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O22494

- GSHB_SOLLC

UniProt

O22494 - GSHB_SOLLC

Protein

Glutathione synthetase, chloroplastic

Gene

GSH2

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei200 – 2001SubstrateBy similarity
    Metal bindingi216 – 2161MagnesiumBy similarity
    Binding sitei216 – 2161ATPBy similarity
    Metal bindingi218 – 2181MagnesiumBy similarity
    Binding sitei294 – 2941SubstrateBy similarity
    Binding sitei381 – 3811ATPBy similarity
    Metal bindingi439 – 4391MagnesiumBy similarity
    Binding sitei446 – 4461ATPBy similarity
    Binding sitei497 – 4971ATPBy similarity
    Binding sitei522 – 5221SubstrateBy similarity
    Binding sitei524 – 5241ATPBy similarity
    Binding sitei530 – 5301ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi435 – 44410ATPBy similarity
    Nucleotide bindingi471 – 4744ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. glutathione binding Source: UniProtKB
    3. glutathione synthase activity Source: UniProtKB-EC
    4. magnesium ion binding Source: UniProtKB
    5. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. response to jasmonic acid Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase, chloroplastic (EC:6.3.2.3)
    Short name:
    GSH synthetase
    Short name:
    GSH-S
    Short name:
    Glutathione synthase
    Gene namesi
    Name:GSH2
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Unplaced

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell
    2. cytosol Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6363ChloroplastSequence AnalysisAdd
    BLAST
    Chaini64 – 546483Glutathione synthetase, chloroplasticPRO_0000013060Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO22494.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni220 – 2234Substrate bindingBy similarity
    Regioni288 – 2903Substrate bindingBy similarity
    Regioni342 – 3454Substrate bindingBy similarity
    Regioni533 – 5342Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the eukaryotic GSH synthase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK01920.

    Family and domain databases

    Gene3Di1.10.1080.10. 1 hit.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProiIPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR11130. PTHR11130. 1 hit.
    PfamiPF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001558. GSHase. 1 hit.
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O22494-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSGCSSPSI SLTTIATSHF QSQESLSNSL NFYSPTRFLE PHLLKSSKIF    50
    IPKSPLKCAK VPEMQTQLED SAKPIVDPHD IDSKLVQKLA NDALVWCPLR 100
    GLLVGDRNSE RSGTIPGVDM VHAPVALIPM SFPESHWKQA CEVAPIFNEL 150
    VDRVSQDGEF LQQSLSRTRK ADPFTSRLLE IHSKMLEINK LEEIRLGLHR 200
    SDYMLDEQTK LLLQIELNTI SSSFPGLSCL VSELHRSLLQ QYREDIASDP 250
    NRIPANNAVN QFAEALAKAW NEYGDPRAVI IFVVQAEERN MYDQHWLSAS 300
    LRERHQVTTI RKTLAEIDAL GELQQDGTLV VDGQAVAVIY FRAGYAPSDY 350
    HSESEWKARL LMEQSRAVKC PSISYHLAGS KKIQQELAKP NVLERFLENK 400
    DDIAKLRKCF AGLWSLDESD IVKDAIERPE LYVMKPQREG GGNNIYGEDV 450
    RGALLKLQKE GTGSDAAYIL MQRIFPKISH SILMREGISH KEETISELGI 500
    YGTYLRNKTE VLINQQAGYL MRTKVSSSDE GGVAAGFAVL DSIYLV 546
    Length:546
    Mass (Da):61,174
    Last modified:January 1, 1998 - v1
    Checksum:iBD1CD811647C43D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017984 mRNA. Translation: AAB71231.1.
    PIRiT04336.
    RefSeqiNP_001234014.1. NM_001247085.2.
    UniGeneiLes.3449.

    Genome annotation databases

    GeneIDi543537.
    KEGGisly:543537.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017984 mRNA. Translation: AAB71231.1 .
    PIRi T04336.
    RefSeqi NP_001234014.1. NM_001247085.2.
    UniGenei Les.3449.

    3D structure databases

    ProteinModelPortali O22494.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 543537.
    KEGGi sly:543537.

    Phylogenomic databases

    KOi K01920.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 1.10.1080.10. 1 hit.
    3.30.1490.50. 1 hit.
    3.30.1490.80. 1 hit.
    3.40.50.1760. 1 hit.
    InterProi IPR004887. Glutathione_synth_subst-bd_euk.
    IPR014042. Glutathione_synthase_a-hlx_euk.
    IPR014709. Glutathione_synthase_dom.
    IPR005615. Glutathione_synthase_euk.
    IPR014049. Glutathione_synthase_N_euk.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR11130. PTHR11130. 1 hit.
    Pfami PF03917. GSH_synth_ATP. 1 hit.
    PF03199. GSH_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001558. GSHase. 1 hit.
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01986. glut_syn_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Kovari I.A., Goldsbrough P.B.
      Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Ohio state 4.

    Entry informationi

    Entry nameiGSHB_SOLLC
    AccessioniPrimary (citable) accession number: O22494
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3