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Protein

Developmental protein SEPALLATA 3

Gene

SEP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development (PubMed:10821278, PubMed:11206550). Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3' (PubMed:16080001).3 Publications

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • protein dimerization activity Source: InterPro
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • cell fate specification Source: TAIR
  • flower development Source: TAIR
  • plant ovule development Source: TAIR
  • positive regulation of transcription by RNA polymerase II Source: InterPro
  • specification of floral organ identity Source: TAIR
  • specification of floral organ number Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processDifferentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 3
Alternative name(s):
Agamous-like MADS-box protein AGL9
Gene namesi
Name:SEP3
Synonyms:AGL9
Ordered Locus Names:At1g24260
ORF Names:F3I6.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G24260
TAIRilocus:2032372 AT1G24260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994851 – 251Developmental protein SEPALLATA 3Add BLAST251

Proteomic databases

PaxDbiO22456
PRIDEiO22456

Expressioni

Developmental stagei

Expressed early during flower development within petals, stamens, and carpels.

Gene expression databases

ExpressionAtlasiO22456 baseline and differential
GenevisibleiO22456 AT

Interactioni

Subunit structurei

Forms homodimers (PubMed:25228343). Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16 (PubMed:15805477). Interacts with TT16/AGL32 (PubMed:16080001).3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi24278, 25 interactors
DIPiDIP-33800N
IntActiO22456, 22 interactors
STRINGi3702.AT1G24260.2

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi85 – 114Combined sources30
Turni115 – 117Combined sources3
Helixi125 – 168Combined sources44
Helixi169 – 172Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OX0X-ray2.49A/B/C/D75-178[»]
ProteinModelPortaliO22456
SMRiO22456
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini91 – 181K-boxPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili94 – 177Sequence analysisAdd BLAST84

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014 Eukaryota
COG5068 LUCA
HOGENOMiHOG000155301
InParanoidiO22456
OMAiGYQGQQD
OrthoDBiEOG09360JC8
PhylomeDBiO22456

Family and domain databases

CDDicd00265 MADS_MEF2_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033896 MADS_MEF2-like
IPR002487 TF_Kbox
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF01486 K-box, 1 hit
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS51297 K_BOX, 1 hit
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK
110 120 130 140 150
LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM
160 170 180 190 200
LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH
210 220 230 240 250
QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS

I
Length:251
Mass (Da):29,066
Last modified:January 1, 1998 - v1
Checksum:i0057CABD3F1AFC40
GO
Isoform 2 (identifier: O22456-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-90: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:250
Mass (Da):28,967
Checksum:iF1AA1EA27D82B63F
GO

Sequence cautioni

The sequence BAC43290 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00889390Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015552 mRNA Translation: AAB67832.1
AC002396 Genomic DNA Translation: AAC00586.1
CP002684 Genomic DNA Translation: AEE30502.1
CP002684 Genomic DNA Translation: AEE30503.1
AY088272 mRNA Translation: AAM65812.1
AK118696 mRNA Translation: BAC43290.1 Different initiation.
PIRiT00656
RefSeqiNP_564214.2, NM_102272.4 [O22456-1]
NP_850953.1, NM_180622.3 [O22456-2]
UniGeneiAt.24086

Genome annotation databases

EnsemblPlantsiAT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2]
AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1]
GeneIDi839040
GrameneiAT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2]
AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1]
KEGGiath:AT1G24260

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSEP3_ARATH
AccessioniPrimary (citable) accession number: O22456
Secondary accession number(s): Q8GWQ4, Q8L9R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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