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Protein

Serine/arginine-rich-splicing factor SR34

Gene

SR34

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General splicing factor. Can promote splice site selection in vitro presumably by antagonizing the effects of the A1 heterogeneous nuclear ribonucleoprotein. May have an essential function during early plant development.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-72165. mRNA Splicing - Minor Pathway.
R-ATH-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich-splicing factor SR34
Short name:
At-SR34
Short name:
At-SRp34
Short name:
AtSR34
Alternative name(s):
Pre-mRNA-splicing factor SF2
SR1 protein
Gene namesi
Name:SR34
Synonyms:SF2, SR1, SRP34
Ordered Locus Names:At1g02840
ORF Names:F22D16.16, F22D16_30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02840.

Subcellular locationi

GO - Cellular componenti

  • interchromatin granule Source: TAIR
  • nuclear speck Source: TAIR
  • nucleus Source: TAIR
  • spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Serine/arginine-rich-splicing factor SR34PRO_0000081917Add
BLAST

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22315.
PRIDEiO22315.

PTM databases

iPTMnetiO22315.

Expressioni

Tissue specificityi

Ubiquitous.3 Publications

Gene expression databases

ExpressionAtlasiO22315. baseline and differential.
GenevisibleiO22315. AT.

Interactioni

Subunit structurei

Component of the spliceosome. Interacts with SNRNP35, RS2Z33, SR34, CYP59, CYP63 and CYP95. The binding to CYP63 is phosphorylation-dependent (PubMed:15166240).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CYP63Q9LY753EBI-927464,EBI-2360522

Protein-protein interaction databases

BioGridi24497. 13 interactions.
IntActiO22315. 11 interactions.
STRINGi3702.AT1G02840.1.

Structurei

3D structure databases

ProteinModelPortaliO22315.
SMRiO22315. Positions 9-84, 113-183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 8276RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini119 – 19779RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi96 – 11217Gly-rich (hinge region)Add
BLAST
Compositional biasi198 – 27376Arg/Ser-rich (RS domain)Add
BLAST
Compositional biasi274 – 30330Lys/Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family. SR subfamily.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0105. Eukaryota.
COG0724. LUCA.
InParanoidiO22315.
KOiK12890.
OMAiYTCYEDM.
OrthoDBiEOG09360OW1.
PhylomeDBiO22315.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O22315-1) [UniParc]FASTAAdd to basket
Also known as: SF2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRSSRTVY VGNLPGDIRE REVEDLFSKY GPVVQIDLKV PPRPPGYAFV
60 70 80 90 100
EFDDARDAED AIHGRDGYDF DGHRLRVELA HGGRRSSDDT RGSFNGGGRG
110 120 130 140 150
GGRGRGDGGS RGPSRRSEFR VLVTGLPSSA SWQDLKDHMR KGGDVCFSQV
160 170 180 190 200
YRDARGTTGV VDYTCYEDMK YALKKLDDTE FRNAFSNGYV RVREYDSRKD
210 220 230 240 250
SRSPSRGRSY SKSRSRSRGR SVSRSRSRSR SRSRSPKAKS SRRSPAKSTS
260 270 280 290 300
RSPGPRSKSR SPSPRRSRSR SRSPLPSVQK EGSKSPSKPS PAKSPIHTRS

PSR
Length:303
Mass (Da):33,729
Last modified:January 1, 1998 - v1
Checksum:i6530F9CB628B8EFA
GO
Isoform 2 (identifier: O22315-2) [UniParc]FASTAAdd to basket
Also known as: SF2B

The sequence of this isoform differs from the canonical sequence as follows:
     267-272: SRSRSR → YGFTYD
     273-303: Missing.

Show »
Length:272
Mass (Da):30,509
Checksum:i3EDCF085D2003C05
GO
Isoform 3 (identifier: O22315-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     267-285: SRSRSRSPLPSVQKEGSKS → WITVETLDHLDHNIISGFL
     286-303: Missing.

Show »
Length:285
Mass (Da):31,967
Checksum:i85FC1FCAD24C9BF6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1251G → W in AAA32856 (PubMed:7644475).Curated
Sequence conflicti127 – 1271P → A in AAA32856 (PubMed:7644475).Curated
Sequence conflicti139 – 1402MR → IA in AAA32856 (PubMed:7644475).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei267 – 28519SRSRS…EGSKS → WITVETLDHLDHNIISGFL in isoform 3. 1 PublicationVSP_054984Add
BLAST
Alternative sequencei267 – 2726SRSRSR → YGFTYD in isoform 2. CuratedVSP_005859
Alternative sequencei273 – 30331Missing in isoform 2. CuratedVSP_005860Add
BLAST
Alternative sequencei286 – 30318Missing in isoform 3. 1 PublicationVSP_054985Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98340 mRNA. Translation: AAA32856.1.
AF001035 Genomic DNA. Translation: AAB71385.1.
AF001035 Genomic DNA. Translation: AAB71386.1.
AC009525 Genomic DNA. Translation: AAF02881.1.
CP002684 Genomic DNA. Translation: AEE27477.1.
CP002684 Genomic DNA. Translation: AEE27478.1.
CP002684 Genomic DNA. Translation: AEE27479.1.
AK118379 mRNA. Translation: BAC42991.1.
AY128338 mRNA. Translation: AAM91541.1.
BT006316 mRNA. Translation: AAP13424.1.
AY085920 mRNA. Translation: AAM63132.1.
PIRiF86158.
S71185.
RefSeqiNP_563665.3. NM_100165.3. [O22315-1]
NP_850933.1. NM_180602.1. [O22315-1]
NP_850934.1. NM_180603.1. [O22315-3]
UniGeneiAt.24497.

Genome annotation databases

EnsemblPlantsiAT1G02840.1; AT1G02840.1; AT1G02840. [O22315-1]
AT1G02840.2; AT1G02840.2; AT1G02840. [O22315-3]
AT1G02840.3; AT1G02840.3; AT1G02840. [O22315-1]
GeneIDi839262.
KEGGiath:AT1G02840.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98340 mRNA. Translation: AAA32856.1.
AF001035 Genomic DNA. Translation: AAB71385.1.
AF001035 Genomic DNA. Translation: AAB71386.1.
AC009525 Genomic DNA. Translation: AAF02881.1.
CP002684 Genomic DNA. Translation: AEE27477.1.
CP002684 Genomic DNA. Translation: AEE27478.1.
CP002684 Genomic DNA. Translation: AEE27479.1.
AK118379 mRNA. Translation: BAC42991.1.
AY128338 mRNA. Translation: AAM91541.1.
BT006316 mRNA. Translation: AAP13424.1.
AY085920 mRNA. Translation: AAM63132.1.
PIRiF86158.
S71185.
RefSeqiNP_563665.3. NM_100165.3. [O22315-1]
NP_850933.1. NM_180602.1. [O22315-1]
NP_850934.1. NM_180603.1. [O22315-3]
UniGeneiAt.24497.

3D structure databases

ProteinModelPortaliO22315.
SMRiO22315. Positions 9-84, 113-183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24497. 13 interactions.
IntActiO22315. 11 interactions.
STRINGi3702.AT1G02840.1.

PTM databases

iPTMnetiO22315.

Proteomic databases

PaxDbiO22315.
PRIDEiO22315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02840.1; AT1G02840.1; AT1G02840. [O22315-1]
AT1G02840.2; AT1G02840.2; AT1G02840. [O22315-3]
AT1G02840.3; AT1G02840.3; AT1G02840. [O22315-1]
GeneIDi839262.
KEGGiath:AT1G02840.

Organism-specific databases

TAIRiAT1G02840.

Phylogenomic databases

eggNOGiKOG0105. Eukaryota.
COG0724. LUCA.
InParanoidiO22315.
KOiK12890.
OMAiYTCYEDM.
OrthoDBiEOG09360OW1.
PhylomeDBiO22315.

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-72165. mRNA Splicing - Minor Pathway.
R-ATH-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiO22315.

Gene expression databases

ExpressionAtlasiO22315. baseline and differential.
GenevisibleiO22315. AT.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSR34_ARATH
AccessioniPrimary (citable) accession number: O22315
Secondary accession number(s): O22314
, Q39201, Q564E1, Q8L7P1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The splicing pattern of the pre-mRNA is regulated in a tissue-specific manner and by development, and changes in response to various types of abiotic stresses and hormones (PubMed:10215626, PubMed:17319848).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.