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O22456

- SEP3_ARATH

UniProt

O22456 - SEP3_ARATH

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Protein
Developmental protein SEPALLATA 3
Gene
SEP3, AGL9, At1g24260, F3I6.19
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development.

GO - Molecular functioni

  1. DNA binding Source: TAIR
  2. protein binding Source: UniProtKB
  3. sequence-specific DNA binding transcription factor activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. cell fate specification Source: TAIR
  2. flower development Source: TAIR
  3. ovule development Source: TAIR
  4. specification of floral organ identity Source: TAIR
  5. specification of floral organ number Source: TAIR
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 3
Alternative name(s):
Agamous-like MADS-box protein AGL9
Gene namesi
Name:SEP3
Synonyms:AGL9
Ordered Locus Names:At1g24260
ORF Names:F3I6.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G24260.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Developmental protein SEPALLATA 3
PRO_0000199485Add
BLAST

Proteomic databases

PaxDbiO22456.
PRIDEiO22456.

Expressioni

Developmental stagei

Expressed early during flower development within petals, stamens, and carpels.

Gene expression databases

GenevestigatoriO22456.

Interactioni

Subunit structurei

Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP178394EBI-592020,EBI-592365
AGL1P293815EBI-592020,EBI-592304
AGL11Q388363EBI-592020,EBI-592343
AP1P356314EBI-592020,EBI-592003
PIP480073EBI-592020,EBI-1395334
SEUQ8W2342EBI-592020,EBI-1771131
SHP2Q5XXE73EBI-592020,EBI-592328
TT16Q8RYD97EBI-592020,EBI-621993

Protein-protein interaction databases

BioGridi24278. 24 interactions.
DIPiDIP-33800N.
IntActiO22456. 19 interactions.
STRINGi3702.AT1G24260.2-P.

Structurei

3D structure databases

ProteinModelPortaliO22456.
SMRiO22456. Positions 2-70.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-box
Add
BLAST
Domaini91 – 18191K-box
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili94 – 17784 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 K-box domain.
Contains 1 MADS-box domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5068.
HOGENOMiHOG000155301.
InParanoidiO22456.
OMAiPLECEPI.
PhylomeDBiO22456.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN    50
RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK 100
LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM 150
LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH 200
QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS 250
I 251
Length:251
Mass (Da):29,066
Last modified:January 1, 1998 - v1
Checksum:i0057CABD3F1AFC40
GO
Isoform 2 (identifier: O22456-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-90: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:250
Mass (Da):28,967
Checksum:iF1AA1EA27D82B63F
GO

Sequence cautioni

The sequence BAC43290.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei90 – 901Missing in isoform 2.
VSP_008893

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRiT00656.
RefSeqiNP_564214.2. NM_102272.3. [O22456-1]
NP_850953.1. NM_180622.2. [O22456-2]
UniGeneiAt.24086.

Genome annotation databases

EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneIDi839040.
KEGGiath:AT1G24260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF015552 mRNA. Translation: AAB67832.1 .
AC002396 Genomic DNA. Translation: AAC00586.1 .
CP002684 Genomic DNA. Translation: AEE30502.1 .
CP002684 Genomic DNA. Translation: AEE30503.1 .
AY088272 mRNA. Translation: AAM65812.1 .
AK118696 mRNA. Translation: BAC43290.1 . Different initiation.
PIRi T00656.
RefSeqi NP_564214.2. NM_102272.3. [O22456-1 ]
NP_850953.1. NM_180622.2. [O22456-2 ]
UniGenei At.24086.

3D structure databases

ProteinModelPortali O22456.
SMRi O22456. Positions 2-70.
ModBasei Search...

Protein-protein interaction databases

BioGridi 24278. 24 interactions.
DIPi DIP-33800N.
IntActi O22456. 19 interactions.
STRINGi 3702.AT1G24260.2-P.

Proteomic databases

PaxDbi O22456.
PRIDEi O22456.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G24260.2 ; AT1G24260.2 ; AT1G24260 . [O22456-1 ]
GeneIDi 839040.
KEGGi ath:AT1G24260.

Organism-specific databases

GeneFarmi 3497. 352.
TAIRi AT1G24260.

Phylogenomic databases

eggNOGi COG5068.
HOGENOMi HOG000155301.
InParanoidi O22456.
OMAi PLECEPI.
PhylomeDBi O22456.

Gene expression databases

Genevestigatori O22456.

Family and domain databases

InterProi IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view ]
Pfami PF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view ]
PRINTSi PR00404. MADSDOMAIN.
SMARTi SM00432. MADS. 1 hit.
[Graphical view ]
SUPFAMi SSF55455. SSF55455. 1 hit.
PROSITEi PS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia."
    Mandel M.A., Yanofsky M.F.
    Sex. Plant Reprod. 11:22-28(1998)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-251.
    Strain: cv. Columbia.
  6. "Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
    Fan H.-Y., Hu Y., Tudor M., Ma H.
    Plant J. 12:999-1010(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "B and C floral organ identity functions require SEPALLATA MADS-box genes."
    Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.
    Nature 405:200-203(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE.
  8. "Complexes of MADS-box proteins are sufficient to convert leaves into floral organs."
    Honma T., Goto K.
    Nature 409:525-529(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. Cited for: INTERACTION WITH AGL16.

Entry informationi

Entry nameiSEP3_ARATH
AccessioniPrimary (citable) accession number: O22456
Secondary accession number(s): Q8GWQ4, Q8L9R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi