Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Developmental protein SEPALLATA 3

Gene

SEP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development.

GO - Molecular functioni

  1. DNA binding Source: TAIR
  2. sequence-specific DNA binding transcription factor activity Source: TAIR

GO - Biological processi

  1. cell fate specification Source: TAIR
  2. flower development Source: TAIR
  3. ovule development Source: TAIR
  4. specification of floral organ identity Source: TAIR
  5. specification of floral organ number Source: TAIR
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_273308. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 3
Alternative name(s):
Agamous-like MADS-box protein AGL9
Gene namesi
Name:SEP3
Synonyms:AGL9
Ordered Locus Names:At1g24260
ORF Names:F3I6.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G24260.

Subcellular locationi

Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Developmental protein SEPALLATA 3PRO_0000199485Add
BLAST

Proteomic databases

PaxDbiO22456.
PRIDEiO22456.

Expressioni

Developmental stagei

Expressed early during flower development within petals, stamens, and carpels.

Gene expression databases

ExpressionAtlasiO22456. baseline and differential.
GenevestigatoriO22456.

Interactioni

Subunit structurei

Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP178394EBI-592020,EBI-592365
AGL1P293815EBI-592020,EBI-592304
AGL11Q388363EBI-592020,EBI-592343
AP1P356314EBI-592020,EBI-592003
PIP480073EBI-592020,EBI-1395334
SEUQ8W2342EBI-592020,EBI-1771131
SHP2Q5XXE73EBI-592020,EBI-592328
TT16Q8RYD97EBI-592020,EBI-621993

Protein-protein interaction databases

BioGridi24278. 24 interactions.
DIPiDIP-33800N.
IntActiO22456. 19 interactions.
STRINGi3702.AT1G24260.2-P.

Structurei

Secondary structure

1
251
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi85 – 11430Combined sources
Turni115 – 1173Combined sources
Helixi125 – 16844Combined sources
Helixi169 – 1724Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OX0X-ray2.49A/B/C/D75-178[»]
SMRiO22456. Positions 2-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini91 – 18191K-boxPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili94 – 17784Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5068.
HOGENOMiHOG000155301.
InParanoidiO22456.
OMAiERQSPHA.
PhylomeDBiO22456.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK
110 120 130 140 150
LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM
160 170 180 190 200
LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH
210 220 230 240 250
QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS

I
Length:251
Mass (Da):29,066
Last modified:December 31, 1997 - v1
Checksum:i0057CABD3F1AFC40
GO
Isoform 2 (identifier: O22456-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-90: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Show »
Length:250
Mass (Da):28,967
Checksum:iF1AA1EA27D82B63F
GO

Sequence cautioni

The sequence BAC43290.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei90 – 901Missing in isoform 2. 1 PublicationVSP_008893

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRiT00656.
RefSeqiNP_564214.2. NM_102272.3. [O22456-1]
NP_850953.1. NM_180622.2. [O22456-2]
UniGeneiAt.24086.

Genome annotation databases

EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneIDi839040.
KEGGiath:AT1G24260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRiT00656.
RefSeqiNP_564214.2. NM_102272.3. [O22456-1]
NP_850953.1. NM_180622.2. [O22456-2]
UniGeneiAt.24086.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OX0X-ray2.49A/B/C/D75-178[»]
SMRiO22456. Positions 2-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24278. 24 interactions.
DIPiDIP-33800N.
IntActiO22456. 19 interactions.
STRINGi3702.AT1G24260.2-P.

Proteomic databases

PaxDbiO22456.
PRIDEiO22456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneIDi839040.
KEGGiath:AT1G24260.

Organism-specific databases

GeneFarmi3497. 352.
TAIRiAT1G24260.

Phylogenomic databases

eggNOGiCOG5068.
HOGENOMiHOG000155301.
InParanoidiO22456.
OMAiERQSPHA.
PhylomeDBiO22456.

Enzyme and pathway databases

ReactomeiREACT_273308. ERK/MAPK targets.

Gene expression databases

ExpressionAtlasiO22456. baseline and differential.
GenevestigatoriO22456.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia."
    Mandel M.A., Yanofsky M.F.
    Sex. Plant Reprod. 11:22-28(1997)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-251.
    Strain: cv. Columbia.
  6. "Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
    Fan H.-Y., Hu Y., Tudor M., Ma H.
    Plant J. 12:999-1010(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "B and C floral organ identity functions require SEPALLATA MADS-box genes."
    Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.
    Nature 405:200-203(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE.
  8. "Complexes of MADS-box proteins are sufficient to convert leaves into floral organs."
    Honma T., Goto K.
    Nature 409:525-529(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. Cited for: INTERACTION WITH AGL16.

Entry informationi

Entry nameiSEP3_ARATH
AccessioniPrimary (citable) accession number: O22456
Secondary accession number(s): Q8GWQ4, Q8L9R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: December 31, 1997
Last modified: March 31, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.