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O22397 (POT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium transporter 1
Short name=AtKT1
Short name=AtKUP1
Short name=AtPOT1
Gene names
Name:POT1
Synonyms:KT1, KUP1
Ordered Locus Names:At2g30070
ORF Names:F23F1.18, T27E13.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity potassium transporter that could play a major role in the uptake of potassium from the rhizosphere. May act as a low-affinity potassium transporter under high potassium concentrations. Could also transport rubidium. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Tissue specificity

Detected in the whole mature plant but preferentially expressed in roots and stems, and in potassium-starved plants. Ref.2

Miscellaneous

Low-affinity activity is strongly inhibited by barium or cesium.

Sequence similarities

Belongs to the HAK/KUP transporter (TC 2.A.72.3) family. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionpotassium ion transmembrane transporter activity

Inferred from sequence or structural similarity Ref.1. Source: TAIR

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O22397-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O22397-2)

The sequence of this isoform differs from the canonical sequence as follows:
     16-31: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 712712Potassium transporter 1
PRO_0000209077

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 6727Extracellular Potential
Transmembrane68 – 8821Helical; Potential
Topological domain89 – 15466Cytoplasmic Potential
Transmembrane155 – 17521Helical; Potential
Topological domain176 – 18914Extracellular Potential
Transmembrane190 – 21021Helical; Potential
Topological domain211 – 2199Cytoplasmic Potential
Transmembrane220 – 24021Helical; Potential
Topological domain241 – 26727Extracellular Potential
Transmembrane268 – 28821Helical; Potential
Topological domain289 – 30012Cytoplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 34019Extracellular Potential
Transmembrane341 – 36121Helical; Potential
Topological domain362 – 39231Cytoplasmic Potential
Transmembrane393 – 41321Helical; Potential
Topological domain414 – 42411Extracellular Potential
Transmembrane425 – 44521Helical; Potential
Topological domain446 – 4494Cytoplasmic Potential
Transmembrane450 – 47021Helical; Potential
Topological domain471 – 4744Extracellular Potential
Transmembrane475 – 49521Helical; Potential
Topological domain496 – 712217Cytoplasmic Potential

Natural variations

Alternative sequence16 – 3116Missing in isoform 2.
VSP_008984

Experimental info

Sequence conflict1001F → L in AAC49844. Ref.1
Sequence conflict1801L → P in AAC49844. Ref.1
Sequence conflict3191A → V in AAC49844. Ref.1
Sequence conflict6241E → G in AAC49844. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 21, 2003. Version 2.
Checksum: 0FE32EE88807536D

FASTA71279,127
        10         20         30         40         50         60 
MNQSPSLIEQ GISQQHLKTL SCANVLTLAY QSLGVIYGDL STSPLYVYKT TFSGKLSLHE 

        70         80         90        100        110        120 
DDEEIFGVFS FIFWTFTLIA LFKYVFIVLS ADDNGEGGTF ALYSLLCRYA KLSILPNHQE 

       130        140        150        160        170        180 
MDEKLSTYAT GSPGETRQSA AVKSFFEKHP KSQKCLLLFV LLGTCMAIGD SVLTPTISVL 

       190        200        210        220        230        240 
SAVSGVKLKI PNLHENYVVI IACIILVAIF SVQRYGTHRV AFIFAPISTA WLLSISSIGV 

       250        260        270        280        290        300 
YNTIKWNPRI VSALSPVYMY KFLRSTGVEG WVSLGGVVLS ITGVETMFAD LGHFSSLSIK 

       310        320        330        340        350        360 
VAFSFFVYPC LILAYMGEAA FLSKHHEDIQ QSFYKAIPEP VFWPVFIVAT FAAVVGSQAV 

       370        380        390        400        410        420 
ISATFSIISQ CCALDCFPRV KIIHTSSKIH GQIYIPEVNW MLMCLCLAVT IGLRDTNMMG 

       430        440        450        460        470        480 
HAYGLAVTSV MLVTTCLMTL VMTIVWKQRI ITVLAFVVFF GSIELLYFSS CVYKVPEGGW 

       490        500        510        520        530        540 
IPILLSLTFM AVMYIWNYGT TKKHEFDVEN KVSMDRIVSL GPSIGMVRVP GIGLVYSNLV 

       550        560        570        580        590        600 
TGVPAVFGHF VTNLPAFHKI LVFVCVKSVQ VPYVGEEERF VISRVGPKEY GMFRSVVRYG 

       610        620        630        640        650        660 
YRDVPREMYD FESRLVSAIV EFVETEPGLE EEEMSSVRRK KEECMEIMEA KEAGVAYILG 

       670        680        690        700        710 
HSYAKAKQSS SVLKKLAVNV VFAFMSTNCR GTDVVLNVPH TSLLEVGMVY YV

« Hide

Isoform 2 [UniParc].

Checksum: 82DEC62AD79229DD
Show »

FASTA69677,370

References

« Hide 'large scale' references
[1]"A new family of K+ transporters from Arabidopsis that are conserved across phyla."
Quintero F.J., Blatt M.R.
FEBS Lett. 415:206-211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), CHARACTERIZATION.
Strain: cv. Columbia.
[2]"AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport activity."
Kim E.J., Kwak J.M., Uozumi N., Schroeder J.I.
Plant Cell 10:51-62(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[3]"AtKUP1: a dual-affinity K+ transporter from Arabidopsis."
Fu H.-H., Luan S.
Plant Cell 10:63-73(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION.
Strain: cv. Columbia.
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Phylogenetic relationships within cation transporter families of Arabidopsis."
Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H., Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F., Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.
Plant Physiol. 126:1646-1667(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF012656 Genomic DNA. Translation: AAC49844.1.
AF029876 mRNA. Translation: AAB88901.1.
AF033118 mRNA. Translation: AAB87687.1.
AC004165 Genomic DNA. Translation: AAC16965.1.
AC004680 Genomic DNA. Translation: AAM14984.1.
CP002685 Genomic DNA. Translation: AEC08341.1.
IPIIPI00540910.
IPI00545304.
PIRT02479. T00591.
RefSeqNP_180568.1. NM_128562.3.
UniGeneAt.10484.
At.66278.

3D structure databases

ProteinModelPortalO22397.
ModBaseSearch...

Protein family/group databases

TCDB2.A.72.3.1. K+ uptake permease (KUP) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30070.1; AT2G30070.1; AT2G30070.
GeneID817558.
KEGGath:AT2G30070.

Organism-specific databases

GeneFarm2392.
TAIRAt2g30070.

Phylogenomic databases

eggNOGCOG3158.
HOGENOMHOG000218032.
InParanoidO22397.
KOK03549.
OMAIGHETIF.
PhylomeDBO22397.
ProtClustDBCLSN2683851.

Enzyme and pathway databases

BioCycARA:AT2G30070-MONOMER.
MetaCyc:MONOMER-14583.

Gene expression databases

ArrayExpressO22397.
GenevestigatorO22397.
GermOnlineAT2G30070. Arabidopsis thaliana.

Family and domain databases

InterProIPR003855. K+_transporter.
[Graphical view]
PfamPF02705. K_trans. 1 hit.
[Graphical view]
TIGRFAMsTIGR00794. kup. 1 hit.
ProtoNetSearch...

Entry information

Entry namePOT1_ARATH
AccessionPrimary (citable) accession number: O22397
Secondary accession number(s): O50043
Entry history
Integrated into UniProtKB/Swiss-Prot: November 21, 2003
Last sequence update: November 21, 2003
Last modified: May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents