O22308 (7OMT6_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Isoflavone-7-O-methyltransferase 6 EC=2.1.1.150 Alternative name(s): 7-IOMT-6 Isoflavone-O-methyltransferase 6
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transfers a methyl group to 7-hydroxyls of the isoflavones daidzein, genistein and 6,7,4'-trihydroxyisoflavone. Can also methylate (+)6a-hydroxymaackiain with lower efficiency.
Catalytic activity	S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone.
Pathway	Phytoalexin biosynthesis; medicarpin biosynthesis.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	isoflavone 7-O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Isoflavone-7-O-methyltransferase 6		PRO_0000204436
Regions
	Region	118 – 127	10	Substrate binding By similarity
Sites
	Active site	257	1	Proton acceptor
	Binding site	196	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	219	1	S-adenosyl-L-methionine By similarity
	Binding site	239	1	S-adenosyl-L-methionine By similarity
	Binding site	240	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	253	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O22308 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 31B95226966C1C98	FASTA	352	39,605
	10 20 30 40 50 60 MASSINGRKP SEIFKAQALL YKHIYAFIDS MSLKWAVEMN IPNIIQNHGK PISLSNLVSI 70 80 90 100 110 120 LQVPSSKIGN VRRLMRYLAH NGFFEIITKE EESYALTVAS ELLVRGSDLC LAPMVECVLD 130 140 150 160 170 180 PTLSGSYHEL KKWIYEEDLT LFGVTLGSGF WDFLDKNPEY NTSFNDAMAS DSKLINLALR 190 200 210 220 230 240 DCDFVFDGLE SIVDVGGGTG TTAKIICETF PKLKCIVFDR PQVVENLSGS NNLTYVGGDM 250 260 270 280 290 300 FTSIPNADAV LLKYILHNWT DKDCLRILKK CKEAVTNDGK RGKVTIIDMV IDEKKDENQV 310 320 330 340 350 TQIKLLMDVN MACLNGKERN EEEWKKLFIE AGFQHYKISP LTGFLSLIEI YP

References

[1]	"Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase." He X.-Z., Reddy J.T., Dixon R.A. Plant Mol. Biol. 36:43-54(1998) [PubMed: 9484461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF000975 mRNA. Translation: AAC49926.1.
3D structure databases
ProteinModelPortal	O22308.
SMR	O22308. Positions 8-352.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

7OMT6_MEDSA

Accession

Primary (citable) accession number: O22308

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program