovari I.A., Goldsbrough P.B.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ohio state 4.

Entry informationi

Entry nameiGSHB_SOLLC
AccessioniPrimary (citable) accession number: O22494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.