O22415 (O22415_SAMNI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
rRNA N-glycosidase RuleBase RU004915
EC=3.2.2.22 RuleBase RU004915

Gene names

Name:

LECSNA-If EMBL AAC49989.1

Organism

Sambucus nigra (European elder) EMBL AAC49989.1

Taxonomic identifier

4202 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Dipsacales › Adoxaceae › Sambucus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. RuleBase RU004915 SAAS SAAS016138
Sequence similarities	Belongs to the ribosome-inactivating protein family. RuleBase RU004915

Ontologies

Keywords
Biological process	Plant defense RuleBase RU004915 SAAS SAAS016138
Domain	Signal EMBL AAC49989.1
Molecular function	Hydrolase RuleBase RU004915 SAAS SAAS016138 Protein synthesis inhibitor SAAS SAAS016138 RuleBase RU004915 Toxin RuleBase RU004915 SAAS SAAS016138
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential EMBL AAC49989.1
	Chain	29 – 570	542	Potential EMBL AAC49989.1		PRO_5000053324
	Chain	29 – 308	280	type 2 ribosome-inactivating protein SNAIf, A chain EMBL AAC49989.1		PRO_5000053325
	Chain	309 – 570	262	type 2 ribosome-inactivating protein SNAIf, B chain EMBL AAC49989.1		PRO_5000053326

Sequences

	Sequence		Length	Mass (Da)
	O22415 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7D799D3A20764BA6	FASTA	570	62,752
	10 20 30 40 50 60 MRVVTKLLYL VVLAICGLGI HGALTHTRVT PPVYPSVSFN LTGADTYGPF LRALQEKVIL 70 80 90 100 110 120 GNHTAFDLPV LNPESQVSDS NRFVLVPLTN PSGDTVTLAI DVVNLYVVAF SSNGRSYFFS 130 140 150 160 170 180 GSTAVQRDNL FVDTTQEELN FTGNYISLER QVGFGRVYIP LGPKSLAQAI SSLRTYTLSA 190 200 210 220 230 240 GDTKPLARGL LVVIQMVSEA ARFRYIELRI RTSITDASEF TPDLLMLSME NNWSSMSSEI 250 260 270 280 290 300 QQAQPGGIFP GVVQLRDERN NPIEVTNFRR LFELTYIAVL LYGCAPVTSN SYTNNAIDAQ 310 320 330 340 350 360 IIKMPVFRGG GYEKVCSVVE VTRRISGWDG LCVDVRDGHY IDGNTVQLGP CGNECNQLWT 370 380 390 400 410 420 FRTDGTIRWL GKCLTTSSSV MIYDCNTVPP EATKWVVSTD GTITNPRSGL VLTAPQAAEG 430 440 450 460 470 480 TALSLENNIH AARQGWTVGD VEPLVTFIVG YKQMCLTENG ENNFVWLEDC VLNRVEQEWA 490 500 510 520 530 540 LYGDGTIRVN SNRSLCVTSE DHEPSDLIVI LKCEGSGNQR WVFNTNGTIS NPNAKLVMDV 550 560 570 AQSNVSLRKI ILYPPTGNPN QQWITTTQPA

References

[1]

"Elderberry (Sambucus nigra) contains truncated Neu5Ac(alpha-2,6)Gal/GalNAc-binding type 2 ribosome-inactivating proteins." Peumans W.J., Roy S., Barre A., Rouge P., van Leuven F., van Damme E.J. FEBS Lett. 425:35-39(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Fruit EMBL AAC49989.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF012899 mRNA. Translation: AAC49989.1.
3D structure databases
HSSP	HSSP built from PDB template 1HWM based on UniProtKB Q9AVR2.
ProteinModelPortal	O22415.
SMR	O22415. Positions 33-285, 315-566.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.420.10. 1 hit. 4.10.470.10. 1 hit.
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00652. Ricin_B_lectin. 2 hits. PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
SMART	SM00458. RICIN. 2 hits. [Graphical view]
SUPFAM	SSF56371. Ribosome_inactivat_prot. 1 hit. SSF50370. RicinB_like. 2 hits.
PROSITE	PS50231. RICIN_B_LECTIN. 2 hits. PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O22415_SAMNI

Accession

Primary (citable) accession number: O22415

Entry history

Integrated into UniProtKB/TrEMBL:	January 1, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)