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Protein

WD-40 repeat-containing protein MSI3

Gene

MSI3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
WD-40 repeat-containing protein MSI3
Gene namesi
Name:MSI3
Ordered Locus Names:At4g35050
ORF Names:M4E13.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G35050.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: TAIR
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510821 – 424WD-40 repeat-containing protein MSI3Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO22469.

Expressioni

Gene expression databases

ExpressionAtlasiO22469. baseline and differential.
GenevisibleiO22469. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DDB1AQ9M0V32EBI-1632794,EBI-1632780

Protein-protein interaction databases

BioGridi14939. 2 interactors.
IntActiO22469. 1 interactor.
STRINGi3702.AT4G35050.1.

Structurei

3D structure databases

ProteinModelPortaliO22469.
SMRiO22469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati167 – 207WD 1Add BLAST41
Repeati216 – 256WD 2Add BLAST41
Repeati259 – 299WD 3Add BLAST41
Repeati303 – 343WD 4Add BLAST41
Repeati362 – 402WD 5Add BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi233 – 249DWD boxAdd BLAST17

Domaini

The DWD box is required for interaction with DDB1A.By similarity

Sequence similaritiesi

Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0264. Eukaryota.
ENOG410XNU9. LUCA.
HOGENOMiHOG000160330.
InParanoidiO22469.
KOiK10752.
OMAiMKNENIF.
OrthoDBiEOG09360A5S.
PhylomeDBiO22469.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR022052. Histone-bd_RBBP4_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12265. CAF1C_H4-bd. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEEGKDEA GLDQVEEEFS IWKRNTPFLY DLMISHPLEW PSLTLHWVPS
60 70 80 90 100
TPIPYSKDPY FAVHKLILGT HTSGGAQDFL MVADVVIPTP DAEPGLGGRD
110 120 130 140 150
QEPIVPKVEI KQKIRVDGEV NRARCMPQKP TLVGAKTSGS EVFLFDYARL
160 170 180 190 200
SGKPQTSECD PDLRLMGHEQ EGYGLAWSSF KEGYLLSGSQ DQRICLWDVS
210 220 230 240 250
ATATDKVLNP MHVYEGHQSI IEDVAWHMKN ENIFGSAGDD CQLVIWDLRT
260 270 280 290 300
NQMQHQVKVH EREINYLSFN PFNEWVLATA SSDSTVALFD LRKLTAPLHV
310 320 330 340 350
LSKHEGEVFQ VEWDPNHETV LASSGEDRRL MVWDINRVGD EQLEIELDAE
360 370 380 390 400
DGPPELLFSH GGHKAKISDF AWNKDEPWVI SSVAEDNSLQ VWQMAESIYR
410 420
EDDEDEDDDD EGNQNAQHSN ENQK
Length:424
Mass (Da):47,983
Last modified:January 11, 2001 - v2
Checksum:iD83E2B4913468A0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223 – 224DV → EL in AAB70244 (PubMed:9338962).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016848 mRNA. Translation: AAB70244.1.
AL022023 Genomic DNA. Translation: CAA17770.1.
AL161586 Genomic DNA. Translation: CAB80222.1.
CP002687 Genomic DNA. Translation: AEE86455.1.
PIRiT05775.
RefSeqiNP_195231.1. NM_119671.3.
UniGeneiAt.2099.

Genome annotation databases

EnsemblPlantsiAT4G35050.1; AT4G35050.1; AT4G35050.
GeneIDi829657.
GrameneiAT4G35050.1; AT4G35050.1; AT4G35050.
KEGGiath:AT4G35050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016848 mRNA. Translation: AAB70244.1.
AL022023 Genomic DNA. Translation: CAA17770.1.
AL161586 Genomic DNA. Translation: CAB80222.1.
CP002687 Genomic DNA. Translation: AEE86455.1.
PIRiT05775.
RefSeqiNP_195231.1. NM_119671.3.
UniGeneiAt.2099.

3D structure databases

ProteinModelPortaliO22469.
SMRiO22469.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14939. 2 interactors.
IntActiO22469. 1 interactor.
STRINGi3702.AT4G35050.1.

Proteomic databases

PaxDbiO22469.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G35050.1; AT4G35050.1; AT4G35050.
GeneIDi829657.
GrameneiAT4G35050.1; AT4G35050.1; AT4G35050.
KEGGiath:AT4G35050.

Organism-specific databases

TAIRiAT4G35050.

Phylogenomic databases

eggNOGiKOG0264. Eukaryota.
ENOG410XNU9. LUCA.
HOGENOMiHOG000160330.
InParanoidiO22469.
KOiK10752.
OMAiMKNENIF.
OrthoDBiEOG09360A5S.
PhylomeDBiO22469.

Miscellaneous databases

PROiO22469.

Gene expression databases

ExpressionAtlasiO22469. baseline and differential.
GenevisibleiO22469. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR022052. Histone-bd_RBBP4_N.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12265. CAF1C_H4-bd. 1 hit.
PF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSI3_ARATH
AccessioniPrimary (citable) accession number: O22469
Secondary accession number(s): O49612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.