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O22456

- SEP3_ARATH

UniProt

O22456 - SEP3_ARATH

Protein

Developmental protein SEPALLATA 3

Gene

SEP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development.

    GO - Molecular functioni

    1. DNA binding Source: TAIR
    2. protein binding Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: TAIR

    GO - Biological processi

    1. cell fate specification Source: TAIR
    2. flower development Source: TAIR
    3. ovule development Source: TAIR
    4. specification of floral organ identity Source: TAIR
    5. specification of floral organ number Source: TAIR
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Flowering, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Developmental protein SEPALLATA 3
    Alternative name(s):
    Agamous-like MADS-box protein AGL9
    Gene namesi
    Name:SEP3
    Synonyms:AGL9
    Ordered Locus Names:At1g24260
    ORF Names:F3I6.19
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G24260.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Developmental protein SEPALLATA 3PRO_0000199485Add
    BLAST

    Proteomic databases

    PaxDbiO22456.
    PRIDEiO22456.

    Expressioni

    Developmental stagei

    Expressed early during flower development within petals, stamens, and carpels.

    Gene expression databases

    GenevestigatoriO22456.

    Interactioni

    Subunit structurei

    Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP178394EBI-592020,EBI-592365
    AGL1P293815EBI-592020,EBI-592304
    AGL11Q388363EBI-592020,EBI-592343
    AP1P356314EBI-592020,EBI-592003
    PIP480073EBI-592020,EBI-1395334
    SEUQ8W2342EBI-592020,EBI-1771131
    SHP2Q5XXE73EBI-592020,EBI-592328
    TT16Q8RYD97EBI-592020,EBI-621993

    Protein-protein interaction databases

    BioGridi24278. 24 interactions.
    DIPiDIP-33800N.
    IntActiO22456. 19 interactions.
    STRINGi3702.AT1G24260.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22456.
    SMRiO22456. Positions 2-70.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 5755MADS-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini91 – 18191K-boxPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili94 – 17784Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 K-box domain.PROSITE-ProRule annotation
    Contains 1 MADS-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5068.
    HOGENOMiHOG000155301.
    InParanoidiO22456.
    OMAiPLECEPI.
    PhylomeDBiO22456.

    Family and domain databases

    InterProiIPR002487. TF_Kbox.
    IPR002100. TF_MADSbox.
    [Graphical view]
    PfamiPF01486. K-box. 1 hit.
    PF00319. SRF-TF. 1 hit.
    [Graphical view]
    PRINTSiPR00404. MADSDOMAIN.
    SMARTiSM00432. MADS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55455. SSF55455. 1 hit.
    PROSITEiPS51297. K_BOX. 1 hit.
    PS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN    50
    RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK 100
    LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM 150
    LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH 200
    QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS 250
    I 251
    Length:251
    Mass (Da):29,066
    Last modified:January 1, 1998 - v1
    Checksum:i0057CABD3F1AFC40
    GO
    Isoform 2 (identifier: O22456-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         90-90: Missing.

    Note: May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:250
    Mass (Da):28,967
    Checksum:iF1AA1EA27D82B63F
    GO

    Sequence cautioni

    The sequence BAC43290.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei90 – 901Missing in isoform 2. 1 PublicationVSP_008893

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015552 mRNA. Translation: AAB67832.1.
    AC002396 Genomic DNA. Translation: AAC00586.1.
    CP002684 Genomic DNA. Translation: AEE30502.1.
    CP002684 Genomic DNA. Translation: AEE30503.1.
    AY088272 mRNA. Translation: AAM65812.1.
    AK118696 mRNA. Translation: BAC43290.1. Different initiation.
    PIRiT00656.
    RefSeqiNP_564214.2. NM_102272.3. [O22456-1]
    NP_850953.1. NM_180622.2. [O22456-2]
    UniGeneiAt.24086.

    Genome annotation databases

    EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
    GeneIDi839040.
    KEGGiath:AT1G24260.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015552 mRNA. Translation: AAB67832.1 .
    AC002396 Genomic DNA. Translation: AAC00586.1 .
    CP002684 Genomic DNA. Translation: AEE30502.1 .
    CP002684 Genomic DNA. Translation: AEE30503.1 .
    AY088272 mRNA. Translation: AAM65812.1 .
    AK118696 mRNA. Translation: BAC43290.1 . Different initiation.
    PIRi T00656.
    RefSeqi NP_564214.2. NM_102272.3. [O22456-1 ]
    NP_850953.1. NM_180622.2. [O22456-2 ]
    UniGenei At.24086.

    3D structure databases

    ProteinModelPortali O22456.
    SMRi O22456. Positions 2-70.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24278. 24 interactions.
    DIPi DIP-33800N.
    IntActi O22456. 19 interactions.
    STRINGi 3702.AT1G24260.2-P.

    Proteomic databases

    PaxDbi O22456.
    PRIDEi O22456.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G24260.2 ; AT1G24260.2 ; AT1G24260 . [O22456-1 ]
    GeneIDi 839040.
    KEGGi ath:AT1G24260.

    Organism-specific databases

    GeneFarmi 3497. 352.
    TAIRi AT1G24260.

    Phylogenomic databases

    eggNOGi COG5068.
    HOGENOMi HOG000155301.
    InParanoidi O22456.
    OMAi PLECEPI.
    PhylomeDBi O22456.

    Gene expression databases

    Genevestigatori O22456.

    Family and domain databases

    InterProi IPR002487. TF_Kbox.
    IPR002100. TF_MADSbox.
    [Graphical view ]
    Pfami PF01486. K-box. 1 hit.
    PF00319. SRF-TF. 1 hit.
    [Graphical view ]
    PRINTSi PR00404. MADSDOMAIN.
    SMARTi SM00432. MADS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55455. SSF55455. 1 hit.
    PROSITEi PS51297. K_BOX. 1 hit.
    PS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Arabidopsis AGL9 MADS box gene is expressed in young flower primordia."
      Mandel M.A., Yanofsky M.F.
      Sex. Plant Reprod. 11:22-28(1998)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Landsberg erecta.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 114-251.
      Strain: cv. Columbia.
    6. "Specific interactions between the K domains of AG and AGLs, members of the MADS domain family of DNA binding proteins."
      Fan H.-Y., Hu Y., Tudor M., Ma H.
      Plant J. 12:999-1010(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "B and C floral organ identity functions require SEPALLATA MADS-box genes."
      Pelaz S., Ditta G.S., Baumann E., Wisman E., Yanofsky M.F.
      Nature 405:200-203(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, DISRUPTION PHENOTYPE.
    8. "Complexes of MADS-box proteins are sufficient to convert leaves into floral organs."
      Honma T., Goto K.
      Nature 409:525-529(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    9. Cited for: INTERACTION WITH AGL16.

    Entry informationi

    Entry nameiSEP3_ARATH
    AccessioniPrimary (citable) accession number: O22456
    Secondary accession number(s): Q8GWQ4, Q8L9R5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3