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O22494

- GSHB_SOLLC

UniProt

O22494 - GSHB_SOLLC

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Protein

Glutathione synthetase, chloroplastic

Gene
GSH2
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei200 – 2001Substrate By similarity
Metal bindingi216 – 2161Magnesium By similarity
Binding sitei216 – 2161ATP By similarity
Metal bindingi218 – 2181Magnesium By similarity
Binding sitei294 – 2941Substrate By similarity
Binding sitei381 – 3811ATP By similarity
Metal bindingi439 – 4391Magnesium By similarity
Binding sitei446 – 4461ATP By similarity
Binding sitei497 – 4971ATP By similarity
Binding sitei522 – 5221Substrate By similarity
Binding sitei524 – 5241ATP By similarity
Binding sitei530 – 5301ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi435 – 44410ATP By similarity
Nucleotide bindingi471 – 4744ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: UniProtKB-EC
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. response to jasmonic acid Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase, chloroplastic (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Short name:
Glutathione synthase
Gene namesi
Name:GSH2
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

Plastidchloroplast Reviewed prediction

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
  2. cytosol Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6363Chloroplast Reviewed predictionAdd
BLAST
Chaini64 – 546483Glutathione synthetase, chloroplasticPRO_0000013060Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO22494.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni220 – 2234Substrate binding By similarity
Regioni288 – 2903Substrate binding By similarity
Regioni342 – 3454Substrate binding By similarity
Regioni533 – 5342Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK01920.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22494-1 [UniParc]FASTAAdd to Basket

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MGSGCSSPSI SLTTIATSHF QSQESLSNSL NFYSPTRFLE PHLLKSSKIF    50
IPKSPLKCAK VPEMQTQLED SAKPIVDPHD IDSKLVQKLA NDALVWCPLR 100
GLLVGDRNSE RSGTIPGVDM VHAPVALIPM SFPESHWKQA CEVAPIFNEL 150
VDRVSQDGEF LQQSLSRTRK ADPFTSRLLE IHSKMLEINK LEEIRLGLHR 200
SDYMLDEQTK LLLQIELNTI SSSFPGLSCL VSELHRSLLQ QYREDIASDP 250
NRIPANNAVN QFAEALAKAW NEYGDPRAVI IFVVQAEERN MYDQHWLSAS 300
LRERHQVTTI RKTLAEIDAL GELQQDGTLV VDGQAVAVIY FRAGYAPSDY 350
HSESEWKARL LMEQSRAVKC PSISYHLAGS KKIQQELAKP NVLERFLENK 400
DDIAKLRKCF AGLWSLDESD IVKDAIERPE LYVMKPQREG GGNNIYGEDV 450
RGALLKLQKE GTGSDAAYIL MQRIFPKISH SILMREGISH KEETISELGI 500
YGTYLRNKTE VLINQQAGYL MRTKVSSSDE GGVAAGFAVL DSIYLV 546
Length:546
Mass (Da):61,174
Last modified:January 1, 1998 - v1
Checksum:iBD1CD811647C43D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017984 mRNA. Translation: AAB71231.1.
PIRiT04336.
RefSeqiNP_001234014.1. NM_001247085.2.
UniGeneiLes.3449.

Genome annotation databases

GeneIDi543537.
KEGGisly:543537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017984 mRNA. Translation: AAB71231.1 .
PIRi T04336.
RefSeqi NP_001234014.1. NM_001247085.2.
UniGenei Les.3449.

3D structure databases

ProteinModelPortali O22494.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 543537.
KEGGi sly:543537.

Phylogenomic databases

KOi K01920.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00210 .

Family and domain databases

Gene3Di 1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProi IPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR11130. PTHR11130. 1 hit.
Pfami PF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001558. GSHase. 1 hit.
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Kovari I.A., Goldsbrough P.B.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ohio state 4.

Entry informationi

Entry nameiGSHB_SOLLC
AccessioniPrimary (citable) accession number: O22494
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: January 1, 1998
Last modified: March 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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