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Protein

Developmental protein SEPALLATA 3

Gene

SEP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development.

GO - Molecular functioni

  • DNA binding Source: TAIR
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • cell fate specification Source: TAIR
  • flower development Source: TAIR
  • MAPK cascade Source: InterPro
  • plant ovule development Source: TAIR
  • positive regulation of transcription from RNA polymerase II promoter Source: InterPro
  • specification of floral organ identity Source: TAIR
  • specification of floral organ number Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein SEPALLATA 3
Alternative name(s):
Agamous-like MADS-box protein AGL9
Gene namesi
Name:SEP3
Synonyms:AGL9
Ordered Locus Names:At1g24260
ORF Names:F3I6.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G24260.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001994851 – 251Developmental protein SEPALLATA 3Add BLAST251

Proteomic databases

PaxDbiO22456.

Expressioni

Developmental stagei

Expressed early during flower development within petals, stamens, and carpels.

Gene expression databases

ExpressionAtlasiO22456. baseline and differential.
GenevisibleiO22456. AT.

Interactioni

Subunit structurei

Heterodimer with AP1 or AG capable of binding to CArG-box sequences. Binds AP3/PI to form a ternary complex. Interacts with AGL16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP178394EBI-592020,EBI-592365
AGL1P293815EBI-592020,EBI-592304
AGL11Q388363EBI-592020,EBI-592343
AP1P356314EBI-592020,EBI-592003
PIP480073EBI-592020,EBI-1395334
SEUQ8W2342EBI-592020,EBI-1771131
SHP2Q5XXE73EBI-592020,EBI-592328
TT16Q8RYD97EBI-592020,EBI-621993

Protein-protein interaction databases

BioGridi24278. 25 interactors.
DIPiDIP-33800N.
IntActiO22456. 19 interactors.
STRINGi3702.AT1G24260.2.

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi85 – 114Combined sources30
Turni115 – 117Combined sources3
Helixi125 – 168Combined sources44
Helixi169 – 172Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OX0X-ray2.49A/B/C/D75-178[»]
ProteinModelPortaliO22456.
SMRiO22456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 57MADS-boxPROSITE-ProRule annotationAdd BLAST55
Domaini91 – 181K-boxPROSITE-ProRule annotationAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili94 – 177Sequence analysisAdd BLAST84

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiO22456.
OMAiSGQSMPK.
OrthoDBiEOG09360JC8.
PhylomeDBiO22456.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK
110 120 130 140 150
LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM
160 170 180 190 200
LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH
210 220 230 240 250
QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS

I
Length:251
Mass (Da):29,066
Last modified:January 1, 1998 - v1
Checksum:i0057CABD3F1AFC40
GO
Isoform 2 (identifier: O22456-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     90-90: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:250
Mass (Da):28,967
Checksum:iF1AA1EA27D82B63F
GO

Sequence cautioni

The sequence BAC43290 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00889390Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRiT00656.
RefSeqiNP_564214.2. NM_102272.4. [O22456-1]
NP_850953.1. NM_180622.3. [O22456-2]
UniGeneiAt.24086.

Genome annotation databases

EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneIDi839040.
GrameneiAT1G24260.2; AT1G24260.2; AT1G24260.
KEGGiath:AT1G24260.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015552 mRNA. Translation: AAB67832.1.
AC002396 Genomic DNA. Translation: AAC00586.1.
CP002684 Genomic DNA. Translation: AEE30502.1.
CP002684 Genomic DNA. Translation: AEE30503.1.
AY088272 mRNA. Translation: AAM65812.1.
AK118696 mRNA. Translation: BAC43290.1. Different initiation.
PIRiT00656.
RefSeqiNP_564214.2. NM_102272.4. [O22456-1]
NP_850953.1. NM_180622.3. [O22456-2]
UniGeneiAt.24086.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4OX0X-ray2.49A/B/C/D75-178[»]
ProteinModelPortaliO22456.
SMRiO22456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24278. 25 interactors.
DIPiDIP-33800N.
IntActiO22456. 19 interactors.
STRINGi3702.AT1G24260.2.

Proteomic databases

PaxDbiO22456.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G24260.2; AT1G24260.2; AT1G24260. [O22456-1]
GeneIDi839040.
GrameneiAT1G24260.2; AT1G24260.2; AT1G24260.
KEGGiath:AT1G24260.

Organism-specific databases

TAIRiAT1G24260.

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiO22456.
OMAiSGQSMPK.
OrthoDBiEOG09360JC8.
PhylomeDBiO22456.

Miscellaneous databases

PROiO22456.

Gene expression databases

ExpressionAtlasiO22456. baseline and differential.
GenevisibleiO22456. AT.

Family and domain databases

CDDicd00265. MADS_MEF2_like. 1 hit.
InterProiIPR033896. MADS_MEF2-like.
IPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEP3_ARATH
AccessioniPrimary (citable) accession number: O22456
Secondary accession number(s): Q8GWQ4, Q8L9R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.