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O22456 (SEP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Developmental protein SEPALLATA 3
Alternative name(s):
Agamous-like MADS-box protein AGL9
Gene names
Name:SEP3
Synonyms:AGL9
Ordered Locus Names:At1g24260
ORF Names:F3I6.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development.

Subunit structure

Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16. Ref.9

Subcellular location

Nucleus By similarity.

Developmental stage

Expressed early during flower development within petals, stamens, and carpels.

Disruption phenotype

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs. Ref.7

Sequence similarities

Contains 1 K-box domain.

Contains 1 MADS-box domain.

Sequence caution

The sequence BAC43290.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O22456-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O22456-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-90: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Developmental protein SEPALLATA 3
PRO_0000199485

Regions

Domain3 – 5755MADS-box
Domain91 – 18191K-box
Coiled coil94 – 17784 Potential

Natural variations

Alternative sequence901Missing in isoform 2.
VSP_008893

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 0057CABD3F1AFC40

FASTA25129,066
        10         20         30         40         50         60 
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN RGKLYEFCSS 

        70         80         90        100        110        120 
SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK LKERYDALQR TQRNLLGEDL 

       130        140        150        160        170        180 
GPLSTKELES LERQLDSSLK QIRALRTQFM LDQLNDLQSK ERMLTETNKT LRLRLADGYQ 

       190        200        210        220        230        240 
MPLQLNPNQE EVDHYGRHHH QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM 

       250 
LGWLPYDTNS I 

« Hide

Isoform 2 [UniParc].

Checksum: F1AA1EA27D82B63F
Show »

FASTA25028,967

References

« Hide 'large scale' references
[1]"The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia."
Mandel M.A., Yanofsky M.F.
Sex. Plant Reprod. 11:22-28(1998) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-251.
Strain: cv. Columbia.
[6]"Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
Fan H.-Y., Hu Y., Tudor M., Ma H.
Plant J. 12:999-1010(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"B and C floral organ identity functions require SEPALLATA MADS-box genes."
Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.
Nature 405:200-203(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE.
[8]"Complexes of MADS-box proteins are sufficient to convert leaves into floral organs."
Honma T., Goto K.
Nature 409:525-529(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[9]"Comprehensive interaction map of the Arabidopsis MADS Box transcription factors."
de Folter S., Immink R.G.H., Kieffer M., Parenicova L., Henz S.R., Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B., Angenent G.C.
Plant Cell 17:1424-1433(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AGL16.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRT00656.
RefSeqNP_564214.2. NM_102272.3.
NP_850953.1. NM_180622.2.
UniGeneAt.24086.

3D structure databases

ProteinModelPortalO22456.
SMRO22456. Positions 2-70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24278. 24 interactions.
DIPDIP-33800N.
IntActO22456. 19 interactions.
STRING3702.AT1G24260.2-P.

Proteomic databases

PaxDbO22456.
PRIDEO22456.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneID839040.
KEGGath:AT1G24260.

Organism-specific databases

GeneFarm3497. 352.
TAIRAT1G24260.

Phylogenomic databases

eggNOGCOG5068.
HOGENOMHOG000155301.
InParanoidO22456.
OMAPLECEPI.
PhylomeDBO22456.
ProtClustDBCLSN2690422.

Gene expression databases

GenevestigatorO22456.

Family and domain databases

InterProIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSPR00404. MADSDOMAIN.
SMARTSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMSSF55455. SSF55455. 1 hit.
PROSITEPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSEP3_ARATH
AccessionPrimary (citable) accession number: O22456
Secondary accession number(s): Q8GWQ4, Q8L9R5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names